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401 - 450 of 2691 citations for Recombinant Human IL4 None tagged since 2019

• Computational ranking-assisted identification of Plexin-B2 in homotypic and heterotypic clustering of circulating tumor cells in breast cancer metastasis

  Emma Schuster, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Sema4A PE (human/mouse, BioLegend #148404). Cells were then run through BD FACSMelody Cell Sorter and cells were collected based on gated populations ...
• Differential neural circuit vulnerability to β-amyloid and tau pathologies in novel Alzheimer’s disease mice

  Maria Dolores Capilla-López, et al., bioRxiv - Neuroscience 2023

  Quote: ... human Aβ (6E10) (BioLegend, SIG-39320), synaptophysin (Sigma ...
• Microglia at Sites of Atrophy Restrict the Progression of Retinal Degeneration via Galectin-3 and Trem2 Interactions

  Chen Yu, et al., bioRxiv - Immunology 2023

  Quote: ... BV510 anti-human CD11B (Biolegend #562950) and processed with single-cell multiplexing kit (BD) ...
• Solid tumor-on-chip model for efficacy and safety assessment of CAR-T cell therapy

  Tengku Ibrahim Maulana, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-human CD69 FITC (310904; BioLegend) and anti-human CD25 BV421 (302630 ...
• Eta-secretase-like processing of the amyloid precursor protein (APP) by RHBDL4

  Penalva Ylauna Christine Megane, et al., bioRxiv - Biochemistry 2023

  Quote: ... 6E10 (human Aβ region-specific, Biolegend), 2E9 (epitope as determined by Willem et al. ...
• Solid tumor-on-chip model for efficacy and safety assessment of CAR-T cell therapy

  Tengku Ibrahim Maulana, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-human CD8a PerCP (300922; BioLegend), anti-human CD69 FITC (310904 ...
• Loss of TMEM106B exacerbates Tau pathology and neurodegeneration in PS19 mice

  Tuancheng Feng, Huan Du, Fenghua Hu, bioRxiv - Neuroscience 2023

  Quote: ... rat anti-human Tau (BioLegend, A16103A), Phospho-Tau (Ser404 ...
• PD-1 signaling uncovers a pathogenic subset of T cells in inflammatory arthritis

  Johanna Straube, et al., bioRxiv - Immunology 2023

  Quote: ... mouse anti-human CD28 antibody (BioLegend), recombinant human PD-L1 human IgG1 Fc chimera protein (R&D Systems) ...
• SAP expressing T peripheral helper cells identify systemic lupus erythematosus patients with lupus nephritis

  Yevgeniya Gartshteyn, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-human SLAMF3 (BioLegend #326109).
• Lactose blocks intercellular spreading of Galectin-1 from cancer cells to T-cells and activates tumor immunological control

  Yu Hong, et al., bioRxiv - Immunology 2023

  Quote: ... APC anti-human CD8α (BioLegend, 300912), PE anti-human IFNγ (BioLegend ...
• Lactose blocks intercellular spreading of Galectin-1 from cancer cells to T-cells and activates tumor immunological control

  Yu Hong, et al., bioRxiv - Immunology 2023

  Quote: ... PE anti-human IFNγ (BioLegend, 506507), and FITC anti-mouse TCR Vα2 (BioLegend ...
• Emergence of a senescent and inflammatory pulmonary CD4⁺ T cell population prior to lung allograft failure

  Sajad Moshkelgosha, et al., bioRxiv - Immunology 2023

  Quote: ... APC-conjugated anti-human PD1 (BioLegend); PE/Cy7-conjugated anti-human CD57 ...
• Targetable leukemia dependency on noncanonical PI3Kγ signaling

  Qingyu Luo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... FITC anti-human CD14 (325604; BioLegend), and APC/Cyanine7 anti-mouse/human CD11b (101226 ...
• Metabolic Deficiencies Underlie Plasmacytoid Dendritic Cell Exhaustion After Viral Infection

  Trever T. Greene, et al., bioRxiv - Immunology 2024

  Quote: ... anti-human CD56 (MEM-188, BioLegend), HLA-DR (L243 ...
• Combining Cell-Intrinsic and -Extrinsic Resistance to HIV-1 By Engineering Hematopoietic Stem Cells for CCR5 Knockout and B Cell Secretion of Therapeutic Antibodies

  William N. Feist, et al., bioRxiv - Bioengineering 2024

  Quote: ... anti-human IgD (BioLegend, cat.: 348232), and anti-human IgM (BD ...
• Combining Cell-Intrinsic and -Extrinsic Resistance to HIV-1 By Engineering Hematopoietic Stem Cells for CCR5 Knockout and B Cell Secretion of Therapeutic Antibodies

  William N. Feist, et al., bioRxiv - Bioengineering 2024

  Quote: ... anti-human CD27 (BioLegend, cat.: 302832), anti-human CD38 (BD ...
• Combining Cell-Intrinsic and -Extrinsic Resistance to HIV-1 By Engineering Hematopoietic Stem Cells for CCR5 Knockout and B Cell Secretion of Therapeutic Antibodies

