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Citations for Sartorius :
51 - 100 of 113 citations for BENZOFURAN 6 7 DIOL since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Subsamples were weighed (20 mg ± 6 mg) on a fine scale balance (Entris, Sartorius). Together with 6 negative controls (200 μl lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... the samples were concentrated using filter columns (Vivaspin 6, 100,000 MWCO PES, Sartorius, Germany) at 4 °C with a maximal speed of 4,000 x g and washed twice with DBCM2 salt solution ...
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bioRxiv - Pathology 2024Quote: ... Extraneous dye (270,000 MW) was removed using Vivaspin 6 centrifugal concentrators (Sartorius, 300,000 MWCO). Nanoparticles were further purified by a SuperMag Separator (Ocean NanoTech ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell proliferation was recorded for up to 7 days every 4 hours by the IncuCyte S3 Live-Cell Analysis System (Sartorius) with a phase-contrast microscope at 10X magnification ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ and CD8+ T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were cultured in RPMI 1640 medium (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... was monitored by imaging every 2 hours for 7 days at 10x magnification with the ‘phase’ channel using the Incucyte live-cell analysis system S3 (Sartorius). Automatic image segmentation and analysis of the phase contrast images was performed by the Incucyte base analysis software (version 2020B).
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bioRxiv - Neuroscience 2021Quote: ... At 7 DIV neurons were infected with the IncuCyte NeuroBurst Orange lentivirus under a synapsin promoter (Essen Bioscience Sartorius Cat# 4736). After 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated at 20,000 cells/ml in Geltrex-coated 96-well plates and treated with different doses of inhibitor in the presence of 1:1000 IncuCyte® Caspase-3/7 Dye for Apoptosis (Sartorius). Doubling times were calculated by determining cell counts over multiple days with the IncuCyte Live Cell Analysis System cell-by-cell module (Sartorius).
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bioRxiv - Cell Biology 2024Quote: ... Annealed iMyo01 (final concentration of 0.7 mM) was dissolved in 20 mM sodium phosphate (pH 6.5) using a centrifuge concentrator (Vivaspin 3000 MWCO; Sartorius, Göttingen, Germany). For the K+ condition ...
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bioRxiv - Cancer Biology 2023Quote: 2,000 cells per well were plated in 96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network external controller hard drive that gathered and processed image data ...
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bioRxiv - Cancer Biology 2023Quote: Analysis of apoptosis using fluorescence live cell microscopy Cells were stained with Incucyte® Caspase-3/7 Dye for Apoptosis (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was then concentrated using Vivaspin® 6 100,000 MWCO concentrator columns (Sartorius). The sample was then purified via size exclusion chromatography using a Superose™ 6 Increase column (GE Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Immunology 2022Quote: ... and the eluate was collected into a Vivaspin 6 50 kDa MWCO column (Sartorius, V10631), which was centrifugated at 2,113 g for five minutes to concentrate the antibodies ...
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bioRxiv - Microbiology 2020Quote: Batch cultivations were performed in biological replicates (between 2-7 per condition) in 1.0 L Biostat Qplus bioreactors (Sartorius Stedim Biotech, Germany). The temperature was controlled at 28°C and pH was maintained at 6.0 (when desired ...
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bioRxiv - Immunology 2024Quote: ... Fluorescent images of live cells were obtained hourly for 7 days using an IncuCyte SX3 live cell imaging and analysis system (Sartorius Göttingen, Germany), as previously described 15.
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bioRxiv - Molecular Biology 2021Quote: ... the volume was adjusted to 5 mL and transferred to a Centrifugal Concentrator (Vivaspin 6; Sartorius). The retentate was adjusted to 120 µL prior to analysis.
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bioRxiv - Biophysics 2020Quote: ... were collected and concentrated using a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius) to a final concentration ∼2.5 mg mL-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cellular confluence in all wells was monitored every 6 h using an IncuCyte ZOOM (Sartorius). In each plate ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing Ldh-EtfAB were pooled and concentrated via ultrafiltration (Vivaspin 6, 30 kDa Cut-off, Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2023Quote: ... which took place overnight on arrival at 4°C (cold room) using 6 Vivaflow200 filtration units (Sartorius) operating in two parallel series ...
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bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
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bioRxiv - Microbiology 2024Quote: ... The plates were imaged at 6 h intervals using the Incucyte SX5 Live-Cell Analysis System (Sartorius) to determine the area occupied by GFP-positive cells ...
