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Citations for Sartorius :
51 - 100 of 246 citations for Inactive tyrosine protein kinase 7 PTK7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...
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bioRxiv - Neuroscience 2021Quote: ... At 7 DIV neurons were infected with the IncuCyte NeuroBurst Orange lentivirus under a synapsin promoter (Essen Bioscience Sartorius Cat# 4736). After 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated at 20,000 cells/ml in Geltrex-coated 96-well plates and treated with different doses of inhibitor in the presence of 1:1000 IncuCyte® Caspase-3/7 Dye for Apoptosis (Sartorius). Doubling times were calculated by determining cell counts over multiple days with the IncuCyte Live Cell Analysis System cell-by-cell module (Sartorius).
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bioRxiv - Cell Biology 2024Quote: ... Annealed iMyo01 (final concentration of 0.7 mM) was dissolved in 20 mM sodium phosphate (pH 6.5) using a centrifuge concentrator (Vivaspin 3000 MWCO; Sartorius, Göttingen, Germany). For the K+ condition ...
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bioRxiv - Cancer Biology 2023Quote: 2,000 cells per well were plated in 96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network external controller hard drive that gathered and processed image data ...
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bioRxiv - Cancer Biology 2023Quote: Analysis of apoptosis using fluorescence live cell microscopy Cells were stained with Incucyte® Caspase-3/7 Dye for Apoptosis (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Batch cultivations were performed in biological replicates (between 2-7 per condition) in 1.0 L Biostat Qplus bioreactors (Sartorius Stedim Biotech, Germany). The temperature was controlled at 28°C and pH was maintained at 6.0 (when desired ...
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bioRxiv - Immunology 2024Quote: ... Fluorescent images of live cells were obtained hourly for 7 days using an IncuCyte SX3 live cell imaging and analysis system (Sartorius Göttingen, Germany), as previously described 15.
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using Vivaspin centrifugal filters (Sartorius) of appropriate cut-offs ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100kDa MWCO Vivaspin protein concentrator (Sartorius), aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Vivaspin concentrators (Sartorius Stedin) and the sample purity and homogeneity was assessed by SDS-PAGE.
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bioRxiv - Cell Biology 2024Quote: ... the protein was concentrated using Vivaspin column (Sartorius). Pellets from KCMF1-Strep expression were resuspended in 50mM Hepes/KOH pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: The kinetics of the protein-protein interactions were determined using the bio-layer interferometry from the BLI system (Sartorius Octet BLI Discovery). Protein-protein interactions were observed by immobilizing 0.05 mg/ml of His-tagged VARP FL on a Ni-NTA biosensor or 0.05 mg/ml of biotinylated N-VARP on a streptavidin biosensor ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated using centrifugal concentrators (VivaSpin, Sartorius) and then assessed for successful labeling by measuring fluorescence using the following settings and the monochromator in the BioTek Synergy Neo2 plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT and proteins concentrated via centrifugation (Vivaspin, Sartorius). Recombinant kinase was added to 10µl of 2X kinase buffer [40mM Hepes (pH 7.5) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protein was concentrated (Vivaspin 20, 50 kDa MWCO, Sartorius), and flash frozen in liquid nitrogen until further use.
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated in a Vivaspin 20 (Sartorius) concentrator (10-kDa MWCO ...
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bioRxiv - Cancer Biology 2024Quote: ... in the presence or absence of CM collected from pre-senescent or senescent HDF and the Incucyte® green Caspase-3/7 activity reagent (Sartorius, Ann Arbor, MI, USA). Plates were analyzed at a two-hour interval for a total of 72 hours.
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bioRxiv - Biochemistry 2021Quote: ... All protein samples were concentrated using Vivaspin centrifugal concentrators (Sartorius). Denaturing gel electrophoresis was performed using NuPAGE 4-12 % Bis-Tris gels (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... the refolded protein was concentrated using a VIVAFLOW50 system (Sartorius). The concentrated sample was filtrated with 0.22 µm membrane filter ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was then concentrated (Vivaspin 30,000 MWCO, Sartorius) and ultracentrifuged in a TLA-120.2 rotor (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... Then proteins were transferred into ultrafiltration tubes (10kDa, Sartorius AG) and washed three times with 50 mM NH4HCO3 at 14000g for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using centrifugal filter devices (Vivaspin, Sartorius). Protein concentration was estimated from its absorbance at 280 nm ...
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bioRxiv - Microbiology 2024Quote: ... Protein sample was concentrated with a 10K MWCO concentrator (Sartorius) and further purified by size exclusion chromatography (Superdex 200 Increase 10/300 GL GE Life Sciences).
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bioRxiv - Immunology 2024Quote: ... Experiments were performed using Octet R2 Protein Analysis System (Sartorius) and affinity was calculated using Octet Analysis Studio Software ...
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bioRxiv - Biochemistry 2024Quote: ... using a 30kDa cut-off centrifugal protein concentrator (Vivaspin, Sartorius). The final protein concentration was estimated using a microplate spectrophotometer (Multiskan SkyHigh ...
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bioRxiv - Cell Biology 2023Quote: ... Chemiluminescent signals were detected by a WSE-6300 LuminoGraph III (ATTO, Tokyo, Japan) using EZ-ECL chemiluminescence detection kit for HRP (Sartorius, Göttingen, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were concentrated using 30 kDa cut-off Vivaspin columns (Sartorius) and their concentration was determined by their absorbance at 280 nm.
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bioRxiv - Biochemistry 2021Quote: ... Protein was concentrated to 32 mg/ml using centrifugal concentrators (Sartorius) with a relative molecular mass cut-off of 100K.
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bioRxiv - Synthetic Biology 2021Quote: ... Protein was concentrated using a Vivaspin 500 column (Sartorius, Goettingen, Germany) column following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... All proteins were diluted in Octet kinetics buffer (Sartorius, Göttingen, Germany). Antibodies were captured on anti-human Fc AHC2 tips (Sartorius) ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrated 10x using Vivaspin™ protein concentrator spin columns (Sartorius, VS15T02) and stored at 4°C.
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bioRxiv - Microbiology 2023Quote: ... The purified proteins were concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 5 or 50 kDa) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by ultrafiltration (3 kDa Vivaspin; Sartorius, Göttingen, Germany). ACE and ANSA hydrolysis activities were quantified in discontinuous HPLC-based assays at 30°C in lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: The MultiCyt® QBeads® PlexScreen Secreted Protein Assay Kit (Sartorius) was used to quantify the level of tumor necrosis factor alpha (TNF-a ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialyzed protein was concentrated with Vivaspin Turbo 4 concentrator (Sartorius) with a 100 kDa MWCO.
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bioRxiv - Neuroscience 2024Quote: Antibodies were incubated at a ratio of 1:3 antibody to FabFluor red reagent (Sartorius cat# 4722) for 15 minutes at 37 ℃ ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was concentrated using a Vivaspin centrifugal concentrator (MWCO 10 kDa, Sartorius) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated with Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva) ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were centrifuged on a 30 kDa cutoff filter device (Sartorius) and washed each thrice with UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialysed protein was spin-concentrated in a Vivaspin 6 MWCO 50,000 (Sartorius) to the desired concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein was captured at 50 nM on Ni-NTA probes (Sartorius) for 90 s after a 60 s activation step with 5 mM NiCl2 prepared in mQ water ...
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bioRxiv - Microbiology 2024Quote: Binding KDs were determined using an Octet R8 protein analysis system (Sartorius). RHA10.04 or RHA10 UCA IgG was diluted in PBST with 1% BSA to a concentration of 5µg/ml and captured on anti-human IgG-Fc biosensors (Sartorius ...