Labshake search
Citations for Sartorius :
551 - 600 of 612 citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: HeLa and MDA-MB-231 WT and ARPC5-KO cells were seeded in a 96-well ImageLock plate (Sartorius #4806) and incubated for 24 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... The RFP+ count per well was quantified every two hours over a 40-hour span using IncuCyte S3 live-cell imaging (Sartorius). A375 cell growth was calculated as the number of RFP+ objects at a given time point ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated at the indicated temperature and recovery was monitored by examination of GFP expression on a live-cell microscope (Incucyte SX5; Sartorius). The optimized protocol is presented in Supplementary Data 2.
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... before percent of GFP-positive cells was determined by high-throughput flow cytometry (Intellicyt iQue Screener PLUS, Sartorius, Gottingen, Germany).
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bioRxiv - Microbiology 2023Quote: ... Plates of infected macrophages were imaged in triplicate per well in an Incucyte S3 Live-Cell Analysis Platform (Sartorius #4647). Phase and fluorescence images were collected every hour per well using a 20X objective ...
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bioRxiv - Cancer Biology 2023Quote: ... was monitored by imaging every 2 hours for 7 days at 10x magnification with the ‘phase’ channel using the Incucyte live-cell analysis system S3 (Sartorius). Automatic image segmentation and analysis of the phase contrast images was performed by the Incucyte base analysis software (version 2020B).
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bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
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bioRxiv - Physiology 2024Quote: ... Cultures were monitored for colony formation from 2 to 8 weeks after plating using Incucyte live cell imaging system (Sartorius). Wells with single colonies were harvested and cell cultures were expanded for making stocks and for DNA isolation ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5 x 103 cells/well were plated in FBS-free media in the upper chamber of a 96-well Incucyte ClearView cell migration plate (8 µm pore size; #4582 Sartorius). Cells were incubated at 37°C for 45 minutes to allow cells to settle on the membrane before adding RPMI-1640 media supplemented with 10% FBS to the bottom chamber ...
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation assays of the combination of GEM and clinical ATR inhibitors were performed using the Incucyte live-cell imaging platform (Sartorius). AsPC-1 cells were seeded at 5000 cells/well into 96-well Eppendorf flat bottom plates and allowed to attach overnight ...
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bioRxiv - Immunology 2024Quote: ... The collagen suspension was mixed carefully with the cells in a ratio of 4:1 and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
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bioRxiv - Immunology 2023Quote: ... measuring the total fluorophore area per well every 2h h using an Incucyte® S3 Live-Cell Analysis System (Sartorius). The data from each well were normalized to the first measurement (0h).
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bioRxiv - Cancer Biology 2024Quote: ... Images were taken every 15 min for 48 hrs on an Incucyte S3 Live-cell analysis system (Sartorius, 4X/0.2). The area covered by migrating cells was measured with the Incucyte analysis program (Sartorius).
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of confluency was assessed and automatically calculated based on the images acquired with IncuCyte® Zoom Live-Cell Analysis System (Sartorius) both at the day of compound addition (day 0 ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... 100µL of the combined LIVE/DEAD® assay reagent was then added to each well and incubate for 45 min before quantification of percentage of red and green cells using the IncuCyte® ZOOM (Sartorius) system and its proprietary ZOOM 2016A software.
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bioRxiv - Cancer Biology 2022Quote: ... tumor cells transfected with plasmids containing eGFP were treated and co-cultured as described above in the IncuCyte® SC5 Live-Cell Analysis system (Sartorius). Plates were scanned with a 10x objective using phase contrast channel as well as the green fluorescent channel for 42 h every 6 h ...
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bioRxiv - Cell Biology 2020Quote: ... Live cell brightfield and fluorescent images for time-lapse experiments were acquired using the Incucyte® S3 Live Cell Analysis System (Essen Bioscience / Sartorius) running version 2019A (20191.1.6976.19779) ...
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bioRxiv - Neuroscience 2020Quote: ... Four fluorescent images per well with 300ms red channel acquisition time were taken every 15 minutes in total for three-hours using a 20x objective with the IncuCyte® S3 Live-Cell Analysis System (Sartorius). At the end of the assay ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
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bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
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bioRxiv - Microbiology 2022Quote: ... Fusion events were visualized for 24 h at 20 min intervals using the IncuCyte® S3 Live-Cell Analysis System (Sartorius). Phase contrast and GFP images (4 per well ...
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bioRxiv - Microbiology 2023Quote: High cell density fed-batch fermentation for production of Nanobody molecules was performed at 0.25 L (Ambr250 MO units, Sartorius Stedium Biotech), 2 L or 5 L scale (Biostat B-plus and B-DCU units ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants were harvested and clarified by centrifugation (3000xg for 20 minutes) or using Sartoclear Dynamics® Lab V (Sartorius). Supernatants were then filtered at 0.2 μm using Nalgene Rapid Flow sterile single using vacuum filter units (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... COS7 cells at a concentration of 60,000 cells/ml in Dulbecco’s Modified Eagle Medium (DMEM) with 1g/l D-glucose (Sartorius, 01-050-1A), supplemented with fetal bovine serum (Biological Industries ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 mL Tris buffer (50mM, pH 7.6) and afterwards lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Cell Biology 2024Quote: ... One hundred microliters of the combined LIVE/DEAD® assay reagent was added to each well and incubated for 45 min before quantification of percentage of red and green cells using the IncuCyte® ZOOM (Sartorius) system and its proprietary ZOOM 2016A software.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Green fluorescence was immediately followed every 90 or 120 minutes with an Incucyte® S3 Live-Cell Analysis system (Sartorius, Germany). Images were analyzed with Incucyte software ...
