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Citations for Sartorius :
51 - 100 of 292 citations for 6 Chloro imidazo 1 2 a pyridine 8 carboxylic acid methyl ester hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... ACM was added and centrifuged in a swing-bucket centrifuge for 8 minutes at 4,000 g per the recommendation by Sartorius. The column was inverted ...
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bioRxiv - Biochemistry 2022Quote: ... The final sample was concentrated to 20 mg/ml with a Vivaspin 2 device (2 kDa cutoff, Sartorius).
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bioRxiv - Microbiology 2023Quote: ... Final separation was achieved following concentrating the Heparin elution to approximately 8 mg/mL using a 30 kDa MWCO Vivaspin filter (Sartorius), by applying the sample to a Superdex 200 10/300 GL (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5 x 103 cells/well were plated in FBS-free media in the upper chamber of a 96-well Incucyte ClearView cell migration plate (8 µm pore size; #4582 Sartorius). Cells were incubated at 37°C for 45 minutes to allow cells to settle on the membrane before adding RPMI-1640 media supplemented with 10% FBS to the bottom chamber ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing Ldh-EtfAB were pooled and concentrated via ultrafiltration (Vivaspin 6, 30 kDa Cut-off, Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2023Quote: ... which took place overnight on arrival at 4°C (cold room) using 6 Vivaflow200 filtration units (Sartorius) operating in two parallel series ...
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bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
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bioRxiv - Cancer Biology 2023Quote: ... 25 µl of single-cell suspension was dispensed on ready-to-use plates with an 8-channel electronic Picus® pipette (10 to 300 µl, #735361, Sartorius).
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bioRxiv - Systems Biology 2022Quote: ... and then 6 hours after doxorubicin treatment was moved to an incubator connected to an Incucyte microscope (Sartorius). Brightfield images of wells were taken 6 ...
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bioRxiv - Cell Biology 2022Quote: ... 6 ml of the crude CM was passed through a 300 kDa Vivaspin concentrator (Sartorius AG, Göttingen, Germany) by centrifugation at 3,000 g for ∼15 minutes (or until the retentate was ∼300 µl) ...
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bioRxiv - Microbiology 2020Quote: ... Eluted proteins were concentrated using an ultrafiltration membrane with a 10 kDa molecular weight cutoff (Vivaspin 6, Sartorius). To reconstitute the BAM-DolP complex in vitro ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6.0) using a centrifugal concentrator with a molecular weight cutoff of 10 kDa (Vivaspin 6, VS0602, Sartorius). The presence of the fucosidase (62 kDa ...
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Plant Biology 2019Quote: ... Ascorbic acid was added to the medium through filter sterilization method using membrane filters (0.45 μm, Minisart, Sartorius stedim). In this experiment ...
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bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... Purified V-ATPase in buffer containing LMNG was concentrated to 1.9 mg/mL with a 100 kDa MWCO VivaSpin 6 centrifugal concentrator at 2000 ×g for 15 min (Sartorius), followed by a VivaSpin 500 concentrator at 12,000 ×g for 2 min.
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bioRxiv - Biophysics 2023Quote: ... Fractions containing Ub were concentrated and buffer exchanged into pH 6.8 buffer (see above) using Vivaspin 6 centrifugal concentrators with a molecular weight cutoff (MWCO) of 5000 Da (Sartorius). Purified proteins were frozen at −80°C.
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bioRxiv - Microbiology 2023Quote: ... whole well images were taken at 6 h intervals using an Incucyte Zoom S3 live cell imaging system (Sartorius).
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bioRxiv - Microbiology 2023Quote: ... Total green cell count was measured every 6 h using an Incucyte Zoom S3 live cell imaging system (Sartorius) and normalised for confluency variation ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was then concentrated to the approximate concentration of 10 mg/ml using a Vivaspin 6 3,000 MWCO PES (Sartorius) membrane ultrafiltration tube.
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Bioengineering 2024Quote: ... and arginine (pH 6) to a final concentration of ≥200 mg/mL using the Ambr Crossflow system (Sartorius, Germany).
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bioRxiv - Microbiology 2023Quote: ... 2 mM DTT) using a 5-KD concentrator (Sartorius). The purified samples were aliquoted ...
