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Citations for Sartorius :
51 - 100 of 109 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Using Vivaspin 500 columns with 30 or 10 kDa molecular weight cut off (Sartorius Stedim Biotech), the buffer was exchanged to 10 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing Mini-I were pooled and concentrated by ultrafiltration (Vivaspin 20, 30 kDa, Sartorius) before injection in a HiLoad 10/300 Superdex S200 Increase gel filtration column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting pool was concentrated to 900 μl with a 30-kDa cutoff concentrator (Sartorius, Germany). The sample was injected thrice on a Superose™ 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM DTT and concentrated to 200 μl with a 30-kDa cutoff concentrator (Sartorius, Germany). The protein concentration was determined via Bradford assay ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was pooled and concentrated with a Vivaspin 30 kDa molecular weight cut-off centricon (Sartorius) and loaded onto a buffer A equilibrated S6 Increase 3.2/300 (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... imidazole was removed using a 10 or 30 kDa cut-off centrifugal concentrator (Sartorius, Goettingen, Germany). The concentrated pure Saci_1123 and Saci_0315 proteins were stored in 25 mM PIPES-HCl (pH 6.5) ...
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bioRxiv - Neuroscience 2021Quote: ... The samples have then been loaded onto a standard filtration device (30 kDa MWCO; VivaCon 500, Sartorius) and centrifuged at 14’000 x g for 15 minutes at 20°C ...
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bioRxiv - Physiology 2020Quote: ... The remaining solutions were subjected to spin column purification (Sartorius Vivaspin 500 30 kDa MWCO PES, #VS0122) for three times by adding 500 μL of activity buffer for each cycle ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing Ldh-EtfAB were pooled and concentrated via ultrafiltration (Vivaspin 6, 30 kDa Cut-off, Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2020Quote: ... A large viral stock was prepared by pooling 30 mL of filtered supernatant (0.45 μM filter, Sartorius) from the samples harvested at days 2 and 3 from both second passages (total of 120 mL) ...
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bioRxiv - Microbiology 2020Quote: ... Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius). The concentrate was then dialyzed using a dialysis tube (Millipore ...
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bioRxiv - Evolutionary Biology 2024Quote: ... phages were recovered after centrifugation (30 min at 3,000 rcf) and filtration of the supernatant (0.22µm; PES #16541-K, Sartorius). Phages coming from co-culture with SeeT17 transformed with pBBR1-E ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting sample was subject to buffer exchange in 30 kDa MWCO Vivaspin Turbo centrifugal filters (Sartorius) against 25 mM HEPES/NaOH ...
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Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensorsbioRxiv - Bioengineering 2020Quote: ... Phase contrast images were taken at 30-minute intervals using an IncuCyte® Live-Cell Analysis System (Sartorius) in a humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... The cell debris was removed through centrifugation at 28,000 g for 30 minutes and the supernatant was filtered through a 0.45 µm sterile filter (Sartorius). The protein was then loaded into a 1-mL HisTrap column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... before concentration on a tangential flow filtration system equipped with a 30 kDa cut-off filter membrane (Sartorius). The ~200 mL retentate was equilibrated to 20 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... into 20 mM Tris / 500 mM NaCl / pH 8.3 using Sartocon ECO (30 kDa MWCO, 0.70 m²) PES membranes (Sartorius). Buffer exchanged supernatants were filtered using 0.8 + 0.45 µm Sartopore 2 filters (Sartorius ...
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bioRxiv - Molecular Biology 2021Quote: ... to 0.1 M Glycine / 10 mM Tris / pH 7.5 using a Sartocon Slice ECO (30 kDa MWCO, 0.14 m²) PES membrane (Sartorius). Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA ...
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bioRxiv - Molecular Biology 2021Quote: ... injection grade (≥ 10 volume changes) using a Sartocon Slice ECO (30 kDa MWCO, 0.14 m²) PES membrane (Sartorius). Buffer exchanged DS preparations were further adjusted to a concentration of ∼1 mg x mL-1 in 20 mM Tris / 150 mM NaCl / pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns (molecular weight cut-off of 30 kDa; Sartorius) and centrifuged at 4,000 x g at 4 °C until the supernatant was 100-fold concentrated ...
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2020Quote: ... and 1X EDTA-free protease inhibitor cocktail] was homogenized by 30-40 strokes in a glass Dounce homogenizer (Sartorius). The cell homogenate was cleared by centrifugation at 1,000xg twice ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 by repeated ultracentrifugation using a Vivaspin 500 filter device with a cutoff of 30 kDa (Sartorius) at 4 °C and afterwards concentrated ...
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bioRxiv - Cell Biology 2023Quote: ... samples were denatured in UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and centrifuged on a 30 kDa cut-off filter device (PALL or Sartorius) and washed thrice with UA buffer and twice with 50 mM ammoniumbicarbonate (ABC) ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were resuspended in 200 μL 8 M urea (pH 8.0) and loaded in 30 kD Microcon filter tubes (Sartorius) and centrifuged at 12,000 g for 20 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR was initially concentrated to a final concentration of 172 μM via centrifugal ultrafiltration (30-kDa molecular weight cutoff; Sartorius) and then extensively dialyzed against buffer C ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing pure agrin constructs as assessed by SDS-PAGE analysis underwent concentration through a Vivaspin Turbo 30 kDa MWCO concentrator (Sartorius) to reach a final volume below 0.5 mL ...
