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Citations for Sartorius :
51 - 100 of 105 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Both batch and perfusion cultures were performed in a wave-mixed bioreactor (Biostat® RM, Sartorius) which maintained pH at 7.2 ± 0.1 through the addition of 1 M sodium bicarbonate or CO2 in the overlay gas mixture and pO2 at 60% ± 5% by addition of O2 to the overlay gas ...
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bioRxiv - Neuroscience 2020Quote: ... after which conditioned media was collected and concentrated 50-fold using 10 kDa cutoff concentrators (Sartorius #14558502). Protein concentration was measured using the Bradford assay ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed in PBS and grown for 48h in EBSS media (Sartorius cat# 02-010-1A). U2OS cells expressing GFP-G3BP (51 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then seeded in 96-well plates in cell growth media containing Annexin V Dye (Sartorius). Cells were imaged by real-time fluorescence microscopy (IncuCyte ...
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bioRxiv - Systems Biology 2021Quote: ... faecalis V583 ΔglnA was grown in glucose-limited chemostat cultures in Biostat B Plus benchtop bioreactors (Sartorius) in 750 ml CDM-LAB with a dilution rate of 0.15/h at 37 °C and gassing with 0.05 L/min nitrogen and stirring with 250 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... monoclonal hybridoma cell culture supernatant was concentrated using Vivacell 100 PES 30K MWCO concentrators (Sartorius, Fisher Scientific) and then bound to a HiTrap Protein G HP column (GE Healthcare) ...
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bioRxiv - Bioengineering 2023Quote: ... The serum-free suspended cells were cultured in microbioreactor (Ambr® 15 cell culture bioreactor; Sartorius, Germany) to determine the specific productivity (Qp ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell culture was inoculated at OD600 0.3 in the 2 L bioreactor (Biostat B, Sartorius, Germany) including the 1 L modified M9 minimal medium ...
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bioRxiv - Immunology 2022Quote: ... The media was concentrated to 500μL using the Vivaspin 15R Hydrasart 30,000 MWCO columns (Cat. No. FIL8452, Sartorius). This 500μL was loaded onto qEV Original (70nm pore ...
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bioRxiv - Cancer Biology 2023Quote: ... media was changed and live-cell timelapse microscopy of treated cells was performed in an Incucyte SX5 (Sartorius). Images were taken every 30 minutes in the phase ...
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bioRxiv - Bioengineering 2024Quote: ... Agent V treated conditioned media was clarified to remove cell debris by pumping through a depth filter (Sartorius). Pre-filter pressure was continuously monitored and maintained per the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2022Quote: ... The seed culture from each flask was transferred to a 1-L bioreactor (Biostat B-DCU, Sartorius, Germany) with 0.7 L fermentation medium ...
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bioRxiv - Synthetic Biology 2022Quote: ... Co-cultures were incubated at 37°C and imaged with an Incucyte S3 Live-Cell Analysis System (Sartorius) for approximately 72 hours ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were obtained by centrifugation (8,000 g, 10 min) and filtration (0.22 μm filters; Sartorius, Göttingen, Germany). Stx production was tested using Vero cell cytotoxicity assay (supplement) ...
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bioRxiv - Bioengineering 2024Quote: ... the shake flask culture was centrifuged for 5 min at 110 xg (Model 6K15, Sartorius AG, Göttingen, Germany). The supernatant was discarded and the cell pellet was resuspended in an appropriate volume of medium to generate a stock cell solution with an estimated concentration of 6.6 × 106 cells mL−1 for A and of 2.2 × 107 cells mL−1 for B ...
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bioRxiv - Biophysics 2022Quote: ... coli strain MG059e16 which harbors a His10>-tag after Gly-83 in the hycE gene of the hycABCDEFGHI operon was expressed in LB media supplemented with 0.4% glucose by anaerobic fermentation in a 200 L reactor (Sartorius). The culture was inoculated with 400 mL of aerobically grown preculture and stirred at 50 rpm for 16 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Then pre-cleared media was concentrated using Vivaflow® 50R cassette with 100 kDa molecular cut-off (VF05H4, Sartorius). Concentrated media was centrifugated at 100000×g for 4 h ...
