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Citations for BioLegend :
201 - 250 of 1126 citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cells suspensions of 100 μL were incubated for 15 min at RT with 2 μL Human TruStain FcX (Biolegend, 422301) to prevent nonspecific Fc receptor binding ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then blocked (PBS-0.25% Triton X-100+ 1% BSA (bovine serum albumin)) for 1h at RT and then primary antibodies (anti-βIII tubulin (Tuj1, 1:1000, BioLegend), anti-NFH (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were blocked with 5% bovine serum albumin (BSA) and 5% goat serum for 1hr at RT and incubated with primary antibodies (anti-βIII-tubulin antibody [BioLegend]) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with LIVE/DEAD Far Red viability for 20 minutes at RT and then with anti-CD32 (FITC, Biolegend) and anti-CD3 (PerCP ...
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bioRxiv - Neuroscience 2019Quote: ... slides were incubated for 1.5 hrs at RT with a cocktail of secondary fluorescence-conjugated antibody Alexa488 and DAPI (4’,6-diaidino-2-phenylindole, dilactate; BioLegend) to visualize nuclei ...
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bioRxiv - Immunology 2020Quote: ... for 30’ at RT in PBS containing 2% FCS and 1 mM EDTA prior to secondary staining with streptavidin-BrilliantViolet 510 (Biolegend) for 15’ on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11, PercPcy55, 1/300, Biolegend). Tumor cells (for RNA-seq ...
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bioRxiv - Immunology 2021Quote: ... for 15 min at RT and stained intracellularly with Fix/Perm Buffer B and antibodies for TNF (PE-Cy7, clone MAb11, BioLegend), IFN-γ (BV711 ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation for 1h at RT in a 1:1000 dilution in blocking buffer of primary Ab (purified anti-Filaggrin polyclonal Ab, clone Poly19058, Biolegend). After washing ...
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bioRxiv - Neuroscience 2021Quote: ... Except for immunohistochemistry for SOX-9 in which a β-gal staining was performed at 37°C for 3h followed by fixation with PFA 4% for 30 min at RT and incubation with unmasking antigen solution (927901; Biolegend). Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40 ...
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bioRxiv - Immunology 2022Quote: ... The permeabilised cells were then intracellularly stained for 1 hour at room temperature (RT) with anti-STAT6Tyr641 Alexa Fluor 647 (A15137E; Biolegend) and anti-NFκB p65Ser536 Alexa Fluor 488 (93H1 ...
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bioRxiv - Cell Biology 2023Quote: ... washed with PBS 1% FBS and incubated 10 mins at RT with Human TruStain FcX and True-Stain Monocyte blocker (Biolegend). Antibody mix containing CD14-APCFire750 ...
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bioRxiv - Cell Biology 2023Quote: ... iMACs were then washed with PBS plus 2% FBS and incubated for 15 min at RT with 50 ul/mL Human TruStain FcX Blocking solution (BioLegend) in PBS + 2% FBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized using BD Fix/Perm for 20 min at RT and stained intracellularly with anti-IFN-γ (1:100 dilution; clone XMG1.2, Biolegend), anti-TNF-α (1:100 dilution ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with a premixed cocktail of the indicated tetramers at a final concentration of 10 nM for 1 hour at RT in the presence of anti-mouse CD16/32 antibody (clone 93, BioLegend). SLO preparations were magnetically enriched for tetramer-specific cells as previously described64 while lung samples were magnetically enriched for CD90.2 expression (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed with 4% PFA for 10 min at RT followed by permeabilization with the True-Phos Perm buffer (Biolegend) for 20 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with FACs buffer (1× PBS, 1% FBS) and fixed at RT for 20 min with Intracellular Staining Fixation Buffer (BioLegend). Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times and incubated ∼7min with 50 µl of TMB substrate (BioLegend). The reaction was stopped with 25µl of 1M phosphoric acid (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were washed three times with TBST and incubated with HRP-conjugated secondary antibodies (Biolegend) for 1h at RT ...
