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Citations for BioLegend :
151 - 200 of 284 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Secreted cytokines were measured with ELISA MAX Standard Set Mouse or Human kits according to the manufacturer’s protocol (BioLegend). Cellular viability was determined by resuspending cells in cRPMI with 0.1mM resazurin (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... BMDMs supernatant and intracellular IL-12p70 were quantified by using ELISA MAXTM Deluxe Set Mouse IL-12 (p70) (Biolegend).
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bioRxiv - Immunology 2020Quote: ... Intracellular staining was either performed using the True-Nuclear Transcription Factor Buffer Set (Biolegend, San Diego, CA, Cat # 424401) or CytoFix Fixation buffer / FACS Permeabilizing solution 2 (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... and Il6) in treated PBMC supernatants were quantified by ELISA using the ELISA MAX Deluxe Set Human CCL2 (Biolegend), ELISA MAX Deluxe Set Human IL8 (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α was measured by enzyme-linked immunosorbent assay (ELISA) per manufacture’s instruction (Biolegend, ELISA MAX Deluxe Set) and CXCL-1/GRO-α was measured using a R&D systems DueSet ELISA kit.
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bioRxiv - Immunology 2023Quote: ... and then single-cell suspensions were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Biolegend, #426803) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Immunology 2023Quote: ... The samples were incubated for 1 hour with fixation buffer from True-Nuclear™ Transcription Factor Buffer Set (Biolegend) and washed with permeabilization buffer from the same set ...
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bioRxiv - Immunology 2023Quote: ... The supernatants were obtained and IFN-γ and TNF-α concentrations were quantified using ELISA MAX Standard Sets (Biolegend; cat.# 430801 for IFN-γ ...
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bioRxiv - Cancer Biology 2023Quote: ... FACS gates were set based on CD45 (Alexa Fluor® 594 anti-CD45, 1:20, Biolegend, #368520, clone 2D1), SYTOX Blue and DRAQ5 intensities to isolate live CD45+ and CD45-populations as shown in Supplementary Fig ...
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bioRxiv - Microbiology 2023Quote: ... Detection of IgE from mouse serum was performed by ELISA using ELISA MAX™ Standard Set Mouse IgE (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was performed according to manufacturer’s instructions for staining intracellular antigens (eBioscience) or using the True-Nuclear Transcription Factor Buffer Set (BioLegend). Flow cytometric analysis was performed using a FACS FORTESSA and FlowJo softwares (Tree Star ...
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bioRxiv - Microbiology 2024Quote: ... and IL-1β concentrations were determined with the ELISA MAX™ Deluxe Set Human IL-1β (BioLegend, Cat# 437004) in 96-well half area Costar assay plates (Corning ...
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bioRxiv - Immunology 2024Quote: ... The cells were re-suspended in fixation and permeabilization buffers using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for at least 60 min at RT (BioLegend, San Diego, CA, USA). Slides were washed 2 times in 0.5% BSA/PBS and once in PBS at RT ...
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bioRxiv - Immunology 2022Quote: ... we lysed red blood cells using RBC Lysis Buffer for 5min at RT (BioLegend). RBC Lysis Buffer was quenched with MACS buffer and washed once ...
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bioRxiv - Immunology 2023Quote: ... and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend). Intracellular staining was performed for cytokines using antibodies for IL-5 ...
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bioRxiv - Cell Biology 2023Quote: ... and DRAQ5 (1ng/mL, incubation 5 minutes at RT; BioLegend, San Diego, CA, USA). Samples were measured using a FACS Canto II (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked for 1 hour at RT with 1x ELISA dilutant buffer (Biolegend Cat# 421203), then washed twice with 1x wash buffer ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with the following antibodies and stains (in multiple sets to prevent fluorophore overlap): CD4-FITC (BioLegend), CD8-PECy5 (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining for FOXP3 and HELIOS was performed using FOXP3 Fix/Perm Buffer Set (BioLegend®, San Diego, CA USA). All antibodies were used at manufacturer-recommended concentrations ...
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bioRxiv - Immunology 2019Quote: ... Cells were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set (eBiosciene) and afterwards intracellularly stained with CD3-AlexaFluor700 and Ki-67-BV711 (clone Ki-67, Biolegend). In-depth analysis of CD27−IgD− B cell phenotype was performed with Fixable viability stain 780 or aqua viability dye V510 ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies to detect IRF4 (3E4) and PRDM1 (5E7) was performed using True-Nuclear Transcription Factor Buffer Set (Biolegend). For mitochondrial stains ...
