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Citations for Sartorius :
451 - 500 of 503 citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: The batch fermentations were performed in BIOSTAT™ Qplus 1-L bioreactors (Sartorius, Göttingen, Germany) with a working culture volume of 800 mL ...
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bioRxiv - Plant Biology 2023Quote: ... and plants were weighed with a BSA224S-CW 1/10,000 analytical balance (Sartorius, Beijing, China). Three biological replicate samples were analyzed for these assays (Chen et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mM EDTA containing Complete® EDTA-free protease inhibitors using a Potter S homogenizer (Sartorius). After addition of 1% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... fresh medium was supplemented with 1:10 000 IncuCyte® Cytotox Green Reagent (Sartorius, Göttingen, Germany) to determine dead cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing A2M tetramers were combined and concentrated to 1 ml final volume (Vivaspin Turbo 15, Sartorius).
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bioRxiv - Cancer Biology 2021Quote: ... HA-Ub75-thioester was concentrated to a concentration of ∼1 mg/ml using 3,000 MW filters (Sartorius) and then desalted against PBS using a PD10 column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... Fermentation was conducted using a Biostat Qplus Bioreactor with a 1 L vessel (Sartorius Stedim, Guxhagen, Germany). For 5 L runs ...
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bioRxiv - Microbiology 2022Quote: A microbial community was enriched in a 1-l bioreactor (BIOSTAT® A plus, Sartorius, Göttingen, Germany), inoculated with broth from a former study [16] and fed with mineral medium containing xylan and lactate over 150 days [13] ...
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bioRxiv - Plant Biology 2023Quote: ... The purest fractions were combined and concentrated to 1 ml using Vivaspin 30K centrifugal concentrators (Sartorius, VS0621). The protein sample was then further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the complex were concentrated to 1 mg/mL using a Vivaspin centrifugal concentrator (Sartorius).
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bioRxiv - Synthetic Biology 2022Quote: ... The seed culture from each flask was transferred to a 1-L bioreactor (Biostat B-DCU, Sartorius, Germany) with 0.7 L fermentation medium ...
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bioRxiv - Biochemistry 2022Quote: ... protein solutions were concentrated to about 12 mg ml-1 using a centricon centrifugal filter unit (Sartorius Stedim). The protein concentration was estimated by measuring the absorbance at 280 nm.
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bioRxiv - Neuroscience 2022Quote: ... was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Microbiology 2023Quote: ... and the wells were rinsed twice with 1× phosphate-buffered saline (PBS, obtained from Sartorius, Beit HaEmek, Israel). This process aimed to remove non-adherent bacterial cells ...
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bioRxiv - Cell Biology 2023Quote: Fractions from the gel filtration peak were concentrated to 1 mg/mL using a Vivaspin® Turbo (Sartorius) centrifugal filter ...
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bioRxiv - Molecular Biology 2023Quote: ... was added at 1:1000 dilution and live cell imaging was performed on the Incucyte S3 System (Sartorius). Apoptosis was calculated using the S3 Live Cell Analysis Instrument (Sartorius ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM TCEP,10% glycerol) and concentrated using Vivaspin turbo with a 10 kDa molecular mass cutoff concentrator (Sartorius) to a final concentration of 1 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... The sera from two healthy donors (1.5 ml) were diluted two-fold and concentrated (to 1 ml final volume) using Vivaspin Turbo 15 (Sartorius) units ...
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bioRxiv - Microbiology 2020Quote: ... Samples (1 mL) from the liquid phase of the cultures were filtered (MiniSart RC4, 0.2 μm, Sartorius, Göttingen, Germany) and acidified with 2.5 μL H2SO4 ...
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bioRxiv - Systems Biology 2022Quote: ... The culture pH was kept at 5.0 by automatic addition of 0.5 mol.l-1 KOH (BioStat controller, type 8843415, Sartorius AG, Germany).
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bioRxiv - Cell Biology 2023Quote: ... samples were denatured in UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and centrifuged on a 30 kDa cut-off filter device (PALL or Sartorius) and washed thrice with UA buffer and twice with 50 mM ammoniumbicarbonate (ABC) ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs were labelled with 1:1000 Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Incucyte (Sartorius) while cells death was measured by adding the IncuCyte Caspase 3/7 Green Apoptosis Assay Reagent 1:5000 to the cocultures (Essen BioScience) ...
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bioRxiv - Microbiology 2023Quote: ... Vesicles were purified from 1 L of culture per replicate using a tangential flow filtration system (Vivaflow 200, Sartorius). Details on all methods ...
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bioRxiv - Molecular Biology 2024Quote: ... PBMCs were labelled with 1:1000 Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Incucyte (Sartorius) while cell death was measured by the IncuCyte Caspase 3/7 Green Apoptosis Assay Reagent as explained before ...
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bioRxiv - Biophysics 2019Quote: ... three successive 1:30 dilutions were performed to remove unbound dye molecules using a 30 kDa MWCO centrifugal concentrator (Sartorius) and buffer containing 50 mM Tris/HCl pH 8.0 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... the purified NT-mini/NT*-mini was concentrated to 1 mg/mL with a Vivaspin Turbo10 kDa MWCO concentrator (Sartorius), flash frozen in liquid nitrogen and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... IncuCyte® Annexin V Green Reagent was added directly in the medium at the dilution 1:200 and B cells were imaged every hour for 3.5 days using IncuCyte® Live-Cell Analysis System (Sartorius).
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bioRxiv - Neuroscience 2022Quote: ... biotinylated VHH was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Bioengineering 2022Quote: ... Eluate from column 1 above 20mAu was loaded into a 1mL prepacked HyperCel STAR AX column (Sartorius AG, Gottingen, Germany). Flow-through material above 20mAu was collected.