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bioRxiv - Molecular Biology 2023Quote: ... For the Incucyte assay, THP-1 or Jeko-1 cells were transduced with IncuCyte NucLight Red Lentivirus Reagent (EF-1 Alpha, Puro) (Sartorius, USA). All cell lines were repeatedly tested for the presence of mycoplasma contamination with a MycoReport Mycoplasma Detection Kit (Evrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Phase confluence cell images were obtained using a 10× objective lens every 6 hours for 168 hours and the average confluence of each well was calculated with the IncuCyte S3 Live Cell Analysis System (Sartorius, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...
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bioRxiv - Cancer Biology 2024Quote: The growth and viability of PDAC organoids and PBMCs organoid co-cultures were monitored with the live-cell imaging system IncucyteR S3 (Sartorius, Germany). The Incucyte S3 system from Sartorius proves to be a valuable asset for observing the time-lapse behavior of organoids in response to a particular treatment ...
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bioRxiv - Cancer Biology 2024Quote: ... and only spheres ≥50 µm in diameter were counted using the IncuCyte® SX1 live cell imaging system (Sartorius, Göttingen, Germany). Frequencies of sphere-forming cells in the experimental groups were calculated and compared using ELDA software [18].
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bioRxiv - Microbiology 2021Quote: ... The cell-free viral fraction was concentrated to about 50 mL with tangential flow filtration (50R VivaFlow, Sartorius Lab instruments, Goettingen, Germany) and used as inoculum in a multi-step viral enrichment experiment followed by Dilution-to-Extinction purification as described previously (15) ...
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bioRxiv - Bioengineering 2020Quote: ... 35 mm dishes were imaged using a phase-contrast live-cell imaging system and a 4x objective (IncuCyte Zoom, Sartorius, Gottingen, Germany).
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 cells with nuclear-localized GFP (4T1-nucGFP) were established by introducing NucLight Green Lentivirus Reagent (Essen BioScience by Sartorius, Goettingen, Germany) under the selection of 2 µg/ml puromycin ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated at 37°C with 5% CO2 during 24h in the IncuCyte S3 live-cell imaging and analysis system (Essen BioScience, Sartorius, Göttingen, Germany). Images were acquired using a 10X objective and 440-480 nm Excitation / 504-544 nm Emission filters ...
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bioRxiv - Cancer Biology 2022Quote: ... Confluence was assessed over time using an IncuCyte® Zoom Live Cell Imaging instrument with data analyzed using IncuCyte® Analysis Software (Sartorius) in two-hour intervals.
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bioRxiv - Synthetic Biology 2022Quote: ... Cell dry weight (CDW) measurements were performed by filtrating 1 mL of cell culture through a weighed 0.45 μm filter membrane (Sartorius Biolab, Gottingen, Germany) and weighting the filter after microwaving it for 15 min and letting it dry in a desiccator for 24h.
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bioRxiv - Microbiology 2022Quote: ... RKO cells were then incubated for 72 hrs at 37°C in an IncuCyte S3 live imaging incubator (Sartorius and Essun BioScience). Each well was imaged in five locations with a 10X objective every two hrs for a total of 72 hrs (Camera ...
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bioRxiv - Cancer Biology 2022Quote: ... The percentage of confluent cells was monitored for 125 hours using a high-definition automated imaging system (IncuCyte®; Sartorius, Goettingen, Germany). Data were evaluated using ANOVA and presented as mean ± standard errors ...
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bioRxiv - Immunology 2023Quote: ... Red nuclei as a measurement for the total cell number and IE1 positive nuclei were counted with the IncuCyte S3 (Essen BioSciences, Sartorius, Göttingen, Germany) and the ratio of infected cells was calculated.
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bioRxiv - Bioengineering 2023Quote: Real-time monitoring of PEO4 cell growth within the hydrogel multispheroids and in the absence of treatment was conducted using an IncuCyte S3 Live Cell Analysis System (Sartorius, Gottingen, Germany). Three cell densities (2000 ...
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bioRxiv - Immunology 2023Quote: ... CD14+-cells were immunomagnetically isolated from PBMCs and incubated with fluorescently labelleled Staphyloccocus aureus and Pseudomonas aeruginosa bioparticles in an Incucyte SX1 life cell imagining incubator (Sartorius, Göttingen, Germany) over 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with a chimeric lectin-library as previously described (38) and screened on IQue Screener Plus (IntelliCyt/Sartorius, Göttingen, DE). All data was analyzed by FlowJo (BD ...
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bioRxiv - Bioengineering 2024Quote: ... The plate was centrifuged for 5 minutes at 100g and then placed in the IncuCyte S3 Live-Cell Analysis System (Sartorius, G_ttingen, Germany) and stored at 37°C ...
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bioRxiv - Genetics 2021Quote: ... according to manufacturer’s protocol and cultured at 1×105 cells/mL in CellGenix GMP SCGM serum-free base media (Sartorius CellGenix GmbH, Freiburg, Germany) supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 150 transparent (NTC-sgRNA) MCF7-dcas-9KRAB cells were seeded per well in a 96 wells ImageLock plate (Sartorius – cat no 4379) both in the presence and absence of oestradiol (Complete medium with 10% FCS +/- 17-ß Oestradiol 1x10-8 M (Sigma Aldrich – cat no E-060) ...
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bioRxiv - Immunology 2022Quote: Virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators with a size exclusion of 100 kDa (Sartorius Stedim Biotech) in order to remove cytokines of lower molecular weight ...