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Citations for BioLegend :
451 - 500 of 2367 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... ELISA assay to measure VEGFA levels was performed according to the manufacturers’ protocol (Biolegend, 430204) using the lung cancer cell supernatant.
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bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
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bioRxiv - Immunology 2022Quote: ... Antibody binding to CD16 and CD32 was prevented by incubation of macrophages with 0.5 μg Trustain FcX (Biolegend) in RB supplemented with 10% FCS for 10 min on ice ...
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bioRxiv - Immunology 2023Quote: ... and unspecific binding of antibodies to Fc receptors was blocked by incubating the cells with TruStain FcXTM (Biolegend) diluted 1:50 in FACS buffer for 15 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Binding of anti-TIGIT antibodies HB0036/HB0030 to cell surface was detected by APC-anti his (Biolegend/362605). For ELISA based assay of anti-TIGIT antibodies in tumour digest ...
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bioRxiv - Bioengineering 2023Quote: ... were prepared in 1× Annexin V Binding Buffer (100 mM HEPES, 25 mM CaCl2, 1.4 M NaCl; BioLegend). To analyze cells ...
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bioRxiv - Cell Biology 2021Quote: ... About 0.3 million cells in 37.5 μl CSM were loaded in each well of a 96-well plate of the Human Cell Surface Marker Screening (phycoerythrin [PE]) Kit (Biolegend). Each well contained 12.5 μl of diluted PE-conjugated antibody in CSM ...
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 and 5 μg/mL Brefeldin A (BFA; BioLegend) was added during the last 6 h of stimulation ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked by incubating cells with human anti-Fc Receptor antibodies (TrueStain, Biolegend, Cat. No. 101320) or anti-mouse CD16/32 Fc block (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific antibody binding was blocked by adding 10 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) to each sample and incubating for 10 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... washed once in PBS containing 0.9 mM Ca2+ and 0.5 mM Mg2+ and then stained with AnnV-FITC for 15 minutes in Annexin binding buffer at room temperature according to the manufacturer’s protocol (Biolegend). Cells were co-stained with DAPI at a final concentration of 1 µg/ml for 2 minutes in Annexin binding buffer ...
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bioRxiv - Immunology 2020Quote: Non-specific binding of antibodies to Fc-receptors was blocked with anti-mouse CD16/CD32 (TruStain fcX from BioLegend) in single cell suspensions prepared from Spl ...
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bioRxiv - Immunology 2020Quote: ... ACE2-HA binding was detected using anti-HA antibody HA.11 (clone 16B12, BioLegend, San Diego, CA, MMS-101P), HRP-conjugated anti-mouse IgG (Dianova ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with Annexin V-FITC (5uL in 0.14 mL Annexin V Binding Buffer, #640914; Biolegend, San Diego, CA) for 10 minutes at 4°C in darkness ...
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bioRxiv - Immunology 2023Quote: ... washed once in Annexin V buffer and resuspended in Annexin V binding buffer with Annexin V-BV421 (Biolegend; 640924) and incubated for 15 minutes at room temperature protected from the light ...
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bioRxiv - Immunology 2022Quote: ... IL-1α and IL-6 were quantified with Mouse IFN-γ ELISA MAXTM Deluxe Set (Biolegend), Mouse IL-1α ELISA MAXTM Deluxe Set (Biolegend ...
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bioRxiv - Synthetic Biology 2020Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFN◻ by ELISA (BioLegend). All assays were performed in triplicate and average IFN◻ production was plotted with standard deviation.
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bioRxiv - Synthetic Biology 2021Quote: ... and the cell lysates were assessed on a human IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cytokine secretion in the supernatant was evaluated by ELISA MAX Deluxe Set Human TNF-α (BioLegend), ELISA MAX Deluxe Set Human IFN-γ (BioLegend) ...
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Immunology 2024Quote: ... and IL-1β were carried out using ELISA MAXTM Standard Set (Biolegend, San Diego, CA, USA). In brief ...
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bioRxiv - Cell Biology 2021Quote: ... IL-5 (BioLegend) and IL-13 (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Single Cell suspensions were incubated with an anti-FcγIII/II receptor antibody (clone 93, eBioscience) to block unspecific binding and Zombie NIR™ (Biolegend), a fixable viability dye ...
