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Citations for Sartorius :
51 - 100 of 117 citations for Streptavidin ZAP Antibody Kit goat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... IgGs and Fabs were assessed for binding to biotinylated antigens using streptavidin (SA) biosensors (Sartorius/ForteBio) or to unbiotinylated ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% BSA) was used for 40 s to immobilize the Nb on streptavidin coated biosensor tips (SA, Sartorius). In the association step ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Immunology 2023Quote: ... 5 µg/ml of biotinylated hSIRPα diluted in Octet buffer (PBS, 0.1% BSA, 0.02% Tween20) was immobilized on streptavidin coated biosensor tips (SA, Sartorius) for 40 s ...
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bioRxiv - Immunology 2021Quote: ... biotinylated S1 binding mAbs at 25 µg/mL were first loaded on High Precision Streptavidin SAX biosensors (Sartorius Corporation) for 10 sec ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for binding using streptavidin (SA) biosensors (Sartorius/ForteBio) loaded to a threshold of 0.8nm of SARS-CoV- 2 ...
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bioRxiv - Microbiology 2022Quote: ... and BA.2+R493Q RBDs were diluted to 5 μg/mL and immobilized onto pre-hydrated streptavidin biosensors (Sartorius) to a level of 1 nm total shift ...
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bioRxiv - Neuroscience 2023Quote: ... we first loaded pre-equilibrated streptavidin biosensor probes with 400 nM of biotinylated peptides in 1X kinetics buffer (Sartorius) for 120 s ...
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bioRxiv - Bioengineering 2023Quote: ... to CD123 WT ECD and variants (captured ligands) was performed using Streptavidin capture biosensor (SA) (Sartorius, PN: 18-5019). CD123 WT ECD and variants were biotinylated using Biotinylation kit Type B (Abcam ...
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bioRxiv - Microbiology 2023Quote: Bio-layer interferometry analyses were conducted using a BLItz system equipped with High Precision Streptavidin (SAX) Biosensors (Sartorius, Germany). As a ligand ...
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bioRxiv - Biochemistry 2021Quote: ... Biotinylated-NIH-CoVnb-112 was diluted into assay buffer at 5 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated NPNA peptides were diluted to 5 μg/mL in kinetics buffer (KB) and immobilized onto streptavidin BLI biosensors (Sartorius); His-tagged rsCSP was diluted to ~1 μg/mL in KB and loaded onto Ni-NTA biosensors ...
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bioRxiv - Neuroscience 2022Quote: ... biotinylated VHH was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Immunology 2023Quote: ... two-fold serial dilutions of the biotinylated avi-tagged CH505 TF chSOSIPv4.1 and Man5GlcNAc2-enriched CH505 M5.G458Y chSOSIPv4.1 Env trimers were immobilized on hydrated Streptavidin (SA) Tips (Octet® Sartorius) for 120 seconds ...
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bioRxiv - Bioengineering 2023Quote: ... to CD123 WT ECD and variants (analytes in solution) was performed using Streptavidin capture biosensor (SA) (Sartorius, PN: 18-5019). CaptureSelect™ Biotin Anti-LC-kappa (Murine ...
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bioRxiv - Bioengineering 2023Quote: ... IL-2Rα and IL-2Rβ were immobilized to streptavidin-coated tips for analysis on an Octet Red96 BLI instrument (Sartorius). Less than 5 signal units (nm ...
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bioRxiv - Cancer Biology 2023Quote: ... The UGT2B15 promoter 20 base-pair sequence (5’-TAACTTGATTGATTTTTCCT-3’ for wild type and 5’-TAACTTGGCTGTCTTTTCCT-3’ for mutant) was immobilized to a streptavidin SADH sensor chip (Sartorius) in running buffer (10 mM HEPES pH 6.8 ...
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bioRxiv - Neuroscience 2023Quote: ... Octet® SAX high precision streptavidin biosensors and Octet® 384TW tilted-bottom plates were purchased from Sartorius (Göttingen, Germany). Peptides were synthesized by GenScript (Piscataway ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μM of small-molecule targets with biotin conjugated were used as ligands to be immobilized to the Octet Streptavidin biosensors (Sartorius). Titration experiments were performed in 25C ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
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bioRxiv - Biochemistry 2021Quote: A stock solution of sfGFP-ACE2 was diluted to at 10 μg/ml for immobilization onto High Precision Streptavidin (SAX) biosensors (Sartorius, Germany) in assay buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... A template with a biotin-TEG at the 5’-end was annealed to the primers (Table 2) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). Primers were added at two-fold molar excess over the template ...
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bioRxiv - Biochemistry 2022Quote: ... A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1) was prepared by ligation and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG) at 50 nM concentration for 7 min at 500 rpm ...
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bioRxiv - Biochemistry 2024Quote: ... The 35-mer template with a biotin-TEG at the 5’-overhang was annealed to an 11-mer primer (Supplementary Table 1) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). A chimeric RNA-DNA primer demonstrating the highest affinity to Polα (45 ...
