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Citations for Sartorius :
151 - 200 of 503 citations for 6 METHYL 4 4 NITRO PHENYL 2 OXO 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The samples were first concentrated from 2 L down to 40 mL using the vivaflow 200 (Sartorius). The samples were then concentrated from 40 mL to ∼2 mL using vivaspin 20 ultrafiltration units (Sartorius) ...
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bioRxiv - Cell Biology 2022Quote: ... the Spectrum polyP was desalted using a Vivacon 500 2 kDa cutoff spin filter (Sartorius, Bohemia, NY) at 7500 x g for 60 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The medium was filter sterilized using a 0.2 μm Sartopore 2 filter unit (Sartorius Stedim, Goettingen, Germany).
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bioRxiv - Immunology 2022Quote: ... followed by the removal of 2-MEA by buffer-exchanging to PBS using 100kDa Vivaspin6 columns (Sartorius) and storing overnight at 4 °C to allow for reoxidation of the disulfide bonds.
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bioRxiv - Synthetic Biology 2023Quote: ... The cell culture was inoculated at OD600 0.3 in the 2 L bioreactor (Biostat B, Sartorius, Germany) including the 1 L modified M9 minimal medium ...
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bioRxiv - Genetics 2023Quote: ... The eluate was washed 3 times with PBS containing 5% Sorbitol and concentrated with Vivaspin 20 100K concentrator (Sartorius Stedim, Bohemia, NY). Vector titer was determined by qPCR with primers and labeled probe targeting the ITR sequence (Aurnhammer et al. ...
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bioRxiv - Microbiology 2021Quote: ... Flow-through and wash fractions were pooled and concentrated using Vivaspin 15R concentrators (2 kDa MWCO HY, Sartorius).
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bioRxiv - Molecular Biology 2021Quote: ... diluted to a final Env trimer concentration of 2 mg/mL and filtered using 0.45 + 0.2 µm Sartobran 300 filter capsules (Sartorius). As final steps ...
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bioRxiv - Immunology 2020Quote: ... GFP fluorescent intensity was detected every 2 hours using the IncuCyte S3 Live-Cell analysis system (Sartorius, Germany). Total integrated GFP intensity per well was used as a quantitative measure of viable target cells ...
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bioRxiv - Biochemistry 2020Quote: ... Obtained fractions were analyzed by SDS PAGE and fractions containing proKLK14 were concentrated with Vivaspin® 2 (Sartorius) and further purified on Superdex s75 pg (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Parental and MCT1-KO MIA PaCa-2 cells were transduced with Lentiviral NucLight Red and Green vectors (Sartorius), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing FimH-FimC were dialyzed against buffer A and were concentrated using Vivaspin 2 (Sartorius, Göttingen, Germany). For cryo-EM ...
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bioRxiv - Immunology 2024Quote: The binding kinetics of ZBP1-Zα1Zα2 with SARS-CoV-2 RNAs were evaluated using the Octet RED96 instrument (Sartorius). First ...
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bioRxiv - Immunology 2020Quote: ... concentrated to 1-5 mg/ml by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at -80°C.
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bioRxiv - Bioengineering 2020Quote: Batch experiments were performed using a 2 L bioreactor equipped with a Sartorius BIOSTAT B® fermentation controller (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Molecular Biology 2021Quote: ... Triton X-100 and incubation at RT for 60-120 mins and solutions sterile filtered using 0.45 µm Sartopore 2 filters (Sartorius). Triton X-100 inactivated solutions were batch-wise loaded onto a MAb PGT145 immunoaffinity column for capture of ConM and ConS Env trimers ...
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bioRxiv - Biophysics 2021Quote: ... Following sonication, DNA impurities were digested (DNase: Fisher Optizyme, 2 U/ml) and the supernatant was filtered (0.45 μm, Sartorius) and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: Protein-containing fractions were combined and concentrated to 0.5 ml using a 50-kDa molecular weight cutoff (MWCO) Vivaspin 2 concentrator (Sartorius), and desalted by buffer exchange on a PD MiniTrap G-25 column (Cytiva ...
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bioRxiv - Systems Biology 2023Quote: ... The wells were imaged every 2 hours for 120 hours and cell confluence was measured with Incucyte ZOOM (Sartorius).
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bioRxiv - Bioengineering 2022Quote: Fed-batch bioreactor experiments were performed in 2-L bench top glass fermenters (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated EVs were subsequently concentrated to a volume of 100 ul using a 2 ml 100,000 kDa Vivaspin column (Sartorius).