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bioRxiv - Microbiology 2019Quote: ... 300 mM ammonium acetate pH 7.5 (SpaOC/SpaO/OrgB) or 50 mM ammonium acetate pH 8 (SpaO/SpaOC/OrgB/InvC) using Vivaspin® 500 centrifugal concentrators (Sartorius, Göttingen, Germany). SEC-purified proteins were used for all samples but the SpaO/SpaOC/OrgB complex ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions were checked via SDS-PAGE and the cleanest Chz1-containing fraction was concentrated using a 10 kDa cutoff Vivaspin 6 concentrator (Sartorius) at 3,000 g at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclear suspension was centrifugated for 30 min at 15 000 g and supernatant was concentrated using Vivaspin 6 (10 kDa MWCO) centrifugal concentrator (Sartorius). Aliquots of concentrated nuclear extracts were frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Genetics 2023Quote: ... and cells were imaged live every 6 hours using the IncuCyte® ZOO system for automated live cell analysis (Sartorius). Wound closure was analysed by measuring the spatial cell density in the wound area relative to the spatial cell density outside of the wound expressed as the Relative Wound Density (% ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through was collected and concentrated to 6-10 mg/ml using a 100 kDa molecular weight (MW) cutoff centrifugal concentrator (Sartorius) and loaded onto a Superdex 200 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biophysics 2023Quote: Nanodisc samples were concentrated to ∼200 μM in 280 μL in a Vivaspin-6 concentrator with a 30 kDa MWCO (Sartorius). 20 μL D2O was added and gently mixed into the sample ...
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bioRxiv - Neuroscience 2023Quote: ... Eluent from the first two elution fractions after the elimination of dead volume were concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Eluent from the first two elution fractions after the elimination of dead volume were concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... the fractions obtained by affinity chromatography were pooled and concentrated using conical membrane ultrafiltration tubes (Vivaspin 6 mL 10 kDa MWCO, Sartorius) to 7 mg/mL (2 mL) ...
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bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
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bioRxiv - Molecular Biology 2022Quote: ... were mixed together as well as eluates from samples (Pho92-HTP x 2 reps + Pho92-HTP ime4Δ/Δ x 2 reps) before concentration with Vivacon® 500 filtration cartridges (10 kDa MWCO; Sartorius, Cat# VN01H02) to a final volume of 120 μL ...
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bioRxiv - Biophysics 2021Quote: ... using Vivaspin 2 columns (10 kDa molecular weight cutoff, Sartorius). The resulting ...
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bioRxiv - Microbiology 2021Quote: ... Fractions of IMAC eluate containing Pdr5 were pooled and concentrated using a Vivaspin 6 centrifugal concentrator unit (Sartorius AG, Göttingen, Germany) with a molecular weight cut-off (MWCO ...
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bioRxiv - Microbiology 2023Quote: ... A sample of 6 liters of air per min was collected during the <10 min exposure on the 47 mm gelatin filter (Sartorius, Germany). Post exposure ...
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bioRxiv - Cancer Biology 2021Quote: ... Time-lapse phase-contrast and fluorescent channel images are obtained at 6 hour intervals for up to 24 spheroids for each initial condition using an Incucyte S3 (Sartorius, Goettingen, Germany).
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bioRxiv - Biochemistry 2020Quote: ... The peak fractions containing ANT(9) were pooled and concentrated to 6 mg/ml using a Vivaspin 10 kDa cut-off concentrator (Sartorius AG, Germany).
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).
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bioRxiv - Synthetic Biology 2020Quote: ... filtered through a 10 kDa MWCO spin filter (Vivaspin 2; Sartorius), and quantified by Nanodrop OneC ...
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bioRxiv - Molecular Biology 2021Quote: ... depth filtered supernatants were sterile filtered through a Sartopore 2 (Sartorius) filter unit and stored until further processing at 2-8 °C.
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bioRxiv - Synthetic Biology 2021Quote: ... The required amount of antibody was diluted in PBS (5ml) and applied to a Vivaspin 6 concentrator (Sartorius; VS0601; MWCO: 10kDa; 5ml volume). After centrifugation (4000 x g ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Physiology 2022Quote: ... 2 mM DTT) using a Vivaspin-20 concentrator (catalog no. VS2021, Sartorius). Five buffer exchanges with the storage buffer were performed and purified MBP was then aliquoted and stored at −80°C.
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bioRxiv - Microbiology 2022Quote: ... and further concentrated to ∼100 µL using vivaspin 2 ultrafiltration units (Sartorius). All units had polyether sulphone filters with a molecular weight cut-off of 10 kDa ...
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bioRxiv - Synthetic Biology 2021Quote: ... The required amount of tomato lectin was diluted in PBS (5ml) and applied to a Vivaspin 6 concentrator (Sartorius; VS0601; MWCO: 10kDa; 5ml volume). After centrifugation (4000 x g ...
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bioRxiv - Bioengineering 2019Quote: ... and disrupted with a micro-dismembrator (Sartorius, 2×30 s at 3,000 rpm). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... injection grade and passed through a 0.45 + 0.2 µm Sartopore 2 300 (Sartorius) filter for sterile filtration ...