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Citations for Sartorius :
201 - 250 of 273 citations for 6 AMINO 2 5 DINITROPYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Flow-through and wash fractions were pooled and concentrated using Vivaspin 15R concentrators (2 kDa MWCO HY, Sartorius).
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bioRxiv - Molecular Biology 2021Quote: ... diluted to a final Env trimer concentration of 2 mg/mL and filtered using 0.45 + 0.2 µm Sartobran 300 filter capsules (Sartorius). As final steps ...
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bioRxiv - Immunology 2020Quote: ... GFP fluorescent intensity was detected every 2 hours using the IncuCyte S3 Live-Cell analysis system (Sartorius, Germany). Total integrated GFP intensity per well was used as a quantitative measure of viable target cells ...
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bioRxiv - Biochemistry 2020Quote: ... Obtained fractions were analyzed by SDS PAGE and fractions containing proKLK14 were concentrated with Vivaspin® 2 (Sartorius) and further purified on Superdex s75 pg (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing FimH-FimC were dialyzed against buffer A and were concentrated using Vivaspin 2 (Sartorius, Göttingen, Germany). For cryo-EM ...
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bioRxiv - Cancer Biology 2023Quote: ... Parental and MCT1-KO MIA PaCa-2 cells were transduced with Lentiviral NucLight Red and Green vectors (Sartorius), respectively ...
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bioRxiv - Immunology 2024Quote: The binding kinetics of ZBP1-Zα1Zα2 with SARS-CoV-2 RNAs were evaluated using the Octet RED96 instrument (Sartorius). First ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Plant Biology 2021Quote: ... the pH of the hydroponic culture media of the 5 treatments was measured using a pH meter (PB-10, Sartorius, Germany).
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bioRxiv - Plant Biology 2020Quote: ... Material for analyses was obtained from seeds pre-cooled for 15 min in 5-mL Teflon capsules (Sartorius GmbH, Göttingen, Germany), containing a 10 mm-diameter agate bead ...
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bioRxiv - Microbiology 2021Quote: ... using a centrifugation tube with an insert containing a molecular weight cut-off filter (5 kDa, Sartorius Stedim Biotech, Goettingen, Germany). Each HMW fraction was purified by flushing with 20 ml of water ...
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bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Immunology 2023Quote: ... the mNeonGreen signals from infected MRC-5 cells were monitored for 1 week using the Incucyte™ Live-Cell analysis system (Sartorius). Additionally ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
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bioRxiv - Bioengineering 2020Quote: Batch experiments were performed using a 2 L bioreactor equipped with a Sartorius BIOSTAT B® fermentation controller (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Molecular Biology 2021Quote: ... Triton X-100 and incubation at RT for 60-120 mins and solutions sterile filtered using 0.45 µm Sartopore 2 filters (Sartorius). Triton X-100 inactivated solutions were batch-wise loaded onto a MAb PGT145 immunoaffinity column for capture of ConM and ConS Env trimers ...
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bioRxiv - Biophysics 2021Quote: ... Following sonication, DNA impurities were digested (DNase: Fisher Optizyme, 2 U/ml) and the supernatant was filtered (0.45 μm, Sartorius) and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: Protein-containing fractions were combined and concentrated to 0.5 ml using a 50-kDa molecular weight cutoff (MWCO) Vivaspin 2 concentrator (Sartorius), and desalted by buffer exchange on a PD MiniTrap G-25 column (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... samples were further concentrated to ~1-2 mg/mL with a 100 kDa MWCO Vivaspin 500 centrifugal concentrator (Sartorius) at 12000 ×g ...
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bioRxiv - Systems Biology 2023Quote: ... The wells were imaged every 2 hours for 120 hours and cell confluence was measured with Incucyte ZOOM (Sartorius).
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bioRxiv - Bioengineering 2022Quote: Fed-batch bioreactor experiments were performed in 2-L bench top glass fermenters (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated EVs were subsequently concentrated to a volume of 100 ul using a 2 ml 100,000 kDa Vivaspin column (Sartorius).
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bioRxiv - Physiology 2022Quote: ... 5 μL) was controlled by weighing with precision to 0.01 mg using a Sartorius CP 225 D balance (Sartorius AG, Goettingen, Germany). The solutions were frozen and thawed according to the following protocol ...
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bioRxiv - Immunology 2019Quote: ... Worm culture supernatants collected over 1 week were further concentrated using centrifugal protein concentrators with a 5 kDa MWCO (Vivaspin, Sartorius, Göttingen, Germany) to obtain the final ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated at 37°C with 5% CO2 during 24h in the IncuCyte S3 live-cell imaging and analysis system (Essen BioScience, Sartorius, Göttingen, Germany). Images were acquired using a 10X objective and 440-480 nm Excitation / 504-544 nm Emission filters ...
