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Citations for Sartorius :
51 - 100 of 361 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Binding kinetics of WS6 IgG and related S2-stem helix antibodies to diverse CoV 2P-stabilized spike proteins (S2Ps) were measured by biolayer interferometry using an Octet HTX instrument (Sartorius). All assays were performed in tilted 384-well plates (Geiger Bio-one ...
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bioRxiv - Immunology 2021Quote: ... biotinylated S1 binding mAbs at 25 µg/mL were first loaded on High Precision Streptavidin SAX biosensors (Sartorius Corporation) for 10 sec ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for binding using streptavidin (SA) biosensors (Sartorius/ForteBio) loaded to a threshold of 0.8nm of SARS-CoV- 2 ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding was assessed using a control anti-influenza haemagglutinin mAb (CH65) for reference subtraction and the binding curves were analyzed using the ForteBio Data Analysis Software 10.0 (Sartorius). Binding responses (nm ...
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bioRxiv - Biophysics 2024Quote: Binding of anti-TcPOP mAbs to TcPOP were measured by kinetic experiments carried out on an Octet R4 (Sartorius). All samples were buffer exchanged into Sartorius Kinetics Buffer ...
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bioRxiv - Immunology 2024Quote: Fab binding kinetics were measured using biolayer interferometry on an Octet 96 Red instrument (ForteBio) using FAB2G biosensors (Sartorius). C7 and C74 Fabs were diluted to 10 μg/mL in Kinetics Buffer (KB ...
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bioRxiv - Systems Biology 2021Quote: ... A 2 L bioreactor equipped with a Sartorius BIOSTAT B® fermentation controller (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Systems Biology 2022Quote: Co-culture experiments were performed in lab-scale stirred bioreactors (Biostat B-Twin, Sartorius). The processes were performed with a working volume of 1L at a constant temperature of 30°C ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing biotinylated primer bound to monovalent streptavidin were subsequently annealed to a 5’ biotinylated DNA template before binding to streptavidin biosensors (Sartorius). 200 nM of DNA substrates were added in the loading step ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Synthetic Biology 2019Quote: pH-Controlled bioreactor batch fermentations were conducted with a Biostat B+ (Sartorius Stedim, NY, USA) dual 1.5 L fermentation system at a working volume of 1 L M9 hybrid medium ...
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bioRxiv - Microbiology 2021Quote: Cultures in segregostat mode were performed in lab-scale stirred bioreactor (Biostat B-Twin, Sartorius) with 1 L working volume ...
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bioRxiv - Bioengineering 2024Quote: The three-step batch cocultivation was done by using Biostat B Plus bioreactor (Sartorius, Germany) equipped with pH-meter (Hamilton ...
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bioRxiv - Microbiology 2022Quote: The affinity between each antibody and spike protein was measured by bio-layer interferometry (BLI) with a BLItz Biolayer Interferometer System (Sartorius, Goettingen, Germany).
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for their competition of binding with one another using anti-Penta HIS (His1K) biosensors (Sartorius/ForteBio). His1K tips were pre-quenched with buffer containing 10nM biotin ...
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bioRxiv - Immunology 2020Quote: ... and S309 for binding to soluble SARS-CoV-2 S trimer was assessed using the ForteBio Octet HTX (Sartorius Bioanalytical Instruments). All reagents were diluted to 100 nM in PBSF ...
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bioRxiv - Biochemistry 2023Quote: ... The optical interference pattern of white light was used to measure binding kinetics with the Octet® BLI Label-Free Detection System (Sartorius). MDA5 and LGP2 dissociation was measured by flowing buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μM of small-molecule targets with biotin conjugated were used as ligands to be immobilized to the Octet Streptavidin biosensors (Sartorius). Titration experiments were performed in 25C ...
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bioRxiv - Cancer Biology 2023Quote: ... of PD-L1 protein/PD-L1 antibody was determined by Octet Biolayer interferometry (BLI) using the Octet RED384 system (Sartorius, Bohemia, NY, USA). Briefly ...
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bioRxiv - Bioengineering 2021Quote: Bioreactor cultivation was performed as duplicates in 2 L Biostat B-DCU bioreactors (Sartorius AG, Germany) with a working volume of 1.5 L ...
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bioRxiv - Microbiology 2022Quote: ... thermolithotrophicus was continuously grown in a 10 L fermenter (BIOSTAT® B plus, Sartorius, Göttingen, Germany) under diazotrophic conditions with either 10 mM Na2SO3 or Na2SO4 as sulfur source instead of Na2S ...
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bioRxiv - Immunology 2023Quote: ... The GRAd32 S-2P vector was expanded in a 2 L Bioreactor (Biostat B DCU; Sartorius). The titer of virus contained in the bulk cell lysates was quantified by qPCR ...