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bioRxiv - Systems Biology 2022Quote: ... and then 6 hours after doxorubicin treatment was moved to an incubator connected to an Incucyte microscope (Sartorius). Brightfield images of wells were taken 6 ...
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bioRxiv - Cell Biology 2022Quote: ... 6 ml of the crude CM was passed through a 300 kDa Vivaspin concentrator (Sartorius AG, Göttingen, Germany) by centrifugation at 3,000 g for ∼15 minutes (or until the retentate was ∼300 µl) ...
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bioRxiv - Microbiology 2020Quote: ... Eluted proteins were concentrated using an ultrafiltration membrane with a 10 kDa molecular weight cutoff (Vivaspin 6, Sartorius). To reconstitute the BAM-DolP complex in vitro ...
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6.0) using a centrifugal concentrator with a molecular weight cutoff of 10 kDa (Vivaspin 6, VS0602, Sartorius). The presence of the fucosidase (62 kDa ...
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bioRxiv - Cancer Biology 2024Quote: ... in the presence or absence of CM collected from pre-senescent or senescent HDF and the Incucyte® green Caspase-3/7 activity reagent (Sartorius, Ann Arbor, MI, USA). Plates were analyzed at a two-hour interval for a total of 72 hours.
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... Purified V-ATPase in buffer containing LMNG was concentrated to 1.9 mg/mL with a 100 kDa MWCO VivaSpin 6 centrifugal concentrator at 2000 ×g for 15 min (Sartorius), followed by a VivaSpin 500 concentrator at 12,000 ×g for 2 min.
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bioRxiv - Biophysics 2023Quote: ... Fractions containing Ub were concentrated and buffer exchanged into pH 6.8 buffer (see above) using Vivaspin 6 centrifugal concentrators with a molecular weight cutoff (MWCO) of 5000 Da (Sartorius). Purified proteins were frozen at −80°C.
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bioRxiv - Microbiology 2023Quote: ... whole well images were taken at 6 h intervals using an Incucyte Zoom S3 live cell imaging system (Sartorius).
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bioRxiv - Microbiology 2023Quote: ... Total green cell count was measured every 6 h using an Incucyte Zoom S3 live cell imaging system (Sartorius) and normalised for confluency variation ...
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Bioengineering 2024Quote: ... and arginine (pH 6) to a final concentration of ≥200 mg/mL using the Ambr Crossflow system (Sartorius, Germany).
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was then concentrated to the approximate concentration of 10 mg/ml using a Vivaspin 6 3,000 MWCO PES (Sartorius) membrane ultrafiltration tube.
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bioRxiv - Biochemistry 2024Quote: ... HNV-F proteins with the twin-strep tag at 6 µg/mL were captured onto Streptavidin (SA) Biosensors (Sartorius) for 300s ...
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bioRxiv - Genomics 2024Quote: ... The resulting mixture was further concentrated to approximately 250 μl using a Vivaspin 6 centrifuge concentrator (Sartorius Mechatronics, UK) with a 5000 Da MWCO PES membrane ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was transferred to a Vivaspin® 6 centrifuge unit with a 100 kDa MWCO PES membrane (Sartorius).
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bioRxiv - Biochemistry 2022Quote: ... The fractions were checked via SDS-PAGE and the cleanest Chz1-containing fraction was concentrated using a 10 kDa cutoff Vivaspin 6 concentrator (Sartorius) at 3,000 g at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclear suspension was centrifugated for 30 min at 15 000 g and supernatant was concentrated using Vivaspin 6 (10 kDa MWCO) centrifugal concentrator (Sartorius). Aliquots of concentrated nuclear extracts were frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Genetics 2023Quote: ... and cells were imaged live every 6 hours using the IncuCyte® ZOO system for automated live cell analysis (Sartorius). Wound closure was analysed by measuring the spatial cell density in the wound area relative to the spatial cell density outside of the wound expressed as the Relative Wound Density (% ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through was collected and concentrated to 6-10 mg/ml using a 100 kDa molecular weight (MW) cutoff centrifugal concentrator (Sartorius) and loaded onto a Superdex 200 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Eluent from the first two elution fractions after the elimination of dead volume were concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Eluent from the first two elution fractions after the elimination of dead volume were concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... the fractions obtained by affinity chromatography were pooled and concentrated using conical membrane ultrafiltration tubes (Vivaspin 6 mL 10 kDa MWCO, Sartorius) to 7 mg/mL (2 mL) ...