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Citations for Sartorius :
151 - 200 of 321 citations for Acetaldehyde 3a 4 5 6 7 7a hexahydro 4 7 methano 1H inden 5 yl oxy since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... the DAN-G single knockout cells was co-cultured with TCR-T cells at a 4:1 T cell:tumor cell ratio and imaged every 4 hours at 10X magnification using an Incucyte S3 machine (Sartorius).
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bioRxiv - Biophysics 2024Quote: ... 4 μM of 6xHis-tagged ASF1A1-172 was immobilized on nickel-charged tris-nitriloacetic acid (Tris-NTA) biosensor (Sartorius) for the 90 seconds ...
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bioRxiv - Microbiology 2021Quote: ... Samples were held separately in 5 L of sterile marine water (filtered through the 0.22 μm Sartorius filter) for 2 h at 5°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted by centrifugation (16,000g, 5 min) and the supernatant passed through a 0.22 μm nitrocellulose filter (Sartorius). Proteins were precipitated with 10% trichloroacetic acid (TCA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were placed in the Incucyte SX5 and maintained at 37°C and 5% CO2 (Sartorius, Göttingen, Germany) for detection and automated analysis of cell growth/proliferation ...
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bioRxiv - Microbiology 2024Quote: ... samples were clarified using a syringe with a filter cascade (5 µm, 1.2 µm, 0.8 µm, 0.45 µM, Sartorius AG). The cell pellets were not further analysed ...
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bioRxiv - Bioengineering 2024Quote: ... the shake flask culture was centrifuged for 5 min at 110 xg (Model 6K15, Sartorius AG, Göttingen, Germany). The supernatant was discarded and the cell pellet was resuspended in an appropriate volume of medium to generate a stock cell solution with an estimated concentration of 6.6 × 106 cells mL−1 for A and of 2.2 × 107 cells mL−1 for B ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Filtered HES was concentrated using a VivaSpin 20 centrifugal concentrator with a 5 kDa molecular weight cutoff (Sartorius). EVs were purified from concentrated HES by ultracentrifugation at 100,000 x g for 90 mins at 4 °C in polyallomer tubes (Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... The flow-through fraction was collected and concentrated to 5 mL with centrifugal filter devices (Vivaspin 20, Sartorius). A second SEC was performed on an Äkta prime plus FPLC (Cytiva) ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent and phase contrast images are collected at intervals of 4 hours or shorter throughout the study in the Incucyte S2 Live Cell Analysis System (Essen/Sartorius) with temperature and environmental control ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were spun at 15,000g for 15 min at 4°C and supernatants were then passed through a regenerated cellulose filter (4mm Ø, Sartorius). Samples were then dried under N2 flow at 40°C and resuspended in 125µl of ice-cold methanol/water 70:30 for subsequent analyses.
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to hIP3R-3 were combined and concentrated to 4 mg/mL using a 100 kDa centrifugal filter (Sartorius). The concentrated sample was then centrifuged at 70,000 rpm using a S100AT rotor (Thermofisher) ...
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bioRxiv - Biophysics 2020Quote: ... MgCl2 and EGTA were removed by 4 times buffer exchange using a Vivaspin 500.5 molecular weight cut-off (Sartorius, Göttingen). Samples were loaded onto a SDS-PAGE gel to confirm phosphorylation.
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bioRxiv - Neuroscience 2020Quote: ... cells and media were collected and this mixture was centrifuged at 3700xg at 4°C to remove debris and then concentrated by ultrafiltration using Vivaflow 200 (Sartorius). The purified AAV particles were collected after running the viral concentrate through an iodixanol column (Opti-Prep density gradient medium ...
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bioRxiv - Immunology 2021Quote: ... The purified Spike and RBD recombinant proteins were then buffer exchanged into sterile PBS by centrifuging at 4000xg for 30 minutes at 4 °C in a 10,000 MWCO Vivaspin centrifugal concentrator (Sartorius, Germany) and stored at –80 °C ...
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bioRxiv - Cell Biology 2023Quote: MuSCs were freshly isolated and seeded on 0.2% gelatin-coated 96 well plates at a density of 2,000 cells per well in growing conditions and incubated for 4 days in an IncuCyte SX5 Live-Cell Analysis System (Sartorius) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... Protein was eluted using similar buffer with 300 mM imidazole and concentrated by centrifugation with 4 kDa (AlgF) or 30 kDa (AlgJ and AlgX) cutoff Vivaspin Turbo centrifugal concentrators (Sartorius). His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... The collagen suspension was mixed carefully with the cells in a ratio of 4:1 and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the samples were moved back and forth every 4 h for a period of 8 h between the blue light source and the IncuCyte reader (Sartorius) to allow the acquisition of the phase and the red channels ...
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bioRxiv - Microbiology 2024Quote: ... and concentrated by centrifugation at 1500 x g at 4 °C using ultrafiltration devices with a filter cut-off at 100 kDa (Centrisart, Sartorius) [37] ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were further purified by size exclusion chromatography using Superdex-75 or −200 (Cytiva) at 4°C and concentrated by Vivaspin (Sartorius) concentrators at 4°C (Kar9-Bim1 ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Microbiology 2022Quote: ... The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm, Sartorius Stedim Biotech GmbH). The biomass was washed on the filter with 10 mL of 60% methanol and stored at -70 °C until metabolite extraction.
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Cancer Biology 2022Quote: ... Medium containing DMSO or increasing concentration of test compound was added to each well and spheroid growth was monitored for 4 days post-treatment using the IncuCyte® Spheroid Analysis system (Sartorius). Images from 4 days post-treatment and day 0 were analyzed using the IncuCyte® Spheroid Analysis software module ...
