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301 - 350 of 2894 citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019

• Killing them softly with EPO: a new role for erythropoietin in the homeostasis of red blood cells

  Clemente F. Arias, et al., bioRxiv - Physiology 2023

  Quote: ... with or without EPO (35U/well) (recombinant mouse EPO carrier-free, Biolegend). The ratio of splenocytes to RBCs was 1:1 ...
• Bosutinib stimulates macrophage survival, phagocytosis and intracellular killing of bacteria

  Ronni A. G. da Silva, et al., bioRxiv - Microbiology 2023

  Quote: ... plated in complete RPMI with 50 ng/mL recombinant M-CSF (Biolegend) and cultured for 6 days with medium change every 3 days.
• Blocking MIF secretion enhances CAR T-cell efficacy against neuroblastoma

  Josephine G. M. Strijker, et al., bioRxiv - Cancer Biology 2024

  Quote: ... in the presence of 10 ng/mL recombinant MIF (Biolegend, cat. 599404) or MDK (Biolegend ...
• Immunological role of primary cilia of dendritic cells in human skin disease

  Manami Toriyama, et al., bioRxiv - Cell Biology 2020

  Quote: Human PBMCs were labeled with Alexa 647 anti-human CD11c (301620, clone 3.9, BioLegend), anti-human HLA-DR (Class III ...
• Single cell transcriptome mapping identifies a local innate B cell population driving local antibody production and chronic rejection after lung transplantation

  Natalia F. Smirnova, et al., bioRxiv - Physiology 2021

  Quote: Immunoglobulins from human BALF were measured with the LEGENDplexTM Human Immunoglobulin Isotyping panel (BioLegend), according to the manufacturer’s instructions.
• The half-life of the bone-derived hormone osteocalcin is regulated through O-glycosylation in mice, but not in humans

  Omar Al Rifai, et al., bioRxiv - Biochemistry 2020

  Quote: ... Human ucOCN measurements were performed using a commercially available human ucOCN ELISA (BioLegend, 446707) (Lacombe et al. ...
• Targetable leukemia dependency on noncanonical PI3Kγ signaling

  Qingyu Luo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Human leukemia cells were labelled with a FITC anti-human CD45 antibody (368508; BioLegend) then sorted using a BD FACS Aria II Cell Sorter.
• PTPRZ1-targeting RNA CAR-T cells exert antigen-specific and bystander antitumor activity in glioblastoma

  Darel Martinez Bedoya, et al., bioRxiv - Immunology 2023

  Quote: ... samples were stained with anti-human HLA-ABC and anti-human CD3PE (BioLegend, 317308) at RT in the dark ...
• A general platform for targeting MHC-II antigens via a single loop

  Haotian Du, et al., bioRxiv - Bioengineering 2024

  Quote: ... followed by the staining with anti-His-APC (1:20, clone J095G46, BioLegend cat# 362605) with Fc block (1:20 ...
• Longitudinal analysis of invariant natural killer T cell activation reveals a cMAF-associated transcriptional state of NKT10 cells

  Harry Kane, et al., bioRxiv - Immunology 2021

  Quote: ... adipose and/or splenic iNKT cell samples were first tagged by TotalSeq-A Mouse hashtag antibodies (Biolegend) and then pooled for sequencing ...
• Rapid, direct detection of bacterial Topoisomerase 1-DNA adducts by RADAR/ELISA

  Devapriya Sinha, et al., bioRxiv - Microbiology 2020

  Quote: ... Primary detection of TRX-tagged YpTop1 was with primary murine monoclonal anti-TRX (BioLegend #658902, 1μg/ml), ...
• An endoribonuclease-based feedforward controller for decoupling resource-limited genetic modules in mammalian cells

  Ross D. Jones, et al., bioRxiv - Synthetic Biology 2020

  Quote: HA-tagged Gal4 TAs were stained with anti-HA.11 directly conjugated to Alexa Fluor 594 (BioLegend catalogue #901511 ...
• A single episode of CMV reactivation initiates long-term expansion and differentiation of the NK cell repertoire

  Norfarazieda Hassan, et al., bioRxiv - Immunology 2022

  Quote: ... anti-human CD57-PB (BioLegend, Cat#322316] ...
• Microtubule assembly by soluble tau impairs vesicle endocytosis and excitatory neurotransmission via dynamin sequestration in Alzheimer’s disease synapse model

