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Citations for Sartorius :
1 - 50 of 444 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... the volume was adjusted to 5 mL and transferred to a Centrifugal Concentrator (Vivaspin 6; Sartorius). The retentate was adjusted to 120 µL prior to analysis.
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
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bioRxiv - Bioengineering 2024Quote: ... The purified protein from elutions containing the fewest contaminating proteins (typically elution steps 3-5) were concentrated in 1× PBS using a 5 kDa cut-off Vivaspin Centrifugal Concentrator (Sartorius, Oakville, Ontario, Canada). Protein concentrations were determined using the micro-BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM DTT) using a 5-KD concentrator (Sartorius). The purified samples were aliquoted ...
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bioRxiv - Cancer Biology 2021Quote: ... We imaged cells every 6 hours for 4-6 days of treatment using Incucyte Zoom (Sartorius, Michigan, USA). Fluorescence was quantified using four locations in each treated sample by the IncuCyte Zoom 2016B software.
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bioRxiv - Microbiology 2021Quote: ... 3 L of water were pre-filtered through the 5 μm filter (Sartorius). The flow-through was further filtered using the 0.2 μm filter (Sterivex) ...
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bioRxiv - Microbiology 2023Quote: ... After concentration (Vivaspin 6 Concentrator, Sartorius Stedim Biotech) and desalting (PD 10 Desalting Columns ...
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bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM of IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, cat #4440) as well as 1:500 of Nuclight Rapid Red Dye (Sartorius ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 μM Caspase 3/7 dye was included in the media (Sartorius #4704). The number of red nuclei (indicating active caspase 3/7 ...
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bioRxiv - Cancer Biology 2023Quote: ... The UGT2B15 promoter 20 base-pair sequence (5’-TAACTTGATTGATTTTTCCT-3’ for wild type and 5’-TAACTTGGCTGTCTTTTCCT-3’ for mutant) was immobilized to a streptavidin SADH sensor chip (Sartorius) in running buffer (10 mM HEPES pH 6.8 ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with a Vivaspin 6 (10 kDa MWCO) (Sartorius), flash-frozen by liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Neuroscience 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... using the Vivaspin 6 centrifugal concentrator (MWCO 1,000,000 kDa) (Sartorius, Australia), was required for high-quality electron microscopy visualization ...
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). The purified and concentrated protein was delivered to GeneScript-Nanjing to raise the anti-rabbit polyclonal antibody.
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). Concentrations of the purified proteins were measured using BCA protein assay kit (Beyotime ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluted complex was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius), and imidazole was removed from the protein solution by applying the sample to a PD MiniTrap Sample Column (Cytiva ...
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bioRxiv - Microbiology 2019Quote: ... 2 mM MgSO4) and concentration of the sample using Vivaspin6 5 kDa MWCO concentrators (Sartorius).
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bioRxiv - Microbiology 2023Quote: ... 2 L or 5 L scale (Biostat B-plus and B-DCU units, Sartorius Stedium Biotech) as previously described [PMID ...
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bioRxiv - Cell Biology 2021Quote: ... concentrated to 300-350 µl using Vivaspin 6 30,000 MWCO concentrators (Sartorius), and loaded onto a Superdex 200 10/300 GL column equilibrated in SEC Buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80°C before use.
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bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80 °C before use.
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bioRxiv - Biochemistry 2023Quote: ... Dialysed protein was spin-concentrated in a Vivaspin 6 MWCO 50,000 (Sartorius) to the desired concentration ...
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bioRxiv - Systems Biology 2023Quote: ... followed by live-cell imaging every 6 h using Incucyte Systems (Sartorius) for the duration of 1 week ...
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bioRxiv - Biochemistry 2020Quote: ... Two 5 L bioreactors (Sartorius) were seeded at 0.3 x 106 cells/mL in 3 L (total working volume ...
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bioRxiv - Immunology 2020Quote: ... concentrated to 1-5 mg/ml by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at -80°C.
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bioRxiv - Bioengineering 2020Quote: ... GO16 KS3 was cultivated in a 5 L stirred tank bioreactor (Sartorius UniVessel Glass 5 l) containing 3 L of MSMlim broth ...
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bioRxiv - Biochemistry 2020Quote: ... or Vivaspin (Sartorius, MWCO 5 kDa) concentration columns.
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3–5 x103 cells were seeded in 96-well plates and viable cell numbers were determined using the Incucyte (Sartorius) Adherent-Cell-by-Cell module ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsamples were weighed (20 mg ± 6 mg) on a fine scale balance (Entris, Sartorius). Together with 6 negative controls (200 μl lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... the samples were concentrated using filter columns (Vivaspin 6, 100,000 MWCO PES, Sartorius, Germany) at 4 °C with a maximal speed of 4,000 x g and washed twice with DBCM2 salt solution ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Plant Biology 2021Quote: ... and 5-μm syringe filter (Sartorius, Germany) to remove RLX (0 ...
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bioRxiv - Microbiology 2022Quote: ... and BA.2+R493Q RBDs were diluted to 5 μg/mL and immobilized onto pre-hydrated streptavidin biosensors (Sartorius) to a level of 1 nm total shift ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was then concentrated using Vivaspin® 6 100,000 MWCO concentrator columns (Sartorius). The sample was then purified via size exclusion chromatography using a Superose™ 6 Increase column (GE Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Immunology 2022Quote: ... and the eluate was collected into a Vivaspin 6 50 kDa MWCO column (Sartorius, V10631), which was centrifugated at 2,113 g for five minutes to concentrate the antibodies ...
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bioRxiv - Genetics 2023Quote: ... The eluate was washed 3 times with PBS containing 5% Sorbitol and concentrated with Vivaspin 20 100K concentrator (Sartorius Stedim, Bohemia, NY). Vector titer was determined by qPCR with primers and labeled probe targeting the ITR sequence (Aurnhammer et al. ...
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bioRxiv - Biochemistry 2021Quote: ... concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius), and purified via size-exclusion chromatography with a Superdex S75-10/300 column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Brightfield images were taken every 2 hours for 5 days and the percentage confluency calculated using an Incucyte Live-Cell analysis system (Sartorius).