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Citations for Sartorius :
251 - 300 of 430 citations for 6' CHLORO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Protein was concentrated from the media using VivaSpin Turbo 4 (10000 MWCO) concentrator tubes (Sartorius, VS04T01). Protein samples were run on 4-15% Mini protean TGX stain-free gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... every 5 min for up to 20 h or until a quasi-equilibrium was reached using a micro-balance (Sartorius MSE3.6P-000-DM, readability 0.0010 mg). The chamber of the micro-balance was half-opened to keep air circulating with the environmental chamber ...
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bioRxiv - Biophysics 2019Quote: ... three successive 1:30 dilutions were performed to remove unbound dye molecules using a 30 kDa MWCO centrifugal concentrator (Sartorius) and buffer containing 50 mM Tris/HCl pH 8.0 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... the purified NT-mini/NT*-mini was concentrated to 1 mg/mL with a Vivaspin Turbo10 kDa MWCO concentrator (Sartorius), flash frozen in liquid nitrogen and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... IncuCyte® Annexin V Green Reagent was added directly in the medium at the dilution 1:200 and B cells were imaged every hour for 3.5 days using IncuCyte® Live-Cell Analysis System (Sartorius).
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bioRxiv - Neuroscience 2022Quote: ... biotinylated VHH was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Bioengineering 2022Quote: ... Eluate from column 1 above 20mAu was loaded into a 1mL prepacked HyperCel STAR AX column (Sartorius AG, Gottingen, Germany). Flow-through material above 20mAu was collected.
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bioRxiv - Immunology 2023Quote: ... two-fold serial dilutions of the biotinylated avi-tagged CH505 TF chSOSIPv4.1 and Man5GlcNAc2-enriched CH505 M5.G458Y chSOSIPv4.1 Env trimers were immobilized on hydrated Streptavidin (SA) Tips (Octet® Sartorius) for 120 seconds ...
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bioRxiv - Microbiology 2023Quote: ... monoclonal antibodies or VHHs were added to transfected cells at a final concentration of 1 µg/ml and GFP expression was monitored hourly over time with an Incucyte Zoom live cell analysis device (Sartorius) and analyzed with the provided Incucyte software.
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bioRxiv - Genetics 2023Quote: ... 500 cells were seeded in duplicates in 96-well plates and treated with 1 µg/ml dox for 9-10 days and analyzed with Incucyte (Sartorius). Untreated cells served as reference.
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bioRxiv - Systems Biology 2023Quote: ... Cell dry weight was determined by vacuum filtration of 1 mL of samples on pre-weighed 0.45 µm filter membranes (Sartorius Biolab), followed by 15 min microwaving at 325 W and placement in a desiccator for at least 3 days.
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bioRxiv - Cancer Biology 2024Quote: ... the media was changed to 90 µL of growth media containing 1:1000 Incucyte Nuclight Rapid Red Dye (Sartorius, 4717) and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium ...
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bioRxiv - Microbiology 2019Quote: ... and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius). The protein was detected by SDS-PAGE (Supplementary Fig ...
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bioRxiv - Neuroscience 2020Quote: ... the iodixanol was removed from the AAV vector containing fraction by 3 rounds of diafiltration using Vivaspin 20 ultrafiltration devices (100’000 MWCO, PES membrane, Sartorius) and 1x phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Cytiva) in 50 mM sodium acetate (pH 5.2) and concentrated using Vivaspin polyethersulfone membrane (3 kDa cut-off, Sartorius). Protein concentrations were determined using the Bradford method (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... the samples were centrifuged at 17,000 g for 3 min and 150 µl supernatant of each sample was subsequently filtered through a 0.8 µm filter (Sartorius). This filtrate was treated with a mix of Benzonase (50 U ...
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bioRxiv - Bioengineering 2019Quote: ... and disrupted with a micro-dismembrator (Sartorius, 2×30 s at 3,000 rpm). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... injection grade and passed through a 0.45 + 0.2 µm Sartopore 2 300 (Sartorius) filter for sterile filtration ...
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bioRxiv - Molecular Biology 2021Quote: ... Buffer exchanged supernatants were filtered using 0.8 + 0.45 µm Sartopore 2 filters (Sartorius) particulate contaminant removal and further sterile filtered using 0.2 µm Sartopore 2 filters (Sartorius) ...
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bioRxiv - Neuroscience 2022Quote: ... then plates were imaged every 2 hours using an Incucyte Zoom system (Sartorius) to quantify the number of fluorescent cells per field of view.
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bioRxiv - Developmental Biology 2020Quote: ... and LSECs were supplemented with 10µg/ml FSA-FITC (prepared as described in (47)) and 1:1000 IncuCyte® NucLight Rapid Red Reagent (cat. 4717, Sartorius). Cells were imaged hourly over a period of 24 hours using the IncuCyte Cell analysis system (Essen Bioscience) ...
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bioRxiv - Biochemistry 2022Quote: ... A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1) was prepared by ligation and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG) at 50 nM concentration for 7 min at 500 rpm ...
