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Citations for BioLegend :
151 - 200 of 3043 citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Bioengineering 2023Quote: ... APC-conjugated His-tag antibody (Biolegend) was added to the cells treated with no nanobody proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
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bioRxiv - Immunology 2021Quote: Recombinant mouse GCSF (BioLegend) was injected intraperitoneally (125μg/mouse ...
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bioRxiv - Immunology 2019Quote: ... recombinant murine SCF (BioLegend), recombinant murine G-CSF (BioLegend) ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant mouse IFNβ (BioLegend), PALA (Open Chemical Repository of the Developmental Therapeutics Program NCI/NIH) ...
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bioRxiv - Immunology 2021Quote: ... biotinylated anti-lineage cocktail (Biolegend, cat # 133307) followed by streptavidin-FITC (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated secondary antibodies were ordered from Biolegend.
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bioRxiv - Cancer Biology 2021Quote: ... biotinylated anti-PDGFRα (anti-CD140a, BioLegend 135910), PerCP/Cy5.5-conjugated anti-CD31 (BioLegend 102522) ...
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bioRxiv - Immunology 2023Quote: ... with biotinylated annexin V (Biolegend, Cat # 640904) and incubated for 30 minutes then centrifuged at 500 x g for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... or by consecutive cell sorting for the expression of fluorescent proteins (mCherry-tagged or mVenus-tagged constructs) or the re-expression of TLR2 at the cell surface (Clone T2.5, Biolegend 121810) until the cell population was homogeneous ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and tetramerized using fluorescently tagged streptavidin from Biolegend. 5,6-Dimethylxanthenone-4-acetic Acid (DMXAA ...
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bioRxiv - Immunology 2021Quote: ... metal-tagged antibodies including pan-keratin (C11, Biolegend), BDCA2 (Polyclonal ...
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bioRxiv - Immunology 2022Quote: The following human cytokines were assessed by ELISA as previously described (10): human IFN-γ (capture MD-1 mAb; revealing biotinylated 4S.B3 mAb, Biolegend Cat#507502 and 502504, respectively), human IL-13 (capture JES10-5A2 mAb ...
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bioRxiv - Immunology 2022Quote: ... and APC anti-His Tag antibody (Biolegend) for 30 min each ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant galectin-3 (Biolegend # 599706) was then incubated either for 1-2 hrs at RT or overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... recombinant murine G-CSF (BioLegend), recombinant murine ICAM-1 (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... Reagents: recombinant murine CXCL1 (BioLegend), recombinant murine SCF (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant murine IL-4 (Biolegend) was combined with rat IgG1 mAb to IL-4 ...
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bioRxiv - Immunology 2020Quote: Recombinant murine IL-33 (Biolegend) was injected intraperitoneally to C57BL/6 mice (100 ng/mouse) ...
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bioRxiv - Immunology 2021Quote: ... Carrier-free recombinant mouse (Biolegend) or human (SinoBiological ...
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bioRxiv - Immunology 2022Quote: ... recombinant mouse IL2 (Biolegend #575408) and recombinant murine GMCSF (Peprotech #315-03 ...
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bioRxiv - Immunology 2023Quote: ... and recombinant IL-17A (BioLegend). Streptavidin HRP (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... recombinant murine IFN-γ (Biolegend) or PBS was administered by IP injection at 0.25 mg/kg on days 3-8 of DSS exposure ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant BMP6 (rBMP6) (Biolegend, USA), recombinant Follistatin (rFST ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant Follistatin (rFST) (Biolegend, USA) or vehicle control were added to the desired concentration whilst still preconfluent (approximately 24 hours before confluency) ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Biochemistry 2022Quote: Moles of product formed in hydrolysis reactions used for ACE2 activity and specificity profiling were determined by comparing area under the curve for LC chromatograms corresponding to these reactions to areas under the curve observed for control reactions in which > 95% input substrate conversion was achieved by incubation of substrate with 25 nM recombinant human ACE2 commercial standard (BioLegend, San Diego, CA) for between one and four hours pending peptide substrate ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with M-CSF (100ng/ml) Recombinant Human M-CSF (Macrophage-Colony Stimulating Factor, BioLegend cat. 574806, San Diego, CA, USA) for 5 min ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated antibodies were stained with Strepatvidin-BV421 (Biolegend). Staining with propidium iodide (PI ...
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bioRxiv - Immunology 2020Quote: ... or biotinylated anti-mouse cKit (clone 2B8, BioLegend) with sAV-SAP in a 1:1 molar ratio for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... Then a biotinylated anti-IL-2 Ab (BioLegend) at a concentration of 0.25 μg/ml was added and after washing ...
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bioRxiv - Immunology 2021Quote: ... and biotinylated anti-CD45 antibody (BioLegend, 1:100). Immunohistochemistry using monoclonal mouse anti-β-catenin (BD Transduction Laboratories ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated anti-IFNγ R46A2 (0.5 μg/mL, Biolegend) was prepared in blocking solution and added to the plate ...
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bioRxiv - Neuroscience 2023Quote: ... biotinylated anti-HA (mouse, 1:500, BioLegend 901505), anti-CD68 (rat ...
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bioRxiv - Immunology 2022Quote: ... and 0.5µg of biotinylated RBD with BV421 (BioLegend) were incubated for 30 minutes in the dark to generate fluorochrome-linked biotinylated tetramers ...
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bioRxiv - Immunology 2022Quote: ... biotinylated mouse anti-rat IgG1 (Biolegend, Cat. 407404) or biotinylated mouse anti-rat IgG2a (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... rat biotinylated anti-CD45 (Biolegend, 5530, 1:200), rabbit anti-RXRα (D6H10 ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated lectins were detected using PE-Streptavidin (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Biotinylated Spike was conjugated to streptavidin-FITC (BioLegend) and streptavidin-PE (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Cell Biology 2021Quote: ... Fluorescently-tagged anti-mouse antibodies (BioLegend, San Diego, CA) were used to label cell surface markers for flow cytometry analysis ...
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bioRxiv - Immunology 2023Quote: ... and 7.5 nM of either a) tail-biotinylated cognate pMHC complex or b) biotinylated Armenian hamster anti-CD3ε antibody (clone 145-2C11, Biolegend 100304). ICAM-1 and pMHC proteins were expressed and purified as described (Wang et al. ...
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bioRxiv - Immunology 2021Quote: ... Samples were thawed and proteins quantified using a LEGENDplex Human Proinflammatory Chemokine Panel immunoassay (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2020Quote: ... Organoids were cultivated in growth medium as previously described (61) with minor changes (50% mouse Wnt3a-conditioned medium, 50ng/mL mouse Wnt3a recombinant protein (Biolegend, USA), 15% mouse R-spondin1-conditioned medium ...
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bioRxiv - Immunology 2020Quote: ... APC-anti-his tag antibody (Biolegend, clone: J095G46)) ...