  Tetsuya Hori, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-human Tau (BioLegend, #806501), anti-dynamin (Invitrogen ...
• The Ca²⁺ concentration in vitro impacts the cytokine production of mouse and human lymphoid cells and the polarization of human macrophages

  Yusuf Cem Eskiocak, et al., bioRxiv - Immunology 2021

  Quote: ... or Human TruStain FcX (BioLegend) was used to block Fc receptors ...
• Familial t(1;11) translocation is associated with disruption of white matter structural integrity and oligodendrocyte-myelin dysfunction

  Navneet A. Vasistha, et al., bioRxiv - Neuroscience 2019

  Quote: ... Human GFAP (1:1000, Biolegend), Neurofilament-H (1:10,000 ...
• Autologous humanized mouse models of iPSC-derived tumors allow for the evaluation and modulation of cancer-immune cell interactions

  Gaël Moquin-Beaudry, et al., bioRxiv - Cancer Biology 2021

  Quote: ... human CD8 (Biolegend, clone HIT8a), SV40 LargeT antigen (Santa Cruz ...
• Targeting Chromatin Effector Pygo2 to Enhance Immunotherapy in Prostate Cancer

  Yini Zhu, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and human CD8a (Biolegend, 372902).
• Identification of HIV-Reservoir Cells with Reduced Susceptibility to Antibody-Dependent Immune Response

  Antonio Astorga-Gamaza, et al., bioRxiv - Immunology 2022

  Quote: ... anti-human CD20 (BV785, Biolegend), and anti-human HLA-DR (BV711 ...
• Identification of HIV-Reservoir Cells with Reduced Susceptibility to Antibody-Dependent Immune Response

  Antonio Astorga-Gamaza, et al., bioRxiv - Immunology 2022

  Quote: ... anti-human CD3 (AF700, Biolegend), anti-human CD32 (PE-Cy7 ...
• B cells engineered to express an anti-HIV antibody allow memory retention, class switch recombination and clonal selection in mice

  Alessio D. Nahmad, et al., bioRxiv - Immunology 2020

  Quote: BV421 anti-human IgK (Biolegend)
• Potent Neutralizing Antibodies Directed to Multiple Epitopes on SARS-CoV-2 Spike

  Lihong Liu, et al., bioRxiv - Microbiology 2020

  Quote: ... and human Hashtag 3 (Biolegend) at 4°C for 1 hr ...
• Public and private human T cell clones respond differentially to HCMV antigen when boosted by CD3 co-potentiation

  Laura R.E. Becher, et al., bioRxiv - Immunology 2020

  Quote: ... anti-human CD45 (BioLegend, UCHL1), anti-human CD56 (BD Biosciences ...
• Public and private human T cell clones respond differentially to HCMV antigen when boosted by CD3 co-potentiation

  Laura R.E. Becher, et al., bioRxiv - Immunology 2020

  Quote: ... anti-human CD19 (BioLegend, HIB19), anti-Vβ5 (BioLegend ...
• Long-lasting germinal center responses to a priming immunization with continuous proliferation and somatic mutation

  Jeong Hyun Lee, et al., bioRxiv - Immunology 2021

  Quote: ... anti-human hashtag antibodies (Biolegend) were individually added to each sample at a concentration of 2.5 µg/ 5 million cells at the time of addition of the master mix ...
• MAFB surrogates the glucocorticoid receptor ability to induce tolerogenesis in dendritic cells

  Octavio Morante-Palacios, et al., bioRxiv - Immunology 2021

  Quote: ... and Human TNFα from BioLegend, and Human IL-1β from ThermoFisher.
• Early human lung immune cell development and its role in epithelial cell fate

  Josephine L. Barnes, et al., bioRxiv - Immunology 2022

  Quote: ... using Human TruStain FcX (Biolegend) and then stained with FACS antibodies (Table S5) ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD4 (anti-human APC, Biolegend), and CD8 (anti-human PerCP-Cy5.5 ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD43 (anti-human PE, Biolegend), and CD45 (anti-human APC ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD11b (anti-human APC, Biolegend), CXCR3 (anti-human BV650 ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CXCR3 (anti-human BV650, Biolegend), CD45 (anti-human Alexa Fluor 700 ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD206 (anti-human BV785, Biolegend), and CD14 (anti-human APC Fire 750 ...
• B-Cell Precursor Acute Lymphoblastic Leukemia elicits an Interferon-α/β response in Bone Marrow-derived Mesenchymal Stroma

