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Citations for Sartorius :
101 - 150 of 435 citations for 6 CHLORO 1 2 4 TRIAZOLO 4 3 B PYRIDAZINE 3 THIOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius). The protein was detected by SDS-PAGE (Supplementary Fig ...
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bioRxiv - Neuroscience 2020Quote: ... the iodixanol was removed from the AAV vector containing fraction by 3 rounds of diafiltration using Vivaspin 20 ultrafiltration devices (100’000 MWCO, PES membrane, Sartorius) and 1x phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Cytiva) in 50 mM sodium acetate (pH 5.2) and concentrated using Vivaspin polyethersulfone membrane (3 kDa cut-off, Sartorius). Protein concentrations were determined using the Bradford method (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... the samples were centrifuged at 17,000 g for 3 min and 150 µl supernatant of each sample was subsequently filtered through a 0.8 µm filter (Sartorius). This filtrate was treated with a mix of Benzonase (50 U ...
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bioRxiv - Microbiology 2019Quote: ... Peak fractions were pooled and concentrated to ∼30 mg/mL using a Vivaspin 4 Turbo concentrator (Sartorius).
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bioRxiv - Microbiology 2020Quote: ... and diplopterol (Chiron AS) were dissolved in acetonitrile and filtered with Minisart RC 4 (Sartorius, Stonehouse, UK) before applying 5 μl aliquots to a Acquity UPLC BEH C18 column (1.7 μm ...
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bioRxiv - Microbiology 2020Quote: ... Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius). The concentrate was then dialyzed using a dialysis tube (Millipore ...
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bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
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bioRxiv - Cancer Biology 2022Quote: ... Four images were acquired per well every 4 hours and analyzed by the IncuCyte 2020B software (Sartorius) to determine cell confluence ...
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bioRxiv - Microbiology 2020Quote: A BIOSTAT® B bioreactor (Sartorius Stedim) with a 1.5-L capacity UniVessel® glass vessel was used for growth of 800-mL cultures at 37°C ...
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bioRxiv - Biochemistry 2019Quote: The flow through from the above column was concentrated to 3 mL using a 10 kDa centrifugal concentrator (Sartorius Stedim) and loaded onto a pre-equilibrated (with buffer D ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We further weighted 3-week old plants with a precision balance (Sartorius AC 210 P with accuracy of 0.1 mg) to quantify variation in plant biomass ...
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bioRxiv - Biochemistry 2020Quote: ... were exchanged into phosphate buffer (pH 7.4) using disposable Vivaspin® 500 centrifugal concentrators with an MWCO of 3 kDa (Sartorius) to remove Cu2+ or H202 ...
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bioRxiv - Biophysics 2021Quote: ... and 0.01 mg/mL 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were added before concentration in Vivaspin® 100,000 kDa molecular weight cut-off (MWCO) centrifugal concentrators (Sartorius) to a volume of ∼500 μL ...
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bioRxiv - Biochemistry 2020Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and Ub/LC3B-PA was concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C.
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were additionally purified through a Superdex 200 26/60 size exclusion column (Pharmacia Biotech) and concentrated using a Vivaspin Turbo 3 kDa concentrator (Sartorius). Protein concentrations were measured using a UV/Vis spectrophotometer (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... S2K146 and respective UCA Ab were prepared at 3 μg/ml and immobilized on pre-hydrated protein-A biosensors (Sartorius) for 75 sec ...
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bioRxiv - Biochemistry 2023Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and the probes were concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C ...
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3–5 x103 cells were seeded in 96-well plates and viable cell numbers were determined using the Incucyte (Sartorius) Adherent-Cell-by-Cell module ...
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Biophysics 2021Quote: ... at pH 7 via centrifugal filter units at 12000 g at 4°C (Vivaspin 500, MWCO 10000, Sartorius). Glycans for native MS analysis were mixed with 1 µM (per monomer ...
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bioRxiv - Biochemistry 2023Quote: ... Obtained EHEP was then concentrated to 15–25 mg/mL using Vivaspin-4 10K columns (Sartorius, Göttingen, Germany). About akuBGL ...