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bioRxiv - Bioengineering 2024Quote: ... Cell debris was removed via centrifugation (5000g, 30 min, 4°C) and filtration of the supernatant via Filtropur 0.2 µm filters (Sartorius, Göttingen, Germany). The soluble protein fraction was then loaded on a HisTrap HP column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... and BA.2+R493Q RBDs were diluted to 5 μg/mL and immobilized onto pre-hydrated streptavidin biosensors (Sartorius) to a level of 1 nm total shift ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the protein-RNA complex were concentrated to 400-700 uM with Vivaspin 5-mL centrifugal devices with 10,000 MWCO (Sartorius). Before the measurements a 10% molar excess of RNA was added to saturate the protein as well as 10% v/v D2O ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 by repeated ultracentrifugation using a Vivaspin 500 filter device with a cutoff of 30 kDa (Sartorius) at 4 °C and afterwards concentrated ...
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bioRxiv - Biophysics 2023Quote: ... using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius), with assays designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Cell Biology 2024Quote: ... pollen contained in 5 ml suspension was vacuum-filtrated onto a round polyamide membrane filter (diameter 5 cm, pore size 0.45 µm; Sartorius) and transferred onto 3.5 ml solid (0.25 % phytagel [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was incubated at 37°C and 5% CO2 and imaged using an Incucyte Live-Cell imager (Sartorius). The red channel used an acquisition time of 400 milliseconds for all replicates and samples ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was sterile-filtered using 0.2µm pore size syringe filters with low protein binding (Sartorius, ref.:16534-5). The obtained FW was stored at -80°C until further analysis.
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Microbiology 2024Quote: ... VHH-His (or sACE2-His) (5 µg/ml) diluted into Sartorius’s PBS working buffer were immobilized onto Ni-NTA biosensors (Sartorius), which were pre-equilibrated in the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius), with assays designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Cancer Biology 2024Quote: ... and ABTR#2 cells were infected at an MOI of 3-5 with the IncuCyte Nuclight Red (#4476 Sartorius) or IncuCyte Cell Cycle Green/Red Lentivirus Reagent (#4779 ...
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bioRxiv - Synthetic Biology 2024Quote: ... strains were cultivated by fed-batch fermentation in a 5 L bioreactor (BIOSTAT® B-DCU, Sartorius, Goettingen, Germany). All fermentations were carried out in duplicate ...
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bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
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bioRxiv - Microbiology 2020Quote: ... liquid phage suspension was centrifuged at 4000 g for 10 min at 4°C and the supernatant was subsequently filter sterilised using a 0.45 μm pore sized membrane filter (Sartorius Minisart®, 16555). The filtrate phage lysate was stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: The purified proteins were concentrated and exchanged to 10 mM sodium citrate buffer (pH 5.0) using Vivaspin® Turbo 4 ultrafiltration units with a 5000 MWCO PES membrane (Sartorius; Cat.# VS04T11). The purity and concentration of each PcaLOOL was assessed by SDS-PAGE and the Pierce™ BCA Protein Assay Kit ...
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bioRxiv - Cell Biology 2024Quote: ... Plates were imaged within a 4-hour interval in a multi-spheroid mode using an Incucyte S3 high-content screening system (Sartorius, Bohemia, NY). Average cyst areas (μm2 ...
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bioRxiv - Neuroscience 2024Quote: ... 3-4 organoids from each hPSCs line were transferred to a 60 mm culture dish containing 4 mL of pre-warmed Earle’s Balanced Salt Solution (EBSS) (Sartorius; 02-010-1A). The organoids were manually dissected into small pieces and transferred into a new 60 mm dish containing 2.5 mL of Enzymatic dissociation solution [composed of 27.3 U/mL papain solution (Worthington ...
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bioRxiv - Immunology 2024Quote: ... Assay cells were fixed with 4% paraformaldehyde (PFA) before microsphere uptake readouts (“phagoscore”) were quantified using the iQue Screener Plus (Sartorius, Göttingen, Germany).
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bioRxiv - Cell Biology 2021Quote: ... Brightfield images were taken every 2 hours for 5 days and the percentage confluency calculated using an Incucyte Live-Cell analysis system (Sartorius).
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bioRxiv - Biophysics 2022Quote: BLI analyses were performed at 25°C at an acquisition rate of 5 Hz averaged by 20 using an Octet© BLItz System (FortéBio/Sartorius) with assays were designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Biochemistry 2020Quote: ... 5% ethylene glycol), followed by buffer exchange to (30 mM Tris/HCl, pH 8.0) with Vivaspin centrifugal concentrator (Sartorius, Germany). The final phosphoprotein concentrations were adjusted to 1.5 mg/ml (80 μM) ...
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bioRxiv - Biochemistry 2021Quote: The dialysed proteins were concentrated to a <5 mL volume using a 30 kDa MWCO Vivaspin 20 centrifugal concentrator (Sartorius) at 5000 g ...
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bioRxiv - Cell Biology 2022Quote: ... individual frames of cells growing under normal culture conditions were acquired every 5 minutes for 24 hours using an IncuCyte ZOOM live-cell analysis system (Sartorius) equipped with a 20 x objective.
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bioRxiv - Bioengineering 2020Quote: ... The fractions containing MTG variants were concentrated to a volume of approximately 5 mL using ultrafiltration membrane of 10 kDa MWCO (Vivaspin Turbo 15, Sartorius). Subsequently ...