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Citations for Sartorius :
151 - 200 of 232 citations for 6 METHYL 6 HEPTEN 4 YN 2 OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm, Sartorius Stedim Biotech GmbH). The biomass was washed on the filter with 10 mL of 60% methanol and stored at -70 °C until metabolite extraction.
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Cancer Biology 2022Quote: ... Medium containing DMSO or increasing concentration of test compound was added to each well and spheroid growth was monitored for 4 days post-treatment using the IncuCyte® Spheroid Analysis system (Sartorius). Images from 4 days post-treatment and day 0 were analyzed using the IncuCyte® Spheroid Analysis software module ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was applied to 2 kDa centrifugal concentrators (Vivaspin 15 R Hydrosart filter device, Sartorius, Germany) and centrifuged for 1 h at 3000 x g according to the suppliers’ protocol ...
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bioRxiv - Microbiology 2022Quote: ... The samples were then concentrated from 40 mL to ∼2 mL using vivaspin 20 ultrafiltration units (Sartorius), and further concentrated to ∼100 µL using vivaspin 2 ultrafiltration units (Sartorius) ...
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bioRxiv - Microbiology 2022Quote: ... The samples were first concentrated from 2 L down to 40 mL using the vivaflow 200 (Sartorius). The samples were then concentrated from 40 mL to ∼2 mL using vivaspin 20 ultrafiltration units (Sartorius) ...
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bioRxiv - Cell Biology 2022Quote: ... the Spectrum polyP was desalted using a Vivacon 500 2 kDa cutoff spin filter (Sartorius, Bohemia, NY) at 7500 x g for 60 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The medium was filter sterilized using a 0.2 μm Sartopore 2 filter unit (Sartorius Stedim, Goettingen, Germany).
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bioRxiv - Immunology 2022Quote: ... followed by the removal of 2-MEA by buffer-exchanging to PBS using 100kDa Vivaspin6 columns (Sartorius) and storing overnight at 4 °C to allow for reoxidation of the disulfide bonds.
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bioRxiv - Synthetic Biology 2023Quote: ... The cell culture was inoculated at OD600 0.3 in the 2 L bioreactor (Biostat B, Sartorius, Germany) including the 1 L modified M9 minimal medium ...
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bioRxiv - Microbiology 2024Quote: ... A global association 1:2 bivalent analyte model using the Octet Data Analysis HT software v7 (Sartorius) was used to account for the dimeric HAstV-VA1 spike ...
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bioRxiv - Microbiology 2019Quote: ... viruses were concentrated by centrifugation at 4 °C using vivaspin 20 concentrators with a molecular weight cutoff of 100K (Sartorius, Göttingen, Germany). Thereafter ...
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bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
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bioRxiv - Microbiology 2020Quote: ... liquid phage suspension was centrifuged at 4000 g for 10 min at 4°C and the supernatant was subsequently filter sterilised using a 0.45 μm pore sized membrane filter (Sartorius Minisart®, 16555). The filtrate phage lysate was stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: The purified proteins were concentrated and exchanged to 10 mM sodium citrate buffer (pH 5.0) using Vivaspin® Turbo 4 ultrafiltration units with a 5000 MWCO PES membrane (Sartorius; Cat.# VS04T11). The purity and concentration of each PcaLOOL was assessed by SDS-PAGE and the Pierce™ BCA Protein Assay Kit ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was concentrated by centrifugation for 1 hour at 3220 g at 4°C in a 5kDa Cutoff Vivaspin tube (Sartorius, Cat # VS2012). ACM was aliquoted into low protein binding tubes (∼100ul per aliquot) ...
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bioRxiv - Microbiology 2021Quote: ... Flow-through and wash fractions were pooled and concentrated using Vivaspin 15R concentrators (2 kDa MWCO HY, Sartorius).
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bioRxiv - Molecular Biology 2021Quote: ... diluted to a final Env trimer concentration of 2 mg/mL and filtered using 0.45 + 0.2 µm Sartobran 300 filter capsules (Sartorius). As final steps ...
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bioRxiv - Immunology 2020Quote: ... GFP fluorescent intensity was detected every 2 hours using the IncuCyte S3 Live-Cell analysis system (Sartorius, Germany). Total integrated GFP intensity per well was used as a quantitative measure of viable target cells ...
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bioRxiv - Biochemistry 2020Quote: ... Obtained fractions were analyzed by SDS PAGE and fractions containing proKLK14 were concentrated with Vivaspin® 2 (Sartorius) and further purified on Superdex s75 pg (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Parental and MCT1-KO MIA PaCa-2 cells were transduced with Lentiviral NucLight Red and Green vectors (Sartorius), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing FimH-FimC were dialyzed against buffer A and were concentrated using Vivaspin 2 (Sartorius, Göttingen, Germany). For cryo-EM ...
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bioRxiv - Immunology 2024Quote: The binding kinetics of ZBP1-Zα1Zα2 with SARS-CoV-2 RNAs were evaluated using the Octet RED96 instrument (Sartorius). First ...