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bioRxiv - Microbiology 2020Quote: Aerobic fermentor growth of ΔssaACB cell culture was achieved using a BIOSTAT® B bioreactor (Sartorius Stedim, Göttingen, Germany) and samples were collected as described in Puccio and Kitten (2020) ...
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bioRxiv - Microbiology 2020Quote: ... Samples (1 mL) from the liquid phase of the cultures were filtered (MiniSart RC4, 0.2 μm, Sartorius, Göttingen, Germany) and acidified with 2.5 μL H2SO4 ...
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bioRxiv - Biochemistry 2023Quote: The fed-batch bioreactor culture experiments were run using an AMBR250 modular bioreactor (Sartorius Stedim North America, Bohemia, NY) with a starting volume of 200 mL ...
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bioRxiv - Microbiology 2023Quote: ... Vesicles were purified from 1 L of culture per replicate using a tangential flow filtration system (Vivaflow 200, Sartorius). Details on all methods ...
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bioRxiv - Microbiology 2024Quote: ... These enrichment cultures were passed through a 0.22 µm filter to remove the remaining cells (Minisart High Flow PES, Sartorius), and the filtrate was used with the superhost in an agar overlay for viral plaque isolation as described above ...
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bioRxiv - Immunology 2021Quote: ... the thawed media was filtered and concentrated to 150-200 mL by tangential flow with a Vivaflow® 200 (Sartorius). The resulting sample was diluted with an equal volume of Buffer A ...
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Immunology 2022Quote: ... complete media was then layered on top for imaging with an Incucyte S3 live-cell analysis system (Sartorius, Göttingen, Germany). Imaging was performed every hour at 4X magnification using red ...
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bioRxiv - Molecular Biology 2022Quote: ... Conditioned media from hyperflasks was collected and concentrated in bulk using the Sartorius Vivaflow Cross-flow System (Sartorius, NY, USA) with Vivaflow 200 10,000 MWCO PES filters (Sartorius ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5 x 103 cells/well were plated in FBS-free media in the upper chamber of a 96-well Incucyte ClearView cell migration plate (8 µm pore size; #4582 Sartorius). Cells were incubated at 37°C for 45 minutes to allow cells to settle on the membrane before adding RPMI-1640 media supplemented with 10% FBS to the bottom chamber ...
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bioRxiv - Neuroscience 2023Quote: Mouse microglia and hiMGL conditioned media were digested using filter aided sample preparation (FASP) as previously described.22 For microglia lysates Vivacon spin filters (Sartorius) with 30 kDa cut-off were used ...
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bioRxiv - Cancer Biology 2023Quote: ... 7500 cells per genotype were seeded in 96-well plates in both media conditions and proliferation was monitored via the IncuCyte Zoom imaging platform (Sartorius) for up to 72 hours ...
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bioRxiv - Cell Biology 2022Quote: ... individual frames of cells growing under normal culture conditions were acquired every 5 minutes for 24 hours using an IncuCyte ZOOM live-cell analysis system (Sartorius) equipped with a 20 x objective.
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bioRxiv - Genetics 2021Quote: ... Neuronal migration was measured during a 70-hour period from day 40 by transferring cell culture plates to the IncuCyte Live Cell Analysis System (Sartorius). Cells were maintained at 37°C and 5% CO2 with 20X magnification phase contrast images taken of whole wells in every 2 hours for the analysis period ...
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bioRxiv - Cancer Biology 2022Quote: ... Basolateral compartments were filled with culture medium containing 10% FBS and plates were placed in an Incucyte S3 Imaging System (Sartorius). Images of the apical and basolateral sides of the porous membrane were captured every 4 hours over 3 days ...
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bioRxiv - Physiology 2024Quote: ... Cultures were monitored for colony formation from 2 to 8 weeks after plating using Incucyte live cell imaging system (Sartorius). Wells with single colonies were harvested and cell cultures were expanded for making stocks and for DNA isolation ...
