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Citations for BioLegend :
251 - 300 of 4689 citations for Recombinant Mouse Pdgfc Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... After adding Fc receptor blocker (BioLegend), cells were labeled overnight at 4°C with a cocktail of antibodies recognizing chromatin modifications or intracellular components ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block (BioLegend), followed by tetramer incubation (25μg/ml ...
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bioRxiv - Immunology 2022Quote: ... 5µg/ml CD80-Fc (555404, Biolegend), and 2µg/ml PD-L1-Fc (758206 ...
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bioRxiv - Immunology 2022Quote: ... stained with Fc receptor block (BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... After Fc blocking (TruStain FcX; BioLegend), cells were stained with a metal-conjugated surface antibody cocktail (Table 2) ...
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bioRxiv - Cancer Biology 2023Quote: ... performed with Fc blocking (422302, BioLegend), and labelled with anti-CD138 (142508 ...
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bioRxiv - Immunology 2024Quote: ... and incubated with Fc Block (Biolegend) for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: Bone marrow cells from femurs and tibias were cultured in presence of 20 ng/ml mouse recombinant GM-CSF (Biolegend) for 8 days ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were incubated for 48h with OVAp (10 ng/ml) and mouse recombinant IL2 (50 U/ml) (Cat# 575402, Biolegend), was added for a period of five additional days ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with an Alexa Fluor 647-labeled mouse monoclonal antibody against the human IgG1-Fc (Biolegend, San Diego, USA, cat #409320) and analyzed in a Gallios flow cytometer (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellets washed twice with 10% FCS in PBS (100μl/well) and then resuspended in 10% FCS in PBS (100μl/well) containing APC/Cyanine7 Goat anti-mouse IgG (Biolegend; 1:250) and incubated on ice for 30mins ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Immunology 2020Quote: Fc block was performed using TruStain FcX™ CD16/32 antibody (anti-mouse) to avoid non-specific binding (101320 Biolegend, UK) according to the supplier protocols The fluorochrome-conjugated antibodies used in the panel were obtained from Biolegend ...
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bioRxiv - Immunology 2022Quote: The following protocol was used for all tumor experiments: after the Fc receptor of the cells was blocked with anti-mouse CD16/32 (Biolegend, 101320), extracellular staining was performed in FACS buffer for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12p70 and recombinant human IL-18 at 10ng/ml (Biolegend; San Diego, CA).
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Cancer Biology 2019Quote: ... The cells were washed with HBSS twice by centrifugation at 250 x g for 8 min and then suspended in BMDC culture medium (RPMI-1640 medium [Thermo Fisher Scientific] containing 10% FBS, 20 ng/ml recombinant mouse GM-CSF [BioLegend] ...
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bioRxiv - Neuroscience 2020Quote: ... and IFNγ (recombinant rat IFNγ, Biolegend) at 50 ng/ml ...
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bioRxiv - Bioengineering 2020Quote: ... recombinant human IL-15 (570308, Biolegend), recombinant mouse scIL-12 (130-096 ...
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bioRxiv - Immunology 2022Quote: ... and recombinant 100ng/ml FLT3L (Biolegend). Cells were harvested by flushing petri dishes with cold PBS ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant hTGF-β2 (#583301, Biolegend, USA), recombinant hTGF-β3 (#501123524 ...
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bioRxiv - Cancer Biology 2019Quote: ... KSF-mIL12 and recombinant mIL12 (BioLegend) were subjected to a spleno-cyte IFN-γ release assay ...
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bioRxiv - Bioengineering 2020Quote: ... recombinant murine MMP-3 (#552704, BioLegend), recombinant murine c-RP (#50409-M08H ...
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bioRxiv - Immunology 2022Quote: ... and recombinant human TPO (763706; Biolegend) for HSC culture conditions and 10 ng/mL of SCF ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant human IL-15 (570308, Biolegend), recombinant mouse scIL-12 (130-096 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human interferon gamma (IFNγ; Biolegend) and recombinant human transforming growth factor beta-1 (TGF-β1 ...
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bioRxiv - Immunology 2023Quote: ... Recombinant cytokines were purchased from Biolegend.
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bioRxiv - Immunology 2023Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were then blocked by incubating cells in TruStain Fcx plus Fc block (1:100; BioLegend, 156604) in FACS buffer (DPBS containing 2% FBS and 2mM EDTA ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with human Fc receptor block (BioLegend), and stained with cell surface antibodies (1:100 anti-CD137-APC [clone 4B4-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... before Fc blockade with TruStain FcX (BioLegend) and subsequent surface marker staining ...
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bioRxiv - Cell Biology 2020Quote: ... Isotype Ctrl (clone Poly29108, Biolegend 910801, FC). For Odyssey detection of western blots ...
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bioRxiv - Immunology 2020Quote: ... Fc receptor blocking antibody (CD16/CD32; Biolegend), anti-EpCAM (clone G8.8 ...
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bioRxiv - Immunology 2022Quote: ... Fc receptor-blocking antibody (CD16/CD32; BioLegend), anti-EpCAM (clone G8.8 ...
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bioRxiv - Cancer Biology 2021Quote: ... or mPD-L1-FC (Biolegend, Cat#758208) and incubated at 4°C overnight before seeding cells.
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bioRxiv - Microbiology 2021Quote: ... in the presence of Fc Block (BioLegend) and fixable viability dye (Ghost Violet 510 ...