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Citations for BioLegend :
351 - 400 of 3749 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... in the presence of Fc Block (BioLegend) and fixable viability dye (Ghost Violet 510 ...
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bioRxiv - Cancer Biology 2022Quote: ... and Fc-Block (BD Biosciences or Biolegend) for 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... with anti-CD16/32 Fc-Block (BioLegend), then stained with the following antibodies for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse FC block 1:50 (BioLegend, 101320). Methoxy-X04 positive and negative microglia (CD11b+ ...
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bioRxiv - Cell Biology 2019Quote: ... Fc Block (BioLegend, 422302, dilution 1:20). Stained cells were analyzed using the LSRFortessa (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... and Fc Block (CD16/CD32, Tonbo, BioLegend) for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked (αCD16/32, BioLegend) followed by surface staining and analyzed by flow cytometry on an LSRFortessa or FACSymphony (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... Fc-blocked for 10 minutes (FcX, Biolegend) and stained with the surface antibody cocktail (reconstituted in CSM ...
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bioRxiv - Immunology 2022Quote: ... added 1uL of TruStain FC Blocker (Biolegend) per 1e6 cells ...
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bioRxiv - Genomics 2022Quote: ... Fc block (BioLegend, USA; 5μl per sample); wash buffer (600μl per sample) ...
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bioRxiv - Immunology 2022Quote: After Fc-blocking using FcX reagents (BioLegend), cells were incubated with primary antibodies in FACS buffer for 30 min at 4°C and if necessary ...
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bioRxiv - Immunology 2023Quote: ... blocked with Fc receptor (Biolegend, CA, USA), and stained with CD3-PE (clone HIT3a ...
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bioRxiv - Immunology 2023Quote: ... Samples were Fc-blocked (BioLegend, cat #422302) on ice for 5 minutes and stained with anti-β2m Platinum (Pt ...
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bioRxiv - Immunology 2023Quote: ... Fc-Block CD16/32 (Cat# 156604-BioLegend) was used to restrict non-specific antibody binding ...
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bioRxiv - Immunology 2023Quote: ... and Fc block (Biolegend, Cat. no. 422302) for 10 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in Fc-Block (BioLegend) containing PBS/2% FCS ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in Fc-Block (BioLegend) containing PBS/2% FCS buffer ...
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bioRxiv - Immunology 2024Quote: ... and then incubated with Fc block (Biolegend) in FACS buffer (PBS ...
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bioRxiv - Immunology 2024Quote: ... treated with Fc Block (Biolegend Trustain 101320), and resuspended in FACS buffer (HBSS-Gibco 10010-023 with 2% FBS Gibco ...
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bioRxiv - Microbiology 2024Quote: ... and blocked with Fc block (Biolegend, 101302) to prevent nonspecific staining ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with TruStain (Biolegend) and then stained with antibodies for 20 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... supernatant was collected at day 1 and 5 for LEGENDplexTM Human T Helper Cytokine Panel (BioLegend).
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bioRxiv - Neuroscience 2023Quote: ... Microglia were stained with 5 ul each of PE-anti human CD45 antibody clone HI30 (Biolegend) and APC-anti human CD11b antibody clone ICRF44 (Biolegend ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Immunology 2020Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
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bioRxiv - Cell Biology 2022Quote: ... 1µg/mL for FC) APC mouse IgG1, k isotype Control (400120, 1µg/mL for FC) were purchased from Biolegend.
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bioRxiv - Cancer Biology 2023Quote: ... Two million viable cells were resuspended in 100µl FACS buffer with 1 µl of Fc Block (anti-mouse Fc block CD16/32, Cat# 101302, Biolegend) for 20 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... immunohistochemistry (IHC) and flow-cytometry (FC): Pacific Blue anti-mouse Ly-6A/E (Biolegend, Cat#108120, 1:50 FC), Sca-1/Ly6A/E (E13 161-7 ...
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bioRxiv - Cell Biology 2021Quote: ... and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M; 1:1000, Biolegend, 637314) for 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Two microliters of mouse anti-hexa His antibody (BioLegend) were added to the 10 mL sample and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 100μl of HRP anti-His Tag Antibody (BioLegend, USA) (diluted 1:20000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and a HRP anti-His tag antibody (Biolegend, #652504) diluted 1:1000 in Superblock (Thermo #37515 ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated anti-His tag secondary antibody (BioLegend, 652503) diluted 1:2000 in blocking buffer was then added to the plates and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2022Quote: Mouse anti-His Tag antibody (clone J099B12, BioLegend #652502), goat anti-mouse IgG ...
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bioRxiv - Bioengineering 2024Quote: ... we used HRP anti-His tag antibody (BioLegend 652503).
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-FLAG-PE conjugate (BioLegend), rabbit (DA1E ...
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bioRxiv - Genetics 2022Quote: ... or 2μg FLAG antibody (BioLegend 637302). Beads and antibody were incubated overnight with rocking at 4°C.
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bioRxiv - Immunology 2020Quote: ... anti-FLAG tag (Clone L5, Biolegend), anti-GAPDH (clone 6C5 ...
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bioRxiv - Immunology 2020Quote: ... anti-FLAG tag (Clone L5, Biolegend) and anti-MICA (clone 159227 ...
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bioRxiv - Microbiology 2020Quote: ... rat anti-Flag mAb (L5, Biolegend), allophycocyanin (APC)-conjugated donkey anti-mouse IgG Fc fragment Ab ...
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bioRxiv - Immunology 2023Quote: ... supernatants from cell cultures were harvested for detecting cytokine using LEGENDplexTM Human Th1 Panel (5-Plex) (BioLegend). Samples were diluted 5-fold using assay buffer and then mixed with beads and shaken for 2 hours at 500 rpm in a 96 plate well ...
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... in complete medium supplemented with anti-human CD28 antibody (5 µg/mL) (BioLegend, San Diego, CA, US), rhIL2 (50 U/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... To prevent antibodies from binding to Fc-receptors we treated the cell suspension with Fc-block (Biolegend #101301; 1:50) prior using other antibodies ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in their residual buffer before being incubated with anti-CD16/CD32 (‘Fc Block’) to reduce non-specific binding to Fc receptors (Biolegend). After 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Juvenile islet cells from SIX3-Flag pseudoislets were stained with anti-FLAG antibody-555 (1:100; Biolegend). Labeled cells were sorted on a special order 5-laser FACS Aria II (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Cells were subsequently incubated with Fc Block (Biolegend) and fluorescent cell surface marker Abs and were then fixed and permeabilized using Fixation and Perm/Wash buffers (eBioscience) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with FC block (BioLegend, #422302) diluted in CSB (1:50 ...