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Citations for Sartorius :
351 - 400 of 433 citations for Rat Activated Leukocyte Cell Adhesion Molecule CD166 ALCAM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... were imaged for fluorescent pHrodo-labelled tau protein (excitation at 577nm and emission at 641nm in an acidic environment) using a 20× IncuCyte S3 Live-cell Analysis System (Sartorius). Bright-field and fluorescence emission images were collected at 45min or 1hr intervals for 16hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ and CD8+ T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were cultured in RPMI 1640 medium (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP-NANOG MDA-MB-231 cells were plated on fibronectin-coated (30 μg/mL) glass 96 well plates and placed in an Incucyte S3 (Sartorius) live cell imaging system ...
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bioRxiv - Cancer Biology 2019Quote: TNBC control and gene knocked down cells (500/well) were seeded in 384 well plate and transferred to Incucyte ZOOM analysis system (Sartorius) that was maintained at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Images and confluence data were acquired every 2 h for ~200 h using the IncuCyte Live Cell Analysis Imaging System (Sartorius). The Area Under Curve (AUC ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were grown on a 96-well plate and placed in the incubated chamber of an Incucyte ZOOM Microscope (Sartorius). EpiLC differentiation medium was added and supplemented with 5µM of Caspase 3/7 Green dye (Sartorius ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 104 cells were seeded in each well of an Incucyte® ImageLock 96-well plate (Sartorius, Catalog #: 4379) in 100 μL/well of either complete RPMI or DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... A wound was made in the cell monolayer using a 96-well wound-maker tool with PTFE pin tips (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The fixative was washed off with flow cytometry buffer and cells resuspended in 25 μL of that buffer and analyzed on an iQue Screener PLUS cytometer (Sartorius).
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bioRxiv - Immunology 2022Quote: ... complete media was then layered on top for imaging with an Incucyte S3 live-cell analysis system (Sartorius, Göttingen, Germany). Imaging was performed every hour at 4X magnification using red ...
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Synthetic Biology 2023Quote: ... The preculture cells were then inoculated at initial OD600= 0.1 in triplicates in 1L Biostat Qplus bioreactors (Sartorius Stedim Biotech). The bioreactor cultivations were performed in 500 mL of SDG medium containing 20 g/L galactose (separately autoclaved) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were imaged every 12 h and cell growth and green fluorescence metrics were extracted and analyzed using the IncuCyte ZOOM software (Sartorius).
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bioRxiv - Biochemistry 2022Quote: The bioreactor cell culture experiments were conducted in a 3L glass bioreactor using Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 1.75L ...
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bioRxiv - Immunology 2023Quote: HeLa and MDA-MB-231 WT and ARPC5-KO cells were seeded in a 96-well ImageLock plate (Sartorius #4806) and incubated for 24 h ...
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bioRxiv - Microbiology 2023Quote: ... monoclonal antibodies or VHHs were added to transfected cells at a final concentration of 1 µg/ml and GFP expression was monitored hourly over time with an Incucyte Zoom live cell analysis device (Sartorius) and analyzed with the provided Incucyte software.
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Microbiology 2023Quote: ... Plates of infected macrophages were imaged in triplicate per well in an Incucyte S3 Live-Cell Analysis Platform (Sartorius #4647). Phase and fluorescence images were collected every hour per well using a 20X objective ...
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... before percent of GFP-positive cells was determined by high-throughput flow cytometry (Intellicyt iQue Screener PLUS, Sartorius, Gottingen, Germany).
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated at the indicated temperature and recovery was monitored by examination of GFP expression on a live-cell microscope (Incucyte SX5; Sartorius). The optimized protocol is presented in Supplementary Data 2.
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bioRxiv - Cell Biology 2023Quote: ... Cytotoxicity was quantified as reduction of GFP+ area over time via imagining in Incucyte S3 Live-Cell Analysis System (Sartorius).
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bioRxiv - Immunology 2024Quote: ... The collagen suspension was mixed carefully with the cells in a ratio of 4:1 and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... was monitored by imaging every 2 hours for 7 days at 10x magnification with the ‘phase’ channel using the Incucyte live-cell analysis system S3 (Sartorius). Automatic image segmentation and analysis of the phase contrast images was performed by the Incucyte base analysis software (version 2020B).
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bioRxiv - Physiology 2024Quote: ... Cultures were monitored for colony formation from 2 to 8 weeks after plating using Incucyte live cell imaging system (Sartorius). Wells with single colonies were harvested and cell cultures were expanded for making stocks and for DNA isolation ...
