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Citations for Sartorius :
101 - 150 of 316 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Ubiquitylated material eluted from biotin pulldown assays was concentrated in a Vivaspin 500 centrifugal filter units (Sartorius) and resolved by SDS-PAGE in 4–12% Bolt Bis-Tris precast gels (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... then 100 mL of the appropriately diluted sample was filtered through a cellulose nitrate membrane filter with a pore size of 0.45 μm (Sartorius, Goettingen, Germany) using a vacuum pump ...
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bioRxiv - Plant Biology 2020Quote: ... overlain by a 0.8 mm thick diffusive gel disc and a protective membrane disc (polyethersulfone, 0.45 μm pore size, 0.15 mm thick, Sartorius Stedim, Göttingen, DE), encased by a plastic sampler housing (see Zhang & Davison (1995 ...
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bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
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bioRxiv - Biochemistry 2024Quote: ... adding 3 ml of ice-cold Tris buffer and by filtrating the PLs through 0.45 μm pore-size membrane filters (Sartorius Stedim Biotech). Filters were washed twice with 3 ml of Tris buffer and allowed to dry ...
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bioRxiv - Microbiology 2023Quote: ... were used to collect air on 37 mm sterile gelatin filters (3 µm pore size; Sartorius, Cat. No. 12602-37-ALK) containing a backing filter to ensure the gelatin filter remained intact during sampling ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 cells with nuclear-localized GFP (4T1-nucGFP) were established by introducing NucLight Green Lentivirus Reagent (Essen BioScience by Sartorius, Goettingen, Germany) under the selection of 2 µg/ml puromycin ...
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bioRxiv - Immunology 2022Quote: Binding kinetics of WS6 IgG and related S2-stem helix antibodies to diverse CoV 2P-stabilized spike proteins (S2Ps) were measured by biolayer interferometry using an Octet HTX instrument (Sartorius). All assays were performed in tilted 384-well plates (Geiger Bio-one ...
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bioRxiv - Microbiology 2021Quote: ... For virome sequencing they were filtered through a 0.45 μm pore size PES filter (Minisart® High Flow Syringe Filter, Sartorius, Göttingen, Germany). For the metagenome sequencing DNA was extracted using the method of Mortensen et al [42] and the DNA was sequenced ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl spots of serial dilutions of late-exponential phase cultures were placed on 0.45 μm pore size filters (Sartorius Stedim Biotech GmbH) and they were left to air-dry in a laminar flow cabin for 5 h (5 min in the control assay) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant consisted of purified FF which was sterile-filtered using a 0.22 μm pore size membrane filter (Minisart, Sartorius Stedim Biotech S.A.), alicuoted and stored at −80°C ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 100 mL of rainwater was collected per Schott-bottle and filtered through a cellulose acetate filter (25 mm in diameter, 0.8 μm pore size, Sartorius-Stedim, Goettingen, Germany) in polycarbonate filter housings ...
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bioRxiv - Microbiology 2020Quote: ... liquid phage suspension was centrifuged at 4000 g for 10 min at 4°C and the supernatant was subsequently filter sterilised using a 0.45 μm pore sized membrane filter (Sartorius Minisart®, 16555). The filtrate phage lysate was stored at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... The protein elutes were concentrated using a protein concentrator (Sartorius #VS15T01). For protein labeling ...
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bioRxiv - Microbiology 2020Quote: ... collected from each sampling point in the mesocosm experiment was filtered through 0.2 µM pore cellulose acetate membrane filter (Sartorius Stedim Biotech, Göttingen, Germany). Nucleic acid was subsequently extracted from each of the filtered samples using the PowerWater DNA Isolation Kit (MoBio ...
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bioRxiv - Developmental Biology 2022Quote: ... EpiLC differentiation medium was added and supplemented with 5µM of Caspase 3/7 Green dye (Sartorius) to detect for apoptosis in live cells ...
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bioRxiv - Biochemistry 2022Quote: ... followed by the removal of free dye through 10 kDa Vivaspin® 500 centrifugal concentrators (Sartorius Stedim Biotech ...
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bioRxiv - Microbiology 2022Quote: The affinity between each antibody and spike protein was measured by bio-layer interferometry (BLI) with a BLItz Biolayer Interferometer System (Sartorius, Goettingen, Germany).
