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Citations for Sartorius :
101 - 150 of 363 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were grown at 37°C and 5% CO2 in an Incucyte Zoom (Sartorius). Cell death was monitored hourly.
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bioRxiv - Immunology 2023Quote: ... Human antibodies (5 μg/mL or 7.5 µg/mL) were captured on pre-conditioned anti-human IgG Fc-capture biosensors (AHC, Sartorius) for 600 s ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius, Göttingen, Germany), analyzed by SDS-PAGE and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... followed by centrifugation of denatured proteins on a filter device (Sartorius, Vivacon 500). After several washing steps with 8 M urea and ammonium bicarbonate ...
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bioRxiv - Biochemistry 2021Quote: ... The immobilization of ligand proteins on amine reactive second-generation (AR2G) biosensors (Sartorius) was done following manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted to 100 nM in Kinetics buffer (Pall FortéBio/Sartorius). Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using ultrafiltration through a 30 kDa cutoff filter (Sartorius), snap-frozen and stored at −80 °C until further used.
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using ultrafiltration through a 50 kDa cutoff filter (Sartorius), snap-frozen and stored at −80 °C until further use ...
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bioRxiv - Synthetic Biology 2024Quote: ... and aqueous solutions containing protein components were filtered using Minisart syringe filters (Sartorius). 0.5x1mm (I.DxO.D. ...
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bioRxiv - Microbiology 2020Quote: ... Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius). The concentrate was then dialyzed using a dialysis tube (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diafiltered at 4°C using 3 kDa cutoff VivaSpin®500 centrifuge filters (Sartorius) for 3 cycles of 20 min at 14,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... which took place overnight on arrival at 4°C (cold room) using 6 Vivaflow200 filtration units (Sartorius) operating in two parallel series ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% nonfat dry milk) to 100 nM and immobilized for 60 s to anti-human IgG Fc capture biosensors (Sartorius). Sensors were transferred to assay buffer for 30 s to set the baseline ...
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bioRxiv - Biochemistry 2020Quote: Digestion of 10His-SUMO1T95R proteins was performed on 10 kDa-cutoff spin filters (Sartorius) according to a published protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the protein was simultaneously concentrated and buffer-exchanged (with a Vivaspin Turbo 4; Sartorius) into PM buffer (80 mM Pipes pH 6.8 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were filtered using Minisart 0.45 μm filter (Sartorius Stedim Biotech, Goettingen, Germany). Part of the extract was set aside prior to immunoprecipitation and used for SDS-PAGE as described above ...
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bioRxiv - Bioengineering 2023Quote: ... The AFC-eluates were buffer exchanged with 10kDa Vivaspin protein concentrator (Sartorius Stedim, Germany) using Sorval Lynx XTR centrifuge (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Biophysics 2023Quote: ... Proteins solutions were concentrated to at least 600 µM with Vivaspin centrifugal concentrators (Sartorius) and stored at -80 °C.
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and Ulla proteins were concentrated to 35 ∼ 50 mg/ml using a VIVASPIN20 (Sartorius) concentrator and stored at −80 °C until use.
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG M2 was immobilized on protein G biosensors (Sartorius Cat. No. 18-5082) at 5 µg/mL (in binding buffer ...
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bioRxiv - Biophysics 2021Quote: ... at pH 7 via centrifugal filter units at 12000 g at 4°C (Vivaspin 500, MWCO 10000, Sartorius). Glycans for native MS analysis were mixed with 1 µM (per monomer ...
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bioRxiv - Cell Biology 2022Quote: ... cells were placed in the Incucyte SX5 and maintained at 37°C and 5% CO2 (Sartorius, Göttingen, Germany) for detection and automated analysis of cell growth/proliferation ...