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bioRxiv - Biochemistry 2022Quote: ... protein solutions were concentrated to about 12 mg ml-1 using a centricon centrifugal filter unit (Sartorius Stedim). The protein concentration was estimated by measuring the absorbance at 280 nm.
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bioRxiv - Developmental Biology 2024Quote: ... and 100,000 cells were resuspended in growth factor-free CNCC early maintenance media and seeded in an IncuCyte ImageLock 96-well plate (Sartorius 4379) in triplicate and incubated for 48 hours at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... A D-Optimal design with quantitative and qualitative factors was created with MODDE Pro software version 12.1 (Sartorius AG, Göttingen, Germany) (Supplemental Table 1) ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: Real-time assessment of CXCL4 and CXCL17 binding to heparin dp8 and heparan sulfate was assessed via bio-layer interferometry (BLI) on an Octet Red96 system (Sartorius, Goettingen, Germany) using a methodology adapted from a previous study [48] ...
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bioRxiv - Systems Biology 2021Quote: ... faecalis V583 ΔglnA was grown in glucose-limited chemostat cultures in Biostat B Plus benchtop bioreactors (Sartorius) in 750 ml CDM-LAB with a dilution rate of 0.15/h at 37 °C and gassing with 0.05 L/min nitrogen and stirring with 250 rpm ...
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bioRxiv - Immunology 2020Quote: ... Vectors were then expanded up to a production in 2L Bioreactor (Biostat B DCU, Sartorius Stedim Biotech), whereby suspension cells were seeded at 5e5 cells/mL ...
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bioRxiv - Neuroscience 2023Quote: In vivo: Tissue was lysed using a Mikro-Dismembrator S (B. Braun Biotech International GmbH, Sartorius group) in TRIzol (Thermo Fischer Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell culture was inoculated at OD600 0.3 in the 2 L bioreactor (Biostat B, Sartorius, Germany) including the 1 L modified M9 minimal medium ...
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... using and the pHrodo-stained B cells (IncuCyte® pHrodo® Red Cell Labelling Kit for Phagocytosis, Sartorius). Following phagocytosis assay ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Bioengineering 2020Quote: Batch experiments were performed using a 2 L bioreactor equipped with a Sartorius BIOSTAT B® fermentation controller (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2020Quote: Aerobic fermentor growth of ΔssaACB cell culture was achieved using a BIOSTAT® B bioreactor (Sartorius Stedim, Göttingen, Germany) and samples were collected as described in Puccio and Kitten (2020) ...
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bioRxiv - Biochemistry 2022Quote: ... and then the supernatant was concentrated and exchanged into Buffer B (25 mM HEPES pH 7.5 and 150 mM NaCl) using a Hydrosart Ultrafilter system (Sartorius). The following purification steps for anti-BRIL Fab were the same as that of BRIL-SLC19A1 ...
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bioRxiv - Bioengineering 2022Quote: Fed-batch bioreactor experiments were performed in 2-L bench top glass fermenters (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Microbiology 2019Quote: ... Residual proteins were then eliminated by filtration on 0.22-µm high-protein-retention Ministart syringe filters (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2020Quote: ... with buffer B Purity was checked via SDS-PAGE with Coomassie Staining and concentration was performed using Vivaspin Turbo apparatus (Sartorius) with a 5 kDa molecular weight cut-off.
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bioRxiv - Microbiology 2023Quote: ... at a shaking speed of 150 rpm and used to start the batch phase in a lab-scale stirred bioreactor (Biostat B-Twin, Sartorius) total volume ...
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bioRxiv - Biochemistry 2022Quote: The bioreactor cell culture experiments were conducted in a 3L glass bioreactor using Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 1.75L ...
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bioRxiv - Plant Biology 2024Quote: ... FLVB and FLVA/B were analyzed by SDS-page and then concentrated by ultra filtration (Sartorius, Vivaspin, 10 kDa cutoff). Analysis of different purification steps and quality of protein purifications was also assessed by Immunoblot analysis with custom-made α-FLVA ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Microbiology 2019Quote: ... adapted by us to the use of 100 µg protein for digestion with sequencing-grade trypsin in 50:1 ratios in Vivacon 10k filters (Sartorius AG, Germany) (28) ...
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bioRxiv - Immunology 2019Quote: ... Worm culture supernatants collected over 1 week were further concentrated using centrifugal protein concentrators with a 5 kDa MWCO (Vivaspin, Sartorius, Göttingen, Germany) to obtain the final ...
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bioRxiv - Plant Biology 2023Quote: The biolayer interferometry was performed as previously described (Xie et al., 2022) using Octet® R2 Protein Analysis System (Sartorius, Figure 1) or Gator-plus (Gator Bio ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...