  William N. Feist, et al., bioRxiv - Bioengineering 2024

  Quote: ... anti-human CD3 (Biolegend, cat.: 300328), and viability dye Ghost Dye™ Violet 540 (Tonbo Bioscience ...
• Combining Cell-Intrinsic and -Extrinsic Resistance to HIV-1 By Engineering Hematopoietic Stem Cells for CCR5 Knockout and B Cell Secretion of Therapeutic Antibodies

  William N. Feist, et al., bioRxiv - Bioengineering 2024

  Quote: ... anti-human CD19 (Biolegend, cat.: 302212), anti-human CD3 (Biolegend ...
• Combining Cell-Intrinsic and -Extrinsic Resistance to HIV-1 By Engineering Hematopoietic Stem Cells for CCR5 Knockout and B Cell Secretion of Therapeutic Antibodies

  William N. Feist, et al., bioRxiv - Bioengineering 2024

  Quote: ... anti-human CD45 (Biolegend, cat.: 368540), anti-human HLA-ABC (Biolegend ...
• A longevity-specific bank of induced pluripotent stem cells from centenarians and their offspring

  Todd W. Dowrey, et al., bioRxiv - Molecular Biology 2024

  Quote: ... blocked with Human FcBlock (BioLegend 422301) and stained with a panel of 40 fluorescent reagents (Supplementary Table 1 ...
• AXL limits the mobilization of cholesterol to regulate dendritic cell maturation and the immunogenic response to cancer

  Meriem Belabed, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human CD83 (clone HB15e, BioLegend), anti-human CD86 (clone BU63 ...
• AXL limits the mobilization of cholesterol to regulate dendritic cell maturation and the immunogenic response to cancer

  Meriem Belabed, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human CD86 (clone BU63, BioLegend), anti-human PD-L1 (clone 29E2A3 ...
• AXL limits the mobilization of cholesterol to regulate dendritic cell maturation and the immunogenic response to cancer

  Meriem Belabed, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human CD1C (clone L161, BioLegend), anti-human CD123 (clone 6H6 ...
• AXL limits the mobilization of cholesterol to regulate dendritic cell maturation and the immunogenic response to cancer

  Meriem Belabed, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human CD80 (clone W17149D, BioLegend), anti-human CD83 (clone HB15e ...
• AXL limits the mobilization of cholesterol to regulate dendritic cell maturation and the immunogenic response to cancer

  Meriem Belabed, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human CD123 (clone 6H6, BioLegend), anti-human CLEC9A (clone 8F9 ...
• AXL limits the mobilization of cholesterol to regulate dendritic cell maturation and the immunogenic response to cancer

  Meriem Belabed, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human CLEC9A (clone 8F9, BioLegend), anti-human CADM1 (clone 30 ...
• AXL limits the mobilization of cholesterol to regulate dendritic cell maturation and the immunogenic response to cancer

  Meriem Belabed, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human CD45RA (clone HI100, BioLegend), anti-human CD1C (clone L161 ...
• Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets

  Jeong Min Lee, et al., bioRxiv - Biochemistry 2024

  Quote: ... and anti-human CD3 (BioLegend, # 300420). Samples were acquired and recorded on a BD FACSAria II and data were analyzed with FlowJo™ software.
• Plasma Glycomic Markers of Accelerated Biological Aging During Chronic HIV Infection

  Leila B Giron, et al., bioRxiv - Microbiology 2023

  Quote: ... TotalSeq-B human CITEseq antibodies (BioLegend) used to subtype immune cells in the downstream data were ...
• Distinct cell adhesion signature defines glioblastoma myeloid-derived suppressor cell subsets

  Defne Bayik, et al., bioRxiv - Immunology 2021

  Quote: Sorted mMDSCs (50,000) were stimulated with 50 ng/ml recombinant mouse M-CSF (Biolegend) in Iscove’s Modified Dulbecco’s Medium (IMDM ...
• Pathogenic, but not non-pathogenic, Rickettsia evade inflammasome-dependent IL-1 responses to establish an intracytosolic replication niche

  Oliver H. Voss, et al., bioRxiv - Microbiology 2022

  Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.
• Interleukin-6 Drives Key Pathologic Outcomes in Experimental Acetaminophen-induced Liver Failure

  Katherine Roth, et al., bioRxiv - Pathology 2021

  Quote: ... mice were injected with 5 μg recombinant mouse IL-10 (Biolegend, San Diego, CA) or sterile saline 24 hours after APAP challenge ...
• Reduced cutaneous CD200:CD200R1 signalling in psoriasis enhances neutrophil recruitment to skin

  Holly Linley, et al., bioRxiv - Immunology 2022

  Quote: Mouse dorsal skin cells were stimulated with 40 ng/ml recombinant IL-23 (Biolegend) for 20 hr ...
• Cooperative phagocytosis underlies macrophage immunotherapy of solid tumours and initiates a broad anti-tumour IgG response