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bioRxiv - Synthetic Biology 2022Quote: ... Co-cultures were incubated at 37°C and imaged with an Incucyte S3 Live-Cell Analysis System (Sartorius) for approximately 72 hours ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Immunology 2022Quote: ... Purified protein was buffer exchanged into PBS and concentrated using a Vivaspin concentrator (Sartorius-Stedim) to 3-5 mg/mL and flash-frozen with liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2021Quote: ... Remaining dye was washed away by concentrating protein using Vivaspin® 500 centrifugal concentrators (Sartorius) into MST buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein complexes were concentrated to 25 µL in 30 kDa centrifugal concentrator (Vivaspin 500 Sartorius) and mixed with equal volume of 2 x native gel sample buffer (62.5 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was first concentrated by crossflow filtration using a Vivaflow 200 system (Sartorius) and two Hydrosart cassettes (MWCO ...
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bioRxiv - Biophysics 2022Quote: ... The protein was concentrated to the desired concentration using a Vivaspin Turbo centrifugal concentrator (Sartorius) with a 3 kDa membrane by centrifugation at 4000 g and 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Purified His6-MBP-tagged proteins were then concentrated with a 30,000 MWCO centrifugal concentrator (Sartorius) and buffer exchanged into 20 mM HEPES pH 7.5 and 50 mM NaCl for crystallization ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius, Göttingen, Germany). Proteins were purified to homogeneity using a size-exclusion chromatography column (HiLoad® Superdex ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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bioRxiv - Physiology 2023Quote: EV suspensions containing 50 µg protein were digested using FASP digestion (Vacacon500, Sartorius, VN01H02 10kDA), and sample volumes were adjusted to 100 µL with 8M urea ...
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bioRxiv - Biochemistry 2020Quote: ... oxidized BdiGolSase1 was ultrafiltrated by centrifuging at 10,000 x g and 4°C using a Vivaspin 500 device (Sartorius). To evaluate the reversibility of the process ...
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bioRxiv - Biophysics 2020Quote: ... 250 mM) at pH 7.5 and 4°C via centrifugal filter units (13000 x g, Vivaspin 500, MWCO 10000 (Sartorius) or Micro Bio-Spin 6 columns (Bio-Rad) ...
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bioRxiv - Bioengineering 2022Quote: ... the appropriate volume of stock solution was centrifuged at 3000g at 10 °C in Vivaspin® 20 concentrators (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated for 16 h at 37 °C following removal of RiGH26 using a Vivaspin 20 filtration unit (10.000 MWCO PES, Sartorius) and carbohydrate lyophilization on an ALPHA 2-4 LD Plus freeze dryer (Christ ...
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bioRxiv - Cell Biology 2019Quote: Live-cell imaging was performed at 37°C under humidified 5% CO2 atmosphere using the IncuCyte Zoom system (Sartorius). Phase and fluorescence images were also acquired using Leica DiM8 and Zeiss LSM710 microscopes ...
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bioRxiv - Molecular Biology 2022Quote: ... were concentrated to 2 ml by centrifugation 30 min at 4,000xg at 4°C through a 20 ml VivaSpin 20 30 kDa MWCO concentrator (Sartorius, and were diluted with TGE containing 1 mM β-mercaptoethanol to adjust the NaCl concentration to 50 mM ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 31.3 ng/mL for 45 minutes at 37°C in pHrodo Labeling Buffer (Sartorius), before being washed again and resuspended in complete RPMI medium ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were thawed and filtrated through a 0.2 µm filter before centrifugation at 4500 x g at 4°C in VivaSpin tubes (Sartorius). The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed once in PBS and kept in 500μL PBS at 4°C in the dark until analysis with an Intellicyt iQue3 Screener (Sartorius). For viability analysis ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 3 mL of Glutathione Sepharose 4B resin (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was incubated at 37°C and 5% CO2 and imaged using an Incucyte Live-Cell imager (Sartorius). The red channel used an acquisition time of 400 milliseconds for all replicates and samples ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 70,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 4 mL of IgG beads (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). Cleared lysates were then incubated with 1.5 mL of StrepTactin Sepharose beads (IBA Lifesciences ...