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bioRxiv - Microbiology 2023Quote: ... After concentration (Vivaspin 6 Concentrator, Sartorius Stedim Biotech) and desalting (PD 10 Desalting Columns ...
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell monolayer was scraped and migration was monitored via time-lapse image acquisition every 2 hours via the IncuCyte ZOOM imaging platform (Sartorius). Migration rates were calculated as relative wound density over time for wildtype and mutant cell lines ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... Fragmented gDNA was obtained by beat-beating the intact gDNA for 12 minutes using 2 mm diameter glass beads in a Mikro-Dismembrator U (Sartorius) at 2000 rpm ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Images and confluence data were acquired every 2 h for ~200 h using the IncuCyte Live Cell Analysis Imaging System (Sartorius). The Area Under Curve (AUC ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Cell Biology 2023Quote: Isolated ECs were seeded onto 96 well-plates pre-coated with fibronectin in EGM-2 and the proliferation assay was performed for 60 hours in the Incucyte proliferation assay according to (21) (Sartorius). The analysis of the cell proliferation was performed by the Incucyte Basic Analyzer software.
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bioRxiv - Bioengineering 2022Quote: ... Four images were recorded per well every 2 h and analyzed using IncuCyte cell by cell analysis software module (Sartorius). The killing potency of the CAR-T cells was assessed by comparing the percentage of Annexin V positive target cells over time relative to the total number of target cells.
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bioRxiv - Bioengineering 2023Quote: ... This experiment was carried out in a commercial stirred tank bioreactor (STBR) of 2 l total volume (Sartorius AG, Germany) and a working volume of 1 l was used ...
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bioRxiv - Molecular Biology 2023Quote: ... The monodisperse peak of the complex was collected and concentrated with a 50 kDa cut-off concentrator (Vivaspin 2, Sartorius) and subsequently used for crystallization in LCP.
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bioRxiv - Physiology 2024Quote: ... Cultures were monitored for colony formation from 2 to 8 weeks after plating using Incucyte live cell imaging system (Sartorius). Wells with single colonies were harvested and cell cultures were expanded for making stocks and for DNA isolation ...
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bioRxiv - Bioengineering 2019Quote: ... 3 kDa MWCO concentrator (Sartorius), and the protein concentration was estimated using a BCA assay.
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bioRxiv - Bioengineering 2019Quote: ... 3 kDa MWCO concentrator (Sartorius). Protein concentrations were estimated by BCA assay.
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). Dose-response neutralization curves were analyzed by non-linear regression with a variable slope (GraphPadPrism v8 ...
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bioRxiv - Microbiology 2020Quote: ... 1% (w/v) polyvinylpyrrolidone and 2% (w/v) sodium dodecyl sulfate after which 200 mg glass beads (0.11 mm Sartorius, Gottingen, Germany) were added and the cells were lysed for 5 min at 2000 rpm in a FastPrep VR-96 instrument (MP Biomedicals ...
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bioRxiv - Immunology 2020Quote: ... and S309 for binding to soluble SARS-CoV-2 S trimer was assessed using the ForteBio Octet HTX (Sartorius Bioanalytical Instruments). All reagents were diluted to 100 nM in PBSF ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 10 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identificationbioRxiv - Cell Biology 2019Quote: ... 50 µl media with 2 x compound concentration was added and plates were subsequently placed and monitored in an IncuCyte® (Sartorius) instrument ...
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bioRxiv - Microbiology 2020Quote: Batch and fed-batch bioreactor experiments were performed in 2 L bench top glass fermentors (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed with 2% paraformaldehyde and % GFP positive cells were determined using flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG). Infection was normalized to conditions without antibody ...
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bioRxiv - Microbiology 2023Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). For experiments using single dilutions of serum or ExpiCHO-S cell supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... as well as phase) were taken every 2 h over a period of 72 h using the IncuCyte® (Sartorius, Germany). General macroautophagy flux was calculated via the ratio of the total integrated fluorescence intensities of GFP/RFP ...
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bioRxiv - Plant Biology 2024Quote: ... Fractions were pooled and concentrated to a final concentration of 2 mg/ml using a centrifugal filter device (Sartorius, Göttingen, Germany) with a molecular weight cut-off of 50 kDa.
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with a Vivaspin 6 (10 kDa MWCO) (Sartorius), flash-frozen by liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: Batch cultivations were performed in biological replicates (between 2-7 per condition) in 1.0 L Biostat Qplus bioreactors (Sartorius Stedim Biotech, Germany). The temperature was controlled at 28°C and pH was maintained at 6.0 (when desired ...