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Citations for BioLegend :
351 - 400 of 7136 citations for Thiol Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and plate-bound Armenian hamster anti-mouse CD3ε (clone 145-2C11, BioLegend, USA ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... cell culture plates were pre-coated with anti-mouse CD3 antibody (Biolegend) (1 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... in the presence of plate- bound anti-CD3 (5 μg/mL, Biolegend), CpG (10 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed and developed with 50 µl TMB (Biolegend, UK); after 5 minutes the reaction was stopped with 50 µl 1M sulfuric acid and absorbance measured at 450 and 630 nm ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated on a plate coated with anti-CD140a (#135902, Biolegend) for OPC selection ...
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bioRxiv - Immunology 2022Quote: ... plates were coated with brain lysates in an ELISA Coating Buffer (Biolegend) instead of mouse or human IgG antibodies.
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bioRxiv - Immunology 2024Quote: ... ELISPOT plates were coated with capture α-IFNγ (clone R4-6A2, Biolegend) and subsequently blocked ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were coated with 10 μg/ml anti-CD3 (145-2C11, Biolegend) for 2 h at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... For immune cells detections the tumoroids sections also were incubated overnight at 4°C with anti-B220 (Biolegend 103224, APC-cy7 conjugated) and anti-cd11b (Biolegend 101208 ...
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bioRxiv - Microbiology 2022Quote: IL-1 cytokine concentrations in supernatants from cultured BMDMΦ were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (31,50).
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bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Immunology 2021Quote: ... cells were restimulated with cognate antigens for three days and then supernatants were collected for IL-17A and IFNγ detection with ELISA (Biolegend, San Diego, CA). For determination of IL-17A+ and IFNγ+ CD45.1+ OT-II CD4+ T cell percentages ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed and incubated with goat IgG anti-mouse IgA-HRP (BioLegend). Plates were washed and developed in the dark using TMB substrate ...
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bioRxiv - Microbiology 2020Quote: ... The cell culture plates were pre-coated with anti-mouse CD3 antibody (Biolegend) (1 μg/ml) ...
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bioRxiv - Immunology 2020Quote: ... T cell activation was performed using plate-bound α-CD3 (clone OKT3, Biolegend) and α-CD28 (clone 9.3 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were restimulated on plates coated with anti-CD3 and anti-CD28 (BioLegend). After 48 hours of restimulation ...
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was provided by plate-bound αCD3 (clone HIT3a, BioLegend, cat#300313) at 1μg/mL unless otherwise specified ...
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bioRxiv - Immunology 2022Quote: ... plates were washed and incubated with 100 μL/well TMB-substrate (Biolegend/ITK). The substrate reacted approximately 8 minutes ...
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bioRxiv - Immunology 2022Quote: ... T cells were activated with plate-bound anti-CD3ε (clone 145-2C11; Biolegend) and 0.5 µg/ml of soluble anti-CD28 (clone 37.51 ...
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bioRxiv - Bioengineering 2024Quote: ... Plates were then washed with PBS before the addition of 1x streptavidin (Biolegend) in PBS + 0.5% BSA (w/v ...
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bioRxiv - Bioengineering 2024Quote: ... and then activated by pre-coated plate-bound anti-CD3 (1μg/ml; Biolegend) and soluble anti-CD28 ((1μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... ELISA assay plates were washed twice with 1x ELISA wash buffer (Biolegend, 421601), blocked for 1 hour at RT with 1x ELISA dilutant buffer (Biolegend Cat# 421203) ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/ml anti-mouse-IgM or IgG (Biolegend) in NaHCO3 0.1 M pH 9.6 ...
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bioRxiv - Systems Biology 2019Quote: ... and IL-6 secreted protein level in cell supernatant from 1×106 human MoDM’s was performed by Legend Plex multi-analyte assay kit (BioLegend) according to manufacturer’s instruction ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101; TNFα ELISA kit, BioLegend # 430204) or using LEGENDplex™ Human CD8/NK Panel Kit (Cat# 741186) ...
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bioRxiv - Bioengineering 2021Quote: ... IL6 was quantified using an ELISA developed from an antibody pair (biotin anti-rat IL6 detection antibody, cat. #517703, and purified anti-rat IL6 coating antibody, cat. #517701, Biolegend, San Diego, CA, USA). For this ELISA ...
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bioRxiv - Immunology 2021Quote: 96-well plates (Nunc, MaxiSorp, flat bottom, uncoated, BioLegend, San Diego, CA, USA, 423501) were coated with 0.5 μg/mL rhCTLA-4-Fc chimera (7268-CT ...
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bioRxiv - Cancer Biology 2022Quote: ... 96-well plates were coated with 5 g/ml of anti-CD28 antibody (Biolegend) o/n at 4°C to provide a co-stimulatory signal during co-culture ...
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bioRxiv - Immunology 2022Quote: ... Tissue and serum samples were loaded directly onto ELISA plates according to protocol (Biolegend 439407 Legend Max Mouse IFN-B ELISA kit) ...
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bioRxiv - Cancer Biology 2019Quote: ... and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend, CA ...
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bioRxiv - Immunology 2022Quote: ... After the plate was washed five times and a 3,3′,5,5′-Tetramethylbenzidine solution (Biolegend) was prepared and incubated for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333) with media containing soluble CD28 (Biolegend #302943 ...
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bioRxiv - Immunology 2020Quote: ... - plates were coated with Goat anti-mouse IgG (clone Poly4053 at 10μg/ml - BioLegend) for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2022Quote: ... in PBS were added to 96-well high-bound Nunc MaxiSorp ELISA plates (BioLegend) and incubated at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... the plate was further incubated with HRP-conjugated goat anti-mouse IgG (poly4053, Biolegend) at 1:20,000 for 1 h before development with TMB Substrate Set (421101 ...
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bioRxiv - Immunology 2023Quote: ... T cells were incubated in 96 well plates with Brefeldin A (15µg/mL, Biolegend) and stimulated with PMA (5ng/mL ...
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bioRxiv - Immunology 2024Quote: Plates were coated with 1.25 µg/ml anti-CD3 (145-2C11 or OKT3, Biolegend) with or without 10 µg/ml Fc proteins (R&D Systems Inc.) ...
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Immunology 2022Quote: ... LN and thymic homogenates were stained with a cocktail of antibodies against CD4-FITC and CD25-APC (for LN Tregulatory cells; Mouse Regulatory T Cell Staining Kit #1, eBioscience) or CD4-PB (Biolegend), CD8-FITC ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were either stained with DAPI for viability or fixed with 1% PFA and stained with zombie aqua fixable viability kit (Biolegend). Viable cells were sorted on a FACSAria flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2022Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Near-IR staining, dilution 1:500, Biolegend).