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Citations for Sartorius :
151 - 200 of 325 citations for 6 4 methylpiperazin 1 yl pyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
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bioRxiv - Cancer Biology 2022Quote: ... Four images were acquired per well every 4 hours and analyzed by the IncuCyte 2020B software (Sartorius) to determine cell confluence ...
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bioRxiv - Biochemistry 2019Quote: The flow through from the above column was concentrated to 3 mL using a 10 kDa centrifugal concentrator (Sartorius Stedim) and loaded onto a pre-equilibrated (with buffer D ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We further weighted 3-week old plants with a precision balance (Sartorius AC 210 P with accuracy of 0.1 mg) to quantify variation in plant biomass ...
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bioRxiv - Biochemistry 2020Quote: ... were exchanged into phosphate buffer (pH 7.4) using disposable Vivaspin® 500 centrifugal concentrators with an MWCO of 3 kDa (Sartorius) to remove Cu2+ or H202 ...
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bioRxiv - Biophysics 2021Quote: ... and 0.01 mg/mL 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were added before concentration in Vivaspin® 100,000 kDa molecular weight cut-off (MWCO) centrifugal concentrators (Sartorius) to a volume of ∼500 μL ...
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bioRxiv - Biochemistry 2020Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and Ub/LC3B-PA was concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C.
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were additionally purified through a Superdex 200 26/60 size exclusion column (Pharmacia Biotech) and concentrated using a Vivaspin Turbo 3 kDa concentrator (Sartorius). Protein concentrations were measured using a UV/Vis spectrophotometer (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... S2K146 and respective UCA Ab were prepared at 3 μg/ml and immobilized on pre-hydrated protein-A biosensors (Sartorius) for 75 sec ...
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bioRxiv - Biochemistry 2023Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and the probes were concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 3–5 x103 cells were seeded in 96-well plates and viable cell numbers were determined using the Incucyte (Sartorius) Adherent-Cell-by-Cell module ...
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bioRxiv - Synthetic Biology 2023Quote: ... Four images per well were acquired at 10x magnification in the green channel (200 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hrs using an Incucyte (Sartorius). A cytotoxicity index was calculated by dividing total green fluorescence intensity per mm2 at every time point by the intensity per mm2 at the first time point ...
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bioRxiv - Synthetic Biology 2023Quote: ... One image per well was acquired at 4x magnification in the green channel (100 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hr using an Incucyte (Sartorius).
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Cell Biology 2023Quote: ... of either parental or mNG-modified cells were plated per well in a 6-well plate in triplicate and placed in an Incucyte system (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Four images per well (total =12 per sample ...
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bioRxiv - Biophysics 2021Quote: ... at pH 7 via centrifugal filter units at 12000 g at 4°C (Vivaspin 500, MWCO 10000, Sartorius). Glycans for native MS analysis were mixed with 1 µM (per monomer ...
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bioRxiv - Cancer Biology 2023Quote: ... cell confluency was determined every 4 hours using live cell imaging system Incucyte® S3 (Sartorius, Göttingen, Germany) and plotted relative to initial values.
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bioRxiv - Biochemistry 2023Quote: ... Obtained EHEP was then concentrated to 15–25 mg/mL using Vivaspin-4 10K columns (Sartorius, Göttingen, Germany). About akuBGL ...
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed solution was finally concentrated by centrifugation through a Vivaspin Turbo 4 membrane filter (MWCO 10,000; Sartorius) at 3,500 × g for 30-45 min at 4°C.
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Immunology 2021Quote: ... 7 L of Sf9 cell culture at a density of 2 × 106 cells/mL were infected with the baculovirus at a MOI of 3 using a Biostat B plus bioreactor (Sartorius, Germany). The following conditions were maintained during the culture period ...
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bioRxiv - Cancer Biology 2023Quote: 2,000 cells per well were plated in 96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network external controller hard drive that gathered and processed image data ...
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bioRxiv - Cancer Biology 2023Quote: Analysis of apoptosis using fluorescence live cell microscopy Cells were stained with Incucyte® Caspase-3/7 Dye for Apoptosis (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were used to collect air on 37 mm sterile gelatin filters (3 µm pore size; Sartorius, Cat. No. 12602-37-ALK) containing a backing filter to ensure the gelatin filter remained intact during sampling ...
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bioRxiv - Biochemistry 2020Quote: ... oxidized BdiGolSase1 was ultrafiltrated by centrifuging at 10,000 x g and 4°C using a Vivaspin 500 device (Sartorius). To evaluate the reversibility of the process ...
