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Citations for BioLegend :
51 - 100 of 1091 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... RBD was then detected using an Alexa Fluor 488 conjugated anti-His antibody (clone J099B1, Biolegend). Cells were analyzed on a flow cytometry (BD).
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bioRxiv - Immunology 2021Quote: ... PBMCs were stimulated with 2 μg/mL of indicated COVID-19 antigen peptide pools (RBD or Spike or NP) in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37 □ overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Confirmation of successful bead conjugation was determined by staining with anti-His Tag (Biolegend) and flow cytometry analysis ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The Western blot membranes were stained with HRP anti-His tag antibody (Biolegend, #652504) diluted 1:2,000 in 0.5% (w/v ...
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bioRxiv - Microbiology 2021Quote: Mouse anti-HA.11 (Clone 16B12) and Mouse anti-HIS tag (Clone J099B12) were purchased from BioLegend. Rat anti-HA (Clone 3F10 ...
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bioRxiv - Bioengineering 2023Quote: The antibodies used for flow cytometry were as follows: anti-His Tag APC (BioLegend, USA, Cat: #362605), anti-human IgG (G18-145 ...
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bioRxiv - Microbiology 2020Quote: ... incubated for 1 hour with mouse HRP α-His Tag antibody (1:1000 in 5% milk) (BioLegend, USA) and developed ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2022Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag) (SinoBiological, 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 or Alexa fluor 594 or FITC or Alexa fluor 648 labeled anti-His-tag (MBL or Biolegend),
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bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Immunology 2022Quote: ... CD300LG (Biolegend, see Table S1), and the Ulex europaeus agglutinin I (UEA1 ...
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bioRxiv - Immunology 2021Quote: ... WIV1 RBD-BV650 (streptavidin-BV650 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2021Quote: ... Wuhan RBD with PerCP-Cy5.5-streptavidin (BioLegend), Beta-RBD with BUV661-streptavidin (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... RBD tetramers were assembled by combining biotinylated RBD with streptavidin-Brilliant Violet 421 or streptavidin-Brilliant Violet 650 (both BioLegend) at a molar ratio of 4:1 ...
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bioRxiv - Immunology 2020Quote: ... RBD tetramers were assembled by combining biotinylated RBD with streptavidin-Brilliant Violet 421 or streptavidin-Brilliant Violet 650 (both BioLegend) at a molar ratio of 4:1 ...
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bioRxiv - Immunology 2021Quote: ... RBD was mixed with SA-PE (Biolegend, 405204) and SA-APC (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and 0.5µg of biotinylated RBD with BV421 (BioLegend) were incubated for 30 minutes in the dark to generate fluorochrome-linked biotinylated tetramers ...
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bioRxiv - Immunology 2023Quote: ... RBD protein conjugated with PE-streptavidin (BioLegend, 405204) and APC-streptavidin (BioLegend ...
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bioRxiv - Immunology 2023Quote: Biotinylated S1 protein was obtained commercially (BioLegend). A 4X molar excess was incubated with streptavidin-Alexa Fluor 647 (0.5 mg/mL ...
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bioRxiv - Microbiology 2022Quote: Corning ELISA plate wells were coated with 100µL of 100 μg/mL FCoV2 whole virus antigen or 100 μg/mL SCoV2 RBD or FCoV RBD antigen in sodium bicarbonate ELISA coating buffer at pH 9.5 (BioLegend, San Diego, CA) and incubated overnight at RT ...
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bioRxiv - Immunology 2023Quote: ... and RBD to streptavidin-APC (BioLegend, TotalSeq™-C0956) overnight at 4°C.
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bioRxiv - Microbiology 2020Quote: ... anti-His PE (Biolegend), and human Hashtag 3 (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... V5 tag (BioLegend, #903801), HA tag (Roche ...
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bioRxiv - Physiology 2019Quote: ... HA epitope tag (Biolegend clone HA.11 ...
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bioRxiv - Bioengineering 2021Quote: ... Biotinylated RBD was incubated with either streptavidin-conjugated PE (Biolegend) or streptavidin-conjugated APC fluorophores (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated RBD was mixed with streptavidin PECy7 (BioLegend, Cat# 405206) at 2.2:1 ratio (~4:1 molar ratio) ...
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bioRxiv - Bioengineering 2021Quote: ... Biotinylated RBD was incubated with either streptavidin-conjugated PE (Biolegend) or streptavidin-conjugated APC fluorophores (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... WT RBD were labled with TotalSeq-C0973 Streptavidin (Biolegend, 405275) and TotalSeq-C0974 Streptavidin (Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... the commercial RBD-specific monoclonal mouse IgG2a (BioLegend CAT#944803) as the known reference to construct standard curves for detection of RBD-specific IgG and IgG2a ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated Spike was conjugated to streptavidin-FITC (BioLegend) and streptavidin-PE (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and anti-His PE (Biolegend), at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... FLAG tag (monoclonal 637301, BioLegend) and GFP (polyclonal A-11122 ...
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bioRxiv - Cancer Biology 2021Quote: ... or Tag-it Violet (Biolegend) cell tracking dyes for 25 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... antibodies against HA tag (Biolegend), or V5 tag (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2021Quote: ... The His tagged ACE2 variants were detected using anti-His mAb (Cat. No. 652505, BioLegend).
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... WT biotinylated RBD protein conjugated with TotalSeq™-C0973 Streptavidin (BioLegend, 405275) and TotalSeq™-C0974 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... anti-IgM and RBD-647 and a TotalSeq-C hashtag oligo (Biolegend). RBD-647+ B cells were sorted on a FACS Aria (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... C-terminal HA-tags were detected using Direct-Blot™ HRP anti-DYKDDDDK Tag Antibody (BioLegend). The blots were developed with the SuperSignal West Femto Chemiluminescent system (Pierce ...
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bioRxiv - Genomics 2021Quote: ... the antibody solution of 52 TotalSeqC antibodies (BioLegend; Supplementary Table S1) was washed on a pre-wet Amicon Ultra-0.5 Centrifugal Filter to remove sodium azide ...
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bioRxiv - Immunology 2021Quote: ... anti-AU1 tag (clone AU1, BioLegend), anti-AU5 tag (clone AU5 ...