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bioRxiv - Immunology 2020Quote: ... samples were analyzed at the same time with the LEGENDplexTM Mouse Inflammation Panel (Biolegend, USA). The assay was performed following manufacturer’s instructions for measuring serum or plasma samples using a V-bottom plate ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed five times with PBST and developed with tetramethylbenzidine substrate (Biolegend, 421101). After 20 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 0.1% BSA) before secondary detection with 1:100 dilutions of APC- conjugated mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for detection of MDSCs contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106), a rat anti-mouse Ly-6G/Ly-6C (Gr1 ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for CTC detection of human CTCs in animal models contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106) and mouse anti-human HLA conjugated to AlexaFluor 647 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions) were incubated with 10 nM of a second CD98hc antibody for detection (RL388, rat anti-mouse antibody; BioLegend, 128202). Non-treated cells were also incubated with the TfR-1 (R17 ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells are permeabilized with 0.1% Triton-X 100 for 10min at RT and stained with anti-vimentin (1:200, Biolegend, CA, USA) for 30 min in PBS containing 5% normal goat serum (RT) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked using DMEM/FBS media for 1 hour at RT followed by incubation with 1:1000 dilution mouse anti-HA (Biolegend, 901514) and rabbit anti-PCNA (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... BSA/0.1% (v/v) TritonX-100 in PBS for 30 min at RT and stained for actin with Alexa594-conjugated phalloidin (BioLegend, USA) diluted in 0.3% (w/v ...
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bioRxiv - Pathology 2022Quote: ... Cells were washed (PBS; twice), centrifuged (300g; 5min.; 4°C) and stained (10min., RT) with Streptavidin (Alexa Fluor® 647-conjugated, Biolegend, #405237 ...
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bioRxiv - Bioengineering 2022Quote: ... Single cell suspensions of pericytes were stained for 30 minutes at RT using fluorescence-labelled antibodies for pericyte surface markers (488-NG2 from Invitrogen, APC PDGFR-β and APC CD105 from Biolegend) and DAPI for cell viability ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were blocked with 5% non-fat milk at room temperature (RT) for 1 h and probed with primary RpoC (β prime subunit of RNAP) antibodies at 1:2000 dilutions (Biolegend) at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... Slides were incubated in blocking buffer for 30 minutes at RT prior to staining for epithelial cells by anti-mouse IgG2a CD326/Ep-CAM conjugated to Alexa-Fluor 488 (Biolegend, 118210) for 1 hour at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Cancer Biology 2023Quote: ... a fixation/permeabilization step was performed by incubating the cells for 20 min at RT using a Cyto-Fast Fix/Perm Buffer set (#426803, BioLegend, USA). Intracellular antibody staining was then performed for 25 min at RT ...
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bioRxiv - Immunology 2023Quote: ... All labelling was carried out at room temperature (RT) for 10mins protected from light with antibodies diluted in cell staining buffer (Biolegend; 420201). For AnnexinV staining ...
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bioRxiv - Immunology 2020Quote: ... plates were washed five times with 200µL freshly prepared 0.05% Tween-based ELISA Wash Buffer (BioLegend) and a final time in 200μL phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... after which time cells were stained with 5 μg/ml CD8-APC (clone SK1; Biolegend, UK), harvested and CSFE dilution measured by flow cytometry ...
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bioRxiv - Microbiology 2023Quote: ... Cell were then washed two times with intracellular staining permeabilization wash buffer (BioLegend, catalog no. 421002) and stained with previously prepared antibody cocktail for intracellular staining ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with cell staining buffer and resuspended in Cell Staining Buffer (BioLegend) prior to flow cytometry using a MACSQuant Analyzer (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2022Quote: ... For immune cells detections the tumoroids sections also were incubated overnight at 4°C with anti-B220 (Biolegend 103224, APC-cy7 conjugated) and anti-cd11b (Biolegend 101208 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 10 min at RT in 1.0 μg anti-mouse CD16/32 antibody (BioLegend, San Diego, CA, USA) containing 500 μl FACS buffer for Fc receptor blocking ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
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bioRxiv - Pathology 2022Quote: ... blood was harvested at the indicated time post infection and treated with 1x RBC lysis solution (BioLegend). Murine Fc receptors were blocked using anti-mouse CD16/CD32 (FcBlock clone 2.4G2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plates were washed three times with PBS and blocked with Cell Staining Buffer (BioLegend, San Diego, CA). Plates were then incubated with rabbit-anti-LC3B (Cell Signaling Technologies ...
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bioRxiv - Physiology 2019Quote: ... and SLAMF6 expression was tested at the indicated time points using anti-SLAMF6 (Biolegend, clone 330-AJ).