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Citations for Sartorius :
51 - 100 of 104 citations for 2 Aminonaphthalene Ring D7 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Triton X-100 and incubation at RT for 60-120 mins and solutions sterile filtered using 0.45 µm Sartopore 2 filters (Sartorius). Triton X-100 inactivated solutions were batch-wise loaded onto a MAb PGT145 immunoaffinity column for capture of ConM and ConS Env trimers ...
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bioRxiv - Biophysics 2021Quote: ... Following sonication, DNA impurities were digested (DNase: Fisher Optizyme, 2 U/ml) and the supernatant was filtered (0.45 μm, Sartorius) and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: Protein-containing fractions were combined and concentrated to 0.5 ml using a 50-kDa molecular weight cutoff (MWCO) Vivaspin 2 concentrator (Sartorius), and desalted by buffer exchange on a PD MiniTrap G-25 column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... and BA.2+R493Q RBDs were diluted to 5 μg/mL and immobilized onto pre-hydrated streptavidin biosensors (Sartorius) to a level of 1 nm total shift ...
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bioRxiv - Biochemistry 2022Quote: ... samples were further concentrated to ~1-2 mg/mL with a 100 kDa MWCO Vivaspin 500 centrifugal concentrator (Sartorius) at 12000 ×g ...
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bioRxiv - Systems Biology 2023Quote: ... The wells were imaged every 2 hours for 120 hours and cell confluence was measured with Incucyte ZOOM (Sartorius).
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bioRxiv - Bioengineering 2022Quote: Fed-batch bioreactor experiments were performed in 2-L bench top glass fermenters (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated EVs were subsequently concentrated to a volume of 100 ul using a 2 ml 100,000 kDa Vivaspin column (Sartorius).
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell monolayer was scraped and migration was monitored via time-lapse image acquisition every 2 hours via the IncuCyte ZOOM imaging platform (Sartorius). Migration rates were calculated as relative wound density over time for wildtype and mutant cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... Brightfield images were taken every 2 hours for 5 days and the percentage confluency calculated using an Incucyte Live-Cell analysis system (Sartorius).
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... Fragmented gDNA was obtained by beat-beating the intact gDNA for 12 minutes using 2 mm diameter glass beads in a Mikro-Dismembrator U (Sartorius) at 2000 rpm ...
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Immunology 2020Quote: ... The EV pellet was resuspended in 2 ml PBS and stored at −80 prior to further concentration using a 5 kDa MW cut-off vivaspin (Sartorius). The protein content of EVs was quantified using Qubit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Images and confluence data were acquired every 2 h for ~200 h using the IncuCyte Live Cell Analysis Imaging System (Sartorius). The Area Under Curve (AUC ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Cell Biology 2023Quote: Isolated ECs were seeded onto 96 well-plates pre-coated with fibronectin in EGM-2 and the proliferation assay was performed for 60 hours in the Incucyte proliferation assay according to (21) (Sartorius). The analysis of the cell proliferation was performed by the Incucyte Basic Analyzer software.
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bioRxiv - Bioengineering 2022Quote: ... Four images were recorded per well every 2 h and analyzed using IncuCyte cell by cell analysis software module (Sartorius). The killing potency of the CAR-T cells was assessed by comparing the percentage of Annexin V positive target cells over time relative to the total number of target cells.
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bioRxiv - Bioengineering 2023Quote: ... This experiment was carried out in a commercial stirred tank bioreactor (STBR) of 2 l total volume (Sartorius AG, Germany) and a working volume of 1 l was used ...
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bioRxiv - Molecular Biology 2023Quote: ... The monodisperse peak of the complex was collected and concentrated with a 50 kDa cut-off concentrator (Vivaspin 2, Sartorius) and subsequently used for crystallization in LCP.
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Cell Biology 2023Quote: ... The remaining vesicle pellets were resuspended in 2 mL sterile 1× PBS and sterile filtered with 0.20 µm syringe filters (Carl Roth, Sartorius VS0271).
