Labshake search
Citations for Sartorius :
401 - 447 of 447 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
-
bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
-
bioRxiv - Microbiology 2023Quote: High cell density fed-batch fermentation for production of Nanobody molecules was performed at 0.25 L (Ambr250 MO units, Sartorius Stedium Biotech), 2 L or 5 L scale (Biostat B-plus and B-DCU units ...
-
bioRxiv - Microbiology 2022Quote: ... Fusion events were visualized for 24 h at 20 min intervals using the IncuCyte® S3 Live-Cell Analysis System (Sartorius). Phase contrast and GFP images (4 per well ...
-
bioRxiv - Bioengineering 2023Quote: ... COS7 cells at a concentration of 60,000 cells/ml in Dulbecco’s Modified Eagle Medium (DMEM) with 1g/l D-glucose (Sartorius, 01-050-1A), supplemented with fetal bovine serum (Biological Industries ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
-
bioRxiv - Immunology 2023Quote: ... cell culture supernatants were harvested and clarified by centrifugation (3000xg for 20 minutes) or using Sartoclear Dynamics® Lab V (Sartorius). Supernatants were then filtered at 0.2 μm using Nalgene Rapid Flow sterile single using vacuum filter units (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... For the Incucyte assay, THP-1 or Jeko-1 cells were transduced with IncuCyte NucLight Red Lentivirus Reagent (EF-1 Alpha, Puro) (Sartorius, USA). All cell lines were repeatedly tested for the presence of mycoplasma contamination with a MycoReport Mycoplasma Detection Kit (Evrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 mL Tris buffer (50mM, pH 7.6) and afterwards lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... Phase confluence cell images were obtained using a 10× objective lens every 6 hours for 168 hours and the average confluence of each well was calculated with the IncuCyte S3 Live Cell Analysis System (Sartorius, USA).
-
bioRxiv - Cell Biology 2024Quote: ... One hundred microliters of the combined LIVE/DEAD® assay reagent was added to each well and incubated for 45 min before quantification of percentage of red and green cells using the IncuCyte® ZOOM (Sartorius) system and its proprietary ZOOM 2016A software.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Green fluorescence was immediately followed every 90 or 120 minutes with an Incucyte® S3 Live-Cell Analysis system (Sartorius, Germany). Images were analyzed with Incucyte software ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...
-
bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
-
bioRxiv - Cancer Biology 2024Quote: The growth and viability of PDAC organoids and PBMCs organoid co-cultures were monitored with the live-cell imaging system IncucyteR S3 (Sartorius, Germany). The Incucyte S3 system from Sartorius proves to be a valuable asset for observing the time-lapse behavior of organoids in response to a particular treatment ...
-
bioRxiv - Cancer Biology 2024Quote: ... and only spheres ≥50 µm in diameter were counted using the IncuCyte® SX1 live cell imaging system (Sartorius, Göttingen, Germany). Frequencies of sphere-forming cells in the experimental groups were calculated and compared using ELDA software [18].
-
bioRxiv - Microbiology 2021Quote: ... The cell-free viral fraction was concentrated to about 50 mL with tangential flow filtration (50R VivaFlow, Sartorius Lab instruments, Goettingen, Germany) and used as inoculum in a multi-step viral enrichment experiment followed by Dilution-to-Extinction purification as described previously (15) ...
-
bioRxiv - Bioengineering 2020Quote: ... 35 mm dishes were imaged using a phase-contrast live-cell imaging system and a 4x objective (IncuCyte Zoom, Sartorius, Gottingen, Germany).
-
bioRxiv - Cancer Biology 2021Quote: ... 4T1 cells with nuclear-localized GFP (4T1-nucGFP) were established by introducing NucLight Green Lentivirus Reagent (Essen BioScience by Sartorius, Goettingen, Germany) under the selection of 2 µg/ml puromycin ...
-
bioRxiv - Biochemistry 2020Quote: ... and incubated at 37°C with 5% CO2 during 24h in the IncuCyte S3 live-cell imaging and analysis system (Essen BioScience, Sartorius, Göttingen, Germany). Images were acquired using a 10X objective and 440-480 nm Excitation / 504-544 nm Emission filters ...
-
bioRxiv - Cancer Biology 2022Quote: ... Confluence was assessed over time using an IncuCyte® Zoom Live Cell Imaging instrument with data analyzed using IncuCyte® Analysis Software (Sartorius) in two-hour intervals.
-
bioRxiv - Synthetic Biology 2022Quote: ... Cell dry weight (CDW) measurements were performed by filtrating 1 mL of cell culture through a weighed 0.45 μm filter membrane (Sartorius Biolab, Gottingen, Germany) and weighting the filter after microwaving it for 15 min and letting it dry in a desiccator for 24h.
-
bioRxiv - Microbiology 2022Quote: ... RKO cells were then incubated for 72 hrs at 37°C in an IncuCyte S3 live imaging incubator (Sartorius and Essun BioScience). Each well was imaged in five locations with a 10X objective every two hrs for a total of 72 hrs (Camera ...
-
bioRxiv - Cancer Biology 2022Quote: ... The percentage of confluent cells was monitored for 125 hours using a high-definition automated imaging system (IncuCyte®; Sartorius, Goettingen, Germany). Data were evaluated using ANOVA and presented as mean ± standard errors ...
-
bioRxiv - Biochemistry 2023Quote: The perfusion cell culture experiments were conducted in a 5 L glass bioreactor using a Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 2 L ...