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bioRxiv - Cancer Biology 2022Quote: 700 cancer and 1400 stellate cells were seeded in culture medium containing 1% FBS into the apical compartment of an Incucyte Clearview 96-well plate (Sartorius, 4582) at a 1:2 ratio ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The 50 ml culture supernatant containing Strep-tagged CDA proteins was concentrated to 1 ml and exchanged to buffer W (100 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, pH 8) using 10 kD cutoff centrifugal filters (Sartorius, Germany) before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences ...
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Immunology 2023Quote: ... the mNeonGreen signals from infected MRC-5 cells were monitored for 1 week using the Incucyte™ Live-Cell analysis system (Sartorius). Additionally ...
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bioRxiv - Biochemistry 2024Quote: ... The 35-mer template with a biotin-TEG at the 5’-overhang was annealed to an 11-mer primer (Supplementary Table 1) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). A chimeric RNA-DNA primer demonstrating the highest affinity to Polα (45 ...
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bioRxiv - Microbiology 2019Quote: ... Peak fractions were pooled and concentrated to ∼30 mg/mL using a Vivaspin 4 Turbo concentrator (Sartorius).
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bioRxiv - Microbiology 2020Quote: ... and diplopterol (Chiron AS) were dissolved in acetonitrile and filtered with Minisart RC 4 (Sartorius, Stonehouse, UK) before applying 5 μl aliquots to a Acquity UPLC BEH C18 column (1.7 μm ...
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bioRxiv - Microbiology 2020Quote: ... Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius). The concentrate was then dialyzed using a dialysis tube (Millipore ...
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bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
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bioRxiv - Cancer Biology 2022Quote: ... Four images were acquired per well every 4 hours and analyzed by the IncuCyte 2020B software (Sartorius) to determine cell confluence ...
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bioRxiv - Biochemistry 2019Quote: The flow through from the above column was concentrated to 3 mL using a 10 kDa centrifugal concentrator (Sartorius Stedim) and loaded onto a pre-equilibrated (with buffer D ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We further weighted 3-week old plants with a precision balance (Sartorius AC 210 P with accuracy of 0.1 mg) to quantify variation in plant biomass ...
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bioRxiv - Biochemistry 2020Quote: ... were exchanged into phosphate buffer (pH 7.4) using disposable Vivaspin® 500 centrifugal concentrators with an MWCO of 3 kDa (Sartorius) to remove Cu2+ or H202 ...
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bioRxiv - Biophysics 2021Quote: ... and 0.01 mg/mL 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were added before concentration in Vivaspin® 100,000 kDa molecular weight cut-off (MWCO) centrifugal concentrators (Sartorius) to a volume of ∼500 μL ...
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bioRxiv - Biochemistry 2020Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and Ub/LC3B-PA was concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C.
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were additionally purified through a Superdex 200 26/60 size exclusion column (Pharmacia Biotech) and concentrated using a Vivaspin Turbo 3 kDa concentrator (Sartorius). Protein concentrations were measured using a UV/Vis spectrophotometer (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... S2K146 and respective UCA Ab were prepared at 3 μg/ml and immobilized on pre-hydrated protein-A biosensors (Sartorius) for 75 sec ...
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bioRxiv - Biochemistry 2023Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and the probes were concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C ...
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3–5 x103 cells were seeded in 96-well plates and viable cell numbers were determined using the Incucyte (Sartorius) Adherent-Cell-by-Cell module ...
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bioRxiv - Cell Biology 2023Quote: ... of either parental or mNG-modified cells were plated per well in a 6-well plate in triplicate and placed in an Incucyte system (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Four images per well (total =12 per sample ...
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bioRxiv - Microbiology 2019Quote: ... adapted by us to the use of 100 µg protein for digestion with sequencing-grade trypsin in 50:1 ratios in Vivacon 10k filters (Sartorius AG, Germany) (28) ...
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bioRxiv - Immunology 2019Quote: ... Worm culture supernatants collected over 1 week were further concentrated using centrifugal protein concentrators with a 5 kDa MWCO (Vivaspin, Sartorius, Göttingen, Germany) to obtain the final ...
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bioRxiv - Genomics 2020Quote: ... Before grinding we added 750 μl Buffer 1 (181 mM Trisodium phosphate, 121 mM guanidinium thiocyanate, 0.22 μM sterile filtered with Sartorius UK Ltd, 16532K) and 60 μl Buffer 2 (150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... filtered at 0.22 μm and concentrated to 1 mL using a Vivaspin turbo ultrafiltration units (10 kDa MWCO, Sartorius AG, Goettingen, Germany). Samples were further concentrated to 50 μL after five successive washes using Tris 50 mM ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cell dry weight (CDW) measurements were performed by filtrating 1 mL of cell culture through a weighed 0.45 μm filter membrane (Sartorius Biolab, Gottingen, Germany) and weighting the filter after microwaving it for 15 min and letting it dry in a desiccator for 24h.
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bioRxiv - Plant Biology 2023Quote: The biolayer interferometry was performed as previously described (Xie et al., 2022) using Octet® R2 Protein Analysis System (Sartorius, Figure 1) or Gator-plus (Gator Bio ...