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bioRxiv - Cancer Biology 2023Quote: ... cell confluency was determined every 4 hours using live cell imaging system Incucyte® S3 (Sartorius, Göttingen, Germany) and plotted relative to initial values.
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed solution was finally concentrated by centrifugation through a Vivaspin Turbo 4 membrane filter (MWCO 10,000; Sartorius) at 3,500 × g for 30-45 min at 4°C.
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bioRxiv - Synthetic Biology 2022Quote: ... The seed culture from each flask was transferred to a 1-L bioreactor (Biostat B-DCU, Sartorius, Germany) with 0.7 L fermentation medium ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Cancer Biology 2023Quote: 2,000 cells per well were plated in 96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network external controller hard drive that gathered and processed image data ...
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bioRxiv - Cancer Biology 2023Quote: Analysis of apoptosis using fluorescence live cell microscopy Cells were stained with Incucyte® Caspase-3/7 Dye for Apoptosis (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were used to collect air on 37 mm sterile gelatin filters (3 µm pore size; Sartorius, Cat. No. 12602-37-ALK) containing a backing filter to ensure the gelatin filter remained intact during sampling ...
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bioRxiv - Biochemistry 2020Quote: ... oxidized BdiGolSase1 was ultrafiltrated by centrifuging at 10,000 x g and 4°C using a Vivaspin 500 device (Sartorius). To evaluate the reversibility of the process ...
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bioRxiv - Biochemistry 2021Quote: ... appropriate fractions (selected by 4-20% gradient SDS-PAGE) were pooled and concentrated by centrifugal filtration (Vivaspin 20, 5,000 MWCO PES, Sartorius) for loading onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... 250 mM) at pH 7.5 and 4°C via centrifugal filter units (13000 x g, Vivaspin 500, MWCO 10000 (Sartorius) or Micro Bio-Spin 6 columns (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... were concentrated to 2 ml by centrifugation 30 min at 4,000xg at 4°C through a 20 ml VivaSpin 20 30 kDa MWCO concentrator (Sartorius, and were diluted with TGE containing 1 mM β-mercaptoethanol to adjust the NaCl concentration to 50 mM ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was filtered with Minisart RC4 (pore size 0.20 µm, diameter 4 mm, Sartorius stedim biotech, Göttingen, Germany) and injected into a high-performance liquid chromatography system (LC-10AD ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were thawed and filtrated through a 0.2 µm filter before centrifugation at 4500 x g at 4°C in VivaSpin tubes (Sartorius). The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed once in PBS and kept in 500μL PBS at 4°C in the dark until analysis with an Intellicyt iQue3 Screener (Sartorius). For viability analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Phase contrast images were acquired every 4 hours over a period of 48 hours using an Incucyte machine (Sartorius). The percentage of cell confluence was calculated using the attached Incucyte®software (Sartorius).
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bioRxiv - Biochemistry 2023Quote: ... The resulting protein was concentrated to ≈4 mg/mL using a 5 kDa centrifugal concentrator (Vivaspin; Sartorius, Gottingen, Germany). For drop-deposition Raman spectroscopy ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 3 mL of Glutathione Sepharose 4B resin (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 70,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 4 mL of IgG beads (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). Cleared lysates were then incubated with 1.5 mL of StrepTactin Sepharose beads (IBA Lifesciences ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). For purification of ScaC and ScaC-SNAP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... approximately 4 mg of pulverized tissue was weighed using an ultra-micro balance (CP2P Microbalance, Sartorius GmbH, Goettingen, Germany), packaged into tin capsules ...
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bioRxiv - Genetics 2023Quote: ... The eluate was washed 3 times with PBS containing 5% Sorbitol and concentrated with Vivaspin 20 100K concentrator (Sartorius Stedim, Bohemia, NY). Vector titer was determined by qPCR with primers and labeled probe targeting the ITR sequence (Aurnhammer et al. ...
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bioRxiv - Zoology 2022Quote: ... and 500 µL 10X Amphotericin B (AmB) Solution (Sartorius) for 3 times ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent and phase contrast images are collected at intervals of 4 hours or shorter throughout the study in the Incucyte S2 Live Cell Analysis System (Essen/Sartorius) with temperature and environmental control ...