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bioRxiv - Immunology 2024Quote: ... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Four images per well were acquired at 10x magnification in the green channel (200 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hrs using an Incucyte (Sartorius). A cytotoxicity index was calculated by dividing total green fluorescence intensity per mm2 at every time point by the intensity per mm2 at the first time point ...
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bioRxiv - Synthetic Biology 2023Quote: ... One image per well was acquired at 4x magnification in the green channel (100 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hr using an Incucyte (Sartorius).
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bioRxiv - Physiology 2024Quote: ... Cultures were monitored for colony formation from 2 to 8 weeks after plating using Incucyte live cell imaging system (Sartorius). Wells with single colonies were harvested and cell cultures were expanded for making stocks and for DNA isolation ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). Dose-response neutralization curves were analyzed by non-linear regression with a variable slope (GraphPadPrism v8 ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Microbiology 2020Quote: ... 1% (w/v) polyvinylpyrrolidone and 2% (w/v) sodium dodecyl sulfate after which 200 mg glass beads (0.11 mm Sartorius, Gottingen, Germany) were added and the cells were lysed for 5 min at 2000 rpm in a FastPrep VR-96 instrument (MP Biomedicals ...
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bioRxiv - Biochemistry 2022Quote: ... A template with a biotin-TEG at the 5’-end was annealed to the primers (Table 2) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). Primers were added at two-fold molar excess over the template ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Immunology 2020Quote: ... and S309 for binding to soluble SARS-CoV-2 S trimer was assessed using the ForteBio Octet HTX (Sartorius Bioanalytical Instruments). All reagents were diluted to 100 nM in PBSF ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 10 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identificationbioRxiv - Cell Biology 2019Quote: ... 50 µl media with 2 x compound concentration was added and plates were subsequently placed and monitored in an IncuCyte® (Sartorius) instrument ...
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bioRxiv - Microbiology 2020Quote: Batch and fed-batch bioreactor experiments were performed in 2 L bench top glass fermentors (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). For experiments using single dilutions of serum or ExpiCHO-S cell supernatant ...
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bioRxiv - Plant Biology 2023Quote: ... 250 mM imidazole) and concentrated to 1-2 mL by ultrafiltration using a Vivaspin® Turbo Centrifugal Concentrator (Sartorius, United Kingdom). To remove imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... as well as phase) were taken every 2 h over a period of 72 h using the IncuCyte® (Sartorius, Germany). General macroautophagy flux was calculated via the ratio of the total integrated fluorescence intensities of GFP/RFP ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed with 2% paraformaldehyde and % GFP positive cells were determined using flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG). Infection was normalized to conditions without antibody ...
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bioRxiv - Plant Biology 2024Quote: ... Fractions were pooled and concentrated to a final concentration of 2 mg/ml using a centrifugal filter device (Sartorius, Göttingen, Germany) with a molecular weight cut-off of 50 kDa.
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bioRxiv - Microbiology 2020Quote: Batch cultivations were performed in biological replicates (between 2-7 per condition) in 1.0 L Biostat Qplus bioreactors (Sartorius Stedim Biotech, Germany). The temperature was controlled at 28°C and pH was maintained at 6.0 (when desired ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Microbiology 2019Quote: The experimental platform is an improved version of a previous on-line flow cytometry platform [28][12] and actually comprises three modules (Figure 1): (a) a conventional culture device (Biostat B-Twin, Sartorius, 2 L), a physical interface for sampling and dilution comprising peristaltic pumps and a mixing chamber (c ...
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bioRxiv - Microbiology 2020Quote: ... Obtained fractions were analyzed by SDS PAGE in reducing conditions and fractions containing proKLK13 or proKLK14 were concentrated with Vivaspin® 2 (Sartorius, Germany) and further purified using size exclusion chromatography (Superdex s75 pg ...