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Citations for Sartorius :
851 - 900 of 984 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cytotoxicity was quantified as reduction of GFP+ area over time via imagining in Incucyte S3 Live-Cell Analysis System (Sartorius).
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bioRxiv - Microbiology 2023Quote: The sterile filtered fecal supernatant was concentrated using centrifugal filters Centrisart with a filter cut-off at 100 kDA (Sartorius) by centrifugation centrifuged at 1,500 x g at 4ºC (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Cell Biology 2023Quote: Isolated ECs were seeded onto 96 well-plates pre-coated with fibronectin in EGM-2 and the proliferation assay was performed for 60 hours in the Incucyte proliferation assay according to (21) (Sartorius). The analysis of the cell proliferation was performed by the Incucyte Basic Analyzer software.
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bioRxiv - Microbiology 2023Quote: ... monoclonal antibodies or VHHs were added to transfected cells at a final concentration of 1 µg/ml and GFP expression was monitored hourly over time with an Incucyte Zoom live cell analysis device (Sartorius) and analyzed with the provided Incucyte software.
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bioRxiv - Cancer Biology 2023Quote: ... Its binding to LIF was confirmed using recombinant LIF (ACRO Biosystems, LIF-H52H3) on an Octet RED 96e biolayer interferometry instrument (Sartorius).
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bioRxiv - Bioengineering 2023Quote: ... to CD123 WT ECD and variants (analytes in solution) was performed using Streptavidin capture biosensor (SA) (Sartorius, PN: 18-5019). CaptureSelect™ Biotin Anti-LC-kappa (Murine ...
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bioRxiv - Microbiology 2023Quote: The sterile filtered culture/fecal supernatant was concentrated using centrifugal filters Centrisart with a filter cut-off at 100 kDA (Sartorius) by centrifugation centrifuged at 1,500 x g at 4°C (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Microbiology 2023Quote: ... Final separation was achieved following concentrating the Heparin elution to approximately 8 mg/mL using a 30 kDa MWCO Vivaspin filter (Sartorius), by applying the sample to a Superdex 200 10/300 GL (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... 7500 cells per genotype were seeded in 96-well plates in both media conditions and proliferation was monitored via the IncuCyte Zoom imaging platform (Sartorius) for up to 72 hours ...
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bioRxiv - Microbiology 2023Quote: ... Flow through of the 0.22 µm filters was subsequently concentrated using tangential flow filtration with a 100 kDa cassette (Sartorius Stedim). The concentrated samples were stored at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through was collected and concentrated to 6-10 mg/ml using a 100 kDa molecular weight (MW) cutoff centrifugal concentrator (Sartorius) and loaded onto a Superdex 200 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Bioengineering 2022Quote: The cytotoxicity of T cells transduced with a bi-specific CD19xCD22 CAR was determined by IncuCyte Cytotoxicity Assay with Annexin V Red Reagent for apoptosis (Sartorius) with NALM-6 cells that stably express GFP serving as target cells ...
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bioRxiv - Bioengineering 2023Quote: ... This experiment was carried out in a commercial stirred tank bioreactor (STBR) of 2 l total volume (Sartorius AG, Germany) and a working volume of 1 l was used ...
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bioRxiv - Systems Biology 2023Quote: ... Cell dry weight was determined by vacuum filtration of 1 mL of samples on pre-weighed 0.45 µm filter membranes (Sartorius Biolab), followed by 15 min microwaving at 325 W and placement in a desiccator for at least 3 days.
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bioRxiv - Zoology 2023Quote: ... Fresh body weight (BW) of adults was obtained within 24 hours of adult emergence using an electronic microbalance (Sartorius CP225-D: Sartorius AG ...
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bioRxiv - Molecular Biology 2023Quote: ... The monodisperse peak of the complex was collected and concentrated with a 50 kDa cut-off concentrator (Vivaspin 2, Sartorius) and subsequently used for crystallization in LCP.
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bioRxiv - Bioengineering 2023Quote: CAR T cell cytotoxicity was evaluated over a variable period of days using the S3 IncuCyte Live Cell Imager (Sartorius). To begin ...
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bioRxiv - Synthetic Biology 2024Quote: ... The RFP+ count per well was quantified every two hours over a 40-hour span using IncuCyte S3 live-cell imaging (Sartorius). A375 cell growth was calculated as the number of RFP+ objects at a given time point ...
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bioRxiv - Cell Biology 2024Quote: ... Buffer exchange from iodixanol to Ringer’s lactate buffer was then carried out using Vivaspin 20 columns (Sartorius, VS2042, Göttingen, Germany). To obtain high virus titers ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated at the indicated temperature and recovery was monitored by examination of GFP expression on a live-cell microscope (Incucyte SX5; Sartorius). The optimized protocol is presented in Supplementary Data 2.
