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Citations for BioLegend :
251 - 300 of 1126 citations for rno mir 101a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 2 times in 1x Perm/Wash buffer and resuspended in Cell Staining Buffer (Biolegend) prior to flow cytometry analysis ...
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bioRxiv - Immunology 2021Quote: ... cells were restimulated with cognate antigens for three days and then supernatants were collected for IL-17A and IFNγ detection with ELISA (Biolegend, San Diego, CA). For determination of IL-17A+ and IFNγ+ CD45.1+ OT-II CD4+ T cell percentages ...
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bioRxiv - Microbiology 2021Quote: ... incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... tumor cell suspensions were incubated 10 min at RT with Fc block (Leico; C381) and a viability dye (Biolegend; Zombie Aqua (ref # 423102) or Zombie NIR (ref # 423106) ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101; TNFα ELISA kit, BioLegend # 430204) or using LEGENDplex™ Human CD8/NK Panel Kit (Cat# 741186) ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Sections were washed three times with PBS and a secondary anti-rat IgG AlexaFluor488 antibody (BioLegend, London, UK) was used for detection ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were rinsed three times in IF solution and incubated with 300 nM DAPI (BioLegend, San Diego, CA) in deionized water for 15 minutes at room temperature protected from light ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were again washed 3 times before adding 50 µL per well of HRP Streptavidin (#405210, BioLegend, USA) diluted 1:2000 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... kidneys were washed three times for 1 hr at room temperature in Ce3D™ Wash Buffer (427710, Biolegend), then cut in half and transferred to Ce3D™ Permeabilization/Blocking Buffer (48 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... All compensation was performed at the time of acquisition in Diva software by using compensation beads (BioLegend, A10497) for single-color staining and SVCs for negative staining and fluorescence (DAPI and tdTomato).
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Bioengineering 2021Quote: ... IL6 was quantified using an ELISA developed from an antibody pair (biotin anti-rat IL6 detection antibody, cat. #517703, and purified anti-rat IL6 coating antibody, cat. #517701, Biolegend, San Diego, CA, USA). For this ELISA ...
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bioRxiv - Immunology 2022Quote: ... 4 °C) cells were stained with the following conjugated antibodies for 20 min at RT in the dark: CD3-APC (Biolegend, clone REA613, 1:200), CD11b-APC-Cy7 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... washed once with PBS and stained for 30 min at RT in the dark with anti-CD4 antibody (PerCP-Cy5.5, Biolegend #317428, 1:50 in PBS) and eBioscience Fixable viability dye 780 (ThermoFisher Scientific #65-0865-14 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized with 0.1% Triton X-100 in PBS (5 min at RT) and immunolabeled with anti-HA (Biolegend, 901501; 1:500 in PBS) for 1 hr at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed three times in FACS buffer and resuspended in FACS buffer containing 3 μg/ml DAPI (BioLegend). Flow cytometric analysis was done on a BD LSRFortessa analyzer and sorting was done on a BD FACSAria1 machine.
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bioRxiv - Immunology 2022Quote: ... The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each with PBS plus 0.05% Tween-20 and then blocked with 1x Assay Diluent A (BioLegend) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Cells were resuspended with the intracellular cocktail of antibodies for 45 minutes on ice and were washed and incubated for 20 minutes at RT with Ir-intercalator (Biolegend CNS, San Diego, CA, USA), prepared at a dilution of 1:500 in 2% fresh PFA ...
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bioRxiv - Cancer Biology 2021Quote: ... c-Kit (BioLegend), EpCam (G8.8 ...
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bioRxiv - Cell Biology 2020Quote: ... After staining BM was washed two times with cell stain buffer followed by a second incubation step with PE-conjugated Streptavidin (405204, Biolegend) for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed 3 times in TBST and incubated in TBST containing 5% non-fat dry milk and 1:1000 mouse anti-His (Biolegend) overnight ...
