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Citations for Sartorius :
51 - 100 of 321 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The eluted complex was then concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius). A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... GO16 KS3 was cultivated in a 5 L stirred tank bioreactor (Sartorius UniVessel Glass 5 l) containing 3 L of MSMlim broth ...
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bioRxiv - Biochemistry 2020Quote: ... or Vivaspin (Sartorius, MWCO 5 kDa) concentration columns.
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bioRxiv - Bioengineering 2020Quote: Batch experiments were performed using a 2 L bioreactor equipped with a Sartorius BIOSTAT B® fermentation controller (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2020Quote: Aerobic fermentor growth of ΔssaACB cell culture was achieved using a BIOSTAT® B bioreactor (Sartorius Stedim, Göttingen, Germany) and samples were collected as described in Puccio and Kitten (2020) ...
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bioRxiv - Biochemistry 2022Quote: ... and then the supernatant was concentrated and exchanged into Buffer B (25 mM HEPES pH 7.5 and 150 mM NaCl) using a Hydrosart Ultrafilter system (Sartorius). The following purification steps for anti-BRIL Fab were the same as that of BRIL-SLC19A1 ...
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bioRxiv - Bioengineering 2022Quote: Fed-batch bioreactor experiments were performed in 2-L bench top glass fermenters (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Immunology 2023Quote: ... the mNeonGreen signals from infected MRC-5 cells were monitored for 1 week using the Incucyte™ Live-Cell analysis system (Sartorius). Additionally ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsamples were weighed (20 mg ± 6 mg) on a fine scale balance (Entris, Sartorius). Together with 6 negative controls (200 μl lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... the samples were concentrated using filter columns (Vivaspin 6, 100,000 MWCO PES, Sartorius, Germany) at 4 °C with a maximal speed of 4,000 x g and washed twice with DBCM2 salt solution ...
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bioRxiv - Microbiology 2020Quote: ... with buffer B Purity was checked via SDS-PAGE with Coomassie Staining and concentration was performed using Vivaspin Turbo apparatus (Sartorius) with a 5 kDa molecular weight cut-off.
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bioRxiv - Microbiology 2023Quote: ... at a shaking speed of 150 rpm and used to start the batch phase in a lab-scale stirred bioreactor (Biostat B-Twin, Sartorius) total volume ...
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bioRxiv - Biochemistry 2022Quote: The bioreactor cell culture experiments were conducted in a 3L glass bioreactor using Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 1.75L ...
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bioRxiv - Plant Biology 2024Quote: ... FLVB and FLVA/B were analyzed by SDS-page and then concentrated by ultra filtration (Sartorius, Vivaspin, 10 kDa cutoff). Analysis of different purification steps and quality of protein purifications was also assessed by Immunoblot analysis with custom-made α-FLVA ...
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bioRxiv - Immunology 2019Quote: ... Worm culture supernatants collected over 1 week were further concentrated using centrifugal protein concentrators with a 5 kDa MWCO (Vivaspin, Sartorius, Göttingen, Germany) to obtain the final ...
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bioRxiv - Plant Biology 2021Quote: ... and 5-μm syringe filter (Sartorius, Germany) to remove RLX (0 ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was then concentrated using Vivaspin® 6 100,000 MWCO concentrator columns (Sartorius). The sample was then purified via size exclusion chromatography using a Superose™ 6 Increase column (GE Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Immunology 2022Quote: ... and the eluate was collected into a Vivaspin 6 50 kDa MWCO column (Sartorius, V10631), which was centrifugated at 2,113 g for five minutes to concentrate the antibodies ...
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bioRxiv - Immunology 2021Quote: ... 7 L of Sf9 cell culture at a density of 2 × 106 cells/mL were infected with the baculovirus at a MOI of 3 using a Biostat B plus bioreactor (Sartorius, Germany). The following conditions were maintained during the culture period ...
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bioRxiv - Microbiology 2020Quote: Batch and fed-batch bioreactor experiments were performed in 2 L bench top glass fermentors (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Biophysics 2020Quote: ... were collected and concentrated using a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius) to a final concentration ∼2.5 mg mL-1 ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing Ldh-EtfAB were pooled and concentrated via ultrafiltration (Vivaspin 6, 30 kDa Cut-off, Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2023Quote: ... which took place overnight on arrival at 4°C (cold room) using 6 Vivaflow200 filtration units (Sartorius) operating in two parallel series ...
