Recombinant Mouse Cd83 His tagged citations for BioLegend

Quote: ... containing mouse TruStain FcX (BioLegend) and the mouse cell surface protein antibody panel containing 174 oligo-conjugated antibodies (Supplementary Table 1) ...

Tumor-resident regulatory T cells in pancreatic cancer express the αvβ5 integrin as a targetable activation marker

Kodai Suzuki, et al., bioRxiv - Cancer Biology 2023

Quote: ... IV PBS + IP anti-mouse PD-L1 mAb (200 μg/mouse; Ultra-LEAF™ purified anti-mouse PD-L1 Ab, clone 10F.9G2; BioLegend, San Diego, CA), IV iRGD + IP anti-mouse PD-L1 mAb ...

Single-cell transcriptomics reveal hyperacute cytokine and immune checkpoint axis in patients with poor neurological outcomes after cardiac arrest

Tomoyoshi Tamura, et al., bioRxiv - Immunology 2022

Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...

Modulation of immunosuppressant drug treatment to improve SARS-CoV-2 vaccine efficacy in mice

Amy V. Paschall, et al., bioRxiv - Immunology 2021

Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).

Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

Quote: ... neuronal activity was recorded for 15– 30 min daily before and after treatment with recombinant CXCL11 (200 nM, BioLegend #573606) using the Maestro Pro MEA System (Axion BioSystems) ...

Nuclear proteins acquiring Germinal Center B cells are specifically suppressed by follicular regulatory T cells

Fang Ke, et al., bioRxiv - Immunology 2022

Quote: ... 5×104 B cells were first incubated with 5 µg/ml anti-IgM complexes and 1 µg/ml recombinant Human CD40L (Biolegend) for 30 mins and then 1×104 Tfr cells were added ...

In vitro and in vivo effects of zoledronate on senescence and senescence-associated secretory phenotype markers

Parinya Samakkarnthai, et al., bioRxiv - Physiology 2023

Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...

Killing them softly with EPO: a new role for erythropoietin in the homeostasis of red blood cells

Clemente F. Arias, et al., bioRxiv - Physiology 2023

Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...

CD163 and Tim-4 identify resident intestinal macrophages across sub-tissular regions that are spatially regulated by TGF-β

Ian E. Prise, et al., bioRxiv - Immunology 2023

Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...

The lipid Gb3 promotes germinal center B cell responses and anti-viral immunity

Pankaj Sharma, et al., bioRxiv - Immunology 2023

Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...

Tissue damage during acute Trypanosoma cruzi infection is associated with reduced reparative regulatory T cell response and can be attenuated by early interleukin-33 administration

Santiago Boccardo, et al., bioRxiv - Immunology 2024

Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...

STING1 activation in brain endothelium by Plasmodium-sequestered heme leads to lethal cerebral malaria through activation of type I IFN response

Teresa Faria Pais, et al., bioRxiv - Immunology 2022

Quote: ... before staining with anti-mouse Granzyme B-efluor 450 and anti-mouse IFNγ-PE (BioLegend). Brain cells isolated from Pba-infected mice injected with BrdU were stained using the FITC BD BrdU Flow Kit (BD Pharmingen™ ...

NOD2 and TLR2 Signal via TBK1 and PI31 to Direct Cross-presentation and CD8 T Cell Responses

Daniele Corridoni, et al., bioRxiv - Immunology 2019

Quote: ... anti-mouse CD86 (GL1) and anti-mouse CD40 (Clone 3/23) were all from Biolegend.

Dose-dependent role of Gfi1 in murine hematopoietic stem cell self-renewal and differentiation

Judith Schütte, et al., bioRxiv - Molecular Biology 2019

Quote: ... FITC anti-mouse CD4 (100509) and FITC anti-mouse/human CD45R/B220 (103205) from BioLegend, Streptavidin PerCP-Cyanine5.5 (45-4317-82 ...

Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes

Alex S Genshaft, et al., bioRxiv - Systems Biology 2020

Quote: ... while mouse spleen samples were incubated with PE anti-mouse TCR β chain antibody (BioLegend) according to manufacturer’s instructions ...

Preclinical establishment of a divalent vaccine against SARS-CoV-2

Zsofia Hevesi, et al., bioRxiv - Bioengineering 2022

Quote: ... anti-mouse CD8 AlexFluor700 (cloneYTS156.7.7) and anti-mouse CD44 PerCP-Cy5.5 (clone IM7; all Biolegend) in PBS + 0.5% BSA + 0.05%NaN3 ...

Targeting advanced prostate cancer with STEAP1 chimeric antigen receptor T cell therapy

Vipul Bhatia, et al., bioRxiv - Cancer Biology 2022

Quote: ... Antibodies used for mouse cells are rat anti-mouse CD8a conjugated to FITC (BioLegend, 100706) and rat anti-mouse CD19 conjugated to PE (BioLegend ...

Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils

Valeria Manriquez, et al., bioRxiv - Microbiology 2021

Quote: ... 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit ...

A collaboration between immune cells and the choroid plexus epithelium in brain inflammation

Huixin Xu, et al., bioRxiv - Neuroscience 2023

Quote: ... Alexa594 anti-mouse Ly-6G/Ly-6C (Gr-1) antibody (BioLegend 108448, 10ug per mouse), rat Ultra-LEAF™ purified anti-mouse CD106 antibody ...

A collaboration between immune cells and the choroid plexus epithelium in brain inflammation

Huixin Xu, et al., bioRxiv - Neuroscience 2023

Quote: ... Alexa594 anti-mouse Ly-6G/Ly-6C (Gr-1) antibody (BioLegend 108448, 10ug per mouse) and PE rat anti-mouse CD62E (for E-selectin ...

PD-1 Mediated Regulation of Unique Activated CD8⁺ T Cells by NK Cells in the Submandibular Gland

Samantha M. Borys, et al., bioRxiv - Immunology 2023

Quote: ... and different TotalSeq-C Anti-Mouse Hashtags (TotalSeq-C Anti-Mouse Hashtags 1-10 Biolegend #155861-155879 (Supplemental Tables 1 and 2) ...

Brain capillary obstruction as a novel mechanism of anti-CD19 CAR T cell neurotoxicity

Lila D Faulhaber, et al., bioRxiv - Neuroscience 2021

Quote: ... or monoclonal fluorescent conjugated antibodies (rat anti-mouse CD3, clone 17A2; mouse anti-mouse CD45.2, clone 104; all from Biolegend and used at 0.4mg/kg i.v.).

Toll-like receptor 2 orchestrates a potent anti-tumor response in non-small cell lung cancer

Fraser R. Millar, et al., bioRxiv - Cancer Biology 2021

Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...

Phagocytic “teeth” and myosin-II “jaw” power target constriction during phagocytosis

Daan Vorselen, et al., bioRxiv - Cell Biology 2021

Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...

Engineering Defensin α-helix to produce high-affinity SARS-CoV-2 Spike protein binding ligands

Leonardo Antônio Fernandes, et al., bioRxiv - Bioengineering 2022

Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...

The N-terminal domain of spike glycoprotein mediates SARS-CoV-2 infection by associating with L-SIGN and DC-SIGN

Wai Tuck Soh, et al., bioRxiv - Microbiology 2020

Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...

A multi-enhancer hub at the Ets1 locus controls T cell differentiation and allergic inflammation through 3D genome topology

Aditi Chandra, et al., bioRxiv - Genomics 2022

Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...

ZEB1-dependent modulation of fibroblast polarization governs inflammation and immune checkpoint blockade sensitivity in colorectal cancer

Harald Schuhwerk, et al., bioRxiv - Cancer Biology 2023

Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...

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