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Citations for Sartorius :
51 - 100 of 141 citations for Glypican 3 GPC3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 4°C using a 3 kDa MWCO spin concentrator (Sartorius) until the final volume was 0.5 mL ...
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bioRxiv - Cancer Biology 2021Quote: ... we used IncuCyte Caspase-3/7 Green Reagent for apoptosis (Sartorius). Crystal violet staining assay was used to assess the relative number of cells as previously described [36] ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by ultrafiltration (3 kDa Vivaspin; Sartorius, Göttingen, Germany). ACE and ANSA hydrolysis activities were quantified in discontinuous HPLC-based assays at 30°C in lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... and quantified on the iQue 3 VBR using Forecyt software (Sartorius). Phagocytic scores were calculated by multiplying the percentage of bead positive cells by the bead fluorescence GMFI of bead positive cells and dividing by 100,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... immediately followed by the addition of caspase 3/7 dye (Sartorius) at 0.5uM ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads detecting cytokines from human were analyzed on an IQue Screener Plus (Sartorius). All assays were analyzed using quintuplicate determinations.
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bioRxiv - Evolutionary Biology 2022Quote: ... IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect ...
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bioRxiv - Immunology 2024Quote: Purified IgG1 mAbs were quantified using anti–human Fab-CH1 (FAB2G) biosensors (Sartorius) as previously described (58) ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then loaded into a Vivaspin 3 KDa MWCO (Sartorius) and washed thrice with 200 µL of MS-grade H2O ...
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bioRxiv - Cell Biology 2022Quote: ... ECs and SMMCs were cultured in BioAMF-3 (01-196-1A, Sartorius).
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bioRxiv - Neuroscience 2023Quote: ... the IncuCyte Caspase-3/7 Red apoptosis reagent (1.5 µM; #4704, Sartorius) was supplemented to the cultures ...
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bioRxiv - Molecular Biology 2022Quote: Human primary keratinocytes were plated in Essen ImageLock 96-well plates (Sartorius, Göttingen, Germany) at 15,000 cells per well for migration assay and in 12-well plates at 20,000 cells per well for cell growth assay ...
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bioRxiv - Cancer Biology 2023Quote: The human plasma samples were filtered (Minisart NML syringe filters, pore size: 0.8 µm; Sartorius) and diluted with 1x Phosphate-Buffered Saline without calcium and magnesium buffer (Gibco ...
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bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
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bioRxiv - Microbiology 2021Quote: ... 3 L of water were pre-filtered through the 5 μm filter (Sartorius). The flow-through was further filtered using the 0.2 μm filter (Sterivex) ...
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bioRxiv - Developmental Biology 2020Quote: ... and sterile gelatin filters (3 μm pores and 80 mm diameter, Sartorius, Germany)[7,14] ...
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bioRxiv - Cancer Biology 2024Quote: ... Apoptosis was measured by using the green fluorescent caspase 3/7 dye (Sartorius) and determining the number of green fluorescent objects per well over time ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
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bioRxiv - Microbiology 2023Quote: We performed human plasma and avidity variant Abs’ binding using an Octet Red384 biolayer interferometer (Sartorius). We completed all the steps in 0.05% BSA and 0.002% Tween20 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM of IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, cat #4440) as well as 1:500 of Nuclight Rapid Red Dye (Sartorius ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 μM Caspase 3/7 dye was included in the media (Sartorius #4704). The number of red nuclei (indicating active caspase 3/7 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by ultrafiltration at 3-bar with a 100 kDa filter (Sartorius, Goettingen, Germany). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1µg/µl of CpG and 0.9ul of caspase-3/7 dye (Sartorius, 4440) were added to the well for 72hrs incubation in the SpectraMax M5 and Lmax microplate reader ...
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bioRxiv - Biochemistry 2023Quote: ... The 3’-biotinylated RNA was immobilized on an Octet® streptavidin (SA) Biosensor (Sartorius). 3 μg of biotinylated RNA were incubated per sensor for 300 s ...
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bioRxiv - Microbiology 2019Quote: ... Biomass was harvested via centrifugation (3090 g, 10min) or by filtration (Sartorius, format 3 hw), freeze-dried and used directly for the analysis of β-1,3-glucan content according to (38) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Triads (n = 3) were weighed to the nearest 0.1 mg (Sartorius Genius ME 235P-OCE) before transfer to a respirometry chamber (a glass cylinder ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting supernatant was concentrated using a Vivaspin device (MWCO, 3 kDa; Sartorius Stedim Biotech) and kept for Western immunoblotting analyses ...
