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Citations for BioLegend :
401 - 450 of 4473 citations for Recombinant Mouse Pcsk9 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... we coated the cells with 3.5 μg/ml biotinylated anti-CD81 (BioLegend, clone TAPA-1) antibody at the 37 °C for 30 min ...
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bioRxiv - Immunology 2023Quote: MHC-I tetramers were generated using the biotinylated Flex-T™ monomer UVX platform (Biolegend) for HLA-A*02:01 and HLA-A*03:01 according to manufacturer’s protocol with the modifications detailed below ...
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bioRxiv - Immunology 2023Quote: ... and stained for 5 min at 4°C with biotinylated anti-CD3 antibody (17A2; BioLegend) to exclude CD3-positive T cells in the cell suspension using anti-biotin microbeads (Miltenyi Biotec ...
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bioRxiv - Synthetic Biology 2021Quote: ... Anti-bodies used in this study included a monoclonal α-his-antibody (BioLegend, 652502) and polyclonal α-CsgA-antibody and α-TasA-antibody.
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bioRxiv - Bioengineering 2022Quote: ... Confirmation of successful bead conjugation was determined by staining with anti-His Tag (Biolegend) and flow cytometry analysis ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The Western blot membranes were stained with HRP anti-His tag antibody (Biolegend, #652504) diluted 1:2,000 in 0.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M; 1:1000, Biolegend, 637314) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... All enriched samples were stained with the following panel of TotalSeq-A-labeled anti-mouse antibodies to detect protein expression in the scRNA-seq dataset: Ly6C (HK1.4, BioLegend), CD44 (IM7 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunofluorescence on these tissues include: mouse anti-myelin basic protein (MBP) (1:1000; #808401 BioLegend), rabbit anti-MBP (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... a final concentration of 5ng/ml of recombinant human TGFβ1 (BioLegend, #580702) was added to the culture media and treated for 24~48 hours ...
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bioRxiv - Immunology 2022Quote: ... 100ng/ml of recombinant human (rh) IL-8 (Biolegend, San Diego, CA) were added to the apical side of the enteroid monolayers (76) ...
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bioRxiv - Immunology 2019Quote: ... 10% FBS and 20 ng/ml of murine recombinant M-CSF (Biolegend) at 37°C in a humidified incubator for 7–8 days ...
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bioRxiv - Microbiology 2022Quote: ... in the presence of 50 ng/mL recombinant human M-CSF (Biolegend) for 7 days ...
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bioRxiv - Microbiology 2022Quote: ... plated in complete RPMI with 50 ng/mL recombinant M-CSF (Biolegend) and cultured for 6 days with medium change every 3 days.
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bioRxiv - Cancer Biology 2020Quote: ... in DMEM/F12 supplemented with human recombinant EGF (20 ng/mL, BioLegend), bFGF (20 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR-NK cells were loaded with human recombinant PD-L1- Fc (Biolegend) or ErBb2-Fc fusion proteins (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... and 200 ng/mL recombinant human B cell-activating factor (BAFF, BioLegend).
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/mL human recombinant IL-3 (BioLegend, San Diego, CA, USA) and antibiotics ...
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bioRxiv - Microbiology 2023Quote: ... plated in complete RPMI with 50 ng/mL recombinant M-CSF (Biolegend) and cultured for 6 days with medium change every 3 days.
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bioRxiv - Microbiology 2023Quote: ... in the presence of 50 ng/mL recombinant human M-CSF (Biolegend) for 7 days ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human NRG1 was acquired from BioLegend (San Diego, CA, Cat. 551904). High-throughput drug screening assays were used with compounds sourced as indicated in Suppl ...
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bioRxiv - Cancer Biology 2024Quote: ... in the presence of 10 ng/mL recombinant MIF (Biolegend, cat. 599404) or MDK (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... CD4+CD25- T cells (Tconv) were purified by adding a biotinylated anti-CD25 antibody (PC61; Biolegend) to the CD4+ T cell negative isolation kit to deplete Treg cells ...
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bioRxiv - Cancer Biology 2021Quote: ... then cells were stained with antibodies for 30 mins on ice: biotinylated anti-PDPN (BioLegend 127404), biotinylated anti-PDGFRα (anti-CD140a ...
