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Citations for BioLegend :
51 - 100 of 3642 citations for Recombinant Human CD4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... and PE/Cyanine 7 anti-human CD4 antibody (#317414, Biolegend), APC/Cyanine 7 anti-human CD8 antibody (#344714 ...
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bioRxiv - Cancer Biology 2021Quote: ... Brilliant Violet 785™ anti-human CD4 (BioLegend, cat. 317442) and Brilliant Violet 650™ anti-human CD69 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Immunology 2021Quote: ... and 5ng/ml recombinant human G-CSF (BioLegend). The cells were then incubated for 16 hours at 37°C and 5%CO2 ...
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bioRxiv - Immunology 2020Quote: ... Human recombinant IFN-α2 was from Biolegend (592704) and hybrid IFNα-B/D was from Novartis (CGP35269).
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bioRxiv - Developmental Biology 2020Quote: ... 100 ng/ml recombinant human IGF-1 (BioLegend), 50 ng/ml recombinant human FGF-basic (FGF-2 ...
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bioRxiv - Biophysics 2023Quote: ... and 2 μg/mL recombinant human CCL2 (Biolegend).
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bioRxiv - Bioengineering 2023Quote: ... 100 ng/ml recombinant human IGF-1 (BioLegend), 50 ng/ml recombinant human FGF-2 (Peprotech) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 ng/ml recombinant human IGF-1 (BioLegend), 50 ng/ml recombinant human FGF-2 (Peprotech) ...
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bioRxiv - Immunology 2024Quote: ... plus 20 ng/mL recombinant human BAFF (Biolegend) for 3 days ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
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bioRxiv - Cancer Biology 2019Quote: ... and anti-human mAb CD4–FITC clone RPA-T4 (BioLegend, CA). The flow cytometry data analysis was carried out using FlowJo®.
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 700 mouse anti-human CD4 (Biolegend, catalog no. 300526), and APC-H7 mouse anti-human CD19 (BD biosciences ...
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bioRxiv - Immunology 2020Quote: ... PE/Dazzle™ 594 anti-human CD4 (Clone RPA-T4, Biolegend), Alexa Fluor 700 anti-human CD8 (Clone SK1 ...
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bioRxiv - Immunology 2022Quote: ... 1:100-diluted anti-human CD4-PE (BioLegend, clone RPA-T4), and 1:100 diluted anti-human CD8-APC (BioLegend ...
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bioRxiv - Genomics 2023Quote: ... cells were stained with APC anti-human CD4 Antibody (Biolegend, 357408), diluted 1:100 for 20-30 minutes on ice.
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 488 anti-human CD4 (1:200 dilution, BioLegend, 344618), APC/Fire™ 750 anti-human CD8a (1:50 dilution ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor 647 anti-human CD4 (clone: RPA-T4; BioLegend®), and PE-Cyanine7 anti-human CD8 (Clone ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained with BV510 anti-human CD4 (BioLegend, 357420) at 1:200 dilution on ice for 5 min ...
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bioRxiv - Immunology 2019Quote: ... and 5 ng/mL recombinant human IL-15 (Biolegend). Before use ...
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bioRxiv - Cancer Biology 2021Quote: ... and 100 IU/mL recombinant human IL-2 (Biolegend) in RPMI media supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... recombinant PE anti-human/mouse TLR2 (clone QA16A01; Biolegend), FITC anti-mouse CD14 (clone Sa14-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/mL recombinant human thrombopoietin (hTPO) (BioLegend #597402), DNase (100 μg/mL final concentration) ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD4 BV510 (1:100, clone RPA-T4, BioLegend, cat # 300546), Anti-human CD56 BV605 (1:50 ...
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bioRxiv - Genomics 2023Quote: ... Antibodies used were APC anti-human CD4 clone RPA-T4 (Biolegend, 300514), APC anti-human CD81 clone 5A6 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... rat anti-human CD4 conjugated to Alexa Fluor 700 (A161A1, Biolegend, 357417), eBioscience Fixable Viability Dye conjugated to eFluor 450 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Blood CD4+ T cells were isolated from healthy donor or patient PBMCs using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation ...
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bioRxiv - Immunology 2020Quote: ... and 50 ng/ml recombinant human M-CSF (BioLegend #574806) for 6 days to obtain monocytes derived macrophages ...
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bioRxiv - Immunology 2021Quote: ... 5ng/ml or 50ng/ml recombinant human G-CSF (BioLegend) or mouse G-CSF (BioLegend) ...
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bioRxiv - Microbiology 2022Quote: ... and FITC anti-human/mouse Granzyme B recombinant antibody (Biolegend). Samples were analyzed on the CytoFLEX LX flow cytometer (Beckman Coulter).
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bioRxiv - Cancer Biology 2022Quote: ... Human and mouse recombinant IFNα/β/γ(all from Biolegend) were used at a concentration of 100ng/ml.
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bioRxiv - Immunology 2022Quote: ... CD4 (anti-CD4-FITC; BioLegend), CD8 (anti-CD8-PeCy7 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained with APC/Cy7 anti-human CD4 (clone RPA-T4, Biolegend) and AlexaFluor647 anti-mouse H-2Kd (clone SF1-1.1 ...
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bioRxiv - Immunology 2021Quote: ... For control staining PE/Cy5-labeled anti-human CD4 antibody (RPA-T4, Biolegend) was used ...
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bioRxiv - Cancer Biology 2022Quote: ... APC anti-human CD4 Antibody (#357408, Rat IgG2b κ, dilution 1:20, Biolegend); FITC anti-human CD8 Antibody (#344704 ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with the following Abs for flow cytometry: human CD4 (BioLegend, #300532), human CD3 (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... CD4+ T cells were isolated from the PBMC population by immunomagnetic positive selection using the MojoSort Human CD4 Nanobeads (BioLegend, San Diego, CA) and an EasySep Magnet (STEMCELL™ Technologies Inc. ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... CD4+ T cells and CD8+ T cells were separated using Biolegend MojoSort Human CD4 T Cell Isolation Kit (Biolegend #480130, San Diego, CA) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Recombinant human ARG1 was obtained from Biolegend (San Diego, CA, USA), recombinant murine Arg1 was obtained from Cloud-Clone Corp. ...
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bioRxiv - Immunology 2021Quote: ... and mouse recombinant PE anti-human/mouse TLR2 (clone QA16A01; Biolegend) were used.
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bioRxiv - Immunology 2022Quote: ... and recombinant human transforming growth factor beta-1 (TGF-β1; Biolegend) were used at final concentrations of 10 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... and 1 U/mL of recombinant human IL-2 (BioLegend, #589102) in 200 μL of complete culture medium (see above for media components) ...
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bioRxiv - Immunology 2023Quote: ... were coated with 1.54 μg/mL human recombinant VCAM-1 (Biolegend) and/or 1.14 μg/mL human recombinant ICAM-1 (R&D system ...
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bioRxiv - Bioengineering 2023Quote: ... and 500 IU/ml of recombinant human IL-2 (Biolegend, 589104). Stimulated T cells were co-transduced via spinoculation at 931xg ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were treated with TNF-α recombinant human protein (Biolegend) at a concentration of 75 ng/ml for 24 h in combination with cycloheximide (CHX ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1000 U/ml of human recombinant IFN-γ (BioLegend), 20 ng/ml of EGF (Sigma ...