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bioRxiv - Immunology 2021Quote: ... Slides were again washed 3 times with 1x TBS and stained with FITC–anti-IgD mAb (clone 11-26c.2a; 405713, BioLegend), PE goat-anti-mouse-IgM mAb (406507 ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed four times with PBS-T and developed with 100 µL/well TMB High Sensitivity substrate solution (BioLegend, UK). After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed three times in ice cold blocking buffer and re-suspended in blocking buffer containing 0.1 μg/ml DAPI (Biolegend, USA).
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bioRxiv - Pathology 2020Quote: ... were then stimulated for the indicated time points (from 0 to 120 minutes) before being treated with fixative buffer (Biolegend) according to the manufacturer recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with PBS for three times and incubated with rat anti-mouse IgG2a conjugated with APC (407109, BioLegend) for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and developed with 100 µl/well TMB High Sensitivity substrate solution (Biolegend). After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and developed with 100 µl/well TMB high sensitivity substrate solution (Biolegend). After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2022Quote: ... The membrane was washed three times with TBS-T for 10 min and subjected to the HRP-conjugated secondary goat anti-mouse antibody (Biolegend) in a solution of 3% non-fat milk in TBS-T for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed 2 times then resuspended in 100 μL wash buffer with 0.1 μg/mL streptavidin-PE (Biolegend 405203) and incubated on ice for 15 min ...
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bioRxiv - Immunology 2023Quote: ... cells were hashtagged with mouse TotalSeqA DNA-barcoded hashtag antibodies at the same time as staining with fluorophore-conjugated antibodies (BioLegend). Cells were sorted into RPMI + 5% FCS in DNA Lo-Bind tubes (Eppendorf ...
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bioRxiv - Immunology 2023Quote: ... cells were hashtagged with mouse TotalSeqA DNA-barcoded hashtag antibodies at the same time as staining with fluorophore-conjugated antibodies (BioLegend). Hashtags used in each experiment are provided in Table S1 ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 5 times in 150 µL of PBST and incubated with 100 µL of streptavidin conjugated to horseradish peroxidase (HRP, BioLegend) diluted 1:2000 in PBS for 1 hour at RT ...
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bioRxiv - Immunology 2024Quote: ... Wells were washed 5 times with 200 µL PBST and streptavidin-conjugated to horseradish peroxidase was added (1:2000 in PBS, BioLegend) for 30 minutes at RT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed two times with PBS-Tw and 3,3’,5,5’-tetramethylbenzidine peroxidase (TMB) substrate (BioLegend; San Diego, CA, USA) was added ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101 ...
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bioRxiv - Immunology 2020Quote: ... according to manufacturer’s instructions kits (ELISA MAX™ Deluxe Kits from Biolegend). The detection limit was 15.6 pg/ml.
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bioRxiv - Cancer Biology 2021Quote: Cells were harvested at the indicated times and co-stained with Annexin-V-FITC and propidium iodide following manufacturer instructions (BioLegend, #640914). To assess active Caspase-3 ...
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bioRxiv - Immunology 2022Quote: ... the plate was washed five times with PBST and incubated with anti-mouse secondary antibody with minimal cross reactivity with human IgG (Biolegend, 405306). The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times with PBS-T (PBS with 0.1% Tween-20) and 50μl of mouse IgG-specific secondary antibody (BioLegend #405306, 1:10,000) diluted in dilution solution added to each well ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were processed at the same time using the purified mouse monoclonal anti-beta-amyloid 1-16 antibody (6E10) (Biolegend, 803001) at 1/500 for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times with PBS-T (PBS with 0.1% Tween-20) and 50μl of mouse IgG-specific secondary antibody (BioLegend #405306, 1:10,000) diluted in dilution solution added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Bioengineering 2023Quote: 50 μm streptavidin-coated nanovials were reconstituted at a five-time dilution in Washing Buffer containing 270 nM (40 μg/mL) of biotinylated anti-CD63 antibody (Biolegend, 353018). Nanovials were incubated with antibodies for 30 minutes at room temperature on a rotator and washed three times as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed in FACS buffer three times and fixed overnight in 200 µL True Nuclear fixation buffer (Biolegend #73158, #71360) at 4°C ...