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bioRxiv - Molecular Biology 2022Quote: ... or DDX39B-depleted (Sh3 and Sh5) MT-2 cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set (BioLegend). The permeabilized cells were stained with antibodies against FOXP3 conjugated with Alexa Fluor 647 (AB_2539534 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Levels of IFN-γ in supernatants from T cell clone recognition assays were measured by ELISA using the ELISA MAX Deluxe Set Human IFN-g kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2021Quote: ... Fixation and permeabilization was performed with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (cat # 00-5523-00) before intracellular staining with anti-Foxp3-PE (MF-14, BioLegend) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... samples were fixed/permeabilised using the Foxp3 staining buffer set (eBioscience) prior to intracellular staining for arginase (A1exF5, eBioscience) and Ki67 (16A8, Biolegend). Anti-mouse antibodies used in this study were ...
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bioRxiv - Microbiology 2022Quote: ... They were then washed once with the permeabilisation buffer of the staining buffer set and stained with anti-Tax-AF647 (clone LT-4) and either anti-Ki-67-PE (BioLegend) or anti-γ-H2AX-PE (clone 2F3 ...
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bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
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bioRxiv - Immunology 2022Quote: ... TNF and IL-2 in the supernatant of in vitro activated cells and murine IL-10 in the serum were determined using ELISA MAX Deluxe Set (BioLegend) according to manufacturer instructions.
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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Inhaled CpG increases survival and synergizes with checkpoint inhibition in lymphangioleiomyomatosisbioRxiv - Immunology 2023Quote: ... Supernatants were diluted 1:10 with 1% BSA ELISA assay diluent and ELISA was performed according to manufacturer’s instruction (ELISA MAX™ Standard Set, Biolegend).
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bioRxiv - Bioengineering 2023Quote: IFNψ was measured from the supernatants of the co-culture experiments (TCE / CAR) using the colorimetric ELISA MAX Standard Set Human IFNψ kit (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... and additional step was performed using the Foxp3/Transcription Factor Staining Bugger Set (cat. no. 00-5523, eBioscienceTM) and FOXP3-BV421 or FOXP3-PE (Clone 150D, Biolegend) according to manufacture instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401, Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... TNF-α production was quantified in the supernatant of RAW 264.7 cells using a sandwich ELISA Max Deluxe Set (Biolegend) according to the manufacturers protocol.
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bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
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bioRxiv - Immunology 2019Quote: ... at RT in the dark using the following antibodies: anti-CD3–PerCP (clone OKT3; BioLegend), anti-CD19– Brilliant Violet 510 (clone HIB19 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were collected and fixed for 20 minutes at RT with fixation buffer (Biolegend 420801), then samples were run on a BD LSRII (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were assessed for viability using a 30 min RT incubation with Zombie NIR (BioLegend) diluted in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and permeabilized for 15 minutes at RT in Intracellular Staining Perm Wash Buffer (Biolegend, 421002), before staining with Recombinant anti-Cytokeratin 8 antibody Alexa Fluor 647 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... intracellular staining was performed on single cell suspensions according to instructions provided by True-Nuclear™ Transcription Factor Buffer Set (Biolegend). The antibodies used were anti-IL-1β PE (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2020Quote: Cells obtained from embryos and stained with Epha1 antibodies as described above were then washed twice with PBS/10% DS and processed for staining using True-NuclearTM transcription factor Buffer Set (BioLegend #424401) according to the manufacturer’s instructions and following the protocol described in (Aires et al. ...
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bioRxiv - Immunology 2021Quote: ... The purity of the sorted Treg subsets was assessed by staining the cells with FoxP3-APC mAb using the FoxP3 Fix/Perm Buffer Set for nuclear staining (Biolegend, USA). Over 95% of purity was detected in all cases analyzed ...
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bioRxiv - Genomics 2022Quote: ... supernatants were removed and cells fixed according to the manufacturer’s instructions utilizing the True-Nuclear™ Transcription Factor Buffer Set (Biolegend, 424401). Intracellular staining was performed according to manufacturer’s instructions before FACS analysis was carried out ...
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bioRxiv - Microbiology 2022Quote: The amount of TNFα secreted in the supernatants from cell culture and cell lysate was determined by ELISA MAX™ Deluxe Set Mouse TNFα kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Global protein concentration of the BV2 cells post treatment was assessed via flow cytometry using intracellular protein staining using the True-Nuclear Transcription Factor Buffer Set (Biolegend # 424401). Cells were incubated with primary antibodies for 30 minutes - 1:100 Acetyl-Histone H3 (Lys27 ...