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... before percent of GFP-positive cells was determined by high-throughput flow cytometry (Intellicyt iQue Screener PLUS, Sartorius, Gottingen, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... the number of fluorescent AML cells was assessed during 48 h using the IncuCyte S3 Live Cell imaging system (Sartorius) in the non-adherent cell-by-cell mode 20X objective ...
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bioRxiv - Immunology 2024Quote: ... The mixture was gently resuspended for 60 sec and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
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bioRxiv - Immunology 2024Quote: ... propidium iodide was used to assess tumor cell death using Incucyte S3 Live-Cell Analysis System v2021A (Sartorius, Göttingen, Germany). Cell death was measured by calculating propidium iodide positive (red ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was changed to 90 µL of growth media containing 1:1000 Incucyte Nuclight Rapid Red Dye (Sartorius, 4717) and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium ...
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bioRxiv - Microbiology 2024Quote: The sterile filtered fecal supernatant was concentrated using Centrisart centrifugal filters with a filter cut-off at 100 kDA (Sartorius) by centrifugation at 1,500 x g at 4 °C (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Immunology 2024Quote: ... 10% FBS was added to the cells and real-time cell death analysis was performed using IncuCyte S3 Live-Cell Analysis instrument (Sartorius).
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bioRxiv - Cancer Biology 2023Quote: The cell viability percentage for each concentration was assessed with an XTT-based cell proliferation kit (Sartorius, Beit Haemek, Israel), then entered into SynergyFinder web application (v 2.0) ...
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bioRxiv - Microbiology 2020Quote: ... and then a volume of 1.8 – 4.5 l of filtrate from the pre-filtration was filtered under mild vacuum (<100 mm Hg) through a 0.2 μm isopore polycarbonate filter (Sartorius, Göttingen, Germany) to collect microbial biomass ...
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of confluency was assessed and automatically calculated based on the images acquired with IncuCyte® Zoom Live-Cell Analysis System (Sartorius) both at the day of compound addition (day 0 ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Biochemistry 2021Quote: A stock solution of sfGFP-ACE2 was diluted to at 10 μg/ml for immobilization onto High Precision Streptavidin (SAX) biosensors (Sartorius, Germany) in assay buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Plant Biology 2021Quote: ... the pH of the hydroponic culture media of the 5 treatments was measured using a pH meter (PB-10, Sartorius, Germany).
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bioRxiv - Microbiology 2020Quote: ... then 100 mL of the appropriately diluted sample was filtered through a cellulose nitrate membrane filter with a pore size of 0.45 μm (Sartorius, Goettingen, Germany) using a vacuum pump ...
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bioRxiv - Plant Biology 2020Quote: ... Material for analyses was obtained from seeds pre-cooled for 15 min in 5-mL Teflon capsules (Sartorius GmbH, Göttingen, Germany), containing a 10 mm-diameter agate bead ...
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bioRxiv - Systems Biology 2021Quote: ... DW was finally obtained by measuring the mass weight difference of the tube with a BP121S analytical balance (Sartorius, Goettingen, Germany). OD600 was measured at each sampling time using a diluted volume of sample and a Biophotometer spectrophotometer (Eppendorf ...
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bioRxiv - Biochemistry 2022Quote: ... A template with a biotin-TEG at the 5’-end was annealed to the primers (Table 2) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). Primers were added at two-fold molar excess over the template ...
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bioRxiv - Biochemistry 2022Quote: ... A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1) was prepared by ligation and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG) at 50 nM concentration for 7 min at 500 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... The clear supernatant was microfiltered (0.22 µm, PES) and concentrated to 50 mL using a Sartorius Vivacell 250 PES Centrifugal Concentrator (Sartorius, Göttingen, Germany) with a MWCO of 100 kDa and a pressure of 4 bar ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2021Quote: ... Full-length D-serFS was then concentrated to 200-300 μM using centricons (Vivaspin 500, 10 kDa cutoff, Sartorius Stedim Biotech). For anchoring of optical sensors in brain tissue ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Immunology 2021Quote: ... Process development was performed using the high throughput multi-parallel Bioreactor Ambr 15 and Ambr 250 (Sartorius Stedim Biotech, Göttingen, Germany). Protein production at pilot scale for nonclinical trial material was archived in 500L Bioreactor (BIOSTAT STR®500 ...
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bioRxiv - Cancer Biology 2022Quote: ... The total area occupied by spheroids (total area um2/image) was calculated using the Incucyte® Spheroid Analysis Software module (Sartorius).
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bioRxiv - Microbiology 2022Quote: ... The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm, Sartorius Stedim Biotech GmbH). The biomass was washed on the filter with 10 mL of 60% methanol and stored at -70 °C until metabolite extraction.
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bioRxiv - Cell Biology 2022Quote: ... CM ultrafiltration and concentration was carried out by tangential flow filtration (TFF) using a Vivaflow 50 R 10,000 MWCO Hydrosart membrane (Sartorius, Göttingen, Germany) and a Masterflex L/S Easy Load machine (Antylia Scientific ...