  Jason C. Andrechak, et al., bioRxiv - Bioengineering 2022

  Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend, 576406). Successful differentiation was confirmed by flow cytometry staining with antibodies against macrophage markers ...
• Antibody-lectin chimeras for glyco-immune checkpoint blockade

  Jessica C. Stark, et al., bioRxiv - Biochemistry 2022

  Quote: ... and cells were cultured overnight with 0.5 μg/mL recombinant IL-2 (Biolegend 589106) in RPMI + 10% heat-inactivated FBS until use.
• Titrating CD47 by mismatch CRISPRi reveals incomplete repression can eliminate IgG-opsonized tumors but CD47 heterogeneity limits induction of anti-tumor IgG

  Brandon H. Hayes, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...
• Interferon-induced lysosomal membrane permeabilization and death cause cDC1-deserts in tumors

  E. Aerakis, et al., bioRxiv - Immunology 2023

  Quote: MutuDC1 cells were treated with mouse recombinant 20ng/ml IFN-β1 (Biolegend Cat.581302), mouse 80ng/ml IFN-α (Miltenyi Cat ...
• Elevated CD153 Expression on Aged T Follicular Helper Cells is Vital for B cell Responses

  Alyssa L. Thomas, et al., bioRxiv - Immunology 2023

  Quote: ... in the presence or absence of 20ng/ml of recombinant mouse IL-6 (Biolegend) and/or 200ng/ml of nivalenol (Cayman Chemical ...
• Data-driven discovery of innate immunomodulators via machine learning-guided high throughput screening

  Yifeng Tang, et al., bioRxiv - Immunology 2023

  Quote: ... Recombinant Mouse GM-CSF (carrier-free; 20 ng/ml; BioLegend, San Diego, California, USA), 2 mM L-glutamine (Life Technologies) ...
• N-glycosylation engineering in chimeric antigen receptor T cells enhances anti-tumor activity

  Elien De Bousser, et al., bioRxiv - Immunology 2023

  Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
• Temporal perturbation of STAT1/2 activity reveals dynamic ligand discrimination of type I interferon signaling

  Haowen Yang, et al., bioRxiv - Systems Biology 2023

  Quote: ... followed by stimulation with recombinant mouse IFNα or IFNβ1 (BioLegend Uk Limited, London, UK) at each condition.
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: ... under Th1-polarizing condition (10 ng/mL recombinant mouse IL-12 (p70) (#577004, BioLegend), and 10 μg/mL anti-mouse IL-4 antibody (clone 11B11 ...
• Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

  Yin Luo, et al., bioRxiv - Cell Biology 2023

  Quote: MAD-MB-453 cells were treated with recombinant hTRAIL (30 ng/ml, B294007, Biolegend) for 6 h ...
• Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response

  Brandon H. Hayes, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...
• NLRP3 inflammasome contributes to host defense against Talaromyces marneffei infection

  Haiyan Ma, et al., bioRxiv - Microbiology 2020

  Quote: ... IL-18 and IFN-γ from human PBMCs were measured by LEGENDplex™ Human Inflammation Panel (Biolegend) according to manufacturer’s instructions.
• CARD8 inflammasome mediates pyroptosis of HIV-1-infected cells by sensing viral protease activity

  Qiankun Wang, et al., bioRxiv - Immunology 2020

  Quote: ... Human CD14+ cells were isolated from healthy donor PBMCs using MojoSort Human CD14 Selection Kit (Biolegend #480026), and were cultured with 50 ng/ml recombinant human GM-CSF (BioLegend #572904 ...
• A molecular switch between mammalian MLL complexes dictates response to Menin-MLL inhibition

  Yadira M. Soto-Feliciano, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and stained with anti-human CD45 (PE) and anti-human CD117 (APC) (Biolegend, San Diego, CA, USA). CD45-low/CD117+ leukemia cells were FACS sorted (MA900 sorter ...
• YB-1 IS REQUIRED FOR THE GENESIS AND METASTATIC CAPACITY OF HUMAN BREAST CANCER

  Sylvain Lefort, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Human cells were sorted after staining with anti-human specific antibodies directed against EpCAM and CD298 (Biolegend) with simultaneous depletion of mouse cells stained with anti-mouse-specific antibodies directed against CD45 and CD31 (Biolegend).
• HDAC inhibitors decrease TLR7/9-mediated human plasmacytoid dendritic cell activation by interfering with IRF-7 and NF-κB signaling

  Dante B. Descalzi-Montoya, et al., bioRxiv - Immunology 2020

  Quote: ... anti-human BDCA-2-PE (clone AC144; Miltenyi Biotech) and anti-human CD123-APC (clone 6H6; Biolegend). Cells were incubated with surface antibodies at 4°C for 30 minutes ...
• Modified E2 glycoprotein of hepatitis C virus enhances pro-inflammatory cytokines and protective immune response in mice

  Vijayamahantesh, et al., bioRxiv - Microbiology 2022

  Quote: Isolated CD4+T cells from healthy donors were incubated with sE2 or sE2F442NYT in the presence of 5µg/ml human anti-CD3 antibody and 1µg/ml human anti-CD28 antibody (BioLegend) for 2 hrs ...