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bioRxiv - Biochemistry 2022Quote: ... Flow cytometric data were acquired on an iQue 3 high-throughput flow cytometry system (Sartorius). Data were analyzed using FlowJo v10 (FlowJo).
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bioRxiv - Immunology 2024Quote: ... beads were resuspended in FACS buffer and acquired on flow cytometer iQue®3 (Sartorius). Population gating and analysis of fluorescence intensity were performed with iQue Forecyt software ...
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bioRxiv - Immunology 2024Quote: ... beads were resuspended in FACS buffer and acquired on flow cytometer iQue®3 (Sartorius). Population gating and analysis of fluorescence intensity were performed with iQue Forecyt software ...
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bioRxiv - Biochemistry 2024Quote: ... and concentrated over a 3 kDa MWCO membrane using a Vivaspin 15 centrifugal device (Sartorius). The final Ws cytochrome c552 fraction was buffer exchanged to 5 mM Na-phosphate pH 7.0 using a desalting PD-10 column (Cytiva) ...
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bioRxiv - Bioengineering 2023Quote: ... the formulated bulk sample was collected in 3 L Flexboy 2D bag (Sartorius Stedim, France) and filled into pre-sterile syringe (Schott ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
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bioRxiv - Cell Biology 2024Quote: Human PSCs including iPSCs and ESCs were cultured in NutriStem® hPSC XF Medium (Sartorius, 05-100-1A) after coating with 50 μg/μL Vitronectin (VTN-N ...
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bioRxiv - Plant Biology 2021Quote: ... and loaded onto an ultrafiltration column (Vivaspin 3 or 10 kDa MWCO, PES, Sartorius, Palaiseau, France) for concentration and buffer exchange at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Mixed media were subsequently concentrated through spin columns with a cutoff of 3 kDa (Vivaspin, Sartorius). Mixed proteins were separated on a 4-20% Mini-PROTEAN TGX precast gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to 3 mg/ml using a 50 kDa cutoff concentrator (Vivaspin, Sartorius). The purified protein was flash frozen in liquid nitrogen and stored at − 80 °C.
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bioRxiv - Developmental Biology 2022Quote: ... EpiLC differentiation medium was added and supplemented with 5µM of Caspase 3/7 Green dye (Sartorius) to detect for apoptosis in live cells ...
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bioRxiv - Microbiology 2023Quote: ... The filtered medium was then concentrated by ultrafiltration using Vivaspin 20 (3 kDa cut-off, Sartorius). Protein concentration was determined using a BCA protein assay kit (Beyotime ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants containing the fibrils were recovered and concentrated on vivaspin® 3 KDa (Sartorius, VS04T91) at 3,000 g ...
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bioRxiv - Immunology 2023Quote: ... they were concentrated using Vivaspin centrifugal concentrators with a cut-off of 3 kDa (Sartorius, VS2091) and stored at -20°C ...
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bioRxiv - Immunology 2023Quote: ... Human antibodies (5 μg/mL or 7.5 µg/mL) were captured on pre-conditioned anti-human IgG Fc-capture biosensors (AHC, Sartorius) for 600 s ...
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 2-3 mg/ml using Vivaspin 15R concentrators (2 kDa MWCO HY, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... but visually identifiable amount (Supplementary Figure 3) by weighing powder on a microbalance (Sartorius BP 210 D). One hour before the release ...
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bioRxiv - Microbiology 2022Quote: ... Homogenates were centrifuged for 3 min at 17,000g and filtered using a 0.8 μm PES filter (Sartorius). Filtrates were treated with micrococcal nuclease (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diafiltered at 4°C using 3 kDa cutoff VivaSpin®500 centrifuge filters (Sartorius) for 3 cycles of 20 min at 14,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Proteolytic DEVD cleaving activity was determined using the Incucyte Caspase-3/7 Red Dye for Apoptosis (Sartorius) as described in the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% nonfat dry milk) to 100 nM and immobilized for 60 s to anti-human IgG Fc capture biosensors (Sartorius). Sensors were transferred to assay buffer for 30 s to set the baseline ...
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bioRxiv - Microbiology 2021Quote: ... cell culture supernatant was centrifuged for 3 min at 17,000 × g and filtered with a 0.8-μm PES filter (Sartorius). The filtrate was treated with benzonase (Novagen ...