Labshake search
Citations for BioLegend :
151 - 200 of 282 citations for mmu mir 378c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Intracellular staining was either performed using the True-Nuclear Transcription Factor Buffer Set (Biolegend, San Diego, CA, Cat # 424401) or CytoFix Fixation buffer / FACS Permeabilizing solution 2 (BD Biosciences ...
-
bioRxiv - Immunology 2020Quote: ... and Il6) in treated PBMC supernatants were quantified by ELISA using the ELISA MAX Deluxe Set Human CCL2 (Biolegend), ELISA MAX Deluxe Set Human IL8 (Biolegend) ...
-
bioRxiv - Microbiology 2022Quote: ... and TNF-α was measured by enzyme-linked immunosorbent assay (ELISA) per manufacture’s instruction (Biolegend, ELISA MAX Deluxe Set) and CXCL-1/GRO-α was measured using a R&D systems DueSet ELISA kit.
-
bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
-
bioRxiv - Immunology 2023Quote: ... The samples were incubated for 1 hour with fixation buffer from True-Nuclear™ Transcription Factor Buffer Set (Biolegend) and washed with permeabilization buffer from the same set ...
-
bioRxiv - Microbiology 2023Quote: ... Detection of IgE from mouse serum was performed by ELISA using ELISA MAX™ Standard Set Mouse IgE (Biolegend) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... The supernatants were obtained and IFN-γ and TNF-α concentrations were quantified using ELISA MAX Standard Sets (Biolegend; cat.# 430801 for IFN-γ ...
-
bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was performed according to manufacturer’s instructions for staining intracellular antigens (eBioscience) or using the True-Nuclear Transcription Factor Buffer Set (BioLegend). Flow cytometric analysis was performed using a FACS FORTESSA and FlowJo softwares (Tree Star ...
-
bioRxiv - Immunology 2023Quote: ... and then single-cell suspensions were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Biolegend, #426803) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... FACS gates were set based on CD45 (Alexa Fluor® 594 anti-CD45, 1:20, Biolegend, #368520, clone 2D1), SYTOX Blue and DRAQ5 intensities to isolate live CD45+ and CD45-populations as shown in Supplementary Fig ...
-
bioRxiv - Immunology 2024Quote: ... The cells were re-suspended in fixation and permeabilization buffers using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... and IL-1β concentrations were determined with the ELISA MAX™ Deluxe Set Human IL-1β (BioLegend, Cat# 437004) in 96-well half area Costar assay plates (Corning ...
-
bioRxiv - Microbiology 2021Quote: ... and incubated for at least 60 min at RT (BioLegend, San Diego, CA, USA). Slides were washed 2 times in 0.5% BSA/PBS and once in PBS at RT ...
-
bioRxiv - Immunology 2022Quote: ... we lysed red blood cells using RBC Lysis Buffer for 5min at RT (BioLegend). RBC Lysis Buffer was quenched with MACS buffer and washed once ...
-
bioRxiv - Immunology 2023Quote: ... and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend). Intracellular staining was performed for cytokines using antibodies for IL-5 ...
-
bioRxiv - Cell Biology 2023Quote: ... and DRAQ5 (1ng/mL, incubation 5 minutes at RT; BioLegend, San Diego, CA, USA). Samples were measured using a FACS Canto II (BD Biosciences ...
-
bioRxiv - Cancer Biology 2024Quote: ... blocked for 1 hour at RT with 1x ELISA dilutant buffer (Biolegend Cat# 421203), then washed twice with 1x wash buffer ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then stained with the following antibodies and stains (in multiple sets to prevent fluorophore overlap): CD4-FITC (BioLegend), CD8-PECy5 (BioLegend) ...
-
bioRxiv - Immunology 2021Quote: ... Intracellular staining for FOXP3 and HELIOS was performed using FOXP3 Fix/Perm Buffer Set (BioLegend®, San Diego, CA USA). All antibodies were used at manufacturer-recommended concentrations ...
-
bioRxiv - Immunology 2019Quote: ... Cells were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set (eBiosciene) and afterwards intracellularly stained with CD3-AlexaFluor700 and Ki-67-BV711 (clone Ki-67, Biolegend). In-depth analysis of CD27−IgD− B cell phenotype was performed with Fixable viability stain 780 or aqua viability dye V510 ...
-
bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies to detect IRF4 (3E4) and PRDM1 (5E7) was performed using True-Nuclear Transcription Factor Buffer Set (Biolegend). For mitochondrial stains ...
-
bioRxiv - Molecular Biology 2022Quote: ... or DDX39B-depleted (Sh3 and Sh5) MT-2 cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set (BioLegend). The permeabilized cells were stained with antibodies against FOXP3 conjugated with Alexa Fluor 647 (AB_2539534 ...