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bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM MgCl2 on BLItz machine (FortéBio Sartorius). Streptavidin coated biosensors were used in all the measurements and were hydrated in the reaction buffer for 10 minutes before loading ...
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bioRxiv - Biochemistry 2022Quote: ... 5 % CO2 in an Incucyte® Zoom (Sartorius AG) and scanned every 8 h for 2 days ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml using a Vivaspin concentrator spin column (Sartorius). The protein sample was applied to a HiLoad 16/600 Superdex 200 column (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml using a Vivaspin concentrator spin column (Sartorius) at 4,500 × g ...
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bioRxiv - Biochemistry 2020Quote: ... The 5 kDa MWCO concentrator filtrate (Sartorius, Göttingen, Germany) was used to control for the amount of unbound metal in the protein samples ...
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bioRxiv - Microbiology 2023Quote: ... concentrated using Vivaspin Turbo 5 kDa centrifugal concentrators (Sartorius). Initial crystallization hits were obtained using our in-house crystallization facility (37) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM DTT) using a 5-KD concentrator (Sartorius). The purified samples were aliquoted ...
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bioRxiv - Systems Biology 2022Quote: ... and then 6 hours after doxorubicin treatment was moved to an incubator connected to an Incucyte microscope (Sartorius). Brightfield images of wells were taken 6 ...
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bioRxiv - Cell Biology 2022Quote: ... 6 ml of the crude CM was passed through a 300 kDa Vivaspin concentrator (Sartorius AG, Göttingen, Germany) by centrifugation at 3,000 g for ∼15 minutes (or until the retentate was ∼300 µl) ...
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bioRxiv - Microbiology 2020Quote: ... Eluted proteins were concentrated using an ultrafiltration membrane with a 10 kDa molecular weight cutoff (Vivaspin 6, Sartorius). To reconstitute the BAM-DolP complex in vitro ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6.0) using a centrifugal concentrator with a molecular weight cutoff of 10 kDa (Vivaspin 6, VS0602, Sartorius). The presence of the fucosidase (62 kDa ...
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bioRxiv - Microbiology 2019Quote: ... Cultures in chemostat and segregostat mode have been performed in lab-scale stirred bioreactor (Biostat B-Twin, Sartorius. Total volume: 2L; working volume: 1L). For the batch phase ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were washed once with warm (37°C) PBS and then lifted from wells using Trypsin EDTA solution B (Sartorius, Cat# 03-052-1B) (5mins ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Neuroscience 2023Quote: ... then concentrated using a 5 kDa spin column (Sartorius, VSO611) and flash-frozen with liquid nitrogen in single-use aliquots for storage at -80 °C ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biochemistry 2021Quote: ... Purified V-ATPase in buffer containing LMNG was concentrated to 1.9 mg/mL with a 100 kDa MWCO VivaSpin 6 centrifugal concentrator at 2000 ×g for 15 min (Sartorius), followed by a VivaSpin 500 concentrator at 12,000 ×g for 2 min.
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bioRxiv - Biophysics 2023Quote: ... Fractions containing Ub were concentrated and buffer exchanged into pH 6.8 buffer (see above) using Vivaspin 6 centrifugal concentrators with a molecular weight cutoff (MWCO) of 5000 Da (Sartorius). Purified proteins were frozen at −80°C.
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bioRxiv - Microbiology 2023Quote: ... whole well images were taken at 6 h intervals using an Incucyte Zoom S3 live cell imaging system (Sartorius).
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bioRxiv - Microbiology 2023Quote: ... Total green cell count was measured every 6 h using an Incucyte Zoom S3 live cell imaging system (Sartorius) and normalised for confluency variation ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was then concentrated to the approximate concentration of 10 mg/ml using a Vivaspin 6 3,000 MWCO PES (Sartorius) membrane ultrafiltration tube.
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bioRxiv - Bioengineering 2024Quote: ... and arginine (pH 6) to a final concentration of ≥200 mg/mL using the Ambr Crossflow system (Sartorius, Germany).
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...