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bioRxiv - Biochemistry 2020Quote: ... 5% ethylene glycol), followed by buffer exchange to (30 mM Tris/HCl, pH 8.0) with Vivaspin centrifugal concentrator (Sartorius, Germany). The final phosphoprotein concentrations were adjusted to 1.5 mg/ml (80 μM) ...
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bioRxiv - Biochemistry 2020Quote: ... glycerol was added and the protein concentrated to 20 – 30 mg/mL in a 10 kDa MWCO spin concentrator (Vivaspin, Sartorius).
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bioRxiv - Biochemistry 2021Quote: The dialysed proteins were concentrated to a <5 mL volume using a 30 kDa MWCO Vivaspin 20 centrifugal concentrator (Sartorius) at 5000 g ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were analyzed for pure proteins by 12% SDS-PAGE and fractions containing pure enzymes were pooled and concentrated using Vivaspin concentrators with 30 kDa cut-off filter (Sartorius). Protein concentrations were determined using the corresponding extinction coefficient at 280 nm (ExPASy ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclear suspension was centrifugated for 30 min at 15 000 g and supernatant was concentrated using Vivaspin 6 (10 kDa MWCO) centrifugal concentrator (Sartorius). Aliquots of concentrated nuclear extracts were frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Microbiology 2021Quote: ... 120- and 180-minutes post aerosolization by passing air at 6L/min for 30 seconds from the drum through a 47mm gelatin filter (Sartorius). Filters were dissolved in 10 mL of DMEM containing 10% FBS and stored at -80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP-NANOG MDA-MB-231 cells were plated on fibronectin-coated (30 μg/mL) glass 96 well plates and placed in an Incucyte S3 (Sartorius) live cell imaging system ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Microbiology 2020Quote: ... The virus containing medium was concentrated down to approximately 30 mL using a pump-powered filter (100-kDa cut-off; Vivaflow, Sartorius) and then dialysed into an excess of buffer (10 mM Tris pH 8.0-150 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... The purified Spike and RBD recombinant proteins were then buffer exchanged into sterile PBS by centrifuging at 4000xg for 30 minutes at 4 °C in a 10,000 MWCO Vivaspin centrifugal concentrator (Sartorius, Germany) and stored at –80 °C ...
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bioRxiv - Microbiology 2023Quote: ... Final separation was achieved following concentrating the Heparin elution to approximately 8 mg/mL using a 30 kDa MWCO Vivaspin filter (Sartorius), by applying the sample to a Superdex 200 10/300 GL (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... Protein was eluted using similar buffer with 300 mM imidazole and concentrated by centrifugation with 4 kDa (AlgF) or 30 kDa (AlgJ and AlgX) cutoff Vivaspin Turbo centrifugal concentrators (Sartorius). His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... These clones were incubated until 10-30% confluency and screened for high GFP and RFP signal using Incucyte S3 (Sartorius). The highest expressing monoclonal cells were selected and further expanded on 6 well plates and again screened for fluorescence using the Incucyte ...
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bioRxiv - Cell Biology 2023Quote: ... Cell-free supernatants were concentrated to 1 mL by ultrafiltration through a membrane with a molecular weight cut-off of 30 kDa (VivaSpin 20, 30,000 MWCO PES (Sartorius, VS2022)) at 4 °C and 4,000 ˗ g ...
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bioRxiv - Microbiology 2022Quote: ... The fractions containing the sample of interest were pooled and concentrated using 10 kDa (MtPAPP) and 30 kDa cut-off centrifugal concentrator (Sartorius). The yield of this purification was ...
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bioRxiv - Biophysics 2023Quote: Nanodisc samples were concentrated to ∼200 μM in 280 μL in a Vivaspin-6 concentrator with a 30 kDa MWCO (Sartorius). 20 μL D2O was added and gently mixed into the sample ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were pelleted by centrifugation at 5250 × g for 30 minutes and the supernatant was collected and filtered through a 0.22 μm PES membrane (Sartorius®). The BsAbs were purified from the supernatant utilising a 5 mL HisTrap™ excel column (Cytiva™) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting clarified supernatant was loaded onto Vivaspin protein concentrator columns with a 30 kDa molecular weight cut-off (Sartorius) and centrifuged at 4,000 x g and 4 °C until the sample was concentrated by a factor of 100 ...
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bioRxiv - Microbiology 2024Quote: ... cells were irradiated (30 Gy) and co-cultured with JET cells [33] and placed in an IncuCyte live cell S3 analysis system (Sartorius). The cells were then continuously imaged for RFP every 3 hrs for 5 days ...
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bioRxiv - Neuroscience 2022Quote: ... A protein amount of 30 µg per sample for cell lysates and 10 µg for EV lysates was subjected to proteolytic digestion using the filter aided sample preparation (FASP) protocol (86) with 30 kD Vivacon spin filters (Sartorius, Germany). Proteolytic peptides were desalted by stop and go extraction (STAGE ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG) and passed through a spin-out column of Bio-Gel P-6 equilibrated with the protein storage buffer (0.1 M Hepes-HCl ...