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bioRxiv - Immunology 2022Quote: ... The BM cells were centrifuged for 5 minutes at 400 x g and then resuspended in Dulbecco’s Modified Eagle Medium (DMEM) (Sartorius, 01-052-1A) supplemented with 10% (v/v ...
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bioRxiv - Genetics 2023Quote: ... The eluate was washed 3 times with PBS containing 5% Sorbitol and concentrated with Vivaspin 20 100K concentrator (Sartorius Stedim, Bohemia, NY). Vector titer was determined by qPCR with primers and labeled probe targeting the ITR sequence (Aurnhammer et al. ...
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bioRxiv - Biochemistry 2023Quote: The perfusion cell culture experiments were conducted in a 5 L glass bioreactor using a Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 2 L ...
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bioRxiv - Molecular Biology 2023Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 30-KDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), flash-frozen in liquid nitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... The plate was centrifuged for 5 minutes at 100g and then placed in the IncuCyte S3 Live-Cell Analysis System (Sartorius, G_ttingen, Germany) and stored at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell monolayer was scraped and migration was monitored via time-lapse image acquisition every 2 hours via the IncuCyte ZOOM imaging platform (Sartorius). Migration rates were calculated as relative wound density over time for wildtype and mutant cell lines ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... Fragmented gDNA was obtained by beat-beating the intact gDNA for 12 minutes using 2 mm diameter glass beads in a Mikro-Dismembrator U (Sartorius) at 2000 rpm ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Images and confluence data were acquired every 2 h for ~200 h using the IncuCyte Live Cell Analysis Imaging System (Sartorius). The Area Under Curve (AUC ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Cell Biology 2023Quote: Isolated ECs were seeded onto 96 well-plates pre-coated with fibronectin in EGM-2 and the proliferation assay was performed for 60 hours in the Incucyte proliferation assay according to (21) (Sartorius). The analysis of the cell proliferation was performed by the Incucyte Basic Analyzer software.
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bioRxiv - Bioengineering 2022Quote: ... Four images were recorded per well every 2 h and analyzed using IncuCyte cell by cell analysis software module (Sartorius). The killing potency of the CAR-T cells was assessed by comparing the percentage of Annexin V positive target cells over time relative to the total number of target cells.
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bioRxiv - Bioengineering 2023Quote: ... This experiment was carried out in a commercial stirred tank bioreactor (STBR) of 2 l total volume (Sartorius AG, Germany) and a working volume of 1 l was used ...
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bioRxiv - Molecular Biology 2023Quote: ... The monodisperse peak of the complex was collected and concentrated with a 50 kDa cut-off concentrator (Vivaspin 2, Sartorius) and subsequently used for crystallization in LCP.
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Cell Biology 2023Quote: ... The remaining vesicle pellets were resuspended in 2 mL sterile 1× PBS and sterile filtered with 0.20 µm syringe filters (Carl Roth, Sartorius VS0271).
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bioRxiv - Synthetic Biology 2023Quote: ... Four images per well were acquired at 10x magnification in the green channel (200 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hrs using an Incucyte (Sartorius). A cytotoxicity index was calculated by dividing total green fluorescence intensity per mm2 at every time point by the intensity per mm2 at the first time point ...
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bioRxiv - Synthetic Biology 2023Quote: ... One image per well was acquired at 4x magnification in the green channel (100 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hr using an Incucyte (Sartorius).
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bioRxiv - Physiology 2024Quote: ... Cultures were monitored for colony formation from 2 to 8 weeks after plating using Incucyte live cell imaging system (Sartorius). Wells with single colonies were harvested and cell cultures were expanded for making stocks and for DNA isolation ...
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bioRxiv - Biochemistry 2020Quote: ... and the filtrate concentrated using a ultrafiltration apparatus with a 5 kDa molecular mass cut-off (Vivaflow® 200 cassette, Sartorius Stedim Biotech). The concentrate was separated by reversed phase (RP-HPLC ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples weighing 5-15mg were dispensed in standard aluminum pans (DSC consumables incorporated, Minnesota, USA) using a microbalance (Sartorius 3.6P microbalance, Göttingen, Germany) and sealed using standard aluminum lids ...
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bioRxiv - Genomics 2024Quote: The human T lymphoblast Sup T1 cell line was cultured at 37°C under a 95% air and 5% CO2 atmosphere in Roswell Park Memorial Institute medium (RPMI 1640 medium; Sartorius #01-100-1A) supplemented with 10% fetal bovine serum (FBS ...