-
bioRxiv - Immunology 2023Quote: ... Red nuclei as a measurement for the total cell number and IE1 positive nuclei were counted with the IncuCyte S3 (Essen BioSciences, Sartorius, Göttingen, Germany) and the ratio of infected cells was calculated.
-
bioRxiv - Bioengineering 2023Quote: Real-time monitoring of PEO4 cell growth within the hydrogel multispheroids and in the absence of treatment was conducted using an IncuCyte S3 Live Cell Analysis System (Sartorius, Gottingen, Germany). Three cell densities (2000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were stained with a chimeric lectin-library as previously described (38) and screened on IQue Screener Plus (IntelliCyt/Sartorius, Göttingen, DE). All data was analyzed by FlowJo (BD ...
-
bioRxiv - Immunology 2023Quote: ... CD14+-cells were immunomagnetically isolated from PBMCs and incubated with fluorescently labelleled Staphyloccocus aureus and Pseudomonas aeruginosa bioparticles in an Incucyte SX1 life cell imagining incubator (Sartorius, Göttingen, Germany) over 48 hours ...
-
bioRxiv - Bioengineering 2024Quote: ... The plate was centrifuged for 5 minutes at 100g and then placed in the IncuCyte S3 Live-Cell Analysis System (Sartorius, G_ttingen, Germany) and stored at 37°C ...
-
bioRxiv - Genetics 2021Quote: ... according to manufacturer’s protocol and cultured at 1×105 cells/mL in CellGenix GMP SCGM serum-free base media (Sartorius CellGenix GmbH, Freiburg, Germany) supplemented with stem cell factor (SCF)(100ng/mL) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 150 transparent (NTC-sgRNA) MCF7-dcas-9KRAB cells were seeded per well in a 96 wells ImageLock plate (Sartorius – cat no 4379) both in the presence and absence of oestradiol (Complete medium with 10% FCS +/- 17-ß Oestradiol 1x10-8 M (Sigma Aldrich – cat no E-060) ...
-
bioRxiv - Bioengineering 2022Quote: ... and HT29 cells were transfected to express the nuclear-restricted mKate2 red fluorescent protein using the IncuCyte® NucLight Lentivirus Reagents (Sartorius, Cat: 4476) and following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... according to manufacturer’s protocol and cultured at 1×105 cells/mL in CellGenix GMP SCGM serum-free base media (Sartorius CellGenix GmbH, Freiburg, Germany) supplemented with stem cell factor (SCF)(100ng/mL) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was measured over the course of 72hr in the IncuCyte Live Cell Imaging System and data was analyzed using IncuCyte S3 imaging software (Sartorius Ann Arbor, MI).
-
bioRxiv - Cancer Biology 2020Quote: ... Proliferation kinetics as well tumor spheres were recorded using Celigo® S imaging cytometer (Nexcelom Bioscience LLC) and IncuCyte® Live Cell Analysis System (Sartorius AG). Colonies and migrated cells from trans-well assay were washed with PBS ...
-
bioRxiv - Immunology 2022Quote: ... or monitored in short time periods using a 10 x magnification in an Incucyte®S3 Live-Cell Analysis System (Sartorius, Göttingen, Germany). Image analysis was conducted with the machine learning software ilastik [80] using pixel and object classification to distinguish background from cell nuclei and to determine IRF3-GFP and H2B-mCherry colocalization ...
-
bioRxiv - Cancer Biology 2023Quote: Real-time calcein intensity was monitored using fluorescence live-cell imaging combined with a phase-contrast setup using the 4×objective (Sartorius AG, Gottingen, Germany). Images were acquired hourly and analyzed using Incucyte software and ImageJ.
-
bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation for 40 minutes at 3000 g and the phage-containing supernatant was filtered twice (Sartorius MinisartTM 0.2 µm) to obtain a phage stock ...
-
bioRxiv - Biophysics 2024Quote: Cellular morphology and MMP was assessed in real-time using the Incucyte® S3 Live-Cell Analysis System (Sartorius, Ann Arbor, MI, USA). Cells were subjected to SL for 1 hour with the presence of 20 nM TMRM ...
-
bioRxiv - Bioengineering 2021Quote: ... The plate was centrifuged at 100g for 1 minute and then placed in The IncuCyte® S3 Live-Cell Analysis System (Sartorius, Catalog No 4647), stored at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... monocytes were resuspended in PBS and labelled with Incucyte Cytolight rapid green or red reagent for live cell cytoplasmic labelling (0.750 µM, Sartorius, Cat # 4705 and Cat # 4706) for 20 min at 37°C ...
-
bioRxiv - Systems Biology 2023Quote: ... Cells were washed once with warm (37°C) PBS and then lifted from wells using Trypsin EDTA solution B (Sartorius, Cat# 03-052-1B) (5mins ...
-
bioRxiv - Cancer Biology 2021Quote: ... ICG powder was weighed and dissolved in cell culture medium (type of medium dependent on experiment) and sterile filtered (Sartorius Minisart High Flow, PES 0.22μm) to achieve a 100μM stock solution ...
-
bioRxiv - Cell Biology 2023Quote: ... of either parental or mNG-modified cells were plated per well in a 6-well plate in triplicate and placed in an Incucyte system (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Four images per well (total =12 per sample ...
-
bioRxiv - Cancer Biology 2023Quote: ... SK-N-MC and ES5838 (4 x 104) cells per well were plated in a 24-well plate and placed in an IncucyteS3 incubator (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Nine images per well were taken every 6 hrs for seven days ...