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bioRxiv - Plant Biology 2019Quote: ... These filtrates were ultra-filtered at 4°C through 20-ml Vivaspin micro-concentrators (10,000 molecular weight cut off, Sartorius Stedim, Biotech GmbH, Germany). Retained protein suspensions were used for protein content and enzyme assays ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Cancer Biology 2023Quote: Real-time calcein intensity was monitored using fluorescence live-cell imaging combined with a phase-contrast setup using the 4×objective (Sartorius AG, Gottingen, Germany). Images were acquired hourly and analyzed using Incucyte software and ImageJ.
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bioRxiv - Bioengineering 2020Quote: Batch experiments were performed using a 2 L bioreactor equipped with a Sartorius BIOSTAT B® fermentation controller (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Molecular Biology 2021Quote: ... Triton X-100 and incubation at RT for 60-120 mins and solutions sterile filtered using 0.45 µm Sartopore 2 filters (Sartorius). Triton X-100 inactivated solutions were batch-wise loaded onto a MAb PGT145 immunoaffinity column for capture of ConM and ConS Env trimers ...
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bioRxiv - Biophysics 2021Quote: ... Following sonication, DNA impurities were digested (DNase: Fisher Optizyme, 2 U/ml) and the supernatant was filtered (0.45 μm, Sartorius) and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: Protein-containing fractions were combined and concentrated to 0.5 ml using a 50-kDa molecular weight cutoff (MWCO) Vivaspin 2 concentrator (Sartorius), and desalted by buffer exchange on a PD MiniTrap G-25 column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... and BA.2+R493Q RBDs were diluted to 5 μg/mL and immobilized onto pre-hydrated streptavidin biosensors (Sartorius) to a level of 1 nm total shift ...
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bioRxiv - Biochemistry 2022Quote: ... samples were further concentrated to ~1-2 mg/mL with a 100 kDa MWCO Vivaspin 500 centrifugal concentrator (Sartorius) at 12000 ×g ...
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bioRxiv - Systems Biology 2023Quote: ... The wells were imaged every 2 hours for 120 hours and cell confluence was measured with Incucyte ZOOM (Sartorius).
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bioRxiv - Bioengineering 2022Quote: Fed-batch bioreactor experiments were performed in 2-L bench top glass fermenters (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated EVs were subsequently concentrated to a volume of 100 ul using a 2 ml 100,000 kDa Vivaspin column (Sartorius).
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bioRxiv - Microbiology 2021Quote: ... The samples were weight with scales from KERN ALS 220-4 (KERN & SOHN GmbH, Balingen-Frommern, Germany) and Sartorius Cubis®-series (Sartorius AG, Göttingen, Germany). During the extraction process ...
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bioRxiv - Physiology 2023Quote: ... Bundles were lyophilized in a freeze-dryer (Labconco, Kansas City, MO, USA) for >4 h and weighed on a microbalance (Sartorius Cubis Microbalance, Gottingen, Germany). The rate of mH2O2 emission was calculated using a standard curve under the same assay conditions and then normalized to fibre bundle dry weight.
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell monolayer was scraped and migration was monitored via time-lapse image acquisition every 2 hours via the IncuCyte ZOOM imaging platform (Sartorius). Migration rates were calculated as relative wound density over time for wildtype and mutant cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... Brightfield images were taken every 2 hours for 5 days and the percentage confluency calculated using an Incucyte Live-Cell analysis system (Sartorius).
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... Fragmented gDNA was obtained by beat-beating the intact gDNA for 12 minutes using 2 mm diameter glass beads in a Mikro-Dismembrator U (Sartorius) at 2000 rpm ...
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bioRxiv - Immunology 2020Quote: ... The EV pellet was resuspended in 2 ml PBS and stored at −80 prior to further concentration using a 5 kDa MW cut-off vivaspin (Sartorius). The protein content of EVs was quantified using Qubit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Images and confluence data were acquired every 2 h for ~200 h using the IncuCyte Live Cell Analysis Imaging System (Sartorius). The Area Under Curve (AUC ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Cell Biology 2023Quote: Isolated ECs were seeded onto 96 well-plates pre-coated with fibronectin in EGM-2 and the proliferation assay was performed for 60 hours in the Incucyte proliferation assay according to (21) (Sartorius). The analysis of the cell proliferation was performed by the Incucyte Basic Analyzer software.
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bioRxiv - Bioengineering 2022Quote: ... Four images were recorded per well every 2 h and analyzed using IncuCyte cell by cell analysis software module (Sartorius). The killing potency of the CAR-T cells was assessed by comparing the percentage of Annexin V positive target cells over time relative to the total number of target cells.
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bioRxiv - Bioengineering 2023Quote: ... This experiment was carried out in a commercial stirred tank bioreactor (STBR) of 2 l total volume (Sartorius AG, Germany) and a working volume of 1 l was used ...