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Citations for BioLegend :
151 - 200 of 1129 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... and the cell lysates were assessed on a human IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with biotinylated detection antibodies against IL-12 (Biolegend Cat#505302, clone C17.8) and IL-1β (Biolegend Cat#515801 ...
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bioRxiv - Cancer Biology 2023Quote: ... Vcam1 detection: APC anti-mouse CD106 (Vcam1) antibody (Cat. No. 105717, BioLegend, San Diego, CA, USA). Cell proliferation ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained for 15 minutes at RT with 100 μL of Zombie UV dye (BioLegend) diluted 1:500 in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... washed in PBS and incubated for 1h at RT with anti-IL2 JES6-5H4 (APC, Biolegend), anti-IFNγ XMG1.2 (BV421 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature ...
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bioRxiv - Pathology 2022Quote: ... Cells were then washed 3 times with cell staining buffer (#420201, BioLegend) followed by resuspension of the cells in DAPI containing FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... then washed 6 times followed by incubating with TMB substrate (Biolegend/421101) and stop solution ...
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bioRxiv - Cancer Biology 2024Quote: MM.1S cells were washed three times with Cell Staining Buffer (BioLegend) and then fixed in 1x Fixation Buffer (4% paraformaldehyde ...
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bioRxiv - Microbiology 2020Quote: ... Primary detection of TRX-tagged YpTop1 was with primary murine monoclonal anti-TRX (BioLegend #658902, 1μg/ml), ...
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bioRxiv - Biochemistry 2019Quote: ... The final dilutions used for the detection Abs were 1:1000 for PE-conjugated anti-FLAG (Biolegend) and PE-conjugated anti-1D4 ...
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bioRxiv - Cell Biology 2021Quote: ... For CD9 detection we used Alexa Fluor® 647-conjugated anti-CD9 anti-body (BioLegend, Cat#124810) at a final concentration of 2.5μg/ml and we run in parallel samples with IgG isotype control (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and detection of indicated proteins was carried out using the following antibodies: Eos (W15169A; BioLegend, 1:500), pY-STAT5 (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... The final dilutions used for the detection Abs were 1:1,000 for PE-conjugated anti-FLAG (BioLegend) and PE-conjugated anti-1D4 ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 100μl PBS per tube with 1μl/100μl Zombie Aqua Fixable Live Cell Detection reagent (BioLegend). Cells were fixed in 250 µl of Fixation/Permeabilization solution (BD Biosciences ICCS Kit ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated for 30 min at RT with primary antibodies (Biolegend 844301, anti-Cas9 Clone 7A9) diluted at 1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... ADT and HTO additive primers were added to the RT reaction according to the manufacturer’s instructions (BioLegend). RNA and ADT/HTO libraries were separated using SPRIselect reagent and libraries were prepared separately according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Following a wash, cells were incubated (RT, 10 min) with Rhesus Fc Receptor Binding Polyclonal Antibody (Biolegend). Desired staining antibodies were added directly to this mixture at indicated dilutions (Supplemental Table 27 ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at RT after adding Human TruStain FcX (1:200 dilution, Biolegend, San Diego, CA) for 5 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were thawed on ice prior to detection and analysis using LEGENDplex human hematopoietic stem cell panel (BioLegend), according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with IFNγ detection antibody and antibodies against NKp80 (anti-NKp80-APC, Biolegend, San Diego, CA), CD3 (anti-CD3-Cy7APC ...
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bioRxiv - Microbiology 2019Quote: ... The upper size limit detection threshold was set by fluorescent rainbow particles with mid-range intensity and size 3.0-3.4 µm (Biolegend) resuspended in HBSS ...
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bioRxiv - Immunology 2021Quote: ... 2.5×105−1×106 cells for each condition resuspended in 100μl PBS per tube with 1μl/100 μl Zombie Aqua Fixable Live Cell Detection reagent (Biolegend). Tubes were incubated for 15 minutes in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 2.5×105-1×106 cells for each condition resuspended in 100μl PBS per tube with 1μl/100 μl Zombie Aqua Fixable Live Cell Detection reagent (BioLegend). Tubes were incubated for 15min in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and detection antibodies (biotinylated mouse anti-IgG1, anti-IgG2a or anti-IgE from BioLegend) were added and incubated at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2019Quote: ... and subsequently washed twice prior to incubation in the dark at RT with suitably matched secondary antibodies (Biolegend 406605 ...
