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Citations for BioLegend :
351 - 400 of 3648 citations for Recombinant Human EPCAM Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The ultrasound-guided intra-amniotic injection of recombinant human HMGB1 (Biolegend, San Diego, CA, USA) at a concentration of 9 ng dissolved in 100 μL of sterile 1X phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... 550 µl of RPMI medium supplemented with 1 µg/ml recombinant human CCL21 (BioLegend; #582208) or nothing as negative control were added to the lower chambers ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Genomics 2020Quote: ... Fc receptors were blocked by incubating the cells with 50 μl Human TruStain FcX (Biolegend, #422302) for 10 minutes at room temperature prior to antibody staining and sorting ...
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bioRxiv - Immunology 2022Quote: ... Whole blood cell staining was performed using an Fc receptor blocking solution (Human TruStain FcX; BioLegend), the viability dye eFluor780 (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Cells were pelleted again and resuspended in 100 ul of Fc-Block (Human TruStain FcX, Biolegend), and 10 ul aliquot was taken for counting using K2 Cellometer (Nexcelom ...
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bioRxiv - Immunology 2022Quote: ... followed by rat anti-human Fc antibody conjugated to APC (Cat: 410712, BioLegend; 1:100 dilution) for 15 min on ice in the dark for secondary detection ...
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bioRxiv - Immunology 2019Quote: ... suspended in PBS containing 1% BSA and an Fc receptor-blocking (human TruStainFcX; Biolegend, CA, USA). The neutrophils were then permeabilized with 0.1% saponin and incubated with a mAb against TLR9 conjugated with FITC (catalog number ab134369/clone 26C593.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 1 μg/mL of phycoerythrin (PE)-conjugated anti-human IgG Fc (BioLegend). Cells were washed once and resuspended in ice-cold FACS buffer ...
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bioRxiv - Microbiology 2022Quote: ... blocked with Fc Block for 20 min at room temperature (Human TruStain FcX, Biolegend Cat# 422302), and simultaneously stained for surface antigenic Site Ø ...
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bioRxiv - Immunology 2023Quote: Zombie Aqua Fixable Viability Dye and Human TruStain FcX Fc Receptor Blocking Solution were from BioLegend. CF700-SE and CF750-SE were bought from Biotium ...
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bioRxiv - Immunology 2020Quote: ... Recombinant mouse (rm) M-CSF and GM-CSF proteins were obtained from BioLegend. MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK ...
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bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...
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bioRxiv - Bioengineering 2021Quote: ... such as the recombinant human matrix metalloproteinases 12 (MMP12, 1 μl, 20 ng/ml, Biolegend, U.S.A), MMP408 (the inhibitor of MMP12 ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured in 10 ng/mL recombinant human GM-CSF or M-CSF (both Biolegend) for 7 days at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured in 10 ng/mL recombinant human GM-CSF or M-CSF (both Biolegend) for 7 days at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained for EpCAM+ FITC (Biolegend, 118208, G8.8, 1:20) and TPC markers CD24 PerCP-eFluor 710 (eBioscience ...
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bioRxiv - Cell Biology 2022Quote: ... and FITC-conjugated anti-Epcam antibody (1:500 dilution, 118208, Biolegend), washed twice with IESC media and DAPI (0.1 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... or rat anti-epithelial cell adhesion molecule (EpCAM; clone G8.8, BioLegend). After washing ...
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bioRxiv - Immunology 2022Quote: ... and then stained with the following antibodies: EpCAM-BV785 (BioLegend #118245), CD45-APC (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... Pluripotency was examined via expression of EPCAM-BV421 (Biolegend, Cat. 324220), CD9-FITC (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... EpCAM–APC (eBioscience, G8.8) and CD24–Pacific Blue (Biolegend, M1/69), all diluted 1:250 ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were stained with antibodies specific for EpCAM (BioLegend), CD45 (eBioscience) ...
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bioRxiv - Immunology 2024Quote: ... and collected cells were stained with PE-anti-EPCAM (Biolegend 324206) and Alexa488-anti-CD45 (Biolegend 304017 ...
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bioRxiv - Immunology 2023Quote: Antigen-specific B cell subsets were detected using biotinylated SARS-CoV-2 RBD protein (793906; Biolegend) in combination with streptavidin (SAv)-fluorophore conjugates as described [7] ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... then incubated with 0.5 ml of human TruStain FcX Fc receptor blocking solution (Biolegend, San Diego, CA) for 10 minutes.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in MACS buffer and incubated with PE–anti-human FC antibody (Biolegend, M1310G05) in MACS buffer for 30 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc-block was performed by incubating cells in a dilution of Human TruStain FcX (#422302, BioLegend, USA) and mouse TruStain FcX (#101320 ...
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bioRxiv - Genomics 2023Quote: ... The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend) for 10 min at 4°C and then with fixable viability dye DAPI (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Bioengineering 2023Quote: ... The 5x diluted streptavidin-coated nanovial suspension was then incubated with an equal volume of 71.5 μg/mL of biotinylated anti-human VEGF antibody (Biolegend) for 30 minutes at room temperature on a tube rotator and washed ...
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bioRxiv - Microbiology 2022Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.
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bioRxiv - Developmental Biology 2021Quote: ... EpCAM-APC/Cy7 (Biolegend, clone G8.8, 0.5 µg ml−1, 1:500), Cd49f-BV421 (Biolegend 313623 ...
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bioRxiv - Cancer Biology 2020Quote: ... endothelial cells (CD31) and epithelial cells (EpCAM clone G8.8; all from Biolegend).
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bioRxiv - Cell Biology 2022Quote: ... PE-conjugated rat anti-mouse EpCam antibody (1:500, BioLegend, G8.8, #118206) for 45 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... anti-dsRNA (J2, 10010, Scicons) or anti-EpCAM-AF488 (9C4, 324210, BioLegend), for 1 hour in PBS with 1% BSA media at room temperature ...
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bioRxiv - Physiology 2021Quote: ... antibody and APC anti-mouse CD326 (EpCAM) antibody (clone G8.8, 118214, Biolegend) were used in a 1:50 and 1:100 dilution ratio ...
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bioRxiv - Immunology 2020Quote: ... We used the Anti-EpCam-PE (Clone: G8.8, Biolegend, San Diego, USA) antibody for analysis of IEC purity ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were stained for 20 minutes Epcam-APC (1:200, BioLegend 118214), washed and re-suspended in media containing DAPI (1:10’000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Further antibody incubation was performed using CD45-FTIC and EpCAM-APC (Biolegend), in a total volume of 100 μl volume ...
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bioRxiv - Cell Biology 2023Quote: ... Cell suspensions were then stained with 1:200 PE-EpCAM (BioLegend 118205), 1:200 FITC-CD45 (BioLegend 103107) ...
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bioRxiv - Developmental Biology 2023Quote: ... BV421 rat anti-epithelial cell adhesion molecule (EPCAM, 1:250, 118225, BioLegend), and AF647 rat anti-Intercellular adhesion molecule 2 (ICAM2 ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice after the primary stain and incubated with PE–anti-human Fc antibody (Biolegend, 410708) in MACS buffer for 20 min on ice ...