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Citations for Sartorius :
151 - 200 of 435 citations for 6 CHLORO 1 2 4 TRIAZOLO 4 3 B PYRIDAZINE 3 THIOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Lysates were spun at 15,000g for 15 min at 4°C and supernatants were then passed through a regenerated cellulose filter (4mm Ø, Sartorius). Samples were then dried under N2 flow at 40°C and resuspended in 125µl of ice-cold methanol/water 70:30 for subsequent analyses.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The resulting peptide solutions were centrifuged at 13,000 rpm for 15 min at 4 °C on tubes equipped with 2000 MW filtrate tubes (Sartorius, USA). Then ...
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bioRxiv - Biophysics 2020Quote: ... MgCl2 and EGTA were removed by 4 times buffer exchange using a Vivaspin 500.5 molecular weight cut-off (Sartorius, Göttingen). Samples were loaded onto a SDS-PAGE gel to confirm phosphorylation.
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bioRxiv - Cancer Biology 2022Quote: ... Cell proliferation was recorded for up to 7 days every 4 hours by the IncuCyte S3 Live-Cell Analysis System (Sartorius) with a phase-contrast microscope at 10X magnification ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing pure Fab16 were collected and concentrated to approximately 14 mg/ml using a VivaSpin 4 concentrator (10 kDa MWCO, Sartorius). Fab16 was crystallized by vapor diffusion at 4 °C by dispensing 200 nl of protein and 200 nl of crystallization buffer containing 1.5 M malic acid (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... cells and media were collected and this mixture was centrifuged at 3700xg at 4°C to remove debris and then concentrated by ultrafiltration using Vivaflow 200 (Sartorius). The purified AAV particles were collected after running the viral concentrate through an iodixanol column (Opti-Prep density gradient medium ...
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bioRxiv - Immunology 2021Quote: ... The purified Spike and RBD recombinant proteins were then buffer exchanged into sterile PBS by centrifuging at 4000xg for 30 minutes at 4 °C in a 10,000 MWCO Vivaspin centrifugal concentrator (Sartorius, Germany) and stored at –80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Cell Biology 2023Quote: MuSCs were freshly isolated and seeded on 0.2% gelatin-coated 96 well plates at a density of 2,000 cells per well in growing conditions and incubated for 4 days in an IncuCyte SX5 Live-Cell Analysis System (Sartorius) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... Protein was eluted using similar buffer with 300 mM imidazole and concentrated by centrifugation with 4 kDa (AlgF) or 30 kDa (AlgJ and AlgX) cutoff Vivaspin Turbo centrifugal concentrators (Sartorius). His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... IncuCyte® Annexin V Green Reagent was added directly in the medium at the dilution 1:200 and B cells were imaged every hour for 3.5 days using IncuCyte® Live-Cell Analysis System (Sartorius).
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Microbiology 2022Quote: ... The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm, Sartorius Stedim Biotech GmbH). The biomass was washed on the filter with 10 mL of 60% methanol and stored at -70 °C until metabolite extraction.
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Cancer Biology 2022Quote: ... Medium containing DMSO or increasing concentration of test compound was added to each well and spheroid growth was monitored for 4 days post-treatment using the IncuCyte® Spheroid Analysis system (Sartorius). Images from 4 days post-treatment and day 0 were analyzed using the IncuCyte® Spheroid Analysis software module ...
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bioRxiv - Microbiology 2019Quote: ... viruses were concentrated by centrifugation at 4 °C using vivaspin 20 concentrators with a molecular weight cutoff of 100K (Sartorius, Göttingen, Germany). Thereafter ...
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bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
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bioRxiv - Microbiology 2020Quote: ... liquid phage suspension was centrifuged at 4000 g for 10 min at 4°C and the supernatant was subsequently filter sterilised using a 0.45 μm pore sized membrane filter (Sartorius Minisart®, 16555). The filtrate phage lysate was stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: The purified proteins were concentrated and exchanged to 10 mM sodium citrate buffer (pH 5.0) using Vivaspin® Turbo 4 ultrafiltration units with a 5000 MWCO PES membrane (Sartorius; Cat.# VS04T11). The purity and concentration of each PcaLOOL was assessed by SDS-PAGE and the Pierce™ BCA Protein Assay Kit ...
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bioRxiv - Physiology 2019Quote: ... were inoculated with 2 mL of stock culture and grown at 30°C and at 200 rpm for 8 h in a B Braun Certomat BS-1 incubator (Sartorius, Melsungen, Germany). Bioreactor batch cultures were started by transferring 400 mL of preculture to a bioreactor containing 4.6 L of medium ...