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bioRxiv - Cancer Biology 2022Quote: ... for one hour at RT and stained with the following antibodies: Alexa Fluor 488 anti-NESTIN (#656812, Biolegend), Alexa Fluor 555 anti-S100B (#ab274881 ...
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bioRxiv - Neuroscience 2022Quote: ... before washing and transferring to PBS and treating with Zombie NIR (BioLegend, 1:100, 15 min at RT) to label dead cells ...
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bioRxiv - Immunology 2022Quote: ... Cell surface staining was performed at RT for 15 minutes with a panel of antibodies purchased from Biolegend, BD Biosciences or Miltenyi (Table S13) ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1 hour at RT prior to developing with 3,3’,5,5’-tetramethylbenzidine (TMB) substrate solution (BioLegend). The reaction was stopped by adding 0.1M sulfuric acid and the optical density was determined at 450 nm using a SpectraMax iD5 spectrophotometer.
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bioRxiv - Developmental Biology 2023Quote: ... then for 1 hour at room temperature (RT) with biotinylated secondary antibodies and finally with fluorochrome-labeled (BioLegend) or peroxidase-labeled streptavidin (Beckman Coulter) ...
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bioRxiv - Immunology 2024Quote: ... Erythrocytes (RBC) were lysed for 10 min at room temperature (RT) using 1x RBC Lysis/Fixation solution (Biolegend). Samples were washed with phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed 1 time then Zombie Green (Biolegend, Catalog #423111, 1:1000) was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... ATCC MM.1S cells were washed three times with cell staining buffer (BioLegend) and stained with 5 μM MitoSOXTM Red (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... This suspension was incubated with an equal volume of 71.5 μg/mL of oligo-barcoded anti-VEGF-A detection antibody (Biolegend) for 30 minutes at 37°C with gentle vortexing every 10 minutes before washing excess antibody with one high-dilution wash (>100x nanovial volume) ...
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bioRxiv - Immunology 2023Quote: ... IFNγ ELISAs used the MD-1 clone for coating and a biotinylated 4S.B3 clone for detection prior to visualization with a streptavidin-HRP antibody (BioLegend).
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bioRxiv - Microbiology 2023Quote: ... Detection of IgE from mouse serum was performed by ELISA using ELISA MAX™ Standard Set Mouse IgE (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... the cells were fixed for 1 h at RT using the True-Nuclear transcription Factor Buffer Set (BioLegend, 424401). Following the fixation ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated in 5% dried non-fat milk powder dissolved in TBS-Tween 0,1% (TBST) for 1 h at room temperature (RT) and subsequently incubated with primary anti-HA antibody (Biolegend) followed by incubation by secondary goat HRP-conjugated anti-mouse IgG (Promega) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... primary antibody 4G8 was incubated overnight at a 1:1000 dilution at room temperature (RT) (Biolegend, San Diego, CA). HRP-linked secondary goat anti-mouse antibody at a 1:1000 dilution (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with primary antibodies for 1h at RT with shaking (1:2000 anti-filaggrin clone Poly19058, Biolegend; 1:2000 anti-β-actin ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 h at RT and stained with two staining panels: (1) anti-CD3 (clone UCHT1; #300415, BioLegend, USA) and anti-CD14 (clone HCD14 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Bioengineering 2021Quote: ... cells were washed three times and stained against αCD8 mAb (clone 53-6.7, BioLegend) at 4°C for 30 min ...
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bioRxiv - Immunology 2022Quote: ... After the plate was washed five times and a 3,3′,5,5′-Tetramethylbenzidine solution (Biolegend) was prepared and incubated for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed 3 times with PBS and counter stained with DRAQ5 (BioLegend) to stain DNA for 10 min ...
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bioRxiv - Immunology 2022Quote: ... The nanoparticles were washed again and finally resuspended in the 100 µL of assay buffer containing fluorescently labeled AlexaFlora568-detection antibody for IFNγ (Biolegend).
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bioRxiv - Bioengineering 2022Quote: ... at 0.5 μL/well for 5 hours and washed with PBS for detection of intracellular IFNγ using anti-hIFNγ APC antibody (Biolegend). The frequency of IFNγ positive cells was analyzed by flow cytometry (CytoFLEX II ...