Labshake search
Citations for Sartorius :
201 - 250 of 321 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500, Sartorius AG, Göttingen, Germany), the samples were then brought to comparable protein concentrations and submitted to the Proteomics Facility of the University of Konstanz for protein mass fingerprinting ...
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bioRxiv - Immunology 2022Quote: ... The formulations were then diafiltrated against 100 x volume of Phosphate Buffered Saline (PBS) through a tangential-flow filtration (TFF) membrane with 10 kD molecular weight cut-offs (Sartorius Stedim Biotech), concentrated to a required concentration ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Biophysics 2022Quote: ... The peak fractions were collected and concentrated to <500 µL using a VivaSpin concentrator with 10 kDa cut-off (Sartorius Stedim Biotech). The concentrated protein was filtered through a 0.2 µm filter and injected on a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 30-KDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), flash-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... Thirty micrograms of protein resulting from each fraction was subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol 20 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5 mM tris(2-carboxyethylphosphine) ...
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bioRxiv - Microbiology 2021Quote: ... dispersed in deionized water with different pH were ultrasonically treated for 10 min at a frequency of 24 kHz (Sartorius labsonic M, Germany). The difference in the hydrodynamic diameter of the nanocomposites was investigated using a Zetasizer Nano-ZS-90 Dynamic Light Scattering (DLS ...
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bioRxiv - Immunology 2022Quote: ... or monitored in short time periods using a 10 x magnification in an Incucyte®S3 Live-Cell Analysis System (Sartorius, Göttingen, Germany). Image analysis was conducted with the machine learning software ilastik [80] using pixel and object classification to distinguish background from cell nuclei and to determine IRF3-GFP and H2B-mCherry colocalization ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteolytic digestion of sample extracts was carried out according to the FASP protocol33 using 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02) and loading 10 ug of protein resulting from each fraction ...
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bioRxiv - Biochemistry 2023Quote: Samples were subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol (Wisniewski et al. 2009) with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5 mM tris(2-carboxyethyl)phosphine) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... each female was weighed (MP8-1, Sartorius, Goettingen, Germany) and placed in a glass vial (7.5 mL ...
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bioRxiv - Neuroscience 2023Quote: ... annexin V green dye (Sartorius cat. #4642, 1:200) and a final concentration of 0.07uM TPPP PFFs were added to imaging media made with Fluorobrite DMEM (Fisher cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% penicillin streptomycin (Biological Industries – Sartorius 03-031-1B), 1x of ITS-X supplement (Thermo Fisher Scientific 51500-056) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% penicillin streptomycin (Biological Industries – Sartorius 03-031-1B), 1% Sodium Pyruvate (Biological Industries – Sartorius 03-042-1B) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% Sodium Pyruvate (Biological Industries – Sartorius 03-042-1B), 1% non-essential amino acids (Biological Industries – Sartorius 01-340-1B ...
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bioRxiv - Bioengineering 2023Quote: ... A 1:500 dilution of Annexin V-Orange (Sartorius) was added to the cells to monitor MHV induced cell lysis and cell death ...
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bioRxiv - Biochemistry 2024Quote: ... 1% l-glutamine (Biological Industries – Sartorius, 03-020-1B), and 1% Penicillin Streptomycin Solution (Biological Industries – Sartorius ...
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bioRxiv - Neuroscience 2024Quote: ... then labeled with the pHrodo Red Cell Labeling Kit for Incucyte (4649; Sartorius, Bohemia, NY) with a final concentration of 125 ng/mL dye following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines tested negative for mycoplasma contamination using the Microsart AMP Mycoplasma Kit (Sartorius).
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bioRxiv - Biophysics 2020Quote: ... The flow-through of the column was concentrated (Vivaspin®, mwco 10 or 30 kDa for single SBD or tandem mutants, Sartorius; ~ 5 mg/ml), dialyzed in buffer A supplemented with 50 % glycerol ...
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bioRxiv - Molecular Biology 2022Quote: ... were mixed together as well as eluates from samples (Pho92-HTP x 2 reps + Pho92-HTP ime4Δ/Δ x 2 reps) before concentration with Vivacon® 500 filtration cartridges (10 kDa MWCO; Sartorius, Cat# VN01H02) to a final volume of 120 μL ...
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bioRxiv - Bioengineering 2023Quote: ... Batch reactors were incubated for 10 days at a mesophilic temperature of 35°C in a Certomat® BS1 incubator (Sartorius Stedim Biotech, Germany) under continuous agitation (145 rpm) ...