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bioRxiv - Microbiology 2021Quote: ... A 5 μg/ml concentration of biotinylated stable O1/Manisa/TUR/69 FMD VLP (61) (kindly provided by Alison Burman) was immobilised on streptavidin-coated biosensors (Sartorius UK Limited) for 900 s ...
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bioRxiv - Biochemistry 2022Quote: ... In our BLI experiments, ACE2 Fc-fusions are tethered to either Streptavidin biosensors (WT-ACE2, CVD293) (Sartorius®, Item no.: 18-5019) or ProtA biosensors (Sartorius® ...
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bioRxiv - Immunology 2022Quote: Biotinylated OspCA (3 μg/mL) in buffer (PBS containing 2% w/v BSA) was captured onto streptavidin biosensors (#18-5019, Sartorius, Goettingen, Germany) for 5 min ...
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bioRxiv - Biochemistry 2024Quote: Binding kinetics of ZHER2-CK2α were measured by biolayer interferometry (BLI) on an OctetRed384 using in-house made HER2-Fc-AviTag immobilized on streptavidin biosensors (Sartorius, 18-5021). Kinetic measurements were performed in PBS with 0.1% bovine serum albumin and 0.05% Tween 20.
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bioRxiv - Immunology 2022Quote: ... GAGs had biotin attached at their reducing end with a previously described approach (35) before immobilisation to High Precision Streptavidin (SAX) biosensors (Sartorius AG, Goettingen, Germany). To achieve this SAX biosensors were hydrated for 10 mins in assay buffer (10 mM Hepes ...
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bioRxiv - Immunology 2022Quote: ... GAGs were biotinylated at their reducing end using an approach described elsewhere (44) and immobilised onto High Precision Streptavidin (SAX) biosensors (Sartorius AG, Goettingen, Germany). To immobilise ...
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bioRxiv - Cancer Biology 2024Quote: ... Mitochondrial membrane potential kit (Sartorius, 4775) was used for MMP measurements according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
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bioRxiv - Cell Biology 2023Quote: The XTT cell proliferation kit (BioInd, SARTORIUS) was performed according to the manufactural protocol ...
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bioRxiv - Cancer Biology 2024Quote: Internalization was assessed using the Incucyte Fabfluor-pH Antibody labeling reagent (Sartorius, Germany), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The purified antibodies were buffer exchanged to PBS using 100 kDa VivaSpin20 columns (Sartorius). The IgG concentration was determined on the NanoDrop 2000 and the antibodies were stored at 4°C until further analyses.
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bioRxiv - Cancer Biology 2023Quote: ... The XTT-based cell proliferation kit (Sartorius, Beit Haemek, Israel) was used to quantitate viability according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: BLI experiments on the antagonistic antibodies were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (PBS ...
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bioRxiv - Molecular Biology 2023Quote: The MultiCyt® QBeads® PlexScreen Secreted Protein Assay Kit (Sartorius) was used to quantify the level of tumor necrosis factor alpha (TNF-a ...
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bioRxiv - Immunology 2022Quote: The binding affinity of obtained antibodies to RBD was examined by the Blitz system (Sartorius Japan) using protein A-coated biosensors ...
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bioRxiv - Synthetic Biology 2022Quote: The binding kinetics of antibodies to RBD was measured by BLI on an Octet-R2 (Sartorius). The his-tagged RBD variants were loaded onto Ni-NTA biosensors from 10 ug\ml solution in kinetic buffer (PBS ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
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bioRxiv - Immunology 2024Quote: ... Supernatants were quantitated for antibody concentrations by bio-layer interferometry (BLI) on an Octet Red 96 (Sartorius) using protein A biosensors and a human IgG1 isotype control antibody as a standard ...
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bioRxiv - Immunology 2024Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
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bioRxiv - Molecular Biology 2023Quote: Antibody binding to Henipavirus F proteins was assessed using BLI on an Octet RED 384 (Sartorius, formerly ForteBio) with Forte Bio Kinetics Buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
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bioRxiv - Immunology 2024Quote: ... Antibodies were diluted to a final concentration of 20 ug/mL before being immobilized on ProteinA tips (Sartorius) for 600 s ...
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bioRxiv - Immunology 2021Quote: Affinity measurements for VHH-huFc antibodies were performed using biolayer interferometry (BLI) using an Octet 384 Red system (Sartorius). Measurements were conducted in 10 mM phosphate (pH 7.4) ...
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bioRxiv - Immunology 2023Quote: The binding affinity of recombinant antibodies to antigens was estimated by bio-layer interferometry (Octet® QK, ForteBio/Sartorius). Antibodies were loaded at 10-20 nM onto anti-human IgG Fc capture or anti-Mouse IgG Fc capture biosensors ...
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bioRxiv - Immunology 2024Quote: Analysis of serum antibodies binding to PNP scaffolds by biolayer interferometry (BLI) was performed on an Octet R4 (Sartorius) at 30 °C in kinetics buffer (PBS ...