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Citations for BioLegend :
101 - 150 of 2895 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Immunology 2023Quote: ... Soluble His6-tagged M6G at varying concentrations was incubated for 10 min with HRP-conjugated mouse anti-His antibody (BioLegend) diluted 1:2000 in 0.1% BSA/TBST ...
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bioRxiv - Genomics 2020Quote: ... Fc receptors were blocked by incubating the cells with 50 μl Human TruStain FcX (Biolegend, #422302) for 10 minutes at room temperature prior to antibody staining and sorting ...
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bioRxiv - Immunology 2022Quote: ... Whole blood cell staining was performed using an Fc receptor blocking solution (Human TruStain FcX; BioLegend), the viability dye eFluor780 (Thermo Fisher) ...
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bioRxiv - Immunology 2019Quote: ... suspended in PBS containing 1% BSA and an Fc receptor-blocking (human TruStainFcX; Biolegend, CA, USA). The neutrophils were then permeabilized with 0.1% saponin and incubated with a mAb against TLR9 conjugated with FITC (catalog number ab134369/clone 26C593.2 ...
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bioRxiv - Immunology 2023Quote: Zombie Aqua Fixable Viability Dye and Human TruStain FcX Fc Receptor Blocking Solution were from BioLegend. CF700-SE and CF750-SE were bought from Biotium ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... a final concentration of 5ng/ml of recombinant human TGFβ1 (BioLegend, #580702) was added to the culture media and treated for 24~48 hours ...
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bioRxiv - Immunology 2022Quote: ... 100ng/ml of recombinant human (rh) IL-8 (Biolegend, San Diego, CA) were added to the apical side of the enteroid monolayers (76) ...
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bioRxiv - Microbiology 2022Quote: ... in the presence of 50 ng/mL recombinant human M-CSF (Biolegend) for 7 days ...
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bioRxiv - Cancer Biology 2020Quote: ... in DMEM/F12 supplemented with human recombinant EGF (20 ng/mL, BioLegend), bFGF (20 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR-NK cells were loaded with human recombinant PD-L1- Fc (Biolegend) or ErBb2-Fc fusion proteins (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... and 200 ng/mL recombinant human B cell-activating factor (BAFF, BioLegend).
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/mL human recombinant IL-3 (BioLegend, San Diego, CA, USA) and antibiotics ...
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bioRxiv - Microbiology 2023Quote: ... in the presence of 50 ng/mL recombinant human M-CSF (Biolegend) for 7 days ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human NRG1 was acquired from BioLegend (San Diego, CA, Cat. 551904). High-throughput drug screening assays were used with compounds sourced as indicated in Suppl ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2019Quote: ... then incubated with 0.5 ml of human TruStain FcX Fc receptor blocking solution (Biolegend, San Diego, CA) for 10 minutes.
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2020Quote: ... and were cultured with 50 ng/ml recombinant human GM-CSF (BioLegend #572904) and 50 ng/ml recombinant human M-CSF (BioLegend #574806 ...
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bioRxiv - Bioengineering 2021Quote: ... and 20 ng/mL recombinant human IL-3 (BioLegend; San Diego, CA, USA) to determine cellular distribution ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then treated with 100 ng/ml of recombinant human IFNγ (Biolegend) for 24h ...
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bioRxiv - Cancer Biology 2022Quote: ... “Rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). Scratches were imaged at 0 hours ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human SARS-CoV-2 spike S1 IgG lyophilized antibody (Clone AM009105, BioLegend) was used as a standard.
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution, Cat# 422302, BioLegend) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The “rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). After 24 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were supplemented with 100 U/ml recombinant human IFN-γ (BioLegend) for 3 days to induce PD-L1 expression ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with different combinations of 400 ng/mL human recombinant sFasL (Biolegend), 40 μM Fasudil HCl (SellekChem) ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor was blocked with an anti-Fc receptor antibody (BioLegend). The cells were stained with a cocktail of antibodies against three surface makers ...
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bioRxiv - Cell Biology 2024Quote: ... Fc receptors were blocked with 10 µL TruStain FCX receptor block (BioLegend, 422301) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then incubated with Brilliant Violet 421-labelled anti-human P2Y12 receptor antibody (clone S16001E, Biolegend, Cat# 392106) or with IgG2a isotype control antibody (clone MOPC-173 ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked by incubating cells with human anti-Fc Receptor antibodies (TrueStain, Biolegend, Cat. No. 101320) or anti-mouse CD16/32 Fc block (Biolegend ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... In all assays 1 x 106 cells were first incubated with Human TruStain FcX (Fc Receptor Blocking Solution, Biolegend) or anti-human CD32 (FcγRII Blocker ...
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bioRxiv - Immunology 2022Quote: After thawing cells were washed and Fc receptors were blocked with TruStain FcX (mouse or human as appropriate; Biolegend) for 5 minutes at room temperature before staining with diluted antibodies (Supplemental Tables 2 ...
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bioRxiv - Immunology 2023Quote: ... CD141 BV605(Clone:M80 Cat:344117) in the presence of human anti-Fc Receptor antibodies (TrueStain; Biolegend; cat. no. 101320). cDCs1 were FACS-sorted as CD45+Lin- (Lin:CD3 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and staining for surface antigens was performed following Fc receptor blockade with Human TruStain FcX (BioLegend). Central/effector memory CD4+ T cells were defined as live/CD16-/CD14-/CD123-/CD56-/CD11c-/CD19-/CD4+ > excluding CD25hiCD127lo > CD45RA-/CD27+ ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal cultures were treated with 200 ng/mL recombinant human NRG1 (BioLegend, Cat. No. 551904) for 48 hrs ...
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bioRxiv - Immunology 2023Quote: ... 550 µl of RPMI medium supplemented with 1 µg/ml recombinant human CCL21 (BioLegend; #582208) or nothing as negative control were added to the lower chambers ...
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bioRxiv - Immunology 2024Quote: ... The ultrasound-guided intra-amniotic injection of recombinant human HMGB1 (Biolegend, San Diego, CA, USA) at a concentration of 9 ng dissolved in 100 μL of sterile 1X phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptor blockade (Biolegend 101319), and anti-CD31 antibody (BD Biosciences 561073) ...