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bioRxiv - Physiology 2024Quote: ... Detection was done with the EZ-ECL Chemiluminescence Detection kit for HRP (20-500-120, Sartorius).
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bioRxiv - Immunology 2023Quote: ... Plates were imaged every 1 hr using Incucyte S3 (Sartorius) for 12 hr recording 3 fields/well ...
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Biochemistry 2022Quote: ... POLD1 was concentrated to 1 mL using Vivaspin centrifugal concentrator (Sartorius) and loaded onto a HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% non-essential amino acids (Biological Industries – Sartorius 01-340-1B) and 0.1 mM β-mercaptoethanol (Thermo 31350010).
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bioRxiv - Biochemistry 2024Quote: ... and 1% Penicillin Streptomycin Solution (Biological Industries – Sartorius, 03-031-1B) under standard conditions (37 °C and 5% CO2 in a humidified incubator) ...
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bioRxiv - Cancer Biology 2022Quote: ... using and the pHrodo-stained B cells (IncuCyte® pHrodo® Red Cell Labelling Kit for Phagocytosis, Sartorius). Following phagocytosis assay ...
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bioRxiv - Biochemistry 2023Quote: ... At each of the specified time points cell viability was measured using XTT-based Cell Proliferation Kit (Sartorius), according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... ImageLock plates (96-well; #4379) as well corresponding Woundmaker Incucyte® Cell Migration Kit (#4493) were from Sartorius. All other consumables were from Sigma-Aldrich except where noted.
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bioRxiv - Bioengineering 2021Quote: ... After concentration by centrifugation on Vivaspin (10000 g mol-1 MWCO; Sartorius), we further purified to remove endotoxins with Pierce high capacity endotoxin removal spin columns (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... A 1:2,000 dilution of Incucyte® NucLight Red dye (Sartorius 4717) was added to stain nuclei and images were obtained every 15 min ...
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bioRxiv - Neuroscience 2019Quote: ... Total supernatants were treated with benzonase nuclease (12.5 U/ml) and subjected to two rounds of column purification using the Vivapure AdenoPACK 100RT kit (Sartorius). The final viral stocks were stored at −80°C in storage solution (10 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... as well as 1:500 of Nuclight Rapid Red Dye (Sartorius, cat #4717) were added to each well ...
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bioRxiv - Plant Biology 2021Quote: ... by weighing the pots with a precision of +/- 1 g (Sartorius, Aubagne, France), until the water content in the pots reached 15% field capacity (corresponding to a weight loss of 144 g) ...
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bioRxiv - Bioengineering 2022Quote: ... with a length of 1 cm were weighted using a microbalance (Sartorius, Germany). Then ...
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bioRxiv - Cell Biology 2022Quote: ... concentrated in 1 mL in a Vivaspin Turbo 4 (30’000 MWCO, Sartorius VS04T22) and further purified on a Superdex 75 10/300 gel filtration column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... concentrated to 1-5 mg/ml by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at -80°C.
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) and concentrated (∼100 µM) using centrifugal filters (Vivaspin 500, Sartorius). In the apo condition ...
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bioRxiv - Immunology 2023Quote: ... resuspended in phenol red-free RPMI containing Cytotox Green (1/4000 diluted, Sartorius). Tumor cells were washed twice with phenol red-free RPMI ...
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bioRxiv - Cancer Biology 2023Quote: The cell viability percentage for each concentration was assessed with an XTT-based cell proliferation kit (Sartorius, Beit Haemek, Israel), then entered into SynergyFinder web application (v 2.0) ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM EDTA) for 1.5 min at 2000 rpm in the Mikro-Dismembrator (Sartorius). Resulting crude extracts were subjected to sonication in 30 ml buffer and afterwards centrifuged twice at 13.000 rpm and 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... to a minimum response value of 1 nm on the Octet Red96 System (Sartorius). A baseline response was established in assay buffer prior to each association ...
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bioRxiv - Neuroscience 2022Quote: ... to a minimum response value of 1 nm on the Octet Red96 System (Sartorius). For measurements of amyloid beta ...
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bioRxiv - Neuroscience 2022Quote: ... to a minimum response value of 1 nm on the Octet Red96 System (Sartorius). Purified mTfR or VHH clones were diluted into assay buffer at the specified concentrations ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a slow filter paper (#293; 1–2 µm Sartorius, Thermo Fisher Scientific), and then used to determine electrical conductivity (EC) ...
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bioRxiv - Molecular Biology 2024Quote: ... and ZIP cells (1:1000 dilution, #4642 and #4641, Sartorius, Ann Arbor, MI - USA) immediately before time 0 of IncuCyte scan ...