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bioRxiv - Immunology 2022Quote: ... to block nonspecific binding and then were stained with dead dye to exclude dead cells (Aqua, ThermoFisher or Zombie NIR, Biolegend) for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... and the DAXL-88 (anti-AXL) binding assay used Alexa Fluor 647 AffiniPure anti-Fc tag Goat Anti-Human IgG (410711, BioLegend). Anti-Human CD4 antibody (BD ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged at 400g for 3 minutes at room temperature and resuspended 50μL in PE-Annexin V antibody diluted 1:20 with Annexin V Binding Buffer (BioLegend, 422201), or 50uL Annexin V Binding Buffer only for controls ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were incubated with cell surface markers for one hour at 4 °C and then washed with PBS before being resuspended with Annexin V binding buffer (Biolegend). Cells were then incubated with FITC Annexin V (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged S1-RBD was detected with Alexa Fluor 488 anti-his tag antibody (clone J095G46, Biolegend, UK) 1:5000 in 25 μL PBST + 0.1% BSA per well ...
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bioRxiv - Cancer Biology 2020Quote: After 7 days of shRNA-mediated knockdown 1 x 105 cells were wash once in PBS and resuspend in Annexin-V Binding Buffer (422201, BioLegend) with 3 μl of Annexin-V-APC (640941 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... to block nonspecific binding and then cells were stained using a master mix of CD45.1 (Biolegend Cat # 110728, 1:1000), F4/80 (Biolegend Cat # 123110 ...
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bioRxiv - Immunology 2020Quote: ... Cells were surface stained and washed with FACS buffer then resuspended in 100 μL Annexin V binding buffer (Biolegend: 79998). Cells were then incubated with 5 μL APC Annexin V (Biolegend ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... To prevent antibodies from binding to Fc-receptors we treated the cell suspension with Fc-block (Biolegend #101301; 1:50) prior using other antibodies ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in their residual buffer before being incubated with anti-CD16/CD32 (‘Fc Block’) to reduce non-specific binding to Fc receptors (Biolegend). After 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... and NMHC-IIB antibody (#909901) binding to residues 1965-1976 in the C terminus (1:100 IF, 1:400 WB) were purchased from BioLegend. Rabbit polyclonal antibody against GFP tag (#50430-2-AP ...
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bioRxiv - Immunology 2024Quote: ... Efficiency of CD4+ T cell depletion was routinely assessed in small amounts of peripheral blood on d0 prior to LCMV challenge by flow cytometry staining with a non-competitively binding anti-CD4 antibody (clone RM4-4, Biolegend).
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bioRxiv - Biochemistry 2020Quote: Conditioned media samples were analyzed using commercially available ELISAs as per manufacturer guidelines (BioLegend, San Diego, CA), at 2X and 8X dilutions for IL-6 ...
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bioRxiv - Immunology 2020Quote: ... and homogenized lung were measured by ELISA according to the manufacturer’s instructions (Biolegend or R&D Systems). The levels of PGE2 were measured at the serum or the peritoneal lavage fluid of septic mice ...
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bioRxiv - Immunology 2022Quote: ... Collected supernatants were assessed for cytokine content using ELISA MAX™ Standard Set Mouse IFN-γ (Biolegend). DCs were analyzed for the expression of CD86 and MHC class II using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... with supernatant IFNγ level measured by enzyme-linked immunosorbent assay (ELISA) following manufacturer’s protocol (Biolegend, Cat# 430104).
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bioRxiv - Immunology 2023Quote: ... The level of produced both pro-inflammatory cytokines and anti-inflammatory cytokines were measured using elisa (Biolegend).
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Immunology 2019Quote: ... CD4 (RM4-5, Biolegend), CD4 (GK1.5 ...
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bioRxiv - Immunology 2022Quote: ... CD4 (RM4-5, BioLegend), CD3e (145-2C11 ...
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bioRxiv - Immunology 2020Quote: ... CD4 (RM4-5; BioLegend), CD8 (53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... CD4 (RM4-5, Biolegend), CD8a (53-6.7 ...