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bioRxiv - Biophysics 2024Quote: ... The purified proteins were then concentrated using a protein concentrator (Sartorius #VS15T01) and verified by SDS-PAGE (Fig ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μM of small-molecule targets with biotin conjugated were used as ligands to be immobilized to the Octet Streptavidin biosensors (Sartorius). Titration experiments were performed in 25C ...
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bioRxiv - Biochemistry 2024Quote: ... A 20-kDa fragment of HA with a single biotin molecule per chain (Creative PEGWorks, 250 nM) was immobilized onto streptavidin tips (Sartorius). These tips were then incubated with purified ACAN proteins at a series of concentrations for 180 s during the association phase ...
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bioRxiv - Microbiology 2024Quote: ... protein A biosensors (Sartorius) were first loaded with 10 μg/mL of mAb/bsAb in the running buffer until a binding threshold of 1 nm was reached ...
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bioRxiv - Microbiology 2023Quote: ... Proteolytic DEVD cleaving activity was determined using the Incucyte Caspase-3/7 Red Dye for Apoptosis (Sartorius) as described in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then seeded in 96-well plates in cell growth media containing Annexin V Dye (Sartorius). Cells were imaged by real-time fluorescence microscopy (IncuCyte ...
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bioRxiv - Cancer Biology 2023Quote: ... of PD-L1 protein/PD-L1 antibody was determined by Octet Biolayer interferometry (BLI) using the Octet RED384 system (Sartorius, Bohemia, NY, USA). Briefly ...
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bioRxiv - Microbiology 2024Quote: Protein–protein interactions were measured using the Octet N1 system (Sartorius, Göttingen, Germany). His-SeFtsZ and His-SeSepF were diluted in BLI buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The Cy5-labeled tau proteins were then concentrated using a protein concentrator (Sartorius #VS15T01).
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in the presence of a green mortality probe (1/3000, Incucyte® Caspase-3/7 Green Dye - Sartorius, Germany). Green fluorescence was immediately followed every 90 or 120 minutes with an Incucyte® S3 Live-Cell Analysis system (Sartorius ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was changed to 90 µL of growth media containing 1:1000 Incucyte Nuclight Rapid Red Dye (Sartorius, 4717) and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated at 20,000 cells/ml in Geltrex-coated 96-well plates and treated with different doses of inhibitor in the presence of 1:1000 IncuCyte® Caspase-3/7 Dye for Apoptosis (Sartorius). Doubling times were calculated by determining cell counts over multiple days with the IncuCyte Live Cell Analysis System cell-by-cell module (Sartorius).
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bioRxiv - Cancer Biology 2024Quote: ... On the day of the assay tumour media was replaced with fresh media with Incucyte® Cytotox Green Dye (Sartorius, 4633), according to the manufacturer instructions and incubated for 10 min prior T cells addition ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...
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bioRxiv - Cancer Biology 2023Quote: 2,000 cells per well were plated in 96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network external controller hard drive that gathered and processed image data ...
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bioRxiv - Cancer Biology 2023Quote: Analysis of apoptosis using fluorescence live cell microscopy Cells were stained with Incucyte® Caspase-3/7 Dye for Apoptosis (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using Vivaspin centrifugal filters (Sartorius) of appropriate cut-offs ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100kDa MWCO Vivaspin protein concentrator (Sartorius), aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Vivaspin concentrators (Sartorius Stedin) and the sample purity and homogeneity was assessed by SDS-PAGE.
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bioRxiv - Cell Biology 2024Quote: ... the protein was concentrated using Vivaspin column (Sartorius). Pellets from KCMF1-Strep expression were resuspended in 50mM Hepes/KOH pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: The kinetics of the protein-protein interactions were determined using the bio-layer interferometry from the BLI system (Sartorius Octet BLI Discovery). Protein-protein interactions were observed by immobilizing 0.05 mg/ml of His-tagged VARP FL on a Ni-NTA biosensor or 0.05 mg/ml of biotinylated N-VARP on a streptavidin biosensor ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated using centrifugal concentrators (VivaSpin, Sartorius) and then assessed for successful labeling by measuring fluorescence using the following settings and the monochromator in the BioTek Synergy Neo2 plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT and proteins concentrated via centrifugation (Vivaspin, Sartorius). Recombinant kinase was added to 10µl of 2X kinase buffer [40mM Hepes (pH 7.5) ...