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Citations for BioLegend :
151 - 200 of 5494 citations for Trefoil Factor 3 TFF3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Immunology 2020Quote: ... CD40 (3/23, BioLegend), CD115 (CSF-1R ...
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bioRxiv - Cancer Biology 2022Quote: ... LAG-3 (BioLegend, 369212) or LFA-1 (BD ...
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bioRxiv - Immunology 2022Quote: ... CD40 (3/23) (Biolegend), CD11b (M1/70) ...
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bioRxiv - Cancer Biology 2023Quote: ... Tim-3 (BV510, BioLegend), LAG-3 (BV605) ...
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bioRxiv - Bioengineering 2023Quote: ... Histone 3 (BioLegend #819411), YAP/TAZ (Santa Cruz #sc-101199) ...
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bioRxiv - Immunology 2023Quote: ... TIM-3-BV711 (Biolegend), LAG-3-APC-eFluor 780 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... TIM-3-BV711 (Biolegend), LAG-3-APC-eFluor 780 (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... caspase-3 (W20054B; Biolegend), cleaved caspase-3 (Asp175 ...
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bioRxiv - Immunology 2022Quote: ... Ab used for Regulatory T cells transcription factor intracellular staining are Foxp3 and Helios Abs (BioLegend). Flow cytometry analyses were performed using an LSRFortessa X-20 flow cytometer (BD Biosciences ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... Cells were then fixed and permeabilized using a True-Nuclear™ Transcription Factor Buffer Set (BioLegend) and stained with anti-RORγt (Q31-378 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (BioLegend 424401) following the manufacturers instructions and were then stained with anti-FOXP3 APC (Invi-trogen 17-5773-82 ...
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bioRxiv - Immunology 2023Quote: ... cell-surface stained cells were fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (BioLegend), incubated with anti-CD5L antibody for 30 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The cells were differentiated into macrophages with 50 ng/ml macrophage colony–stimulating factor (#576404, BioLegend) and maintained by incubation in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2024Quote: ... The PBMCs were then fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... VDR overexpressing SKOV-3 and ECC-1 cells were processed and stained with PDL1-BV605 antibody (Biolegend Inc, Catalog number:124321). VDR expressing SKOV-3 and ECC-1 cells ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Every 106 B cells in 100 μl solution were stained with 3 μl FITC antihuman CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μl Brilliant Violet 421 antihuman CD27 antibody (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were isolated and stimulated for 48 h with 3 μg/ml plate-bound anti-mouse CD3 antibody (100340, BioLegend, USA) and 2 μg/ml soluble anti-mouse CD28 antibody (102116 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... the cells were fixed and permeabilized with a 1× dilution of True-Nuclear Transcription Factor kit (BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Intranuclear staining of Ki-67 was performed with the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and M2 macrophages with 20ng/mL of macrophage –colony-stimulating factor (M-CSF, Cat. No. 574804, BioLegend). After 6 days ...
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bioRxiv - Immunology 2023Quote: ... After washing cells were fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) followed by intracellular staining for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized using the True-Nuclear Transcription Factor Buffer Set (BioLegend Cat. No. 424401) using the manufacturer’s protocol with overnight fixation/permeabilization.
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bioRxiv - Immunology 2024Quote: ... were stained after cell fixation and permeabilization with True-NuclearTM transcription factor buffer set per protocol (BioLegend). Data were collected on BD LSR Fortessa or Beckman CytoFLEX flow cytometer and analyzed by FlowJo (Tree Star Inc. ...
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bioRxiv - Immunology 2024Quote: ... When appropriate NK cells were fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 57,58 Bone marrow cells were cultured with 20 ng/ml of macrophage colony-stimulating factor (BioLegend #576404) for 7 days as previously described 58 to generate BMDMs ...
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bioRxiv - Physiology 2023Quote: ... then seeded in 2mL of conditioned media with macrophage colony-stimulating factor (mCSF, 25 ng/mL, BioLegend). Five days later ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant galectin-3 (Biolegend # 599706) was then incubated either for 1-2 hrs at RT or overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... and GATA-3-AP488 (Biolegend) was carried out using the same method but without stimulation.
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bioRxiv - Immunology 2020Quote: ... 3) CD62L APC-Cy7 (BioLegend), huCD45 PE (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... LAG-3-BV711 (11C3C65, Biolegend) and TIGIT-BV605 (A15153G ...
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bioRxiv - Microbiology 2020Quote: ... and human Hashtag 3 (Biolegend) at 4°C for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... LAG-3 (BioLegend, 125212, C9B7W), ST2 (eBioscience ...