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Citations for Sartorius :
201 - 250 of 275 citations for Adenovirus Type 5 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The fractions containing MTG variants were concentrated to a volume of approximately 5 mL using ultrafiltration membrane of 10 kDa MWCO (Vivaspin Turbo 15, Sartorius). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Immunology 2020Quote: ... The EV pellet was resuspended in 2 ml PBS and stored at −80 prior to further concentration using a 5 kDa MW cut-off vivaspin (Sartorius). The protein content of EVs was quantified using Qubit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Biotinylated-NIH-CoVnb-112 was diluted into assay buffer at 5 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated NPNA peptides were diluted to 5 μg/mL in kinetics buffer (KB) and immobilized onto streptavidin BLI biosensors (Sartorius); His-tagged rsCSP was diluted to ~1 μg/mL in KB and loaded onto Ni-NTA biosensors ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 104 cells were seeded in each well of an Incucyte® ImageLock 96-well plate (Sartorius, Catalog #: 4379) in 100 μL/well of either complete RPMI or DMEM ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing biotinylated primer bound to monovalent streptavidin were subsequently annealed to a 5’ biotinylated DNA template before binding to streptavidin biosensors (Sartorius). 200 nM of DNA substrates were added in the loading step ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% nonfat dry milk) to 100 nM and immobilized for 60 s to anti-human IgG Fc capture biosensors (Sartorius). Sensors were transferred to assay buffer for 30 s to set the baseline ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing FKBP12 F36V were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of FKBP12 F36V was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Immunology 2023Quote: ... Mouse antibodies (7.5 μg/mL anti-OVA and 5 μg/mL anti-RBD) were captured on pre-conditioned anti-mouse IgG Fc-capture biosensors (AMC, Sartorius) for 600 s ...
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bioRxiv - Immunology 2023Quote: ... The cocultures were incubated for 48 hours in a humidified incubator (37 °C, 5% CO2) equipped with an Incucyte (Sartorius) for continuous monitoring of cell proliferation and target cell killing ...
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3–5 x103 cells were seeded in 96-well plates and viable cell numbers were determined using the Incucyte (Sartorius) Adherent-Cell-by-Cell module ...
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bioRxiv - Developmental Biology 2024Quote: ... for a total of 15-20 minutes with trituration every 5 minutes using 200 μl low bind tips (Sartorius, 790101F). Papain was inactivated using an Ovomucoid inhibitor (Worthington Papain Dissociation Kit ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Immunology 2022Quote: Binding kinetics of WS6 IgG and related S2-stem helix antibodies to diverse CoV 2P-stabilized spike proteins (S2Ps) were measured by biolayer interferometry using an Octet HTX instrument (Sartorius). All assays were performed in tilted 384-well plates (Geiger Bio-one ...
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bioRxiv - Biochemistry 2020Quote: ... glycerol was added and the protein concentrated to 20 – 30 mg/mL in a 10 kDa MWCO spin concentrator (Vivaspin, Sartorius).
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bioRxiv - Microbiology 2020Quote: ... The protein was loaded on a HiLoad 16/600 Superdex 200 pg column pre-equilibrated with storage buffer and the fractions containing pure Rel protein were collected and concentrated in a Vivaspin turbo 15 (Sartorius). The purity of protein preparations and the absence of RNA were assessed by SDS-PAGE and on a 1% agarose gel (in 1 × TAE buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...
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bioRxiv - Immunology 2021Quote: ... Purified S proteins were exchanged to storage buffer and then filtered through 0.22 um filter (Sartorius Stedim Biotech, Göttingen, Germany). The concentration of purified protein was determined by ELISA ...
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Immunology 2021Quote: ... S2K146 and respective UCA Ab were prepared at 3 μg/ml and immobilized on pre-hydrated protein-A biosensors (Sartorius) for 75 sec ...
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bioRxiv - Immunology 2022Quote: KD measurements of the Pertuzumab and Trastuzumab IgMs to their Her2 antigen (Cat: 10004-HCCH, SinoBiological) were performed by first immobilizing them on Protein L (Cat: 18-5085, Sartorius) and CaptureSelect™ Biotin Anti-IgM Conjugate (Cat ...
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bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Plant Biology 2021Quote: ... the pH of the hydroponic culture media of the 5 treatments was measured using a pH meter (PB-10, Sartorius, Germany).
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bioRxiv - Plant Biology 2020Quote: ... Material for analyses was obtained from seeds pre-cooled for 15 min in 5-mL Teflon capsules (Sartorius GmbH, Göttingen, Germany), containing a 10 mm-diameter agate bead ...
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bioRxiv - Biochemistry 2022Quote: ... A template with a biotin-TEG at the 5’-end was annealed to the primers (Table 2) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). Primers were added at two-fold molar excess over the template ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Microbiology 2021Quote: ... using a centrifugation tube with an insert containing a molecular weight cut-off filter (5 kDa, Sartorius Stedim Biotech, Goettingen, Germany). Each HMW fraction was purified by flushing with 20 ml of water ...
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bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Immunology 2023Quote: ... the mNeonGreen signals from infected MRC-5 cells were monitored for 1 week using the Incucyte™ Live-Cell analysis system (Sartorius). Additionally ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
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bioRxiv - Microbiology 2019Quote: ... Each mixture containing 100μg proteins was loaded onto a 10 kDa cut-off spin column (Vivacon 500, Sartorius AG, Goettingen, Germany). The lysate was adjusted to 8M urea by centrifuging to remove SDS and low-molecular-weight material ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG) and passed through a spin-out column of Bio-Gel P-6 equilibrated with the protein storage buffer (0.1 M Hepes-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Physiology 2022Quote: ... 5 μL) was controlled by weighing with precision to 0.01 mg using a Sartorius CP 225 D balance (Sartorius AG, Goettingen, Germany). The solutions were frozen and thawed according to the following protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated at 37°C with 5% CO2 during 24h in the IncuCyte S3 live-cell imaging and analysis system (Essen BioScience, Sartorius, Göttingen, Germany). Images were acquired using a 10X objective and 440-480 nm Excitation / 504-544 nm Emission filters ...