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Citations for Sartorius :
51 - 100 of 106 citations for PEG NH2 modified upconverting red light since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The affinity measurement was performed on the ForteBio Octet RED 96 system (Sartorius, Goettingen, Germany). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... then labeled with the pHrodo Red Cell Labeling Kit for Incucyte (4649; Sartorius, Bohemia, NY) with a final concentration of 125 ng/mL dye following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Unique nanobodies were screened by biolayer interferometry (BLI) in an Octet Red 384 system (Sartorius) using Streptavidin SA biosensors (Sartorius ...
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bioRxiv - Immunology 2022Quote: BLI experiments on the antagonistic antibodies were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (PBS ...
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bioRxiv - Cancer Biology 2024Quote: All cell lines were stably transduced with Incucyte Nuclight Red Lentivirus (EF1α, puro, Sartorius #4476) and selected in 2 µg/ml puromycin for stable expression of nuclear-restricted red fluorescent label ...
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bioRxiv - Biochemistry 2023Quote: ... The optical interference pattern of white light was used to measure binding kinetics with the Octet® BLI Label-Free Detection System (Sartorius). MDA5 and LGP2 dissociation was measured by flowing buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... The affinity measurement was performed on the Forte bio Octet RED 96 system (Sartorius, Goettingen, Germany). Briefly ...
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bioRxiv - Immunology 2022Quote: ... and aged wild-type mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were stimulated with 5 μg/mL plate-bound αCD3 (Biolegend ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... cells were treated with Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling (Sartorius, 4717) at time of experiment seeding for a final concentration of 1:750 ...
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bioRxiv - Immunology 2022Quote: The Octet quantification assay for protein expression level was performed on an Octet 96 Red system (Sartorius). Harvested cell media were centrifuged and cell supernatants were prepared in a 96-well plate with S-2P or S-6P standards diluted in media from 20 μg/mL to 0.3125 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Proteolytic DEVD cleaving activity was determined using the Incucyte Caspase-3/7 Red Dye for Apoptosis (Sartorius) as described in the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were quantitated for antibody concentrations by bio-layer interferometry (BLI) on an Octet Red 96 (Sartorius) using protein A biosensors and a human IgG1 isotype control antibody as a standard ...
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bioRxiv - Immunology 2022Quote: BLI experiments on the IL-9 signaling complex were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2024Quote: Antibodies were incubated at a ratio of 1:3 antibody to FabFluor red reagent (Sartorius cat# 4722) for 15 minutes at 37 ℃ ...
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bioRxiv - Cancer Biology 2022Quote: ... using and the pHrodo-stained B cells (IncuCyte® pHrodo® Red Cell Labelling Kit for Phagocytosis, Sartorius). Following phagocytosis assay ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: Cells were transduced with Incucyte® Cell Cycle Green/Red Lentivirus Reagent (EF1α-Puro) (Sartorius, Cat. No. 4779) as recommended by the manufacturer and transduced cells were selected by puromycin treatment ...
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bioRxiv - Immunology 2020Quote: ... apoptosis of the PC3 GFP cell line was assessed using the annexin V red (cat n°4641, Sartorius). Culture was monitored every hour during 16 hours by Incucyte and overlapping of GFP (green ...
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bioRxiv - Immunology 2020Quote: Antigenicity assays were performed and analyzed using BLI on an Octet Red 96 System (Pall Forté Bio/Sartorius) at ambient temperature with shaking at 1000 rpm ...
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bioRxiv - Immunology 2020Quote: Epitope competition was performed and analyzed using BLI on an Octet Red 96 System (Pall Forté Bio/Sartorius) at 30°C with shaking at 1000 rpm ...
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bioRxiv - Molecular Biology 2023Quote: Antibody binding to Henipavirus F proteins was assessed using BLI on an Octet RED 384 (Sartorius, formerly ForteBio) with Forte Bio Kinetics Buffer ...
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bioRxiv - Immunology 2023Quote: Affinity measurements for the BTN2A1-BTN3A1 interaction were determined by BLI on an Octet Red 96e instrument (Sartorius). Assays were performed in black 96 well flat-bottom plates at 25°C with agitation (1000 rpm) ...
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bioRxiv - Cancer Biology 2021Quote: ... clones were also labelled transiently with NucLight Red or Green BacMam 3.0 Reagent (#4621 and 4622 respectively; Sartorius (discontinued) before seeding for the assays.
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bioRxiv - Microbiology 2021Quote: ... and CR3022 IgG to monomeric RBD and RBD-I53-50 nanoparticles was analyzed for real-time stability studies using an Octet Red 96 System (Pall FortéBio/Sartorius) at ambient temperature with shaking at 1000 rpm ...
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bioRxiv - Immunology 2020Quote: ... and RBD-I53-50 nanoparticles was analyzed for accessibility experiments and real-time stability studies using an Octet Red 96 System (Pall FortéBio/Sartorius) at ambient temperature with shaking at 1000 rpm ...
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bioRxiv - Immunology 2021Quote: Affinity measurements for VHH-huFc antibodies were performed using biolayer interferometry (BLI) using an Octet 384 Red system (Sartorius). Measurements were conducted in 10 mM phosphate (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: The binding affinities for the RBD of B-RAF and RA domain of RASSF5 of the K-RAS-GMPPNP (wild-type and A59T/E) NMR samples were measured using an Octet RED-384 biolayer interferometry biosensor instrument (BLI, FortéBio, Sartorius) running Octet Data Acquisition 9.0.0.37 ...
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bioRxiv - Molecular Biology 2024Quote: ... PBMCs were labelled with 1:1000 Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Incucyte (Sartorius) while cell death was measured by the IncuCyte Caspase 3/7 Green Apoptosis Assay Reagent as explained before ...