  Mandy W. E. Smeets, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PE anti-human CD45 (Biolegend), Alexa Fluor 750-conjugated human 5’-Nucleotidase/CD73 ...
• Activated interstitial macrophages are a predominant target of viral takeover and focus of inflammation in COVID-19 initiation in human lung

  Timothy Ting-Hsuan Wu, et al., bioRxiv - Genomics 2023

  Quote: ... anti-human CD45 (BioLegend 324016), CD204 (BioLegend 371904) ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... human IgM-AF700 (BioLegend, 314537), human IgD-perCP/Cy5.5 (BioLegend ...
• CD32 allows capturing blood cells emergence in slow motion during human embryonic development

  Rebecca Scarfò, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-human CD32 (Biolegend, 303202) and rabbit anti-human/mouse Runx1 (Abcam ...
• B-Cell Precursor Acute Lymphoblastic Leukemia elicits an Interferon-α/β response in Bone Marrow-derived Mesenchymal Stroma

  Mandy W. E. Smeets, et al., bioRxiv - Cancer Biology 2023

  Quote: ... BV650 anti-human CD16 (Biolegend), BUV395 anti-human CD19 and CD123 (BD Biosciences ...
• B-Cell Precursor Acute Lymphoblastic Leukemia elicits an Interferon-α/β response in Bone Marrow-derived Mesenchymal Stroma

  Mandy W. E. Smeets, et al., bioRxiv - Cancer Biology 2023

  Quote: ... BV605 anti-human CD45 (Biolegend), APC anti-human CD66b ...
• Targeted therapies prime oncogene-driven lung cancers for macrophage-mediated destruction

  Kyle Vaccaro, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or Human TruStain FcX (BioLegend). The following antibodies were used for FACS analysis ...
• SAP expressing T peripheral helper cells identify systemic lupus erythematosus patients with lupus nephritis

  Yevgeniya Gartshteyn, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human SLAMF6 (BioLegend #317202) and anti-human SLAMF3 (BioLegend #326109).
• SARS-CoV-2 spike protein induces the cytokine release syndrome by stimulating T cells to produce more IL-2

  Chao Niu, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human IL-6 (Biolegend), or isotype control antibodies for 30 min.
• Human pluripotent stem cell-derived respiratory airway progenitors generate alveolar epithelial cells and recapitulate features of idiopathic pulmonary fibrosis

  Mikael G. Pezet, et al., bioRxiv - Cell Biology 2023

  Quote: ... Conjugated human EPCAM antibody (Biolegend) was added for 30 minutes ...
• Metabolic Deficiencies Underlie Plasmacytoid Dendritic Cell Exhaustion After Viral Infection

  Trever T. Greene, et al., bioRxiv - Immunology 2024

  Quote: ... anti-human CD19 (HIB19, BioLegend), anti-human CD56 (MEM-188 ...
• Disrupting CD38-driven T cell dysfunction restores sensitivity to cancer immunotherapy

  Or-Yam Revach, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Human TrueStain FcX (Biolegend, 422302) or mouse FcR blocker (Miltenyi 130-092-575 ...
• Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets

  Jeong Min Lee, et al., bioRxiv - Biochemistry 2024

  Quote: ... anti-human CD19 (BioLegend, # 302212), anti-human CD33 (BioLegend ...
• Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets

  Jeong Min Lee, et al., bioRxiv - Biochemistry 2024

  Quote: ... anti-human CD33 (BioLegend, # 366608), anti-human CD34 (BioLegend ...
• Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets

  Jeong Min Lee, et al., bioRxiv - Biochemistry 2024

  Quote: ... anti-human CD235a (BioLegend, # 349104), anti-human CD19 (BioLegend ...
• Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets

  Jeong Min Lee, et al., bioRxiv - Biochemistry 2024

  Quote: ... anti-human CD34 (BioLegend, # 343504), and anti-human CD3 (BioLegend ...
• In vivo single cell transcriptomics reveals Klebsiella pneumoniae rewiring of lung macrophages to promote infection

  Amy Dumigan, et al., bioRxiv - Microbiology 2021

  Quote: THP-1 human macrophages were treated with mouse anti-human Fc block (Ref: 422302, BioLegend), for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend) ...
• Human immunocompetent Organ-on-Chip platforms allow safety profiling of tumor-targeted T-cell bispecific antibodies

  S. Jordan Kerns, et al., bioRxiv - Cancer Biology 2021

  Quote: ... which consisted of anti-human Alexa Fluor® 700 anti-human CD3 (BioLegend, cat. 300324), Brilliant Violet 785™ anti-human CD4 (BioLegend ...