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bioRxiv - Cancer Biology 2022Quote: ... we incubated the beads with 3.5 μg/ml biotinylated anti-CD81 antibody (BioLegend, clone TAPA-1) at the room temperature for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with biotinylated detection antibodies against IL-12 (Biolegend Cat#505302, clone C17.8) and IL-1β (Biolegend Cat#515801 ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were stained with a biotinylated anti-human CD271 (NGFR) antibody (BioLegend, Cat# 345122). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... followed by the staining with anti-His-APC (1:20, clone J095G46, BioLegend cat# 362605) with Fc block (1:20 ...
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bioRxiv - Microbiology 2020Quote: ... Primary detection of TRX-tagged YpTop1 was with primary murine monoclonal anti-TRX (BioLegend #658902, 1μg/ml), ...
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bioRxiv - Synthetic Biology 2020Quote: HA-tagged Gal4 TAs were stained with anti-HA.11 directly conjugated to Alexa Fluor 594 (BioLegend catalogue #901511 ...
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Protein levels in plasma and skin tissue protein extracts were measured using defined analyte panels: Legendplex Mouse Inflammation Panel and Inflammatory Chemokine Panel (Biolegend).
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2020Quote: ... and were cultured with 50 ng/ml recombinant human GM-CSF (BioLegend #572904) and 50 ng/ml recombinant human M-CSF (BioLegend #574806 ...
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bioRxiv - Bioengineering 2021Quote: ... and 20 ng/mL recombinant human IL-3 (BioLegend; San Diego, CA, USA) to determine cellular distribution ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then treated with 100 ng/ml of recombinant human IFNγ (Biolegend) for 24h ...
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bioRxiv - Immunology 2021Quote: Mice were administered 0.75 µg of recombinant IL-33 (BioLegend, San Diego, CA) or PBS via intraperitoneal injection daily beginning at 3 days before amebic challenge up until 1 day before harvest.
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bioRxiv - Immunology 2022Quote: ... and 20ng/mL recombinant murine M-CSF (Shenandoah 200-08 or BioLegend 576404) in non-TC treated Petri dishes ...
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bioRxiv - Cancer Biology 2022Quote: ... “Rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). Scratches were imaged at 0 hours ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human SARS-CoV-2 spike S1 IgG lyophilized antibody (Clone AM009105, BioLegend) was used as a standard.
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 8µg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
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bioRxiv - Physiology 2019Quote: ... and stained with biotinylated antibodies against CD11b to identify myeloid cells (1:1000, catalog number 101204, BioLegend), CD3 to identify T cells (1:250 ...
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bioRxiv - Immunology 2021Quote: ... lung cells were surface stained with a biotinylated Y-Ae antibody followed by APC-Streptavidin (405207, Biolegend). Mouse H2-O expression was detected by staining intracellularly with an anti-mouse H2-Oβ antibody97 provided by L ...
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bioRxiv - Immunology 2022Quote: ... 0,5*106 CD4+ T cells were incubated with biotinylated 1μg/mL anti-CD3ε (Clone 145-2C11, BioLegend) alone or in combination with 2ug/mL biotinylated anti-CD28 (Clone 37,15 ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 5 min at 4°C with biotinylated anti-CD19 antibody (1D3; BioLegend) to exclude CD19-positive B cells in the cell suspension using anti-biotin microbeads and LS columns ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 8μg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
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bioRxiv - Immunology 2023Quote: CD4+ or CD8+ T cells were negatively sorted using biotinylated anti-B220 (clone RA3-6B2, Biolegend #103204), CD11b (clone M1/70 ...
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bioRxiv - Immunology 2021Quote: ... RBD was then detected using an Alexa Fluor 488 conjugated anti-His antibody (clone J099B1, Biolegend). Cells were analyzed on a flow cytometry (BD).
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bioRxiv - Biochemistry 2022Quote: ... one slide was probed with Alexa Fluor® 647 anti-his antibody (1:250, BioLegend, 362611). Sera/plasma samples were single or serial diluted (three-fold dilutions from 1:90 ...