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bioRxiv - Microbiology 2023Quote: The sterile filtered culture/fecal supernatant was concentrated using centrifugal filters Centrisart with a filter cut-off at 100 kDA (Sartorius) by centrifugation centrifuged at 1,500 x g at 4°C (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Biochemistry 2022Quote: The bioreactor cell culture experiments were conducted in a 3L glass bioreactor using Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 1.75L ...
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bioRxiv - Cancer Biology 2024Quote: ... FIREX-transduced cell lines (DLD1 and SW480) were subjected to daily observation through live-cell imaging in an Incucyte cell culture microscope (Sartorius).
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bioRxiv - Developmental Biology 2024Quote: ... and 100,000 cells were resuspended in growth factor-free CNCC early maintenance media and seeded in an IncuCyte ImageLock 96-well plate (Sartorius 4379) in triplicate and incubated for 48 hours at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Immunology 2021Quote: Supernatants of Expi cultures and monoclonal hybridoma populations were collected, concentrated (Amicon, UFC810008) and filtered through a 0.2 μm filter (Sartorius, 16534-K). Affinities were then measured on an Octet RED96e machine (FortéBio ...
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bioRxiv - Cancer Biology 2022Quote: 700 cancer and 1400 stellate cells were seeded in culture medium containing 1% FBS into the apical compartment of an Incucyte Clearview 96-well plate (Sartorius, 4582) at a 1:2 ratio ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... A total 75 ml culture supernatant was concentrated and exchanged with buffer W using 10 kD cutoff centrifugal filters (Sartorius, Germany). A 50 µl of Strep-Tactin®XT agarose beads were added to the concentrated sample in a final volume of 1 ml containing 1x protease inhibitor cocktail and incubated overnight at 4 ℃ ...
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bioRxiv - Microbiology 2022Quote: ... the pre-cultures were transferred with peristaltic pump into the 02 L (DCI-Biolafitte, US) and 20 L (Sartorius Bbraun, US) bioreactors respectively ...
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bioRxiv - Cancer Biology 2024Quote: The growth and viability of PDAC organoids and PBMCs organoid co-cultures were monitored with the live-cell imaging system IncucyteR S3 (Sartorius, Germany). The Incucyte S3 system from Sartorius proves to be a valuable asset for observing the time-lapse behavior of organoids in response to a particular treatment ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants were harvested and clarified by centrifugation (3000xg for 20 minutes) or using Sartoclear Dynamics® Lab V (Sartorius). Supernatants were then filtered at 0.2 μm using Nalgene Rapid Flow sterile single using vacuum filter units (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... All media and buffers were prepared using ultrapure water prepared by using an arium®pro ultrapure water purification system (Sartorius, Germany). Prior inoculation ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl spots of serial dilutions of late-exponential phase cultures were placed on 0.45 μm pore size filters (Sartorius Stedim Biotech GmbH) and they were left to air-dry in a laminar flow cabin for 5 h (5 min in the control assay) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cell dry weight (CDW) measurements were performed by filtrating 1 mL of cell culture through a weighed 0.45 μm filter membrane (Sartorius Biolab, Gottingen, Germany) and weighting the filter after microwaving it for 15 min and letting it dry in a desiccator for 24h.
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bioRxiv - Plant Biology 2022Quote: ... or sterilized water (as control) was then added over the root culture before covering it with a gas-permeable plastic film (bioFOLIE 25; Sartorius, Goettingen, Germany). Fungus-root interaction was monitored daily using a stereomicroscope and contact sites were imaged at 7 ...
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bioRxiv - Biochemistry 2023Quote: The perfusion cell culture experiments were conducted in a 5 L glass bioreactor using a Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 2 L ...
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bioRxiv - Synthetic Biology 2024Quote: Three cell lines were further characterised in a fed-batch culture was run in the ambr®15 system (Sartorius, Göttingen, Germany). Three bioreactors were inoculated for each cell line (CHO-S bcl-2 CHO ...