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bioRxiv - Immunology 2024Quote: Fab binding kinetics were measured using biolayer interferometry on an Octet 96 Red instrument (ForteBio) using FAB2G biosensors (Sartorius). C7 and C74 Fabs were diluted to 10 μg/mL in Kinetics Buffer (KB ...
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Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryoEMbioRxiv - Immunology 2024Quote: IgG-binding kinetics were measured using biolayer interferometry on an Octet 96 Red instrument (ForteBio) using AHCII biosensors (Sartorius). IgGs were diluted to 5 μg ml−1 in kinetics buffer (20mM HEPES pH7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... and ABTR#2 cells were infected at an MOI of 3-5 with the IncuCyte Nuclight Red (#4476 Sartorius) or IncuCyte Cell Cycle Green/Red Lentivirus Reagent (#4779 ...
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bioRxiv - Zoology 2022Quote: ... The complete fibroblast medium (50 mL) was composed of Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (ThermoFisher and Sartorius), MEM Non-essential Amino Acid Solution (NEAA ...
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bioRxiv - Molecular Biology 2024Quote: HEK293T cells (ATCC, cat# CRL-3216) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Sartorius cat# 01-055-1A) supplemented with 2mM glutamine (Gibco BRL) ...
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bioRxiv - Molecular Biology 2021Quote: The evaluation of binding properties between EMX-EcoCascade (analyte) and target DNAs (ligands) was performed by bio-layer interferometry (BLI) using the Octet RED 96 system (ForteBio, Sartorius BioAnalytical Instruments ...
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bioRxiv - Cell Biology 2021Quote: ... to measure cell growth cells were stained with Incucyte® NucLight Rapid Red Reagent for nuclear labeling following the manufacturer’s instructions (Sartorius). Those experiments were monitored using Incucyte live cell analysis system ...
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bioRxiv - Microbiology 2022Quote: ... Virus replication was assessed at 24 hpi by quantifying the average red fluorescence in each well using a live cell microscope (Inncucyte S3, Sartorius).
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bioRxiv - Immunology 2022Quote: Splenic CD4+ and CD8+ T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were cultured in RPMI 1640 medium (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were treated with increasing concentrations (12.5-100 μM) of TLR agonists in complete medium in the presence of IncuCyte® Cytotox Red (Sartorius), following the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... Its binding to LIF was confirmed using recombinant LIF (ACRO Biosystems, LIF-H52H3) on an Octet RED 96e biolayer interferometry instrument (Sartorius).
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bioRxiv - Cancer Biology 2024Quote: ... the media was changed to 90 µL of growth media containing 1:1000 Incucyte Nuclight Rapid Red Dye (Sartorius, 4717) and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium ...
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bioRxiv - Synthetic Biology 2023Quote: ... Four images per well were acquired at 10x magnification in the green channel (200 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hrs using an Incucyte (Sartorius). A cytotoxicity index was calculated by dividing total green fluorescence intensity per mm2 at every time point by the intensity per mm2 at the first time point ...
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bioRxiv - Synthetic Biology 2023Quote: ... One image per well was acquired at 4x magnification in the green channel (100 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hr using an Incucyte (Sartorius).
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bioRxiv - Bioengineering 2022Quote: The cytotoxicity of T cells transduced with a bi-specific CD19xCD22 CAR was determined by IncuCyte Cytotoxicity Assay with Annexin V Red Reagent for apoptosis (Sartorius) with NALM-6 cells that stably express GFP serving as target cells ...
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bioRxiv - Immunology 2024Quote: HLA-A2+ tumor cell lines were lentivirally transduced to express either Nuclight-Red or Nuclight-Green nuclear-localizing fluorescent protein (Sartorius) and sorted for their expression ...
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bioRxiv - Developmental Biology 2020Quote: ... and LSECs were supplemented with 10µg/ml FSA-FITC (prepared as described in (47)) and 1:1000 IncuCyte® NucLight Rapid Red Reagent (cat. 4717, Sartorius). Cells were imaged hourly over a period of 24 hours using the IncuCyte Cell analysis system (Essen Bioscience) ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... 100µL of the combined LIVE/DEAD® assay reagent was then added to each well and incubate for 45 min before quantification of percentage of red and green cells using the IncuCyte® ZOOM (Sartorius) system and its proprietary ZOOM 2016A software.
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Cell Biology 2024Quote: ... One hundred microliters of the combined LIVE/DEAD® assay reagent was added to each well and incubated for 45 min before quantification of percentage of red and green cells using the IncuCyte® ZOOM (Sartorius) system and its proprietary ZOOM 2016A software.
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bioRxiv - Molecular Biology 2023Quote: ... For the Incucyte assay, THP-1 or Jeko-1 cells were transduced with IncuCyte NucLight Red Lentivirus Reagent (EF-1 Alpha, Puro) (Sartorius, USA). All cell lines were repeatedly tested for the presence of mycoplasma contamination with a MycoReport Mycoplasma Detection Kit (Evrogen ...
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bioRxiv - Bioengineering 2023Quote: ... COS7 cells at a concentration of 60,000 cells/ml in Dulbecco’s Modified Eagle Medium (DMEM) with 1g/l D-glucose (Sartorius, 01-050-1A), supplemented with fetal bovine serum (Biological Industries ...
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bioRxiv - Cell Biology 2024Quote: ... MCF7 cells were cultured in high glucose (4,5 g/L) containing Dulbecco’s modified Eagle’s medium (DMEM, Sartorius, Cat # 04-007-1A) supplemented with 10% fetal bovine serum (FBS ...