-
Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Levels of IFN-γ in supernatants from T cell clone recognition assays were measured by ELISA using the ELISA MAX Deluxe Set Human IFN-g kit (Biolegend) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
-
bioRxiv - Immunology 2021Quote: ... Fixation and permeabilization was performed with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (cat # 00-5523-00) before intracellular staining with anti-Foxp3-PE (MF-14, BioLegend) according to the manufacturer’s recommendations ...
-
bioRxiv - Immunology 2022Quote: ... samples were fixed/permeabilised using the Foxp3 staining buffer set (eBioscience) prior to intracellular staining for arginase (A1exF5, eBioscience) and Ki67 (16A8, Biolegend). Anti-mouse antibodies used in this study were ...
-
bioRxiv - Microbiology 2022Quote: ... They were then washed once with the permeabilisation buffer of the staining buffer set and stained with anti-Tax-AF647 (clone LT-4) and either anti-Ki-67-PE (BioLegend) or anti-γ-H2AX-PE (clone 2F3 ...
-
bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
-
bioRxiv - Immunology 2022Quote: ... TNF and IL-2 in the supernatant of in vitro activated cells and murine IL-10 in the serum were determined using ELISA MAX Deluxe Set (BioLegend) according to manufacturer instructions.
-
bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
-
Inhaled CpG increases survival and synergizes with checkpoint inhibition in lymphangioleiomyomatosisbioRxiv - Immunology 2023Quote: ... Supernatants were diluted 1:10 with 1% BSA ELISA assay diluent and ELISA was performed according to manufacturer’s instruction (ELISA MAX™ Standard Set, Biolegend).
-
bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
-
bioRxiv - Bioengineering 2023Quote: IFNψ was measured from the supernatants of the co-culture experiments (TCE / CAR) using the colorimetric ELISA MAX Standard Set Human IFNψ kit (BioLegend) according to the manufacturer’s instruction ...
-
bioRxiv - Immunology 2023Quote: ... and additional step was performed using the Foxp3/Transcription Factor Staining Bugger Set (cat. no. 00-5523, eBioscienceTM) and FOXP3-BV421 or FOXP3-PE (Clone 150D, Biolegend) according to manufacture instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401, Biolegend) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... TNF-α production was quantified in the supernatant of RAW 264.7 cells using a sandwich ELISA Max Deluxe Set (Biolegend) according to the manufacturers protocol.
-
bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
-
bioRxiv - Immunology 2019Quote: ... at RT in the dark using the following antibodies: anti-CD3–PerCP (clone OKT3; BioLegend), anti-CD19– Brilliant Violet 510 (clone HIB19 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were collected and fixed for 20 minutes at RT with fixation buffer (Biolegend 420801), then samples were run on a BD LSRII (BD Biosciences ...
-
bioRxiv - Microbiology 2020Quote: ... cells were assessed for viability using a 30 min RT incubation with Zombie NIR (BioLegend) diluted in PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... and permeabilized for 15 minutes at RT in Intracellular Staining Perm Wash Buffer (Biolegend, 421002), before staining with Recombinant anti-Cytokeratin 8 antibody Alexa Fluor 647 (Abcam ...
-
bioRxiv - Neuroscience 2021Quote: ... intracellular staining was performed on single cell suspensions according to instructions provided by True-Nuclear™ Transcription Factor Buffer Set (Biolegend). The antibodies used were anti-IL-1β PE (ThermoFisher) ...
-
bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
-
bioRxiv - Developmental Biology 2020Quote: Cells obtained from embryos and stained with Epha1 antibodies as described above were then washed twice with PBS/10% DS and processed for staining using True-NuclearTM transcription factor Buffer Set (BioLegend #424401) according to the manufacturer’s instructions and following the protocol described in (Aires et al. ...
-
bioRxiv - Immunology 2021Quote: ... The purity of the sorted Treg subsets was assessed by staining the cells with FoxP3-APC mAb using the FoxP3 Fix/Perm Buffer Set for nuclear staining (Biolegend, USA). Over 95% of purity was detected in all cases analyzed ...
-
bioRxiv - Genomics 2022Quote: ... supernatants were removed and cells fixed according to the manufacturer’s instructions utilizing the True-Nuclear™ Transcription Factor Buffer Set (Biolegend, 424401). Intracellular staining was performed according to manufacturer’s instructions before FACS analysis was carried out ...
-
bioRxiv - Microbiology 2022Quote: The amount of TNFα secreted in the supernatants from cell culture and cell lysate was determined by ELISA MAX™ Deluxe Set Mouse TNFα kit (BioLegend) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: Global protein concentration of the BV2 cells post treatment was assessed via flow cytometry using intracellular protein staining using the True-Nuclear Transcription Factor Buffer Set (Biolegend # 424401). Cells were incubated with primary antibodies for 30 minutes - 1:100 Acetyl-Histone H3 (Lys27 ...
-
bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
-
bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...