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bioRxiv - Synthetic Biology 2024Quote: ... The RFP+ count per well was quantified every two hours over a 40-hour span using IncuCyte S3 live-cell imaging (Sartorius). A375 cell growth was calculated as the number of RFP+ objects at a given time point ...
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bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
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bioRxiv - Immunology 2023Quote: ... measuring the total fluorophore area per well every 2h h using an Incucyte® S3 Live-Cell Analysis System (Sartorius). The data from each well were normalized to the first measurement (0h).
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation assays of the combination of GEM and clinical ATR inhibitors were performed using the Incucyte live-cell imaging platform (Sartorius). AsPC-1 cells were seeded at 5000 cells/well into 96-well Eppendorf flat bottom plates and allowed to attach overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5 x 103 cells/well were plated in FBS-free media in the upper chamber of a 96-well Incucyte ClearView cell migration plate (8 µm pore size; #4582 Sartorius). Cells were incubated at 37°C for 45 minutes to allow cells to settle on the membrane before adding RPMI-1640 media supplemented with 10% FBS to the bottom chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were taken every 15 min for 48 hrs on an Incucyte S3 Live-cell analysis system (Sartorius, 4X/0.2). The area covered by migrating cells was measured with the Incucyte analysis program (Sartorius).
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of confluency was assessed and automatically calculated based on the images acquired with IncuCyte® Zoom Live-Cell Analysis System (Sartorius) both at the day of compound addition (day 0 ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... 100µL of the combined LIVE/DEAD® assay reagent was then added to each well and incubate for 45 min before quantification of percentage of red and green cells using the IncuCyte® ZOOM (Sartorius) system and its proprietary ZOOM 2016A software.
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bioRxiv - Immunology 2021Quote: ... 7 L of Sf9 cell culture at a density of 2 × 106 cells/mL were infected with the baculovirus at a MOI of 3 using a Biostat B plus bioreactor (Sartorius, Germany). The following conditions were maintained during the culture period ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor cells transfected with plasmids containing eGFP were treated and co-cultured as described above in the IncuCyte® SC5 Live-Cell Analysis system (Sartorius). Plates were scanned with a 10x objective using phase contrast channel as well as the green fluorescent channel for 42 h every 6 h ...
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bioRxiv - Cell Biology 2020Quote: ... Live cell brightfield and fluorescent images for time-lapse experiments were acquired using the Incucyte® S3 Live Cell Analysis System (Essen Bioscience / Sartorius) running version 2019A (20191.1.6976.19779) ...
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bioRxiv - Neuroscience 2020Quote: ... Four fluorescent images per well with 300ms red channel acquisition time were taken every 15 minutes in total for three-hours using a 20x objective with the IncuCyte® S3 Live-Cell Analysis System (Sartorius). At the end of the assay ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
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bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
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bioRxiv - Microbiology 2022Quote: ... Fusion events were visualized for 24 h at 20 min intervals using the IncuCyte® S3 Live-Cell Analysis System (Sartorius). Phase contrast and GFP images (4 per well ...
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bioRxiv - Bioengineering 2023Quote: ... COS7 cells at a concentration of 60,000 cells/ml in Dulbecco’s Modified Eagle Medium (DMEM) with 1g/l D-glucose (Sartorius, 01-050-1A), supplemented with fetal bovine serum (Biological Industries ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants were harvested and clarified by centrifugation (3000xg for 20 minutes) or using Sartoclear Dynamics® Lab V (Sartorius). Supernatants were then filtered at 0.2 μm using Nalgene Rapid Flow sterile single using vacuum filter units (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... For the Incucyte assay, THP-1 or Jeko-1 cells were transduced with IncuCyte NucLight Red Lentivirus Reagent (EF-1 Alpha, Puro) (Sartorius, USA). All cell lines were repeatedly tested for the presence of mycoplasma contamination with a MycoReport Mycoplasma Detection Kit (Evrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 mL Tris buffer (50mM, pH 7.6) and afterwards lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Phase confluence cell images were obtained using a 10× objective lens every 6 hours for 168 hours and the average confluence of each well was calculated with the IncuCyte S3 Live Cell Analysis System (Sartorius, USA).
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bioRxiv - Cell Biology 2024Quote: ... One hundred microliters of the combined LIVE/DEAD® assay reagent was added to each well and incubated for 45 min before quantification of percentage of red and green cells using the IncuCyte® ZOOM (Sartorius) system and its proprietary ZOOM 2016A software.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Green fluorescence was immediately followed every 90 or 120 minutes with an Incucyte® S3 Live-Cell Analysis system (Sartorius, Germany). Images were analyzed with Incucyte software ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...