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Citations for BioLegend :
201 - 250 of 1054 citations for SARS CoV 2 Nucleoprotein His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: VLP-producing Expi293F cells were extracellularly and intracellularly stained with Allophycocyanin (APC) anti-Flag (DYKDDDDK) tag antibody (1:500) (Biolegend) and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24 ...
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bioRxiv - Immunology 2023Quote: ... The hCMV-peptide pool stimulated cells from each donor were washed and individually stained with Cell-hashtag (HTO:Hash Tag Oligo) TotalSeq C antibody (0.5 µg/condition; Biolegend, Table S2B) as per manufacturer’s recommendations for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (Biolegend), CD127 ...
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801 ...
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bioRxiv - Immunology 2021Quote: ... 0.1% BSA) before secondary detection with 1:100 dilutions of APC- conjugated mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Biophysics 2021Quote: ... The chambers were then incubated with 10 μg/ml purified Anti-HA 11 Epitope tag antibody (BioLegend, San Diego, CA, USA) in BIB at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue homogenates were centrifuged to remove tissue debris before being incubated with Anti-HA.11 Epitope Tag Antibody (Biolegend, Cat# 901515) for 4 hours in a microcentrifuge tube on a microtube rotator kept at 4°C ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-human CD47 antibody conjugated to APC (323123) and anti-HA Alexa Fluor® 488 anti-HA.11 Epitope Tag (901509) were purchased from Biolegend. The antibody to ORF3a was a gift from Prof ...
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bioRxiv - Immunology 2021Quote: Four different TotalSeq™-C antibodies were used for Hash Tag Oligonucleotide (HTO) labelling before cell sorting (Biolegend, M1/42M30-F11) following the manufacturer’s standard protocol ...
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bioRxiv - Cell Biology 2021Quote: Analysis of CAR expression on transduced NK92 cells was done by staining with APC-conjugated anti-human CD314 (NKG2D) mAbs and anti-Flag Tag mAbs (Biolegend, USA). NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were labeled with 5 µg ml−1 carboxylfluorescein diacetate succinimidyl ester (CFSE) or Tag-IT Violet™ proliferation and cell tracking dye (Biolegend) in PBS containing 5 mM MgSO4 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation for 10 minutes at 20,000 Xg at 4 °C and VASP was subjected to immunoprecipitation using either His6- specific epitope tag affinity matrix (Biolegend #900501) or control antibody matrix (Novagen #69026) ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blotting was performed using an anti-HA mouse monoclonal antibody (Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody, BioLegend) as primary antibody diluted 1:1000 in TBS-T (20 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... and cultured in presence of IL-2 (2 ng/ml; Biolegend), IL-7 (2.5 ng/ml ...
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bioRxiv - Immunology 2019Quote: ... CD138(BV421, 281-2, BioLegend, #142507; BV711, 281-2, BioLegend, #142519), FAS (PE ...
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bioRxiv - Immunology 2024Quote: ... and 10 ng/mL recombinant mouse interleukin-2 (IL-2) (BioLegend); additionally ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Biophysics 2020Quote: The antibodies used for these experiments were mouse purified anti-HA.11 Epitope Tag monoclonal (clone 16B12) (Biolegend 901502; 1:500 dilution), rabbit affinity-isolated anti-FLAG polyclonal (Sigma-Aldrich F7425 ...
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently washed twice with PBSF and labeled with 50 μL of APC-conjugated monoclonal mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, Cat # 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... IgD (IA6-2, BioLegend), CD27 (M-T271 ...
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bioRxiv - Immunology 2021Quote: ... TRAIL (Biolegend, RIK-2), FasL (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... 2 μM monensin (BioLegend) and 3 μM brefeldin A (BD ...
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bioRxiv - Immunology 2020Quote: ... IL-2 (Biolegend, #431004) and IL-22 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... CD32 (FUN-2; BioLegend), CD95 (DX2 ...
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bioRxiv - Immunology 2021Quote: ... CD138(281-2, BioLegend), CD38(90 ...
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bioRxiv - Immunology 2021Quote: ... IgD (IA6-2, BioLegend), CD27 (M-T271 ...
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bioRxiv - Immunology 2022Quote: ... integrinβ2 (M18/2; BioLegend), IgE (R35-72 ...
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bioRxiv - Synthetic Biology 2023Quote: ... IL-2 (Biolegend; 431804), CCL1 (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (Biolegend, #575404), IL-1β (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... or 2 (Biolegend #155863) for 20 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and IL-2 (BioLegend) were quantified by ELISA according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... CD8b.2 (Biolegend, 140416), Granzyme B (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (BioLegend #503824), IFNγ (Invitrogen #25731182) ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (BioLegend #503824), IFNγ (Invitrogen #25731182) ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Immunology 2021Quote: ... CAR expression analysis was performed using incubation with His-tagged recombinant CD123 protein (SinoBiological, Beijing, China) and secondary staining with αHis-PE or αHis-APC (BioLegend, San Diego, CA). Dead cells were excluded from analysis using LIVE/DEAD Fixable Viability Stains 780 or 575V (BD Horizon ...
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bioRxiv - Immunology 2022Quote: ... 2 μl PE/Cyanine7 anti-human IgD antibody (BioLegend, 348210, clone: IA6-2), 0.13 μg biotinylated SARS-CoV-2 BA.2 RBD protein (customized from Sino Biological ...
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bioRxiv - Physiology 2019Quote: ... cells were stained with Tag-It Violet™ Proliferation and Cell Tracking Dye according to the manufacturer’s instructions (Catalog #425101, Biolegend, San Diego, CA). Briefly ...
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bioRxiv - Immunology 2023Quote: For GBP2 co-adoptively transfer experiment MutuDC1-dCAS9KRAB sgNT versus MutuDC1-dCAS9KRAB sgGBP2 cells were stained with Tag-it Violet™ Proliferation and Cell Tracking Dye (Biolegend Cat.425101) or CellTrace™ Far Red Cell Proliferation Kit (Invitrogen Cat.C34564) ...
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bioRxiv - Immunology 2024Quote: ... and incubated at 37°C for 30 min with either a 1/500 dilution of PE-labeled anti-6xHis tag mouse MAb (Biolegend, San Diego, CA) to detect VHHs or Alexa Fluor 647-labeled goat anti-human IgG (H+L ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Biophysics 2021Quote: ... Synomag-D+ DC were intradermally injected into each hind footpad immediately following Tag-It Violet™ (Violet+) cell tracking dye incorporation (Biolegend, San Diego, USA). MPI was done immediately following injection (day 0 ...
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bioRxiv - Cell Biology 2020Quote: ... Diamidino-2-Phenylindole (DAPI) (Biolegend) for live/dead exclusion and cell sorting ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (MQ1-17H12, Biolegend), and IFNγ (4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... and 2 μM monensin (BioLegend). For intracellular staining of IgM ...
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bioRxiv - Cancer Biology 2022Quote: ... and monensin (2 μM, Biolegend) in a humidified incubator at 37°C with 5% CO2 ...