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bioRxiv - Biochemistry 2021Quote: ... appropriate fractions (selected by 4-20% gradient SDS-PAGE) were pooled and concentrated by centrifugal filtration (Vivaspin 20, 5,000 MWCO PES, Sartorius) for loading onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... 250 mM) at pH 7.5 and 4°C via centrifugal filter units (13000 x g, Vivaspin 500, MWCO 10000 (Sartorius) or Micro Bio-Spin 6 columns (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... were concentrated to 2 ml by centrifugation 30 min at 4,000xg at 4°C through a 20 ml VivaSpin 20 30 kDa MWCO concentrator (Sartorius, and were diluted with TGE containing 1 mM β-mercaptoethanol to adjust the NaCl concentration to 50 mM ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was filtered with Minisart RC4 (pore size 0.20 µm, diameter 4 mm, Sartorius stedim biotech, Göttingen, Germany) and injected into a high-performance liquid chromatography system (LC-10AD ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were thawed and filtrated through a 0.2 µm filter before centrifugation at 4500 x g at 4°C in VivaSpin tubes (Sartorius). The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting protein was concentrated to ≈4 mg/mL using a 5 kDa centrifugal concentrator (Vivaspin; Sartorius, Gottingen, Germany). For drop-deposition Raman spectroscopy ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed once in PBS and kept in 500μL PBS at 4°C in the dark until analysis with an Intellicyt iQue3 Screener (Sartorius). For viability analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Phase contrast images were acquired every 4 hours over a period of 48 hours using an Incucyte machine (Sartorius). The percentage of cell confluence was calculated using the attached Incucyte®software (Sartorius).
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 3 mL of Glutathione Sepharose 4B resin (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 70,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 4 mL of IgG beads (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). Cleared lysates were then incubated with 1.5 mL of StrepTactin Sepharose beads (IBA Lifesciences ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). For purification of ScaC and ScaC-SNAP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... approximately 4 mg of pulverized tissue was weighed using an ultra-micro balance (CP2P Microbalance, Sartorius GmbH, Goettingen, Germany), packaged into tin capsules ...
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bioRxiv - Immunology 2022Quote: Biotinylated OspCA (3 μg/mL) in buffer (PBS containing 2% w/v BSA) was captured onto streptavidin biosensors (#18-5019, Sartorius, Goettingen, Germany) for 5 min ...
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bioRxiv - Genetics 2023Quote: ... The eluate was washed 3 times with PBS containing 5% Sorbitol and concentrated with Vivaspin 20 100K concentrator (Sartorius Stedim, Bohemia, NY). Vector titer was determined by qPCR with primers and labeled probe targeting the ITR sequence (Aurnhammer et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 1% penicillin/streptomycin (PEN-STREP) and 1% L-glutamine (Sartorius). Cells were incubated at 37C ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent and phase contrast images are collected at intervals of 4 hours or shorter throughout the study in the Incucyte S2 Live Cell Analysis System (Essen/Sartorius) with temperature and environmental control ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were spun at 15,000g for 15 min at 4°C and supernatants were then passed through a regenerated cellulose filter (4mm Ø, Sartorius). Samples were then dried under N2 flow at 40°C and resuspended in 125µl of ice-cold methanol/water 70:30 for subsequent analyses.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The resulting peptide solutions were centrifuged at 13,000 rpm for 15 min at 4 °C on tubes equipped with 2000 MW filtrate tubes (Sartorius, USA). Then ...
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bioRxiv - Biophysics 2020Quote: ... MgCl2 and EGTA were removed by 4 times buffer exchange using a Vivaspin 500.5 molecular weight cut-off (Sartorius, Göttingen). Samples were loaded onto a SDS-PAGE gel to confirm phosphorylation.
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bioRxiv - Cancer Biology 2022Quote: ... Cell proliferation was recorded for up to 7 days every 4 hours by the IncuCyte S3 Live-Cell Analysis System (Sartorius) with a phase-contrast microscope at 10X magnification ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing pure Fab16 were collected and concentrated to approximately 14 mg/ml using a VivaSpin 4 concentrator (10 kDa MWCO, Sartorius). Fab16 was crystallized by vapor diffusion at 4 °C by dispensing 200 nl of protein and 200 nl of crystallization buffer containing 1.5 M malic acid (pH 7.5) ...