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bioRxiv - Plant Biology 2019Quote: ... These filtrates were ultra-filtered at 4°C through 20-ml Vivaspin micro-concentrators (10,000 molecular weight cut off, Sartorius Stedim, Biotech GmbH, Germany). Retained protein suspensions were used for protein content and enzyme assays ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Cancer Biology 2023Quote: Real-time calcein intensity was monitored using fluorescence live-cell imaging combined with a phase-contrast setup using the 4×objective (Sartorius AG, Gottingen, Germany). Images were acquired hourly and analyzed using Incucyte software and ImageJ.
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bioRxiv - Microbiology 2021Quote: ... The samples were weight with scales from KERN ALS 220-4 (KERN & SOHN GmbH, Balingen-Frommern, Germany) and Sartorius Cubis®-series (Sartorius AG, Göttingen, Germany). During the extraction process ...
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bioRxiv - Physiology 2023Quote: ... Bundles were lyophilized in a freeze-dryer (Labconco, Kansas City, MO, USA) for >4 h and weighed on a microbalance (Sartorius Cubis Microbalance, Gottingen, Germany). The rate of mH2O2 emission was calculated using a standard curve under the same assay conditions and then normalized to fibre bundle dry weight.
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bioRxiv - Microbiology 2021Quote: ... putida KT2440 was cultivated in a stirred bioreactor (Biostat B-Twin, Sartorius) in continuous mode with glucose feed pulses (0.5 g L-1 per pulse ...
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bioRxiv - Microbiology 2023Quote: ... putida KT2440 was grown in a stirred bioreactor (Biostat B-Twin, Sartorius) with a total volume of 2 L and a working volume of 1 L ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... After concentration (Vivaspin 6 Concentrator, Sartorius Stedim Biotech) and desalting (PD 10 Desalting Columns ...
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-MC and ES5838 (4 x 104) cells per well were plated in a 24-well plate and placed in an IncucyteS3 incubator (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Nine images per well were taken every 6 hrs for seven days ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with a Vivaspin 6 (10 kDa MWCO) (Sartorius), flash-frozen by liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a slow filter paper (#293; 1–2 µm Sartorius, Thermo Fisher Scientific), and then used to determine electrical conductivity (EC) ...
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bioRxiv - Systems Biology 2022Quote: Co-culture experiments were performed in lab-scale stirred bioreactors (Biostat B-Twin, Sartorius). The processes were performed with a working volume of 1L at a constant temperature of 30°C ...
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bioRxiv - Neuroscience 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Synthetic Biology 2019Quote: pH-Controlled bioreactor batch fermentations were conducted with a Biostat B+ (Sartorius Stedim, NY, USA) dual 1.5 L fermentation system at a working volume of 1 L M9 hybrid medium ...
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bioRxiv - Microbiology 2021Quote: Cultures in segregostat mode were performed in lab-scale stirred bioreactor (Biostat B-Twin, Sartorius) with 1 L working volume ...
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bioRxiv - Bioengineering 2024Quote: The three-step batch cocultivation was done by using Biostat B Plus bioreactor (Sartorius, Germany) equipped with pH-meter (Hamilton ...
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bioRxiv - Microbiology 2020Quote: ... using the Vivaspin 6 centrifugal concentrator (MWCO 1,000,000 kDa) (Sartorius, Australia), was required for high-quality electron microscopy visualization ...
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). The purified and concentrated protein was delivered to GeneScript-Nanjing to raise the anti-rabbit polyclonal antibody.
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). Concentrations of the purified proteins were measured using BCA protein assay kit (Beyotime ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluted complex was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius), and imidazole was removed from the protein solution by applying the sample to a PD MiniTrap Sample Column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... thermolithotrophicus was continuously grown in a 10 L fermenter (BIOSTAT® B plus, Sartorius, Göttingen, Germany) under diazotrophic conditions with either 10 mM Na2SO3 or Na2SO4 as sulfur source instead of Na2S ...
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bioRxiv - Microbiology 2024Quote: ... A global association 1:2 bivalent analyte model using the Octet Data Analysis HT software v7 (Sartorius) was used to account for the dimeric HAstV-VA1 spike ...
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bioRxiv - Cell Biology 2021Quote: ... concentrated to 300-350 µl using Vivaspin 6 30,000 MWCO concentrators (Sartorius), and loaded onto a Superdex 200 10/300 GL column equilibrated in SEC Buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80°C before use.
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bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80 °C before use.
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bioRxiv - Biochemistry 2023Quote: ... Dialysed protein was spin-concentrated in a Vivaspin 6 MWCO 50,000 (Sartorius) to the desired concentration ...
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bioRxiv - Systems Biology 2023Quote: ... followed by live-cell imaging every 6 h using Incucyte Systems (Sartorius) for the duration of 1 week ...
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bioRxiv - Systems Biology 2021Quote: ... faecalis V583 ΔglnA was grown in glucose-limited chemostat cultures in Biostat B Plus benchtop bioreactors (Sartorius) in 750 ml CDM-LAB with a dilution rate of 0.15/h at 37 °C and gassing with 0.05 L/min nitrogen and stirring with 250 rpm ...