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bioRxiv - Immunology 2023Quote: ... two-fold serial dilutions of the biotinylated avi-tagged CH505 TF chSOSIPv4.1 and Man5GlcNAc2-enriched CH505 M5.G458Y chSOSIPv4.1 Env trimers were immobilized on hydrated Streptavidin (SA) Tips (Octet® Sartorius) for 120 seconds ...
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bioRxiv - Genetics 2023Quote: ... 500 cells were seeded in duplicates in 96-well plates and treated with 1 µg/ml dox for 9-10 days and analyzed with Incucyte (Sartorius). Untreated cells served as reference.
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bioRxiv - Systems Biology 2023Quote: ... Cell dry weight was determined by vacuum filtration of 1 mL of samples on pre-weighed 0.45 µm filter membranes (Sartorius Biolab), followed by 15 min microwaving at 325 W and placement in a desiccator for at least 3 days.
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bioRxiv - Microbiology 2023Quote: ... monoclonal antibodies or VHHs were added to transfected cells at a final concentration of 1 µg/ml and GFP expression was monitored hourly over time with an Incucyte Zoom live cell analysis device (Sartorius) and analyzed with the provided Incucyte software.
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bioRxiv - Cancer Biology 2024Quote: ... the media was changed to 90 µL of growth media containing 1:1000 Incucyte Nuclight Rapid Red Dye (Sartorius, 4717) and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium ...
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bioRxiv - Developmental Biology 2020Quote: ... and LSECs were supplemented with 10µg/ml FSA-FITC (prepared as described in (47)) and 1:1000 IncuCyte® NucLight Rapid Red Reagent (cat. 4717, Sartorius). Cells were imaged hourly over a period of 24 hours using the IncuCyte Cell analysis system (Essen Bioscience) ...
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bioRxiv - Biochemistry 2022Quote: ... A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1) was prepared by ligation and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG) at 50 nM concentration for 7 min at 500 rpm ...
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bioRxiv - Cancer Biology 2022Quote: 700 cancer and 1400 stellate cells were seeded in culture medium containing 1% FBS into the apical compartment of an Incucyte Clearview 96-well plate (Sartorius, 4582) at a 1:2 ratio ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The 50 ml culture supernatant containing Strep-tagged CDA proteins was concentrated to 1 ml and exchanged to buffer W (100 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, pH 8) using 10 kD cutoff centrifugal filters (Sartorius, Germany) before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences ...
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bioRxiv - Biochemistry 2024Quote: ... The 35-mer template with a biotin-TEG at the 5’-overhang was annealed to an 11-mer primer (Supplementary Table 1) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). A chimeric RNA-DNA primer demonstrating the highest affinity to Polα (45 ...
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bioRxiv - Microbiology 2019Quote: ... adapted by us to the use of 100 µg protein for digestion with sequencing-grade trypsin in 50:1 ratios in Vivacon 10k filters (Sartorius AG, Germany) (28) ...
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bioRxiv - Genomics 2020Quote: ... Before grinding we added 750 μl Buffer 1 (181 mM Trisodium phosphate, 121 mM guanidinium thiocyanate, 0.22 μM sterile filtered with Sartorius UK Ltd, 16532K) and 60 μl Buffer 2 (150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... filtered at 0.22 μm and concentrated to 1 mL using a Vivaspin turbo ultrafiltration units (10 kDa MWCO, Sartorius AG, Goettingen, Germany). Samples were further concentrated to 50 μL after five successive washes using Tris 50 mM ...
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bioRxiv - Physiology 2019Quote: ... were inoculated with 2 mL of stock culture and grown at 30°C and at 200 rpm for 8 h in a B Braun Certomat BS-1 incubator (Sartorius, Melsungen, Germany). Bioreactor batch cultures were started by transferring 400 mL of preculture to a bioreactor containing 4.6 L of medium ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cell dry weight (CDW) measurements were performed by filtrating 1 mL of cell culture through a weighed 0.45 μm filter membrane (Sartorius Biolab, Gottingen, Germany) and weighting the filter after microwaving it for 15 min and letting it dry in a desiccator for 24h.
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bioRxiv - Plant Biology 2023Quote: The biolayer interferometry was performed as previously described (Xie et al., 2022) using Octet® R2 Protein Analysis System (Sartorius, Figure 1) or Gator-plus (Gator Bio ...
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bioRxiv - Cell Biology 2023Quote: ... HoxB8 cells were seeded in media with or without 1 µg/ml Doxycycline and 1 µg/ml PI and imaged every 2h in an Incucyte S3 microscope using the 10x objective (Sartorius, Göttingen, Germany). The analysis function of the system was used to calculate the red object count (ROC) ...
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bioRxiv - Immunology 2023Quote: All BLI for data shown in Figure 1 and Figure S1 was carried out on an OctetRED384 (ForteBio) using anti-human Fc-capture sensors (Sartorius, 18-5060) to immobilize anti-PfRH5 mAbs ...
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bioRxiv - Genetics 2021Quote: ... according to manufacturer’s protocol and cultured at 1×105 cells/mL in CellGenix GMP SCGM serum-free base media (Sartorius CellGenix GmbH, Freiburg, Germany) supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Genomics 2022Quote: ... according to manufacturer’s protocol and cultured at 1×105 cells/mL in CellGenix GMP SCGM serum-free base media (Sartorius CellGenix GmbH, Freiburg, Germany) supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Bioengineering 2021Quote: ... The plate was centrifuged at 100g for 1 minute and then placed in The IncuCyte® S3 Live-Cell Analysis System (Sartorius, Catalog No 4647), stored at 37°C ...