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Citations for BioLegend :
301 - 350 of 3175 citations for Recombinant Human IL13RA1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice and resuspended in PBS containing 2% FBS before staining in the presence of Fc block (Miltenyi 130-059-901) with anti-human CD45 Alexa700 (clone HI30, BioLegend cat #304024) and anti-mouse CD45.1 PE-Cy7 (clone A20 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were blocked using an Fc blocking antibody (Biolegend Fc-block clone: 2.4G2) and lymphocytes were stained with CD3 and CD4 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... incubated with Fc Block (BioLegend) then with fluorochrome-conjugated antibodies (BioLegend unless noted ...
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bioRxiv - Immunology 2022Quote: ... anti-IgG Fc (Biolegend; M1310G05), anti-CD27 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Fc-blocking reagent (Biolegend, 422301) was used to block Fc receptor-introduced non-specific staining ...
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bioRxiv - Microbiology 2022Quote: ... CD16/32 (Fc block-Biolegend), B220 (AF700-Biolegend) ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptor blockade (Biolegend 101319), and anti-CD31 antibody (BD Biosciences 561073) ...
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bioRxiv - Genomics 2024Quote: ... Fc-blocking reagent (BioLegend, 422301) was used to block Fc receptor-introduced nonspecific staining ...
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bioRxiv - Cell Biology 2023Quote: ... or by consecutive cell sorting for the expression of fluorescent proteins (mCherry-tagged or mVenus-tagged constructs) or the re-expression of TLR2 at the cell surface (Clone T2.5, Biolegend 121810) until the cell population was homogeneous ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and tetramerized using fluorescently tagged streptavidin from Biolegend. 5,6-Dimethylxanthenone-4-acetic Acid (DMXAA ...
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bioRxiv - Immunology 2021Quote: ... metal-tagged antibodies including pan-keratin (C11, Biolegend), BDCA2 (Polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... Fc receptors were blocked with 0.5% anti-CD16/32 Fc block antibody (Biolegend, clone 93) diluted in PBS containing 2% goat serum (Vector Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... coverslips of each batch were incubated with 30 µg/ml anti-human IgG Fc (cross-reacts with rabbit) conjugated to Alexa Fluor-488 (Cat# 409321; BioLegend, San Diego, CA) for 45 minutes at room temperature in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Immunology 2022Quote: ... and APC anti-His Tag antibody (Biolegend) for 30 min each ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant galectin-3 (Biolegend # 599706) was then incubated either for 1-2 hrs at RT or overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... recombinant murine G-CSF (BioLegend), recombinant murine ICAM-1 (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... Reagents: recombinant murine CXCL1 (BioLegend), recombinant murine SCF (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant murine IL-4 (Biolegend) was combined with rat IgG1 mAb to IL-4 ...
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bioRxiv - Immunology 2020Quote: Recombinant murine IL-33 (Biolegend) was injected intraperitoneally to C57BL/6 mice (100 ng/mouse) ...
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bioRxiv - Immunology 2021Quote: ... Carrier-free recombinant mouse (Biolegend) or human (SinoBiological ...
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bioRxiv - Immunology 2022Quote: ... recombinant mouse IL2 (Biolegend #575408) and recombinant murine GMCSF (Peprotech #315-03 ...
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bioRxiv - Immunology 2023Quote: ... and recombinant IL-17A (BioLegend). Streptavidin HRP (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... recombinant murine IFN-γ (Biolegend) or PBS was administered by IP injection at 0.25 mg/kg on days 3-8 of DSS exposure ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant BMP6 (rBMP6) (Biolegend, USA), recombinant Follistatin (rFST ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant Follistatin (rFST) (Biolegend, USA) or vehicle control were added to the desired concentration whilst still preconfluent (approximately 24 hours before confluency) ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Biochemistry 2022Quote: Moles of product formed in hydrolysis reactions used for ACE2 activity and specificity profiling were determined by comparing area under the curve for LC chromatograms corresponding to these reactions to areas under the curve observed for control reactions in which > 95% input substrate conversion was achieved by incubation of substrate with 25 nM recombinant human ACE2 commercial standard (BioLegend, San Diego, CA) for between one and four hours pending peptide substrate ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with M-CSF (100ng/ml) Recombinant Human M-CSF (Macrophage-Colony Stimulating Factor, BioLegend cat. 574806, San Diego, CA, USA) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Developmental Biology 2021Quote: ... and then Fc blocked (BioLegend, 101320), before staining with an antibody cocktail containing anti-MPO (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... and Fc receptor blocking solution (Biolegend), before staining with fluorochrome-conjugated anti-human antibodies specific to anti-CD3 (UCHT1)/anti-CD45 (2D1) ...
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bioRxiv - Neuroscience 2022Quote: ... After fragment crystallizable (Fc) blocking (Biolegend), cells were stained for surface antigens (Extended Data Table 4) ...
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bioRxiv - Cancer Biology 2020Quote: ... After adding Fc receptor blocker (BioLegend), cells were labeled overnight at 4°C with a cocktail of antibodies recognizing chromatin modifications or intracellular components ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block (BioLegend), followed by tetramer incubation (25μg/ml ...
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bioRxiv - Immunology 2022Quote: ... 5µg/ml CD80-Fc (555404, Biolegend), and 2µg/ml PD-L1-Fc (758206 ...
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bioRxiv - Immunology 2022Quote: ... stained with Fc receptor block (BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... performed with Fc blocking (422302, BioLegend), and labelled with anti-CD138 (142508 ...
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bioRxiv - Immunology 2023Quote: ... After Fc blocking (TruStain FcX; BioLegend), cells were stained with a metal-conjugated surface antibody cocktail (Table 2) ...
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bioRxiv - Immunology 2024Quote: ... and incubated with Fc Block (Biolegend) for 10 min at room temperature ...